Restriction map for
pF1KB8691
(Circular) (Six-base) MAPSORT of: pF1KB8691.seq Check: 865 from: 1 to: 5192
pF1KB8691 5192 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2230 2230
Size: 5192
Acc65I G'GTAC_C
Cuts at: 2208 2208
Size: 5192
AclI AA'CG_TT
Cuts at: 5032 5032
Size: 5192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2122 3017 3449 3802 4464 1682
Size: 440 895 432 353 662 2410
Fragments arranged by size:
2410 895 662 440 432 353
AflIII A'CryG_T
Cuts at: 2544 4344 4625 2544
Size: 1800 281 3111
Fragments arranged by size:
3111 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 279 762 1234 2497 3935 279
Size: 483 472 1263 1438 1536
Fragments arranged by size:
1536 1438 1263 483 472
ApaI G_GGCC'C
Cuts at: 310 543 310
Size: 233 4959
ApaLI G'TGCA_C
Cuts at: 4030 4030
Size: 5192
ApoI r'AATT_y
Cuts at: 932 2196 4545 4801 932
Size: 1264 2349 256 1323
Fragments arranged by size:
2349 1323 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5192
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5192
AvaI C'yCGr_G
Cuts at: 243 343 477 2212 243
Size: 100 134 1735 3223
Fragments arranged by size:
3223 1735 134 100
BamHI G'GATC_C
Cuts at: 356 2217 356
Size: 1861 3331
BanI G'GyrC_C
Cuts at: 429 570 1139 2208 2622 2870 2905 429
Size: 141 569 1069 414 248 35 2716
Fragments arranged by size:
2716 1069 569 414 248 141 35
BanII G_rGCy'C
Cuts at: 310 543 1309 2206 3236 310
Size: 233 766 897 1030 2266
Fragments arranged by size:
2266 1030 897 766 233
BbeI G_GCGC'C
Cuts at: 433 574 2626 2874 433
Size: 141 2052 248 2751
Fragments arranged by size:
2751 2052 248 141
BbsI GAAGACnn'nnnn_
Cuts at: 858 968 1388 1415 1670 1946 858
Size: 110 420 27 255 276 4104
Fragments arranged by size:
4104 420 276 255 110 27
BciVI GTATCCnnnnn_n'
Cuts at: 3090 4146 5192 3090
Size: 1056 1046 3090
Fragments arranged by size:
3090 1056 1046
BclI T'GATC_A
Cuts at: 944 1373 2712 944
Size: 429 1339 3424
Fragments arranged by size:
3424 1339 429
BglII A'GATC_T
Cuts at: 1424 1460 2707 4744 1424
Size: 36 1247 2037 1872
Fragments arranged by size:
2037 1872 1247 36
BlpI GC'TnA_GC
Cuts at: 2304 2304
Size: 5192
Bme1580I G_kGCm'C
Cuts at: 310 543 2817 2910 4034 310
Size: 233 2274 93 1124 1468
Fragments arranged by size:
2274 1468 1124 233 93
BmgBI CAC'GTC
Cuts at: 955 1974 955
Size: 1019 4173
BmrI ACTGGGnnnn_n'
Cuts at: 313 2404 2485 2820 4964 313
Size: 2091 81 335 2144 541
Fragments arranged by size:
2144 2091 541 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2004 3559 2004
Size: 1555 3637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 784 805 883 1293 235
Size: 549 21 78 410 4134
Fragments arranged by size:
4134 549 410 78 21
Bpu10I CC'TnA_GC
Cuts at: 151 1101 151
Size: 950 4242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2368 3247 3961 4259 811
Size: 1557 879 714 298 1744
Fragments arranged by size:
1744 1557 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 866 1471 1489 1573 4896 866
Size: 605 18 84 3323 1162
Fragments arranged by size:
3323 1162 605 84 18
BsaAI yAC'GTr
Cuts at: 2545 2545
Size: 5192
BsaBI GATnn'nnATC
Cuts at: 6 1981 2706 4743 6
Size: 1975 725 2037 455
Fragments arranged by size:
2037 1975 725 455
BsaHI Gr'CG_yC
Cuts at: 430 571 1385 2623 2871 3573 5069 430
Size: 141 814 1238 248 702 1496 553
Fragments arranged by size:
1496 1238 814 702 553 248 141
BsaWI w'CCGG_w
Cuts at: 2182 2381 2902 3580 3991 4138 4412 2182
Size: 199 521 678 411 147 274 2962
Fragments arranged by size:
2962 678 521 411 274 199 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 361 672 702 2083 199
Size: 162 311 30 1381 3308
Fragments arranged by size:
3308 1381 311 162 30
BseYI C'CCAG_C
Cuts at: 233 395 2617 3375 4040 4448 233
Size: 162 2222 758 665 408 977
Fragments arranged by size:
2222 977 758 665 408 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1658 1658
Size: 5192
BsiEI CG_ry'CG
Cuts at: 104 2734 2780 4010 104
Size: 2630 46 1230 1286
Fragments arranged by size:
2630 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2206 2984 3174 4034 2206
Size: 778 190 860 3364
Fragments arranged by size:
3364 860 778 190
BsmI GAATG_Cn'
Cuts at: 913 913
Size: 5192
BsmBI CGTCTCn'nnnn_
Cuts at: 829 829
Size: 5192
Bsp1286I G_dGCh'C
Cuts at: 310 543 1309 2206 2817 2910 2984 3174 3236
Size: 233 766 897 611 93 74 190 62
Cuts at: 3236 4034 310
Size: 798 1468
Fragments arranged by size:
1468 897 798 766 611 233 190 93
74 62
BspHI T'CATG_A
Cuts at: 1449 3624 1449
Size: 2175 3017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 981 2242 2456 2758 3139 981
Size: 1261 214 302 381 3034
Fragments arranged by size:
3034 1261 381 302 214
BsrBI CCG'CTC
Cuts at: 50 207 645 1100 3484 3538 5022 5187 50
Size: 157 438 455 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 455 438 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 1878 3104 1878
Size: 1226 3966
BsrFI r'CCGG_y
Cuts at: 1106 3190 3371 1106
Size: 2084 181 2927
Fragments arranged by size:
2927 2084 181
BsrGI T'GTAC_A
Cuts at: 510 510
Size: 5192
BssSI C'ACGA_G
Cuts at: 3463 4171 3463
Size: 708 4484
BstAPI GCAn_nnn'nTGC
Cuts at: 2569 2569
Size: 5192
BstBI TT'CG_AA
Cuts at: 3553 3553
Size: 5192
BstXI CCAn_nnnn'nTGG
Cuts at: 902 1136 2153 902
Size: 234 1017 3941
Fragments arranged by size:
3941 1017 234
BstYI r'GATC_y
Cuts at: 1 356 780 999 1424 1460 2217 2684 2707
Size: 355 424 219 425 36 757 467 23
Cuts at: 2707 3042 3288 3594 3606 3692 4744 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 757 467 449 425 424 355 335
306 246 219 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 470 470
Size: 5192
BtgI C'CryG_G
Cuts at: 107 440 1016 1761 107
Size: 333 576 745 3538
Fragments arranged by size:
3538 745 576 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1910 3142 3323 3486 1910
Size: 1232 181 163 3616
Fragments arranged by size:
3616 1232 181 163
BtsI GCAGTG_nn'
Cuts at: 566 797 1409 1863 2435 566
Size: 231 612 454 572 3323
Fragments arranged by size:
3323 612 572 454 231
ClaI AT'CG_AT
Cuts at: 497 497
Size: 5192
DraI TTT'AAA
Cuts at: 2191 2191
Size: 5192
DrdI GACnn_nn'nnGTC
Cuts at: 2898 4242 2898
Size: 1344 3848
EaeI y'GGCC_r
Cuts at: 134 281 2777 2951 3342 3369 4714 134
Size: 147 2496 174 391 27 1345 612
Fragments arranged by size:
2496 1345 612 391 174 147 27
EagI C'GGCC_G
Cuts at: 2777 2777
Size: 5192
EarI CTCTTCn'nnn_
Cuts at: 589 599 703 1688 2003 2164 3215 3425 589
Size: 10 104 985 315 161 1051 210 2356
Fragments arranged by size:
2356 1051 985 315 210 161 104 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 699 3400 4142 4288 5002 699
Size: 2701 742 146 714 889
Fragments arranged by size:
2701 889 742 714 146
EcoICRI GAG'CTC
Cuts at: 2204 2204
Size: 5192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 784 805 883 1293 1682 2122 3017 3449
Size: 549 21 78 410 389 440 895 432
Cuts at: 3449 3802 4464 235
Size: 353 662 963
Fragments arranged by size:
963 895 662 549 440 432 410 389
353 78 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1228 1228
Size: 5192
EcoO109I rG'GnC_Cy
Cuts at: 127 307 539 540 1566 2142 2331 127
Size: 180 232 1 1026 576 189 2988
Fragments arranged by size:
2988 1026 576 232 189 180 1
EcoRI G'AATT_C
Cuts at: 2196 2196
Size: 5192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1056 3211 1056
Size: 2155 3037
FspI TGC'GCA
Cuts at: 2973 2973
Size: 5192
HaeII r_GCGC'y
Cuts at: 243 433 574 2626 2874 4104 4875 243
Size: 190 141 2052 248 1230 771 560
Fragments arranged by size:
2052 1230 771 560 248 190 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 863 895 3034 3064 3066 3096 863
Size: 32 2139 30 2 30 2959
Fragments arranged by size:
2959 2139 32 30 30 2
HincII GTy'rAC
Cuts at: 588 1275 2231 588
Size: 687 956 3549
Fragments arranged by size:
3549 956 687
HpaI GTT'AAC
Cuts at: 1275 1275
Size: 5192
KasI G'GCGC_C
Cuts at: 429 570 2622 2870 429
Size: 141 2052 248 2751
Fragments arranged by size:
2751 2052 248 141
KpnI G_GTAC'C
Cuts at: 2212 2212
Size: 5192
MluI A'CGCG_T
Cuts at: 4625 4625
Size: 5192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 237 3951 4135 237
Size: 3714 184 1294
Fragments arranged by size:
3714 1294 184
MscI TGG'CCA
Cuts at: 2953 2953
Size: 5192
MslI CAynn'nnrTG
Cuts at: 619 844 1066 3181 3308 619
Size: 225 222 2115 127 2503
Fragments arranged by size:
2503 2115 225 222 127
MspA1I CmG'CkG
Cuts at: 222 777 1136 1205 2303 2617 2977 3761 4006
Size: 555 359 69 1098 314 360 784 245
Cuts at: 4006 222
Size: 1408
Fragments arranged by size:
1408 1098 784 555 360 359 314 245
69
NaeI GCC'GGC
Cuts at: 1108 3373 1108
Size: 2265 2927
NarI GG'CG_CC
Cuts at: 430 571 2623 2871 430
Size: 141 2052 248 2751
Fragments arranged by size:
2751 2052 248 141
NcoI C'CATG_G
Cuts at: 107 1016 1761 107
Size: 909 745 3538
Fragments arranged by size:
3538 909 745
NgoMIV G'CCGG_C
Cuts at: 1106 3371 1106
Size: 2265 2927
NspI r_CATG'y
Cuts at: 2245 2745 4348 2245
Size: 500 1603 3089
Fragments arranged by size:
3089 1603 500
PciI A'CATG_T
Cuts at: 4344 4344
Size: 5192
PfoI T'CCnGG_A
Cuts at: 251 860 1309 251
Size: 609 449 4134
Fragments arranged by size:
4134 609 449
PmeI GTTT'AAAC
Cuts at: 2191 2191
Size: 5192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 5160
PpuMI rG'GwC_Cy
Cuts at: 1566 2142 1566
Size: 576 4616
PspOMI G'GGCC_C
Cuts at: 306 539 306
Size: 233 4959
PstI C_TGCA'G
Cuts at: 758 992 2239 2924 758
Size: 234 1247 685 3026
Fragments arranged by size:
3026 1247 685 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5192
PvuII CAG'CTG
Cuts at: 777 1205 2617 2977 777
Size: 428 1412 360 2992
Fragments arranged by size:
2992 1412 428 360
RsrII CG'GwC_CG
Cuts at: 3387 3387
Size: 5192
SacI G_AGCT'C
Cuts at: 2206 2206
Size: 5192
SalI G'TCGA_C
Cuts at: 2229 2229
Size: 5192
SapI GCTCTTCn'nnn_
Cuts at: 589 3215 3425 589
Size: 2626 210 2356
Fragments arranged by size:
2626 2356 210
SbfI CC_TGCA'GG
Cuts at: 758 2239 758
Size: 1481 3711
ScaI AGT'ACT
Cuts at: 1921 4709 1921
Size: 2788 2404
SfcI C'TryA_G
Cuts at: 33 552 754 988 2235 2920 3888 4079 33
Size: 519 202 234 1247 685 968 191 1146
Fragments arranged by size:
1247 1146 968 685 519 234 202 191
SfoI GGC'GCC
Cuts at: 431 572 2624 2872 431
Size: 141 2052 248 2751
Fragments arranged by size:
2751 2052 248 141
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5192
SmaI CCC'GGG
Cuts at: 2214 2214
Size: 5192
SmlI C'TyrA_G
Cuts at: 343 826 2347 3262 3976 4238 343
Size: 483 1521 915 714 262 1297
Fragments arranged by size:
1521 1297 915 714 483 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5192
SphI G_CATG'C
Cuts at: 2245 2745 2245
Size: 500 4692
StuI AGG'CCT
Cuts at: 475 475
Size: 5192
StyI C'CwwG_G
Cuts at: 107 1016 1761 2326 2680 107
Size: 909 745 565 354 2619
Fragments arranged by size:
2619 909 745 565 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2775 2908 3367 3576 2775
Size: 133 459 209 4391
Fragments arranged by size:
4391 459 209 133
TatI w'GTAC_w
Cuts at: 510 1037 1919 4707 510
Size: 527 882 2788 995
Fragments arranged by size:
2788 995 882 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4709 4741 4709
Size: 32 5160
Tth111I GACn'n_nGTC
Cuts at: 2989 2989
Size: 5192
XbaI T'CTAG_A
Cuts at: 2223 2223
Size: 5192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 2687 447
Size: 2240 2952
XhoI C'TCGA_G
Cuts at: 343 343
Size: 5192
XmaI C'CCGG_G
Cuts at: 2212 2212
Size: 5192
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI BsaXI BsiWI BspEI BssHII BstEII BstZ17I CspCI
DraIII EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI