Restriction map for
pF1KB8685
(Circular) (Six-base) MAPSORT of: pF1KB8685.seq Check: 4568 from: 1 to: 5264
pF1KB8685 5264 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 1712 2302 1712
Size: 590 4674
Acc65I G'GTAC_C
Cuts at: 2280 2280
Size: 5264
AclI AA'CG_TT
Cuts at: 5104 5104
Size: 5264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 610 986 3089 3521 4268 4536 602
Size: 8 376 2103 432 747 268 1330
Fragments arranged by size:
2103 1330 747 432 376 268 8
AflIII A'CryG_T
Cuts at: 2616 3720 4697 2616
Size: 1104 977 3183
Fragments arranged by size:
3183 1104 977
AgeI A'CCGG_T
Cuts at: 3652 3652
Size: 5264
AhdI GACnn_n'nnGTC
Cuts at: 816 816
Size: 5264
AleI CACnn'nnGTG
Cuts at: 662 1774 662
Size: 1112 4152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1714 1714
Size: 5264
AlwNI CAG_nnn'CTG
Cuts at: 213 507 1952 2172 2569 4136 213
Size: 294 1445 220 397 1567 1341
Fragments arranged by size:
1567 1445 1341 397 294 220
ApaI G_GGCC'C
Cuts at: 1557 1557
Size: 5264
ApaLI G'TGCA_C
Cuts at: 4034 4034
Size: 5264
ApoI r'AATT_y
Cuts at: 1189 2268 4617 4873 1189
Size: 1079 2349 256 1580
Fragments arranged by size:
2349 1580 1079 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
AvaI C'yCGr_G
Cuts at: 226 682 895 1527 1996 2068 2284 226
Size: 456 213 632 469 72 216 3206
Fragments arranged by size:
3206 632 469 456 216 213 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2095 2128 2095
Size: 33 5231
BamHI G'GATC_C
Cuts at: 2289 2289
Size: 5264
BanI G'GyrC_C
Cuts at: 586 672 1372 1920 2280 2694 2942 2977 586
Size: 86 700 548 360 414 248 35 2873
Fragments arranged by size:
2873 700 548 414 360 248 86 35
BanII G_rGCy'C
Cuts at: 449 530 683 1066 1557 2278 3308 449
Size: 81 153 383 491 721 1030 2405
Fragments arranged by size:
2405 1030 721 491 383 153 81
BbeI G_GCGC'C
Cuts at: 1376 2698 2946 1376
Size: 1322 248 3694
Fragments arranged by size:
3694 1322 248
BbsI GAAGACnn'nnnn_
Cuts at: 321 1798 321
Size: 1477 3787
BbvCI CC'TCA_GC
Cuts at: 338 407 751 338
Size: 69 344 4851
Fragments arranged by size:
4851 344 69
BciVI GTATCCnnnnn_n'
Cuts at: 3162 3923 5264 3162
Size: 761 1341 3162
Fragments arranged by size:
3162 1341 761
BclI T'GATC_A
Cuts at: 2784 2784
Size: 5264
BglI GCCn_nnn'nGGC
Cuts at: 1370 1598 1370
Size: 228 5036
BglII A'GATC_T
Cuts at: 2779 4816 2779
Size: 2037 3227
BlpI GC'TnA_GC
Cuts at: 2376 2376
Size: 5264
Bme1580I G_kGCm'C
Cuts at: 591 669 1013 1557 2889 2982 4038 591
Size: 78 344 544 1332 93 1056 1817
Fragments arranged by size:
1817 1332 1056 544 344 93 78
BmgBI CAC'GTC
Cuts at: 1437 1437
Size: 5264
BmrI ACTGGGnnnn_n'
Cuts at: 526 1737 2111 2476 2557 2892 5036 526
Size: 1211 374 365 81 335 2144 754
Fragments arranged by size:
2144 1211 754 374 365 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 185 1383 3631 185
Size: 1198 2248 1818
Fragments arranged by size:
2248 1818 1198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 460 712 847 1427 1723 233
Size: 227 252 135 580 296 3774
Fragments arranged by size:
3774 580 296 252 227 135
Bpu10I CC'TnA_GC
Cuts at: 131 206 338 407 677 751 1060 1368 131
Size: 75 132 69 270 74 309 308 4027
Fragments arranged by size:
4027 309 308 270 132 75 74 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2440 3319 3811 4109 2440
Size: 879 492 298 3595
Fragments arranged by size:
3595 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 107 4968 107
Size: 4861 403
BsaAI yAC'GTr
Cuts at: 2617 2617
Size: 5264
BsaBI GATnn'nnATC
Cuts at: 6 2778 4815 6
Size: 2772 2037 455
Fragments arranged by size:
2772 2037 455
BsaHI Gr'CG_yC
Cuts at: 254 1052 1373 2695 2943 3645 5141 254
Size: 798 321 1322 248 702 1496 377
Fragments arranged by size:
1496 1322 798 702 377 321 248
BsaWI w'CCGG_w
Cuts at: 2453 2974 3652 3926 4073 4484 2453
Size: 521 678 274 147 411 3233
Fragments arranged by size:
3233 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 1000 1003 1219 1222 1231 1234 1237 1246
Size: 756 3 216 3 9 3 3 9
Cuts at: 1246 1288 1291 1300 1309 1372 1633 1636 1648
Size: 42 3 9 9 63 261 3 12
Cuts at: 1648 1651 1684 1813 1816 1819 1834 1864 2176
Size: 3 33 129 3 3 15 30 312
Cuts at: 2176 2224 2227 2230 2233 2242 2245 2248 2251
Size: 48 3 3 3 9 3 3 3
Cuts at: 2251 2260 244
Size: 9 3248
Fragments arranged by size:
3248 756 312 261 216 129 63 48
42 33 30 15 12 9 9 9
9 9 9 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 143 257 351 590 645 1596 1651 2048 2689
Size: 114 94 239 55 951 55 397 641
Cuts at: 2689 3447 4024 4520 143
Size: 758 577 496 887
Fragments arranged by size:
951 887 758 641 577 496 397 239
114 94 55 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1752 724
Size: 1028 4236
BsiEI CG_ry'CG
Cuts at: 104 1577 2806 2852 4060 104
Size: 1473 1229 46 1208 1308
Fragments arranged by size:
1473 1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 449 767 1066 2278 3056 3246 4038 449
Size: 318 299 1212 778 190 792 1675
Fragments arranged by size:
1675 1212 792 778 318 299 190
Bsp1286I G_dGCh'C
Cuts at: 449 530 591 669 683 767 1013 1066 1557
Size: 81 61 78 14 84 246 53 491
Cuts at: 1557 2278 2889 2982 3056 3246 3308 4038 449
Size: 721 611 93 74 190 62 730 1675
Fragments arranged by size:
1675 730 721 611 491 246 190 93
84 81 78 74 62 61 53 14
BspEI T'CCGG_A
Cuts at: 4484 4484
Size: 5264
BspHI T'CATG_A
Cuts at: 4440 4440
Size: 5264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 125 426 731 1556 1575 2314 2528 2830 3211
Size: 301 305 825 19 739 214 302 381
Cuts at: 3211 125
Size: 2178
Fragments arranged by size:
2178 825 739 381 305 302 301 214
19
BsrBI CCG'CTC
Cuts at: 50 1367 3556 3610 5094 5259 50
Size: 1317 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 1317 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3176 3176
Size: 5264
BsrFI r'CCGG_y
Cuts at: 1048 3262 3443 3652 1048
Size: 2214 181 209 2660
Fragments arranged by size:
2660 2214 209 181
BssSI C'ACGA_G
Cuts at: 3535 3893 3535
Size: 358 4906
BstAPI GCAn_nnn'nTGC
Cuts at: 743 1752 1952 2641 743
Size: 1009 200 689 3366
Fragments arranged by size:
3366 1009 689 200
BstBI TT'CG_AA
Cuts at: 3625 3625
Size: 5264
BstXI CCAn_nnnn'nTGG
Cuts at: 1020 1020
Size: 5264
BstYI r'GATC_y
Cuts at: 1 788 1148 2289 2756 2779 3114 3360 4372
Size: 787 360 1141 467 23 335 246 1012
Cuts at: 4372 4458 4470 4816 1
Size: 86 12 346 449
Fragments arranged by size:
1141 1012 787 467 449 360 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 806 806
Size: 5264
BtgI C'CryG_G
Cuts at: 107 1304 1417 1718 107
Size: 1197 113 301 3653
Fragments arranged by size:
3653 1197 301 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3214 3395 3558 3214
Size: 181 163 4920
Fragments arranged by size:
4920 181 163
BtsI GCAGTG_nn'
Cuts at: 856 1876 2507 856
Size: 1020 631 3613
Fragments arranged by size:
3613 1020 631
DraI TTT'AAA
Cuts at: 2263 2263
Size: 5264
DraIII CAC_nnn'GTG
Cuts at: 851 851
Size: 5264
DrdI GACnn_nn'nnGTC
Cuts at: 1504 2970 3828 1504
Size: 1466 858 2940
Fragments arranged by size:
2940 1466 858
EaeI y'GGCC_r
Cuts at: 698 1091 1178 1574 1721 1970 2849 3023 3414
Size: 393 87 396 147 249 879 174 391
Cuts at: 3414 3441 4786 698
Size: 27 1345 1176
Fragments arranged by size:
1345 1176 879 396 393 391 249 174
147 87 27
EagI C'GGCC_G
Cuts at: 1574 2849 1574
Size: 1275 3989
EarI CTCTTCn'nnn_
Cuts at: 1820 2153 3287 3497 1820
Size: 333 1134 210 3587
Fragments arranged by size:
3587 1134 333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 937 3472 3782 3928 5074 937
Size: 2535 310 146 1146 1127
Fragments arranged by size:
2535 1146 1127 310 146
EcoICRI GAG'CTC
Cuts at: 447 1064 2276 447
Size: 617 1212 3435
Fragments arranged by size:
3435 1212 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 460 602 610 712 847 986 1427 1723
Size: 227 142 8 102 135 139 441 296
Cuts at: 1723 3089 3521 4268 4536 233
Size: 1366 432 747 268 961
Fragments arranged by size:
1366 961 747 441 432 296 268 227
142 139 135 102 8
EcoNI CCTnn'n_nnAGG
Cuts at: 180 1756 1816 180
Size: 1576 60 3628
Fragments arranged by size:
3628 1576 60
EcoO109I rG'GnC_Cy
Cuts at: 210 810 1809 2000 2403 210
Size: 600 999 191 403 3071
Fragments arranged by size:
3071 999 600 403 191
EcoRI G'AATT_C
Cuts at: 1189 2268 1189
Size: 1079 4185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1497 3283 1497
Size: 1786 3478
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5264
HaeII r_GCGC'y
Cuts at: 138 940 1376 2698 2946 3968 4947 138
Size: 802 436 1322 248 1022 979 455
Fragments arranged by size:
1322 1022 979 802 455 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 562 594 802 834 3106 3136 3138
Size: 32 175 32 208 32 2272 30 2
Cuts at: 3138 3168 355
Size: 30 2451
Fragments arranged by size:
2451 2272 208 175 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 537 1713 2303 537
Size: 1176 590 3498
Fragments arranged by size:
3498 1176 590
KasI G'GCGC_C
Cuts at: 1372 2694 2942 1372
Size: 1322 248 3694
Fragments arranged by size:
3694 1322 248
KpnI G_GTAC'C
Cuts at: 2284 2284
Size: 5264
MfeI C'AATT_G
Cuts at: 741 741
Size: 5264
MluI A'CGCG_T
Cuts at: 4697 4697
Size: 5264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2009 3935 4119 2009
Size: 1926 184 3154
Fragments arranged by size:
3154 1926 184
MscI TGG'CCA
Cuts at: 1093 1723 3025 1093
Size: 630 1302 3332
Fragments arranged by size:
3332 1302 630
MslI CAynn'nnrTG
Cuts at: 662 1696 1774 2137 3253 3380 662
Size: 1034 78 363 1116 127 2546
Fragments arranged by size:
2546 1116 1034 363 127 78
MspA1I CmG'CkG
Cuts at: 220 471 801 1104 1596 1766 2375 2689 3049
Size: 251 330 303 492 170 609 314 360
Cuts at: 3049 4062 4307 220
Size: 1013 245 1177
Fragments arranged by size:
1177 1013 609 492 360 330 314 303
251 245 170
NaeI GCC'GGC
Cuts at: 1050 3445 1050
Size: 2395 2869
NarI GG'CG_CC
Cuts at: 1373 2695 2943 1373
Size: 1322 248 3694
Fragments arranged by size:
3694 1322 248
NcoI C'CATG_G
Cuts at: 107 1417 1718 107
Size: 1310 301 3653
Fragments arranged by size:
3653 1310 301
NgoMIV G'CCGG_C
Cuts at: 1048 3443 1048
Size: 2395 2869
NspI r_CATG'y
Cuts at: 2317 2817 3724 2317
Size: 500 907 3857
Fragments arranged by size:
3857 907 500
PasI CC'CwG_GG
Cuts at: 2196 2196
Size: 5264
PciI A'CATG_T
Cuts at: 3720 3720
Size: 5264
PflMI CCAn_nnn'nTGG
Cuts at: 507 851 1991 507
Size: 344 1140 3780
Fragments arranged by size:
3780 1140 344
PfoI T'CCnGG_A
Cuts at: 521 824 1193 521
Size: 303 369 4592
Fragments arranged by size:
4592 369 303
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 4403
Size: 32 5232
PpuMI rG'GwC_Cy
Cuts at: 810 1809 810
Size: 999 4265
PspOMI G'GGCC_C
Cuts at: 1553 1553
Size: 5264
PstI C_TGCA'G
Cuts at: 470 731 1948 2311 2996 470
Size: 261 1217 363 685 2738
Fragments arranged by size:
2738 1217 685 363 261
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5264
PvuII CAG'CTG
Cuts at: 1104 2689 3049 1104
Size: 1585 360 3319
Fragments arranged by size:
3319 1585 360
RsrII CG'GwC_CG
Cuts at: 3459 3459
Size: 5264
SacI G_AGCT'C
Cuts at: 449 1066 2278 449
Size: 617 1212 3435
Fragments arranged by size:
3435 1212 617
SalI G'TCGA_C
Cuts at: 1711 2301 1711
Size: 590 4674
SapI GCTCTTCn'nnn_
Cuts at: 3287 3497 3287
Size: 210 5054
SbfI CC_TGCA'GG
Cuts at: 2311 2311
Size: 5264
ScaI AGT'ACT
Cuts at: 1963 4781 1963
Size: 2818 2446
SexAI A'CCwGG_T
Cuts at: 767 1172 767
Size: 405 4859
SfcI C'TryA_G
Cuts at: 33 466 660 727 1944 2307 2992 3985 4176
Size: 433 194 67 1217 363 685 993 191
Cuts at: 4176 33
Size: 1121
Fragments arranged by size:
1217 1121 993 685 433 363 194 191
67
SfoI GGC'GCC
Cuts at: 1374 2696 2944 1374
Size: 1322 248 3694
Fragments arranged by size:
3694 1322 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
SmaI CCC'GGG
Cuts at: 1529 1998 2286 1529
Size: 469 288 4507
Fragments arranged by size:
4507 469 288
SmlI C'TyrA_G
Cuts at: 2419 3334 3826 4088 2419
Size: 915 492 262 3595
Fragments arranged by size:
3595 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5264
SphI G_CATG'C
Cuts at: 2317 2817 2317
Size: 500 4764
StuI AGG'CCT
Cuts at: 175 718 175
Size: 543 4721
StyI C'CwwG_G
Cuts at: 107 542 713 1352 1417 1718 2398 2752 107
Size: 435 171 639 65 301 680 354 2619
Fragments arranged by size:
2619 680 639 435 354 301 171 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 343 1719 1764 2847 2980 3439 3648 343
Size: 1376 45 1083 133 459 209 1959
Fragments arranged by size:
1959 1376 1083 459 209 133 45
TatI w'GTAC_w
Cuts at: 1388 1961 2060 4779 1388
Size: 573 99 2719 1873
Fragments arranged by size:
2719 1873 573 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 372 404 1733 1765 4781 4813 372
Size: 32 1329 32 3016 32 823
Fragments arranged by size:
3016 1329 823 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1312 3061 1312
Size: 1749 3515
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 915 1101 2759 915
Size: 186 1658 3420
Fragments arranged by size:
3420 1658 186
XmaI C'CCGG_G
Cuts at: 1527 1996 2284 1527
Size: 469 288 4507
Fragments arranged by size:
4507 469 288
XmnI GAAnn'nnTTC
Cuts at: 1480 1480
Size: 5264
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BcgI
BfrBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI