Restriction map for
pF1KB8690
(Circular) (Six-base) MAPSORT of: pF1KB8690.seq Check: 9159 from: 1 to: 4946
pF1KB8690 4946 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 812 812
Size: 4946
AccI GT'mk_AC
Cuts at: 1984 1984
Size: 4946
Acc65I G'GTAC_C
Cuts at: 1962 1962
Size: 4946
AclI AA'CG_TT
Cuts at: 4786 4786
Size: 4946
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 2771 3203 3556 4218 199
Size: 2572 432 353 662 927
Fragments arranged by size:
2572 927 662 432 353
AflIII A'CryG_T
Cuts at: 2298 4098 4379 2298
Size: 1800 281 2865
Fragments arranged by size:
2865 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1811 1811
Size: 4946
AlwNI CAG_nnn'CTG
Cuts at: 122 449 522 804 2251 3689 122
Size: 327 73 282 1447 1438 1379
Fragments arranged by size:
1447 1438 1379 327 282 73
ApaI G_GGCC'C
Cuts at: 229 1071 229
Size: 842 4104
ApaLI G'TGCA_C
Cuts at: 1562 3784 1562
Size: 2222 2724
ApoI r'AATT_y
Cuts at: 1950 4299 4555 1950
Size: 2349 256 2341
Fragments arranged by size:
2349 2341 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4946
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
AvaI C'yCGr_G
Cuts at: 229 282 556 566 682 783 1164 1336 1349
Size: 53 274 10 116 101 381 172 13
Cuts at: 1349 1581 1966 229
Size: 232 385 3209
Fragments arranged by size:
3209 385 381 274 232 172 116 101
53 13 10
BamHI G'GATC_C
Cuts at: 1971 1971
Size: 4946
BanI G'GyrC_C
Cuts at: 369 708 1084 1176 1599 1962 2376 2624 2659
Size: 339 376 92 423 363 414 248 35
Cuts at: 2659 369
Size: 2656
Fragments arranged by size:
2656 423 414 376 363 339 248 92
35
BanII G_rGCy'C
Cuts at: 229 905 1071 1588 1683 1717 1960 2990 229
Size: 676 166 517 95 34 243 1030 2185
Fragments arranged by size:
2185 1030 676 517 243 166 95 34
BbeI G_GCGC'C
Cuts at: 1180 2380 2628 1180
Size: 1200 248 3498
Fragments arranged by size:
3498 1200 248
BbsI GAAGACnn'nnnn_
Cuts at: 1865 1865
Size: 4946
BbvCI CC'TCA_GC
Cuts at: 1754 1754
Size: 4946
BciVI GTATCCnnnnn_n'
Cuts at: 2844 3900 4946 2844
Size: 1056 1046 2844
Fragments arranged by size:
2844 1056 1046
BclI T'GATC_A
Cuts at: 2466 2466
Size: 4946
BglI GCCn_nnn'nGGC
Cuts at: 224 224
Size: 4946
BglII A'GATC_T
Cuts at: 2461 4498 2461
Size: 2037 2909
BlpI GC'TnA_GC
Cuts at: 1411 1716 2058 1411
Size: 305 342 4299
Fragments arranged by size:
4299 342 305
Bme1580I G_kGCm'C
Cuts at: 229 289 755 1071 1566 1894 2571 2664 3788
Size: 60 466 316 495 328 677 93 1124
Cuts at: 3788 229
Size: 1387
Fragments arranged by size:
1387 1124 677 495 466 328 316 93
60
BmrI ACTGGGnnnn_n'
Cuts at: 2158 2239 2574 4718 2158
Size: 81 335 2144 2386
Fragments arranged by size:
2386 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1150 3313 1150
Size: 2163 2783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 325 346 478 682 952 1438 1555 147
Size: 178 21 132 204 270 486 117 3538
Fragments arranged by size:
3538 486 270 204 178 132 117 21
Bpu10I CC'TnA_GC
Cuts at: 1754 1754
Size: 4946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 3001 3715 4013 2122
Size: 879 714 298 3055
Fragments arranged by size:
3055 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4650 4650
Size: 4946
BsaAI yAC'GTr
Cuts at: 2299 2299
Size: 4946
BsaBI GATnn'nnATC
Cuts at: 6 2460 4497 6
Size: 2454 2037 455
Fragments arranged by size:
2454 2037 455
BsaHI Gr'CG_yC
Cuts at: 251 745 1177 1748 2377 2625 3327 4823 251
Size: 494 432 571 629 248 702 1496 374
Fragments arranged by size:
1496 702 629 571 494 432 374 248
BsaWI w'CCGG_w
Cuts at: 1257 2135 2656 3334 3745 3892 4166 1257
Size: 878 521 678 411 147 274 2037
Fragments arranged by size:
2037 878 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1615 1645 1651 1681 1615
Size: 30 6 30 4880
Fragments arranged by size:
4880 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 670 886 949 1128 1233 1552 1629 1755 1842
Size: 216 63 179 105 319 77 126 87
Cuts at: 1842 670
Size: 3774
Fragments arranged by size:
3774 319 216 179 126 105 87 77
63
BseYI C'CCAG_C
Cuts at: 625 1157 1247 1397 1895 2371 3129 3794 4202
Size: 532 90 150 498 476 758 665 408
Cuts at: 4202 625
Size: 1369
Fragments arranged by size:
1369 758 665 532 498 476 408 150
90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 1387 1951 1366
Size: 21 564 4361
Fragments arranged by size:
4361 564 21
BsiEI CG_ry'CG
Cuts at: 104 215 2488 2534 3764 104
Size: 111 2273 46 1230 1286
Fragments arranged by size:
2273 1286 1230 111 46
BsiHKAI G_wGCw'C
Cuts at: 575 620 905 1566 1960 2738 2928 3788 575
Size: 45 285 661 394 778 190 860 1733
Fragments arranged by size:
1733 860 778 661 394 285 190 45
BsmBI CGTCTCn'nnnn_
Cuts at: 1305 1305
Size: 4946
Bsp1286I G_dGCh'C
Cuts at: 229 289 575 620 755 905 1071 1566 1588
Size: 60 286 45 135 150 166 495 22
Cuts at: 1588 1683 1717 1894 1960 2571 2664 2738 2928
Size: 95 34 177 66 611 93 74 190
Cuts at: 2928 2990 3788 229
Size: 62 798 1387
Fragments arranged by size:
1387 798 611 495 286 190 177 166
150 135 95 93 74 66 62 60
45 34 22
BspHI T'CATG_A
Cuts at: 3378 3378
Size: 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 812 1098 1996 2210 2512 2893 812
Size: 286 898 214 302 381 2865
Fragments arranged by size:
2865 898 381 302 286 214
BsrBI CCG'CTC
Cuts at: 50 555 956 1699 3238 3292 4776 4941 50
Size: 505 401 743 1539 54 1484 165 55
Fragments arranged by size:
1539 1484 743 505 401 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2858 2858
Size: 4946
BsrFI r'CCGG_y
Cuts at: 214 827 1328 2944 3125 214
Size: 613 501 1616 181 2035
Fragments arranged by size:
2035 1616 613 501 181
BssHII G'CGCG_C
Cuts at: 200 200
Size: 4946
BssSI C'ACGA_G
Cuts at: 3217 3925 3217
Size: 708 4238
BstAPI GCAn_nnn'nTGC
Cuts at: 804 2323 804
Size: 1519 3427
BstBI TT'CG_AA
Cuts at: 3307 3307
Size: 4946
BstEII G'GTnAC_C
Cuts at: 1331 1331
Size: 4946
BstXI CCAn_nnnn'nTGG
Cuts at: 996 996
Size: 4946
BstYI r'GATC_y
Cuts at: 1 950 1971 2438 2461 2796 3042 3348 3360
Size: 949 1021 467 23 335 246 306 12
Cuts at: 3360 3446 4498 1
Size: 86 1052 449
Fragments arranged by size:
1052 1021 949 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 131 1140 1910 131
Size: 1009 770 3167
Fragments arranged by size:
3167 1009 770
BtgI C'CryG_G
Cuts at: 107 492 691 1232 1362 107
Size: 385 199 541 130 3691
Fragments arranged by size:
3691 541 385 199 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2896 3077 3240 2896
Size: 181 163 4602
Fragments arranged by size:
4602 181 163
BtsI GCAGTG_nn'
Cuts at: 595 774 826 1125 1323 1378 1939 2189 595
Size: 179 52 299 198 55 561 250 3352
Fragments arranged by size:
3352 561 299 250 198 179 55 52
DraI TTT'AAA
Cuts at: 1945 1945
Size: 4946
DraIII CAC_nnn'GTG
Cuts at: 913 913
Size: 4946
DrdI GACnn_nn'nnGTC
Cuts at: 2652 3996 2652
Size: 1344 3602
EaeI y'GGCC_r
Cuts at: 212 857 1122 1290 2531 2705 3096 3123 4468
Size: 645 265 168 1241 174 391 27 1345
Cuts at: 4468 212
Size: 690
Fragments arranged by size:
1345 1241 690 645 391 265 174 168
27
EagI C'GGCC_G
Cuts at: 212 2531 212
Size: 2319 2627
EarI CTCTTCn'nnn_
Cuts at: 274 2969 3179 274
Size: 2695 210 2041
Fragments arranged by size:
2695 2041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 378 1160 3154 3896 4042 4756 378
Size: 782 1994 742 146 714 568
Fragments arranged by size:
1994 782 742 714 568 146
EcoICRI GAG'CTC
Cuts at: 903 1958 903
Size: 1055 3891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 199 325 346 478 682 952 1438 1555
Size: 52 126 21 132 204 270 486 117
Cuts at: 1555 2771 3203 3556 4218 147
Size: 1216 432 353 662 875
Fragments arranged by size:
1216 875 662 486 432 353 270 204
132 126 117 52 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1770 1770
Size: 4946
EcoO109I rG'GnC_Cy
Cuts at: 135 226 563 973 1067 1068 1530 1836 1906
Size: 91 337 410 94 1 462 306 70
Cuts at: 1906 2085 135
Size: 179 2996
Fragments arranged by size:
2996 462 410 337 306 179 94 91
70 1
EcoRI G'AATT_C
Cuts at: 1950 1950
Size: 4946
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4946
FseI GG_CCGG'CC
Cuts at: 218 218
Size: 4946
FspI TGC'GCA
Cuts at: 2727 2727
Size: 4946
HaeII r_GCGC'y
Cuts at: 699 723 1180 2380 2628 3858 4629 699
Size: 24 457 1200 248 1230 771 1016
Fragments arranged by size:
1230 1200 1016 771 457 248 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1615 1647 1797 1801 1829 1833 1855 1887 2788
Size: 32 150 4 28 4 22 32 901
Cuts at: 2788 2818 2820 2850 1615
Size: 30 2 30 3711
Fragments arranged by size:
3711 901 150 32 32 30 30 28
22 4 4 2
HincII GTy'rAC
Cuts at: 1985 1985
Size: 4946
KasI G'GCGC_C
Cuts at: 1176 2376 2624 1176
Size: 1200 248 3498
Fragments arranged by size:
3498 1200 248
KpnI G_GTAC'C
Cuts at: 1966 1966
Size: 4946
MluI A'CGCG_T
Cuts at: 4379 4379
Size: 4946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3705 3889 3705
Size: 184 4762
MscI TGG'CCA
Cuts at: 859 2707 859
Size: 1848 3098
MslI CAynn'nnrTG
Cuts at: 994 2935 3062 994
Size: 1941 127 2878
Fragments arranged by size:
2878 1941 127
MspA1I CmG'CkG
Cuts at: 531 647 693 1077 1157 1364 1401 1651 2057
Size: 116 46 384 80 207 37 250 406
Cuts at: 2057 2371 2731 3515 3760 531
Size: 314 360 784 245 1717
Fragments arranged by size:
1717 784 406 384 360 314 250 245
207 116 80 46 37
NaeI GCC'GGC
Cuts at: 216 3127 216
Size: 2911 2035
NarI GG'CG_CC
Cuts at: 1177 2377 2625 1177
Size: 1200 248 3498
Fragments arranged by size:
3498 1200 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4946
NgoMIV G'CCGG_C
Cuts at: 214 3125 214
Size: 2911 2035
NspI r_CATG'y
Cuts at: 1999 2499 4102 1999
Size: 500 1603 2843
Fragments arranged by size:
2843 1603 500
PasI CC'CwG_GG
Cuts at: 1099 1099
Size: 4946
PciI A'CATG_T
Cuts at: 4098 4098
Size: 4946
PfoI T'CCnGG_A
Cuts at: 296 296
Size: 4946
PmeI GTTT'AAAC
Cuts at: 1945 1945
Size: 4946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4914
PpuMI rG'GwC_Cy
Cuts at: 563 1836 563
Size: 1273 3673
PshAI GACnn'nnGTC
Cuts at: 1022 1022
Size: 4946
PspOMI G'GGCC_C
Cuts at: 225 1067 225
Size: 842 4104
PspXI vC'TCGA_Gb
Cuts at: 556 783 1581 556
Size: 227 798 3921
Fragments arranged by size:
3921 798 227
PstI C_TGCA'G
Cuts at: 800 1688 1993 2678 800
Size: 888 305 685 3068
Fragments arranged by size:
3068 888 685 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4946
PvuII CAG'CTG
Cuts at: 1157 1401 2371 2731 1157
Size: 244 970 360 3372
Fragments arranged by size:
3372 970 360 244
RsrII CG'GwC_CG
Cuts at: 3141 3141
Size: 4946
SacI G_AGCT'C
Cuts at: 905 1960 905
Size: 1055 3891
SacII CC_GC'GG
Cuts at: 694 1365 694
Size: 671 4275
SalI G'TCGA_C
Cuts at: 1983 1983
Size: 4946
SanDI GG'GwC_CC
Cuts at: 563 1836 563
Size: 1273 3673
SapI GCTCTTCn'nnn_
Cuts at: 274 2969 3179 274
Size: 2695 210 2041
Fragments arranged by size:
2695 2041 210
SbfI CC_TGCA'GG
Cuts at: 1993 1993
Size: 4946
ScaI AGT'ACT
Cuts at: 4463 4463
Size: 4946
SfcI C'TryA_G
Cuts at: 33 796 1684 1989 2674 3642 3833 33
Size: 763 888 305 685 968 191 1146
Fragments arranged by size:
1146 968 888 763 685 305 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 224 224
Size: 4946
SfoI GGC'GCC
Cuts at: 1178 2378 2626 1178
Size: 1200 248 3498
Fragments arranged by size:
3498 1200 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
SmaI CCC'GGG
Cuts at: 284 1338 1351 1968 284
Size: 1054 13 617 3262
Fragments arranged by size:
3262 1054 617 13
SmlI C'TyrA_G
Cuts at: 556 783 1581 2101 3016 3730 3992 556
Size: 227 798 520 915 714 262 1510
Fragments arranged by size:
1510 915 798 714 520 262 227
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4946
SphI G_CATG'C
Cuts at: 1999 2499 1999
Size: 500 4446
SrfI GCCC'GGGC
Cuts at: 1351 1351
Size: 4946
StyI C'CwwG_G
Cuts at: 107 407 854 1721 2080 2434 107
Size: 300 447 867 359 354 2619
Fragments arranged by size:
2619 867 447 359 354 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1069 1318 1805 2529 2662 3121 3330 1069
Size: 249 487 724 133 459 209 2685
Fragments arranged by size:
2685 724 487 459 249 209 133
TatI w'GTAC_w
Cuts at: 479 4461 479
Size: 3982 964
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 380 412 1153 1185 4463 4495 380
Size: 32 741 32 3278 32 831
Fragments arranged by size:
3278 831 741 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 744 1869 2743 744
Size: 1125 874 2947
Fragments arranged by size:
2947 1125 874
XbaI T'CTAG_A
Cuts at: 1977 1977
Size: 4946
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2441 2441
Size: 4946
XhoI C'TCGA_G
Cuts at: 556 783 1581 556
Size: 227 798 3921
Fragments arranged by size:
3921 798 227
XmaI C'CCGG_G
Cuts at: 282 1336 1349 1966 282
Size: 1054 13 617 3262
Fragments arranged by size:
3262 1054 617 13
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BstZ17I ClaI CspCI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PsiI PsrI SexAI SgrAI SnaBI SspI
StuI SwaI XmnI ZraI