Restriction map for
pF1KB8689
(Circular) (Six-base) MAPSORT of: pF1KB8689.seq Check: 8993 from: 1 to: 4463
pF1KB8689 4463 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 391 391
Size: 4463
AccI GT'mk_AC
Cuts at: 1501 1501
Size: 4463
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 2288 2720 3073 3735 904
Size: 1384 432 353 662 1632
Fragments arranged by size:
1632 1384 662 432 353
AfeI AGC'GCT
Cuts at: 520 520
Size: 4463
AflIII A'CryG_T
Cuts at: 1295 1815 3615 3896 1295
Size: 520 1800 281 1862
Fragments arranged by size:
1862 1800 520 281
AgeI A'CCGG_T
Cuts at: 1117 1117
Size: 4463
AleI CACnn'nnGTG
Cuts at: 715 949 715
Size: 234 4229
AlwNI CAG_nnn'CTG
Cuts at: 1768 3206 1768
Size: 1438 3025
ApaLI G'TGCA_C
Cuts at: 360 3301 360
Size: 2941 1522
ApoI r'AATT_y
Cuts at: 1467 3816 4072 1467
Size: 2349 256 1858
Fragments arranged by size:
2349 1858 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 325 532 1162 1358 1483 325
Size: 207 630 196 125 3305
Fragments arranged by size:
3305 630 207 196 125
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 661 940 1114 1120 1417 1479 1893 2141 2176
Size: 279 174 6 297 62 414 248 35
Cuts at: 2176 661
Size: 2948
Fragments arranged by size:
2948 414 297 279 248 174 62 35
6
BanII G_rGCy'C
Cuts at: 824 1477 2507 824
Size: 653 1030 2780
Fragments arranged by size:
2780 1030 653
BbeI G_GCGC'C
Cuts at: 665 1421 1897 2145 665
Size: 756 476 248 2983
Fragments arranged by size:
2983 756 476 248
BbsI GAAGACnn'nnnn_
Cuts at: 1236 1236
Size: 4463
BbvCI CC'TCA_GC
Cuts at: 1109 1109
Size: 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 316 350 930 964 90
Size: 34 192 34 580 34 3589
Fragments arranged by size:
3589 580 192 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3417 4463 2361
Size: 1056 1046 2361
Fragments arranged by size:
2361 1056 1046
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BglI GCCn_nnn'nGGC
Cuts at: 965 1058 965
Size: 93 4370
BglII A'GATC_T
Cuts at: 536 1978 4015 536
Size: 1442 2037 984
Fragments arranged by size:
2037 1442 984
BlpI GC'TnA_GC
Cuts at: 729 1575 729
Size: 846 3617
Bme1580I G_kGCm'C
Cuts at: 364 1117 1125 2088 2181 3305 364
Size: 753 8 963 93 1124 1522
Fragments arranged by size:
1522 1124 963 753 93 8
BmgBI CAC'GTC
Cuts at: 290 1269 290
Size: 979 3484
BmrI ACTGGGnnnn_n'
Cuts at: 1035 1675 1756 2091 4235 1035
Size: 640 81 335 2144 1263
Fragments arranged by size:
2144 1263 640 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 300
Size: 4463
Bpu10I CC'TnA_GC
Cuts at: 1109 1109
Size: 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1639 2518 3232 3530 244
Size: 1395 879 714 298 1177
Fragments arranged by size:
1395 1177 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1045 1159 4167 1045
Size: 114 3008 1341
Fragments arranged by size:
3008 1341 114
BsaAI yAC'GTr
Cuts at: 915 1816 915
Size: 901 3562
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 662 1418 1894 2142 2844 4340 662
Size: 756 476 248 702 1496 785
Fragments arranged by size:
1496 785 756 702 476 248
BsaWI w'CCGG_w
Cuts at: 1117 1652 2173 2851 3262 3409 3683 1117
Size: 535 521 678 411 147 274 1897
Fragments arranged by size:
1897 678 535 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 436 466 1210 1240 436
Size: 30 744 30 3659
Fragments arranged by size:
3659 744 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1243 1243
Size: 4463
BseYI C'CCAG_C
Cuts at: 306 1888 2646 3311 3719 306
Size: 1582 758 665 408 1050
Fragments arranged by size:
1582 1050 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 676 718 1133 1324 356
Size: 320 42 415 191 3495
Fragments arranged by size:
3495 415 320 191 42
BsiEI CG_ry'CG
Cuts at: 104 413 503 636 830 2005 2051 3281 104
Size: 309 90 133 194 1175 46 1230 1286
Fragments arranged by size:
1286 1230 1175 309 194 133 90 46
BsiHKAI G_wGCw'C
Cuts at: 364 824 1477 2255 2445 3305 364
Size: 460 653 778 190 860 1522
Fragments arranged by size:
1522 860 778 653 460 190
Bsp1286I G_dGCh'C
Cuts at: 364 824 1117 1125 1477 2088 2181 2255 2445
Size: 460 293 8 352 611 93 74 190
Cuts at: 2445 2507 3305 364
Size: 62 798 1522
Fragments arranged by size:
1522 798 611 460 352 293 190 93
74 62 8
BspHI T'CATG_A
Cuts at: 434 2895 434
Size: 2461 2002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 391 655 690 1373 1513 1727 2029 2410 391
Size: 264 35 683 140 214 302 381 2444
Fragments arranged by size:
2444 683 381 302 264 214 140 35
BsrBI CCG'CTC
Cuts at: 50 672 1431 2755 2809 4293 4458 50
Size: 622 759 1324 54 1484 165 55
Fragments arranged by size:
1484 1324 759 622 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2375 2375
Size: 4463
BsrFI r'CCGG_y
Cuts at: 498 1117 1212 2461 2642 498
Size: 619 95 1249 181 2319
Fragments arranged by size:
2319 1249 619 181 95
BssSI C'ACGA_G
Cuts at: 2734 3442 2734
Size: 708 3755
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstEII G'GTnAC_C
Cuts at: 391 702 391
Size: 311 4152
BstXI CCAn_nnnn'nTGG
Cuts at: 951 951
Size: 4463
BstYI r'GATC_y
Cuts at: 1 254 536 920 1488 1955 1978 2313 2559
Size: 253 282 384 568 467 23 335 246
Cuts at: 2559 2865 2877 2963 4015 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 568 467 449 384 335 306 282
253 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 619 619
Size: 4463
BtgI C'CryG_G
Cuts at: 417 1187 1259 417
Size: 770 72 3621
Fragments arranged by size:
3621 770 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2413 2594 2757 2413
Size: 181 163 4119
Fragments arranged by size:
4119 181 163
BtsI GCAGTG_nn'
Cuts at: 737 1706 737
Size: 969 3494
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3513 2169
Size: 1344 3119
EaeI y'GGCC_r
Cuts at: 199 410 500 572 667 734 827 1190 1283
Size: 211 90 72 95 67 93 363 93
Cuts at: 1283 2048 2222 2613 2640 3985 199
Size: 765 174 391 27 1345 677
Fragments arranged by size:
1345 765 677 391 363 211 174 95
93 93 90 72 67 27
EagI C'GGCC_G
Cuts at: 410 500 827 2048 410
Size: 90 327 1221 2825
Fragments arranged by size:
2825 1221 327 90
EarI CTCTTCn'nnn_
Cuts at: 547 607 2486 2696 547
Size: 60 1879 210 2314
Fragments arranged by size:
2314 1879 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 384 2671 3413 3559 4273 384
Size: 2287 742 146 714 574
Fragments arranged by size:
2287 742 714 574 146
EcoICRI GAG'CTC
Cuts at: 822 1475 822
Size: 653 3810
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 904 2288 2720 3073 3735 300
Size: 604 1384 432 353 662 1028
Fragments arranged by size:
1384 1028 662 604 432 353
EcoO109I rG'GnC_Cy
Cuts at: 429 609 1221 1362 1602 429
Size: 180 612 141 240 3290
Fragments arranged by size:
3290 612 240 180 141
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4463
FspI TGC'GCA
Cuts at: 346 487 2244 346
Size: 141 1757 2565
Fragments arranged by size:
2565 1757 141
HaeII r_GCGC'y
Cuts at: 287 522 665 1421 1897 2145 3375 4146 287
Size: 235 143 756 476 248 1230 771 604
Fragments arranged by size:
1230 771 756 604 476 248 235 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 886 918 2305 2335 2337 2367 886
Size: 32 1387 30 2 30 2982
Fragments arranged by size:
2982 1387 32 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
KasI G'GCGC_C
Cuts at: 661 1417 1893 2141 661
Size: 756 476 248 2983
Fragments arranged by size:
2983 756 476 248
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 1295 3896 1295
Size: 2601 1862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1280 1349 3222 3406 1280
Size: 69 1873 184 2337
Fragments arranged by size:
2337 1873 184 69
MscI TGG'CCA
Cuts at: 1192 2224 1192
Size: 1032 3431
MslI CAynn'nnrTG
Cuts at: 715 949 952 1103 1346 2452 2579 715
Size: 234 3 151 243 1106 127 2599
Fragments arranged by size:
2599 1106 243 234 151 127 3
MspA1I CmG'CkG
Cuts at: 733 1574 1888 2248 3032 3277 733
Size: 841 314 360 784 245 1919
Fragments arranged by size:
1919 841 784 360 314 245
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 4463
NarI GG'CG_CC
Cuts at: 662 1418 1894 2142 662
Size: 756 476 248 2983
Fragments arranged by size:
2983 756 476 248
NcoI C'CATG_G
Cuts at: 417 417
Size: 4463
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 4463
NspI r_CATG'y
Cuts at: 996 1516 2016 3619 996
Size: 520 500 1603 1840
Fragments arranged by size:
1840 1603 520 500
PasI CC'CwG_GG
Cuts at: 189 189
Size: 4463
PciI A'CATG_T
Cuts at: 3615 3615
Size: 4463
PflMI CCAn_nnn'nTGG
Cuts at: 915 915
Size: 4463
PfoI T'CCnGG_A
Cuts at: 776 776
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PmlI CAC'GTG
Cuts at: 915 915
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 4431
PpuMI rG'GwC_Cy
Cuts at: 429 609 1221 1362 429
Size: 180 612 141 3530
Fragments arranged by size:
3530 612 180 141
PstI C_TGCA'G
Cuts at: 226 1510 2195 226
Size: 1284 685 2494
Fragments arranged by size:
2494 1284 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 1888 2248 1888
Size: 360 4103
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 824 1477 824
Size: 653 3810
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SanDI GG'GwC_CC
Cuts at: 609 609
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 547 2486 2696 547
Size: 1939 210 2314
Fragments arranged by size:
2314 1939 210
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SfcI C'TryA_G
Cuts at: 33 222 1506 2191 3159 3350 33
Size: 189 1284 685 968 191 1146
Fragments arranged by size:
1284 1146 968 685 191 189
SfoI GGC'GCC
Cuts at: 663 1419 1895 2143 663
Size: 756 476 248 2983
Fragments arranged by size:
2983 756 476 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SgrAI Cr'CCGG_yG
Cuts at: 1117 1117
Size: 4463
SmaI CCC'GGG
Cuts at: 1485 1485
Size: 4463
SmlI C'TyrA_G
Cuts at: 259 1618 2533 3247 3509 259
Size: 1359 915 714 262 1213
Fragments arranged by size:
1359 1213 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 996 1516 2016 996
Size: 520 500 3443
Fragments arranged by size:
3443 520 500
StuI AGG'CCT
Cuts at: 1093 1237 1093
Size: 144 4319
StyI C'CwwG_G
Cuts at: 118 417 455 1597 1951 118
Size: 299 38 1142 354 2630
Fragments arranged by size:
2630 1142 354 299 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1167 2046 2179 2638 2847 1167
Size: 879 133 459 209 2783
Fragments arranged by size:
2783 879 459 209 133
TatI w'GTAC_w
Cuts at: 932 1226 3978 932
Size: 294 2752 1417
Fragments arranged by size:
2752 1417 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 436 468 3980 4012 436
Size: 32 3512 32 887
Fragments arranged by size:
3512 887 32 32
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1958 1958
Size: 4463
XmaI C'CCGG_G
Cuts at: 1483 1483
Size: 4463
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI