Restriction map for
pF1KB8684
(Circular) (Six-base) MAPSORT of: pF1KB8684.seq Check: 4053 from: 1 to: 4250
pF1KB8684 4250 bp
With 182 enzymes: SgfI *
July 15, 2008 09:38 ..
AccI GT'mk_AC
Cuts at: 1288 1288
Size: 4250
Acc65I G'GTAC_C
Cuts at: 1266 1266
Size: 4250
AclI AA'CG_TT
Cuts at: 425 4090 425
Size: 3665 585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 2075 2507 2860 3522 542
Size: 1533 432 353 662 1270
Fragments arranged by size:
1533 1270 662 432 353
AflIII A'CryG_T
Cuts at: 900 1602 3402 3683 900
Size: 702 1800 281 1467
Fragments arranged by size:
1800 1467 702 281
AhdI GACnn_n'nnGTC
Cuts at: 373 373
Size: 4250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 428 428
Size: 4250
AlwNI CAG_nnn'CTG
Cuts at: 188 1555 2993 188
Size: 1367 1438 1445
Fragments arranged by size:
1445 1438 1367
ApaLI G'TGCA_C
Cuts at: 3088 3088
Size: 4250
ApoI r'AATT_y
Cuts at: 445 1030 1254 3603 3859 445
Size: 585 224 2349 256 836
Fragments arranged by size:
2349 836 585 256 224
AseI AT'TA_AT
Cuts at: 20 358 20
Size: 338 3912
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
AvaI C'yCGr_G
Cuts at: 1270 1270
Size: 4250
BamHI G'GATC_C
Cuts at: 1275 1275
Size: 4250
BanI G'GyrC_C
Cuts at: 1266 1680 1928 1963 1266
Size: 414 248 35 3553
Fragments arranged by size:
3553 414 248 35
BanII G_rGCy'C
Cuts at: 1264 2294 1264
Size: 1030 3220
BbeI G_GCGC'C
Cuts at: 1684 1932 1684
Size: 248 4002
BbsI GAAGACnn'nnnn_
Cuts at: 1010 1010
Size: 4250
BciVI GTATCCnnnnn_n'
Cuts at: 2148 3204 4250 2148
Size: 1056 1046 2148
Fragments arranged by size:
2148 1056 1046
BclI T'GATC_A
Cuts at: 1770 1770
Size: 4250
BfrBI ATG'CAT
Cuts at: 130 260 130
Size: 130 4120
BglII A'GATC_T
Cuts at: 1765 3802 1765
Size: 2037 2213
BlpI GC'TnA_GC
Cuts at: 1362 1362
Size: 4250
Bme1580I G_kGCm'C
Cuts at: 1875 1968 3092 1875
Size: 93 1124 3033
Fragments arranged by size:
3033 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 571 1462 1543 1878 4022 571
Size: 891 81 335 2144 799
Fragments arranged by size:
2144 891 799 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 4250
Bpu10I CC'TnA_GC
Cuts at: 1102 1102
Size: 4250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 820 1426 2305 3019 3317 735
Size: 85 606 879 714 298 1668
Fragments arranged by size:
1668 879 714 606 298 85
BsaI GGTCTCn'nnnn_
Cuts at: 360 3954 360
Size: 3594 656
BsaAI yAC'GTr
Cuts at: 252 1603 252
Size: 1351 2899
BsaBI GATnn'nnATC
Cuts at: 6 1145 1764 3801 6
Size: 1139 619 2037 455
Fragments arranged by size:
2037 1139 619 455
BsaHI Gr'CG_yC
Cuts at: 635 1681 1929 2631 4127 635
Size: 1046 248 702 1496 758
Fragments arranged by size:
1496 1046 758 702 248
BsaWI w'CCGG_w
Cuts at: 1439 1960 2638 3049 3196 3470 1439
Size: 521 678 411 147 274 2219
Fragments arranged by size:
2219 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 554 584 554
Size: 30 4220
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 864 864
Size: 4250
BseYI C'CCAG_C
Cuts at: 1675 2433 3098 3506 1675
Size: 758 665 408 2419
Fragments arranged by size:
2419 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1792 1838 3068 104
Size: 1688 46 1230 1286
Fragments arranged by size:
1688 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1264 2042 2232 3092 1264
Size: 778 190 860 2422
Fragments arranged by size:
2422 860 778 190
BsmI GAATG_Cn'
Cuts at: 199 199
Size: 4250
Bsp1286I G_dGCh'C
Cuts at: 1264 1875 1968 2042 2232 2294 3092 1264
Size: 611 93 74 190 62 798 2422
Fragments arranged by size:
2422 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2682 2682
Size: 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1300 1514 1816 2197 1300
Size: 214 302 381 3353
Fragments arranged by size:
3353 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2542 2596 4080 4245 50
Size: 2492 54 1484 165 55
Fragments arranged by size:
2492 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 132 2162 132
Size: 2030 2220
BsrFI r'CCGG_y
Cuts at: 2248 2429 2248
Size: 181 4069
BssSI C'ACGA_G
Cuts at: 2521 3229 2521
Size: 708 3542
BstAPI GCAn_nnn'nTGC
Cuts at: 212 1627 212
Size: 1415 2835
BstBI TT'CG_AA
Cuts at: 2611 2611
Size: 4250
BstYI r'GATC_y
Cuts at: 1 1153 1275 1742 1765 2100 2346 2652 2664
Size: 1152 122 467 23 335 246 306 12
Cuts at: 2664 2750 3802 1
Size: 86 1052 449
Fragments arranged by size:
1152 1052 467 449 335 306 246 122
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 824 986 824
Size: 162 4088
BtgI C'CryG_G
Cuts at: 107 638 107
Size: 531 3719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 248 2200 2381 2544 248
Size: 1952 181 163 1954
Fragments arranged by size:
1954 1952 181 163
BtsI GCAGTG_nn'
Cuts at: 1493 1493
Size: 4250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 114 149 114
Size: 35 4215
DraI TTT'AAA
Cuts at: 473 1249 473
Size: 776 3474
DraIII CAC_nnn'GTG
Cuts at: 579 579
Size: 4250
DrdI GACnn_nn'nnGTC
Cuts at: 1956 3300 1956
Size: 1344 2906
EaeI y'GGCC_r
Cuts at: 565 1835 2009 2400 2427 3772 565
Size: 1270 174 391 27 1345 1043
Fragments arranged by size:
1345 1270 1043 391 174 27
EagI C'GGCC_G
Cuts at: 1835 1835
Size: 4250
EarI CTCTTCn'nnn_
Cuts at: 841 887 910 1154 2273 2483 841
Size: 46 23 244 1119 210 2608
Fragments arranged by size:
2608 1119 244 210 46 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1007 2458 3200 3346 4060 1007
Size: 1451 742 146 714 1197
Fragments arranged by size:
1451 1197 742 714 146
EcoICRI GAG'CTC
Cuts at: 1262 1262
Size: 4250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 2075 2507 2860 3522 542
Size: 1533 432 353 662 1270
Fragments arranged by size:
1533 1270 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1022 1022
Size: 4250
EcoO109I rG'GnC_Cy
Cuts at: 1071 1389 1071
Size: 318 3932
EcoRI G'AATT_C
Cuts at: 1254 1254
Size: 4250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1252 2269 1252
Size: 1017 3233
FspI TGC'GCA
Cuts at: 2031 2031
Size: 4250
HaeII r_GCGC'y
Cuts at: 884 1684 1932 3162 3933 884
Size: 800 248 1230 771 1201
Fragments arranged by size:
1230 1201 800 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 359 391 2092 2122 2124 2154 359
Size: 32 1701 30 2 30 2455
Fragments arranged by size:
2455 1701 32 30 30 2
HincII GTy'rAC
Cuts at: 1289 1289
Size: 4250
HindIII A'AGCT_T
Cuts at: 524 1234 524
Size: 710 3540
KasI G'GCGC_C
Cuts at: 1680 1928 1680
Size: 248 4002
KpnI G_GTAC'C
Cuts at: 1270 1270
Size: 4250
MluI A'CGCG_T
Cuts at: 900 3683 900
Size: 2783 1467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 3009 3193 554
Size: 2455 184 1611
Fragments arranged by size:
2455 1611 184
MscI TGG'CCA
Cuts at: 2011 2011
Size: 4250
MslI CAynn'nnrTG
Cuts at: 2239 2366 2239
Size: 127 4123
MspA1I CmG'CkG
Cuts at: 1361 1675 2035 2819 3064 1361
Size: 314 360 784 245 2547
Fragments arranged by size:
2547 784 360 314 245
NaeI GCC'GGC
Cuts at: 2431 2431
Size: 4250
NarI GG'CG_CC
Cuts at: 1681 1929 1681
Size: 248 4002
NcoI C'CATG_G
Cuts at: 107 107
Size: 4250
NgoMIV G'CCGG_C
Cuts at: 2429 2429
Size: 4250
NsiI A_TGCA'T
Cuts at: 132 262 132
Size: 130 4120
NspI r_CATG'y
Cuts at: 125 134 172 595 1303 1803 3406 125
Size: 9 38 423 708 500 1603 969
Fragments arranged by size:
1603 969 708 500 423 38 9
PciI A'CATG_T
Cuts at: 3402 3402
Size: 4250
PflMI CCAn_nnn'nTGG
Cuts at: 579 579
Size: 4250
PmeI GTTT'AAAC
Cuts at: 1249 1249
Size: 4250
PmlI CAC'GTG
Cuts at: 252 252
Size: 4250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 4218
PstI C_TGCA'G
Cuts at: 843 1297 1982 843
Size: 454 685 3111
Fragments arranged by size:
3111 685 454
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4250
PvuII CAG'CTG
Cuts at: 1675 2035 1675
Size: 360 3890
RsrII CG'GwC_CG
Cuts at: 2445 2445
Size: 4250
SacI G_AGCT'C
Cuts at: 1264 1264
Size: 4250
SalI G'TCGA_C
Cuts at: 1287 1287
Size: 4250
SapI GCTCTTCn'nnn_
Cuts at: 2273 2483 2273
Size: 210 4040
SbfI CC_TGCA'GG
Cuts at: 1297 1297
Size: 4250
ScaI AGT'ACT
Cuts at: 1126 3767 1126
Size: 2641 1609
SexAI A'CCwGG_T
Cuts at: 500 500
Size: 4250
SfcI C'TryA_G
Cuts at: 33 839 1293 1978 2946 3137 33
Size: 806 454 685 968 191 1146
Fragments arranged by size:
1146 968 806 685 454 191
SfoI GGC'GCC
Cuts at: 1682 1930 1682
Size: 248 4002
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
SmaI CCC'GGG
Cuts at: 1272 1272
Size: 4250
SmlI C'TyrA_G
Cuts at: 750 799 1405 2320 3034 3296 750
Size: 49 606 915 714 262 1704
Fragments arranged by size:
1704 915 714 606 262 49
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4250
SphI G_CATG'C
Cuts at: 1303 1803 1303
Size: 500 3750
StyI C'CwwG_G
Cuts at: 107 608 1384 1738 107
Size: 501 776 354 2619
Fragments arranged by size:
2619 776 501 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 590 1833 1966 2425 2634 590
Size: 1243 133 459 209 2206
Fragments arranged by size:
2206 1243 459 209 133
TatI w'GTAC_w
Cuts at: 1124 3765 1124
Size: 2641 1609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 597 629 3767 3799 490
Size: 32 75 32 3138 32 941
Fragments arranged by size:
3138 941 75 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1228 2047 1228
Size: 819 3431
XbaI T'CTAG_A
Cuts at: 1281 1281
Size: 4250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1745 1745
Size: 4250
XmaI C'CCGG_G
Cuts at: 1270 1270
Size: 4250
XmnI GAAnn'nnTTC
Cuts at: 491 491
Size: 4250
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI