Restriction map for
pF1KB8686
(Circular) (Six-base) MAPSORT of: pF1KB8686.seq Check: 1239 from: 1 to: 4325
pF1KB8686 4325 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1363 1363
Size: 4325
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 4165 4165
Size: 4325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1223 2150 2582 2935 3597 1223
Size: 927 432 353 662 1951
Fragments arranged by size:
1951 927 662 432 353
AflIII A'CryG_T
Cuts at: 911 1677 3477 3758 911
Size: 766 1800 281 1478
Fragments arranged by size:
1800 1478 766 281
AlwNI CAG_nnn'CTG
Cuts at: 150 1630 3068 150
Size: 1480 1438 1407
Fragments arranged by size:
1480 1438 1407
ApaLI G'TGCA_C
Cuts at: 3163 3163
Size: 4325
ApoI r'AATT_y
Cuts at: 930 1173 1329 3678 3934 930
Size: 243 156 2349 256 1321
Fragments arranged by size:
2349 1321 256 243 156
AseI AT'TA_AT
Cuts at: 20 521 20
Size: 501 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 189 1345 189
Size: 1156 3169
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 1341 1755 2003 2038 1341
Size: 414 248 35 3628
Fragments arranged by size:
3628 414 248 35
BanII G_rGCy'C
Cuts at: 241 1339 2369 241
Size: 1098 1030 2197
Fragments arranged by size:
2197 1098 1030
BbeI G_GCGC'C
Cuts at: 1759 2007 1759
Size: 248 4077
BbsI GAAGACnn'nnnn_
Cuts at: 859 1298 859
Size: 439 3886
BciVI GTATCCnnnnn_n'
Cuts at: 952 2223 3279 4325 952
Size: 1271 1056 1046 952
Fragments arranged by size:
1271 1056 1046 952
BclI T'GATC_A
Cuts at: 1845 1845
Size: 4325
BglII A'GATC_T
Cuts at: 1840 3877 1840
Size: 2037 2288
BlpI GC'TnA_GC
Cuts at: 1437 1437
Size: 4325
Bme1580I G_kGCm'C
Cuts at: 1950 2043 3167 1950
Size: 93 1124 3108
Fragments arranged by size:
3108 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1537 1618 1953 4097 1537
Size: 81 335 2144 1765
Fragments arranged by size:
2144 1765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 791 172
Size: 619 3706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2380 3094 3392 1501
Size: 879 714 298 2434
Fragments arranged by size:
2434 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4029 4029
Size: 4325
BsaAI yAC'GTr
Cuts at: 1678 1678
Size: 4325
BsaBI GATnn'nnATC
Cuts at: 6 257 1839 3876 6
Size: 251 1582 2037 455
Fragments arranged by size:
2037 1582 455 251
BsaHI Gr'CG_yC
Cuts at: 1756 2004 2706 4202 1756
Size: 248 702 1496 1879
Fragments arranged by size:
1879 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1514 2035 2713 3124 3271 3545 1514
Size: 521 678 411 147 274 2294
Fragments arranged by size:
2294 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 189 207 189
Size: 18 4307
BseYI C'CCAG_C
Cuts at: 892 1750 2508 3173 3581 892
Size: 858 758 665 408 1636
Fragments arranged by size:
1636 858 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 878
Size: 4325
BsiEI CG_ry'CG
Cuts at: 104 216 1867 1913 3143 104
Size: 112 1651 46 1230 1286
Fragments arranged by size:
1651 1286 1230 112 46
BsiHKAI G_wGCw'C
Cuts at: 241 798 1339 2117 2307 3167 241
Size: 557 541 778 190 860 1399
Fragments arranged by size:
1399 860 778 557 541 190
Bsp1286I G_dGCh'C
Cuts at: 241 798 1339 1950 2043 2117 2307 2369 3167
Size: 557 541 611 93 74 190 62 798
Cuts at: 3167 241
Size: 1399
Fragments arranged by size:
1399 798 611 557 541 190 93 74
62
BspHI T'CATG_A
Cuts at: 2757 2757
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 174 1375 1589 1891 2272 174
Size: 1201 214 302 381 2227
Fragments arranged by size:
2227 1201 381 302 214
BsrBI CCG'CTC
Cuts at: 50 156 197 2617 2671 4155 4320 50
Size: 106 41 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 106 55 54 41
BsrDI GCAATG_nn'
Cuts at: 974 2237 974
Size: 1263 3062
BsrFI r'CCGG_y
Cuts at: 2323 2504 2323
Size: 181 4144
BssSI C'ACGA_G
Cuts at: 2596 3304 2596
Size: 708 3617
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4325
BstBI TT'CG_AA
Cuts at: 256 2686 256
Size: 2430 1895
BstYI r'GATC_y
Cuts at: 1 896 1350 1817 1840 2175 2421 2727 2739
Size: 895 454 467 23 335 246 306 12
Cuts at: 2739 2825 3877 1
Size: 86 1052 449
Fragments arranged by size:
1052 895 467 454 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2275 2456 2619 2275
Size: 181 163 3981
Fragments arranged by size:
3981 181 163
BtsI GCAGTG_nn'
Cuts at: 1069 1568 1069
Size: 499 3826
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 2031 3375 2031
Size: 1344 2981
EaeI y'GGCC_r
Cuts at: 213 1301 1910 2084 2475 2502 3847 213
Size: 1088 609 174 391 27 1345 691
Fragments arranged by size:
1345 1088 691 609 391 174 27
EagI C'GGCC_G
Cuts at: 213 1910 213
Size: 1697 2628
EarI CTCTTCn'nnn_
Cuts at: 1091 1097 1154 2348 2558 1091
Size: 6 57 1194 210 2858
Fragments arranged by size:
2858 1194 210 57 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2533 3275 3421 4135 127
Size: 2406 742 146 714 317
Fragments arranged by size:
2406 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 239 1337 239
Size: 1098 3227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 791 1223 2150 2582 2935 3597 172
Size: 619 432 927 432 353 662 900
Fragments arranged by size:
927 900 662 619 432 432 353
EcoO109I rG'GnC_Cy
Cuts at: 186 1464 186
Size: 1278 3047
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4325
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 129 1759 2007 3237 4008 129
Size: 1630 248 1230 771 446
Fragments arranged by size:
1630 1230 771 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1161 2167 2197 2199 2229 1129
Size: 32 1006 30 2 30 3225
Fragments arranged by size:
3225 1006 32 30 30 2
HincII GTy'rAC
Cuts at: 1262 1364 1262
Size: 102 4223
HindIII A'AGCT_T
Cuts at: 1284 1284
Size: 4325
HpaI GTT'AAC
Cuts at: 1262 1262
Size: 4325
KasI G'GCGC_C
Cuts at: 1755 2003 1755
Size: 248 4077
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 486 3084 3268 486
Size: 2598 184 1543
Fragments arranged by size:
2598 1543 184
MscI TGG'CCA
Cuts at: 1303 2086 1303
Size: 783 3542
MslI CAynn'nnrTG
Cuts at: 2314 2441 2314
Size: 127 4198
MspA1I CmG'CkG
Cuts at: 138 1436 1750 2110 2894 3139 138
Size: 1298 314 360 784 245 1324
Fragments arranged by size:
1324 1298 784 360 314 245
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 4325
NarI GG'CG_CC
Cuts at: 1756 2004 1756
Size: 248 4077
NcoI C'CATG_G
Cuts at: 107 107
Size: 4325
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 4325
NspI r_CATG'y
Cuts at: 1378 1878 3481 1378
Size: 500 1603 2222
Fragments arranged by size:
2222 1603 500
PciI A'CATG_T
Cuts at: 3477 3477
Size: 4325
PflMI CCAn_nnn'nTGG
Cuts at: 150 150
Size: 4325
PfoI T'CCnGG_A
Cuts at: 143 768 143
Size: 625 3700
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 4293
PpuMI rG'GwC_Cy
Cuts at: 186 186
Size: 4325
PsiI TTA'TAA
Cuts at: 763 763
Size: 4325
PstI C_TGCA'G
Cuts at: 134 185 1372 2057 134
Size: 51 1187 685 2402
Fragments arranged by size:
2402 1187 685 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 1750 2110 1750
Size: 360 3965
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 241 1339 241
Size: 1098 3227
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4325
SapI GCTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4325
ScaI AGT'ACT
Cuts at: 3842 3842
Size: 4325
SfcI C'TryA_G
Cuts at: 33 130 181 264 1368 2053 3021 3212 33
Size: 97 51 83 1104 685 968 191 1146
Fragments arranged by size:
1146 1104 968 685 191 97 83 51
SfoI GGC'GCC
Cuts at: 1757 2005 1757
Size: 248 4077
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 1347 1347
Size: 4325
SmlI C'TyrA_G
Cuts at: 1480 2395 3109 3371 1480
Size: 915 714 262 2434
Fragments arranged by size:
2434 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
SspI AAT'ATT
Cuts at: 822 822
Size: 4325
StyI C'CwwG_G
Cuts at: 107 954 1459 1813 107
Size: 847 505 354 2619
Fragments arranged by size:
2619 847 505 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 871 1908 2041 2500 2709 871
Size: 1037 133 459 209 2487
Fragments arranged by size:
2487 1037 459 209 133
TatI w'GTAC_w
Cuts at: 543 903 3840 543
Size: 360 2937 1028
Fragments arranged by size:
2937 1028 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1199 1231 3842 3874 1199
Size: 32 2611 32 1650
Fragments arranged by size:
2611 1650 32 32
Tth111I GACn'n_nGTC
Cuts at: 2122 2122
Size: 4325
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 882 1820 882
Size: 938 3387
XmaI C'CCGG_G
Cuts at: 1345 1345
Size: 4325
XmnI GAAnn'nnTTC
Cuts at: 575 575
Size: 4325
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI