Restriction map for
pF1KB8687
(Circular) (Six-base) MAPSORT of: pF1KB8687.seq Check: 5240 from: 1 to: 5057
pF1KB8687 5057 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 2095 2095
Size: 5057
Acc65I G'GTAC_C
Cuts at: 2073 2073
Size: 5057
AclI AA'CG_TT
Cuts at: 4897 4897
Size: 5057
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2882 3314 4061 4329 1288
Size: 1594 432 747 268 2016
Fragments arranged by size:
2016 1594 747 432 268
AflIII A'CryG_T
Cuts at: 1779 2409 3513 4490 1779
Size: 630 1104 977 2346
Fragments arranged by size:
2346 1104 977 630
AgeI A'CCGG_T
Cuts at: 3445 3445
Size: 5057
AhdI GACnn_n'nnGTC
Cuts at: 1762 1762
Size: 5057
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 481 481
Size: 5057
AlwNI CAG_nnn'CTG
Cuts at: 226 2362 3929 226
Size: 2136 1567 1354
Fragments arranged by size:
2136 1567 1354
ApaLI G'TGCA_C
Cuts at: 3827 3827
Size: 5057
ApoI r'AATT_y
Cuts at: 446 765 2061 4410 4666 446
Size: 319 1296 2349 256 837
Fragments arranged by size:
2349 1296 837 319 256
AseI AT'TA_AT
Cuts at: 20 1916 20
Size: 1896 3161
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
AvaI C'yCGr_G
Cuts at: 179 1082 2077 179
Size: 903 995 3159
Fragments arranged by size:
3159 995 903
BamHI G'GATC_C
Cuts at: 2082 2082
Size: 5057
BanI G'GyrC_C
Cuts at: 265 2073 2487 2735 2770 265
Size: 1808 414 248 35 2552
Fragments arranged by size:
2552 1808 414 248 35
BanII G_rGCy'C
Cuts at: 129 2071 3101 129
Size: 1942 1030 2085
Fragments arranged by size:
2085 1942 1030
BbeI G_GCGC'C
Cuts at: 2491 2739 2491
Size: 248 4809
BbsI GAAGACnn'nnnn_
Cuts at: 1575 1575
Size: 5057
BciVI GTATCCnnnnn_n'
Cuts at: 2955 3716 5057 2955
Size: 761 1341 2955
Fragments arranged by size:
2955 1341 761
BclI T'GATC_A
Cuts at: 2577 2577
Size: 5057
BglII A'GATC_T
Cuts at: 948 2572 4609 948
Size: 1624 2037 1396
Fragments arranged by size:
2037 1624 1396
BlpI GC'TnA_GC
Cuts at: 2169 2169
Size: 5057
Bme1580I G_kGCm'C
Cuts at: 2682 2775 3831 2682
Size: 93 1056 3908
Fragments arranged by size:
3908 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2269 2350 2685 4829 2269
Size: 81 335 2144 2497
Fragments arranged by size:
2497 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3424 3424
Size: 5057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 279
Size: 5057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2233 3112 3604 3902 2012
Size: 221 879 492 298 3167
Fragments arranged by size:
3167 879 492 298 221
BsaI GGTCTCn'nnnn_
Cuts at: 4761 4761
Size: 5057
BsaAI yAC'GTr
Cuts at: 143 1821 2410 143
Size: 1678 589 2790
Fragments arranged by size:
2790 1678 589
BsaBI GATnn'nnATC
Cuts at: 6 626 2571 4608 6
Size: 620 1945 2037 455
Fragments arranged by size:
2037 1945 620 455
BsaHI Gr'CG_yC
Cuts at: 842 2488 2736 3438 4934 842
Size: 1646 248 702 1496 965
Fragments arranged by size:
1646 1496 965 702 248
BsaWI w'CCGG_w
Cuts at: 2246 2767 3445 3719 3866 4277 2246
Size: 521 678 274 147 411 3026
Fragments arranged by size:
3026 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1134 1164 1845 1875 1134
Size: 30 681 30 4316
Fragments arranged by size:
4316 681 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 137 207 220 1966 137
Size: 70 13 1746 3228
Fragments arranged by size:
3228 1746 70 13
BseYI C'CCAG_C
Cuts at: 1030 2482 3240 3817 4313 1030
Size: 1452 758 577 496 1774
Fragments arranged by size:
1774 1452 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 813
Size: 5057
BsiEI CG_ry'CG
Cuts at: 104 360 2599 2645 3853 104
Size: 256 2239 46 1208 1308
Fragments arranged by size:
2239 1308 1208 256 46
BsiHKAI G_wGCw'C
Cuts at: 129 2071 2849 3039 3831 129
Size: 1942 778 190 792 1355
Fragments arranged by size:
1942 1355 792 778 190
BsmI GAATG_Cn'
Cuts at: 538 550 538
Size: 12 5045
Bsp1286I G_dGCh'C
Cuts at: 129 2071 2682 2775 2849 3039 3101 3831 129
Size: 1942 611 93 74 190 62 730 1355
Fragments arranged by size:
1942 1355 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4277 4277
Size: 5057
BspHI T'CATG_A
Cuts at: 4233 4233
Size: 5057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 877 2107 2321 2623 3004 835
Size: 42 1230 214 302 381 2888
Fragments arranged by size:
2888 1230 381 302 214 42
BsrBI CCG'CTC
Cuts at: 50 3349 3403 4887 5052 50
Size: 3299 54 1484 165 55
Fragments arranged by size:
3299 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 470 2969 470
Size: 2499 2558
BsrFI r'CCGG_y
Cuts at: 198 3055 3236 3445 198
Size: 2857 181 209 1810
Fragments arranged by size:
2857 1810 209 181
BssSI C'ACGA_G
Cuts at: 301 3328 3686 301
Size: 3027 358 1672
Fragments arranged by size:
3027 1672 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2434 2434
Size: 5057
BstBI TT'CG_AA
Cuts at: 3418 3418
Size: 5057
BstXI CCAn_nnnn'nTGG
Cuts at: 343 343
Size: 5057
BstYI r'GATC_y
Cuts at: 1 948 1467 2082 2549 2572 2907 3153 4165
Size: 947 519 615 467 23 335 246 1012
Cuts at: 4165 4251 4263 4609 1
Size: 86 12 346 449
Fragments arranged by size:
1012 947 615 519 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 227 860 227
Size: 633 4424
BtgI C'CryG_G
Cuts at: 117 287 1801 117
Size: 170 1514 3373
Fragments arranged by size:
3373 1514 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1396 3007 3188 3351 1396
Size: 1611 181 163 3102
Fragments arranged by size:
3102 1611 181 163
BtsI GCAGTG_nn'
Cuts at: 491 713 2300 491
Size: 222 1587 3248
Fragments arranged by size:
3248 1587 222
DraI TTT'AAA
Cuts at: 1707 2056 1707
Size: 349 4708
DrdI GACnn_nn'nnGTC
Cuts at: 2763 3621 2763
Size: 858 4199
EaeI y'GGCC_r
Cuts at: 290 610 2642 2816 3207 3234 4579 290
Size: 320 2032 174 391 27 1345 768
Fragments arranged by size:
2032 1345 768 391 320 174 27
EagI C'GGCC_G
Cuts at: 2642 2642
Size: 5057
EarI CTCTTCn'nnn_
Cuts at: 325 1181 1514 1839 3080 3290 325
Size: 856 333 325 1241 210 2092
Fragments arranged by size:
2092 1241 856 333 325 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 217 3265 3575 3721 4867 217
Size: 3048 310 146 1146 407
Fragments arranged by size:
3048 1146 407 310 146
EcoICRI GAG'CTC
Cuts at: 127 2069 127
Size: 1942 3115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 1288 2882 3314 4061 4329 279
Size: 1009 1594 432 747 268 1007
Fragments arranged by size:
1594 1009 1007 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 223 275 2196 223
Size: 52 1921 3084
Fragments arranged by size:
3084 1921 52
EcoRI G'AATT_C
Cuts at: 2061 2061
Size: 5057
EcoRV GAT'ATC
Cuts at: 1209 1209
Size: 5057
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 354 714 3076 354
Size: 360 2362 2335
Fragments arranged by size:
2362 2335 360
FspI TGC'GCA
Cuts at: 2838 2838
Size: 5057
HaeII r_GCGC'y
Cuts at: 187 2491 2739 3761 4740 187
Size: 2304 248 1022 979 504
Fragments arranged by size:
2304 1022 979 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 1600 1632 1732 1748 1764 1780 1945
Size: 32 1344 32 100 16 16 16 165
Cuts at: 1945 1977 2899 2929 2931 2961 224
Size: 32 922 30 2 30 2320
Fragments arranged by size:
2320 1344 922 165 100 32 32 32
30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 2096 2096
Size: 5057
KasI G'GCGC_C
Cuts at: 2487 2735 2487
Size: 248 4809
KpnI G_GTAC'C
Cuts at: 2077 2077
Size: 5057
MluI A'CGCG_T
Cuts at: 4490 4490
Size: 5057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 546 579 733 1292 3728 3912 546
Size: 33 154 559 2436 184 1691
Fragments arranged by size:
2436 1691 559 184 154 33
MscI TGG'CCA
Cuts at: 2818 2818
Size: 5057
MslI CAynn'nnrTG
Cuts at: 921 3046 3173 921
Size: 2125 127 2805
Fragments arranged by size:
2805 2125 127
MspA1I CmG'CkG
Cuts at: 295 315 2168 2482 2842 3855 4100 295
Size: 20 1853 314 360 1013 245 1252
Fragments arranged by size:
1853 1252 1013 360 314 245 20
NaeI GCC'GGC
Cuts at: 200 3238 200
Size: 3038 2019
NarI GG'CG_CC
Cuts at: 2488 2736 2488
Size: 248 4809
NgoMIV G'CCGG_C
Cuts at: 198 3236 198
Size: 3038 2019
NspI r_CATG'y
Cuts at: 1065 1539 1783 2110 2610 3517 1065
Size: 474 244 327 500 907 2605
Fragments arranged by size:
2605 907 500 474 327 244
PciI A'CATG_T
Cuts at: 1779 3513 1779
Size: 1734 3323
PfoI T'CCnGG_A
Cuts at: 1224 1224
Size: 5057
PmeI GTTT'AAAC
Cuts at: 2056 2056
Size: 5057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 5025
PpuMI rG'GwC_Cy
Cuts at: 223 223
Size: 5057
PsiI TTA'TAA
Cuts at: 704 704
Size: 5057
PstI C_TGCA'G
Cuts at: 2104 2789 2104
Size: 685 4372
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5057
PvuII CAG'CTG
Cuts at: 2482 2842 2482
Size: 360 4697
RsrII CG'GwC_CG
Cuts at: 3252 3252
Size: 5057
SacI G_AGCT'C
Cuts at: 129 2071 129
Size: 1942 3115
SalI G'TCGA_C
Cuts at: 2094 2094
Size: 5057
SapI GCTCTTCn'nnn_
Cuts at: 325 1181 1839 3080 3290 325
Size: 856 658 1241 210 2092
Fragments arranged by size:
2092 1241 856 658 210
SbfI CC_TGCA'GG
Cuts at: 2104 2104
Size: 5057
ScaI AGT'ACT
Cuts at: 4574 4574
Size: 5057
SfcI C'TryA_G
Cuts at: 33 1549 2100 2785 3778 3969 33
Size: 1516 551 685 993 191 1121
Fragments arranged by size:
1516 1121 993 685 551 191
SfoI GGC'GCC
Cuts at: 2489 2737 2489
Size: 248 4809
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
SmaI CCC'GGG
Cuts at: 2079 2079
Size: 5057
SmlI C'TyrA_G
Cuts at: 2027 2212 3127 3619 3881 2027
Size: 185 915 492 262 3203
Fragments arranged by size:
3203 915 492 262 185
SnaBI TAC'GTA
Cuts at: 1821 1821
Size: 5057
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5057
SphI G_CATG'C
Cuts at: 2110 2610 2110
Size: 500 4557
SspI AAT'ATT
Cuts at: 1123 1809 1920 1123
Size: 686 111 4260
Fragments arranged by size:
4260 686 111
StuI AGG'CCT
Cuts at: 136 136
Size: 5057
StyI C'CwwG_G
Cuts at: 131 2191 2545 131
Size: 2060 354 2643
Fragments arranged by size:
2643 2060 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 2640 2773 3232 3441 346
Size: 2294 133 459 209 1962
Fragments arranged by size:
2294 1962 459 209 133
TatI w'GTAC_w
Cuts at: 391 4572 391
Size: 4181 876
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1134 1166 1845 1877 4574 4606 1134
Size: 32 679 32 2697 32 1585
Fragments arranged by size:
2697 1585 679 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 174 1573 2854 174
Size: 1399 1281 2377
Fragments arranged by size:
2377 1399 1281
XbaI T'CTAG_A
Cuts at: 1750 2088 1750
Size: 338 4719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 562 620 2552 562
Size: 58 1932 3067
Fragments arranged by size:
3067 1932 58
XmaI C'CCGG_G
Cuts at: 2077 2077
Size: 5057
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XhoI XmnI ZraI