Restriction map for
pF1KB8688
(Circular) (Six-base) MAPSORT of: pF1KB8688.seq Check: 2612 from: 1 to: 4619
pF1KB8688 4619 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 213 1657 213
Size: 1444 3175
Acc65I G'GTAC_C
Cuts at: 1635 1635
Size: 4619
AclI AA'CG_TT
Cuts at: 4459 4459
Size: 4619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1474 2444 2876 3229 3891 326
Size: 1148 970 432 353 662 1054
Fragments arranged by size:
1148 1054 970 662 432 353
AflIII A'CryG_T
Cuts at: 1971 3771 4052 1971
Size: 1800 281 2538
Fragments arranged by size:
2538 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1117 1117
Size: 4619
AlwNI CAG_nnn'CTG
Cuts at: 1269 1924 3362 1269
Size: 655 1438 2526
Fragments arranged by size:
2526 1438 655
ApaLI G'TGCA_C
Cuts at: 1152 3457 1152
Size: 2305 2314
ApoI r'AATT_y
Cuts at: 187 378 1005 1504 1623 3972 4228 187
Size: 191 627 499 119 2349 256 578
Fragments arranged by size:
2349 627 578 499 256 191 119
AseI AT'TA_AT
Cuts at: 20 20
Size: 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 506 1598 1639 506
Size: 1092 41 3486
Fragments arranged by size:
3486 1092 41
BamHI G'GATC_C
Cuts at: 1644 1644
Size: 4619
BanI G'GyrC_C
Cuts at: 1635 2049 2297 2332 1635
Size: 414 248 35 3922
Fragments arranged by size:
3922 414 248 35
BanII G_rGCy'C
Cuts at: 1046 1633 2663 1046
Size: 587 1030 3002
Fragments arranged by size:
3002 1030 587
BbeI G_GCGC'C
Cuts at: 2053 2301 2053
Size: 248 4371
BbsI GAAGACnn'nnnn_
Cuts at: 602 845 949 1592 602
Size: 243 104 643 3629
Fragments arranged by size:
3629 643 243 104
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3573 4619 2517
Size: 1056 1046 2517
Fragments arranged by size:
2517 1056 1046
BclI T'GATC_A
Cuts at: 2139 2139
Size: 4619
BglI GCCn_nnn'nGGC
Cuts at: 398 955 398
Size: 557 4062
BglII A'GATC_T
Cuts at: 2134 4171 2134
Size: 2037 2582
BlpI GC'TnA_GC
Cuts at: 1731 1731
Size: 4619
Bme1580I G_kGCm'C
Cuts at: 1156 1226 2244 2337 3461 1156
Size: 70 1018 93 1124 2314
Fragments arranged by size:
2314 1124 1018 93 70
BmrI ACTGGGnnnn_n'
Cuts at: 1831 1912 2247 4391 1831
Size: 81 335 2144 2059
Fragments arranged by size:
2144 2059 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 4619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 4619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 2674 3388 3686 1795
Size: 879 714 298 2728
Fragments arranged by size:
2728 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4323 4323
Size: 4619
BsaAI yAC'GTr
Cuts at: 1972 1972
Size: 4619
BsaBI GATnn'nnATC
Cuts at: 6 569 2133 4170 6
Size: 563 1564 2037 455
Fragments arranged by size:
2037 1564 563 455
BsaHI Gr'CG_yC
Cuts at: 2050 2298 3000 4496 2050
Size: 248 702 1496 2173
Fragments arranged by size:
2173 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1808 2329 3007 3418 3565 3839 1808
Size: 521 678 411 147 274 2588
Fragments arranged by size:
2588 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1598 1628 1598
Size: 30 4589
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 757
Size: 4619
BseYI C'CCAG_C
Cuts at: 1234 1276 2044 2802 3467 3875 1234
Size: 42 768 758 665 408 1978
Fragments arranged by size:
1978 768 758 665 408 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 149
Size: 4619
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3437 104
Size: 2057 46 1230 1286
Fragments arranged by size:
2057 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 964 1046 1156 1633 2411 2601 3461 964
Size: 82 110 477 778 190 860 2122
Fragments arranged by size:
2122 860 778 477 190 110 82
BsiWI C'GTAC_G
Cuts at: 1322 1322
Size: 4619
BsmBI CGTCTCn'nnnn_
Cuts at: 1291 1291
Size: 4619
Bsp1286I G_dGCh'C
Cuts at: 964 1046 1156 1226 1633 2244 2337 2411 2601
Size: 82 110 70 407 611 93 74 190
Cuts at: 2601 2663 3461 964
Size: 62 798 2122
Fragments arranged by size:
2122 798 611 407 190 110 93 82
74 70 62
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 888 1669 1883 2185 2566 237
Size: 651 781 214 302 381 2290
Fragments arranged by size:
2290 781 651 381 302 214
BsrBI CCG'CTC
Cuts at: 50 336 1284 2911 2965 4449 4614 50
Size: 286 948 1627 54 1484 165 55
Fragments arranged by size:
1627 1484 948 286 165 55 54
BsrDI GCAATG_nn'
Cuts at: 996 2531 996
Size: 1535 3084
BsrFI r'CCGG_y
Cuts at: 2617 2798 2617
Size: 181 4438
BssSI C'ACGA_G
Cuts at: 2890 3598 2890
Size: 708 3911
BstAPI GCAn_nnn'nTGC
Cuts at: 980 1996 980
Size: 1016 3603
BstBI TT'CG_AA
Cuts at: 2980 2980
Size: 4619
BstEII G'GTnAC_C
Cuts at: 1191 1191
Size: 4619
BstYI r'GATC_y
Cuts at: 1 1644 2111 2134 2469 2715 3021 3033 3119
Size: 1643 467 23 335 246 306 12 86
Cuts at: 3119 4171 1
Size: 1052 449
Fragments arranged by size:
1643 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1187 107
Size: 1080 3539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2569 2750 2913 2569
Size: 181 163 4275
Fragments arranged by size:
4275 181 163
BtsI GCAGTG_nn'
Cuts at: 1038 1294 1862 1038
Size: 256 568 3795
Fragments arranged by size:
3795 568 256
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 2325 3669 2325
Size: 1344 3275
EaeI y'GGCC_r
Cuts at: 2204 2378 2769 2796 4141 2204
Size: 174 391 27 1345 2682
Fragments arranged by size:
2682 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 302 635 794 1433 2642 2852 302
Size: 333 159 639 1209 210 2069
Fragments arranged by size:
2069 1209 639 333 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 577 2827 3569 3715 4429 577
Size: 2250 742 146 714 767
Fragments arranged by size:
2250 767 742 714 146
EcoICRI GAG'CTC
Cuts at: 1044 1631 1044
Size: 587 4032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 634 1474 2444 2876 3229 3891 326
Size: 308 840 970 432 353 662 1054
Fragments arranged by size:
1054 970 840 662 432 353 308
EcoO109I rG'GnC_Cy
Cuts at: 747 1758 747
Size: 1011 3608
EcoRI G'AATT_C
Cuts at: 378 1005 1623 378
Size: 627 618 3374
Fragments arranged by size:
3374 627 618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4619
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 2053 2301 3531 4302 2053
Size: 248 1230 771 2370
Fragments arranged by size:
2370 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1150 1182 2461 2491 2493 2523 1150
Size: 32 1279 30 2 30 3246
Fragments arranged by size:
3246 1279 32 30 30 2
HincII GTy'rAC
Cuts at: 1308 1658 1308
Size: 350 4269
KasI G'GCGC_C
Cuts at: 2049 2297 2049
Size: 248 4371
KpnI G_GTAC'C
Cuts at: 1639 1639
Size: 4619
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3378 3562 3378
Size: 184 4435
MscI TGG'CCA
Cuts at: 2380 2380
Size: 4619
MslI CAynn'nnrTG
Cuts at: 1099 1562 2608 2735 1099
Size: 463 1046 127 2983
Fragments arranged by size:
2983 1046 463 127
MspA1I CmG'CkG
Cuts at: 1238 1730 2044 2404 3188 3433 1238
Size: 492 314 360 784 245 2424
Fragments arranged by size:
2424 784 492 360 314 245
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 2050 2298 2050
Size: 248 4371
NcoI C'CATG_G
Cuts at: 107 107
Size: 4619
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NspI r_CATG'y
Cuts at: 1233 1672 2172 3775 1233
Size: 439 500 1603 2077
Fragments arranged by size:
2077 1603 500 439
PasI CC'CwG_GG
Cuts at: 710 710
Size: 4619
PciI A'CATG_T
Cuts at: 3771 3771
Size: 4619
PflMI CCAn_nnn'nTGG
Cuts at: 299 1180 299
Size: 881 3738
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 4587
PpuMI rG'GwC_Cy
Cuts at: 747 747
Size: 4619
PshAI GACnn'nnGTC
Cuts at: 1303 1303
Size: 4619
PsiI TTA'TAA
Cuts at: 811 811
Size: 4619
PstI C_TGCA'G
Cuts at: 885 1265 1666 2351 885
Size: 380 401 685 3153
Fragments arranged by size:
3153 685 401 380
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 1238 2044 2404 1238
Size: 806 360 3453
Fragments arranged by size:
3453 806 360
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 1046 1633 1046
Size: 587 4032
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 635 2642 2852 635
Size: 2007 210 2402
Fragments arranged by size:
2402 2007 210
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 4136 4136
Size: 4619
SfcI C'TryA_G
Cuts at: 33 881 1261 1662 2347 3315 3506 33
Size: 848 380 401 685 968 191 1146
Fragments arranged by size:
1146 968 848 685 401 380 191
SfoI GGC'GCC
Cuts at: 2051 2299 2051
Size: 248 4371
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1641 1641
Size: 4619
SmlI C'TyrA_G
Cuts at: 1774 2689 3403 3665 1774
Size: 915 714 262 2728
Fragments arranged by size:
2728 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1672 2172 1672
Size: 500 4119
SspI AAT'ATT
Cuts at: 654 1365 654
Size: 711 3908
StuI AGG'CCT
Cuts at: 1249 1249
Size: 4619
StyI C'CwwG_G
Cuts at: 107 758 1753 2107 107
Size: 651 995 354 2619
Fragments arranged by size:
2619 995 651 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 115 1039 2202 2335 2794 3003 115
Size: 924 1163 133 459 209 1731
Fragments arranged by size:
1731 1163 924 459 209 133
TatI w'GTAC_w
Cuts at: 261 433 4134 261
Size: 172 3701 746
Fragments arranged by size:
3701 746 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 331 363 4136 4168 331
Size: 32 3773 32 782
Fragments arranged by size:
3773 782 32 32
Tth111I GACn'n_nGTC
Cuts at: 2416 2416
Size: 4619
XbaI T'CTAG_A
Cuts at: 1650 1650
Size: 4619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2114
Size: 4619
XmaI C'CCGG_G
Cuts at: 1639 1639
Size: 4619
XmnI GAAnn'nnTTC
Cuts at: 1417 1417
Size: 4619
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsmI BspEI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI