Restriction map for
pF1KB8692
(Circular) (Six-base) MAPSORT of: pF1KB8692.seq Check: 5194 from: 1 to: 4550
pF1KB8692 4550 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 1588 1588
Size: 4550
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 1485 4390 1485
Size: 2905 1645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 1319 2375 2807 3160 3822 1255
Size: 64 1056 432 353 662 1983
Fragments arranged by size:
1983 1056 662 432 353 64
AfeI AGC'GCT
Cuts at: 632 632
Size: 4550
AflII C'TTAA_G
Cuts at: 1090 1090
Size: 4550
AflIII A'CryG_T
Cuts at: 1902 3702 3983 1902
Size: 1800 281 2469
Fragments arranged by size:
2469 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 994 994
Size: 4550
AlwNI CAG_nnn'CTG
Cuts at: 1059 1855 3293 1059
Size: 796 1438 2316
Fragments arranged by size:
2316 1438 796
ApaLI G'TGCA_C
Cuts at: 3388 3388
Size: 4550
ApoI r'AATT_y
Cuts at: 1023 1554 3903 4159 1023
Size: 531 2349 256 1414
Fragments arranged by size:
2349 1414 531 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 275 542 1570 275
Size: 267 1028 3255
Fragments arranged by size:
3255 1028 267
AvrII C'CTAG_G
Cuts at: 1375 1375
Size: 4550
BamHI G'GATC_C
Cuts at: 477 1575 477
Size: 1098 3452
BanI G'GyrC_C
Cuts at: 617 1566 1980 2228 2263 617
Size: 949 414 248 35 2904
Fragments arranged by size:
2904 949 414 248 35
BanII G_rGCy'C
Cuts at: 448 1564 2594 448
Size: 1116 1030 2404
Fragments arranged by size:
2404 1116 1030
BbeI G_GCGC'C
Cuts at: 621 1984 2232 621
Size: 1363 248 2939
Fragments arranged by size:
2939 1363 248
BbsI GAAGACnn'nnnn_
Cuts at: 806 1262 806
Size: 456 4094
BbvCI CC'TCA_GC
Cuts at: 464 464
Size: 4550
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3504 4550 2448
Size: 1056 1046 2448
Fragments arranged by size:
2448 1056 1046
BclI T'GATC_A
Cuts at: 1028 2070 1028
Size: 1042 3508
BglII A'GATC_T
Cuts at: 1194 2065 4102 1194
Size: 871 2037 1642
Fragments arranged by size:
2037 1642 871
BlpI GC'TnA_GC
Cuts at: 1662 1662
Size: 4550
Bme1580I G_kGCm'C
Cuts at: 2175 2268 3392 2175
Size: 93 1124 3333
Fragments arranged by size:
3333 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 476 1762 1843 2178 4322 476
Size: 1286 81 335 2144 704
Fragments arranged by size:
2144 1286 704 335 81
BmtI G_CTAG'C
Cuts at: 358 358
Size: 4550
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 859
Size: 4550
Bpu10I CC'TnA_GC
Cuts at: 285 290 464 285
Size: 5 174 4371
Fragments arranged by size:
4371 174 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 1726 2605 3319 3617 921
Size: 805 879 714 298 1854
Fragments arranged by size:
1854 879 805 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4254 4254
Size: 4550
BsaAI yAC'GTr
Cuts at: 1903 1903
Size: 4550
BsaBI GATnn'nnATC
Cuts at: 6 1397 2064 4101 6
Size: 1391 667 2037 455
Fragments arranged by size:
2037 1391 667 455
BsaHI Gr'CG_yC
Cuts at: 618 1981 2229 2931 4427 618
Size: 1363 248 702 1496 741
Fragments arranged by size:
1496 1363 741 702 248
BsaWI w'CCGG_w
Cuts at: 1739 2260 2938 3349 3496 3770 1739
Size: 521 678 411 147 274 2519
Fragments arranged by size:
2519 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 832 862 832
Size: 30 4520
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 349 352 355 358 361 421 424 427
Size: 12 3 3 3 3 60 3 3
Cuts at: 427 430 433 586 589 601 604 607 610
Size: 3 3 153 3 12 3 3 3
Cuts at: 610 337
Size: 4277
Fragments arranged by size:
4277 153 60 12 12 3 3 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 978 1975 2733 3398 3806 978
Size: 997 758 665 408 1722
Fragments arranged by size:
1722 997 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1535 1535
Size: 4550
BsiEI CG_ry'CG
Cuts at: 104 2092 2138 3368 104
Size: 1988 46 1230 1286
Fragments arranged by size:
1988 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 448 1564 2342 2532 3392 448
Size: 1116 778 190 860 1606
Fragments arranged by size:
1606 1116 860 778 190
BsmI GAATG_Cn'
Cuts at: 402 429 1508 402
Size: 27 1079 3444
Fragments arranged by size:
3444 1079 27
BsmBI CGTCTCn'nnnn_
Cuts at: 311 311
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 448 1564 2175 2268 2342 2532 2594 3392 448
Size: 1116 611 93 74 190 62 798 1606
Fragments arranged by size:
1606 1116 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 261 2982 261
Size: 2721 1829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 1600 1814 2116 2497 157
Size: 1443 214 302 381 2210
Fragments arranged by size:
2210 1443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4380 4545 50
Size: 2792 54 1484 165 55
Fragments arranged by size:
2792 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2462 2462
Size: 4550
BsrFI r'CCGG_y
Cuts at: 548 2548 2729 548
Size: 2000 181 2369
Fragments arranged by size:
2369 2000 181
BssSI C'ACGA_G
Cuts at: 2821 3529 2821
Size: 708 3842
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstYI r'GATC_y
Cuts at: 1 118 477 1194 1575 2042 2065 2400 2646
Size: 117 359 717 381 467 23 335 246
Cuts at: 2646 2952 2964 3050 4102 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 717 467 449 381 359 335 306
246 117 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 689 689
Size: 4550
BtgI C'CryG_G
Cuts at: 107 107
Size: 4550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 1793 1793
Size: 4550
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 808 2256 3600 808
Size: 1448 1344 1758
Fragments arranged by size:
1758 1448 1344
EaeI y'GGCC_r
Cuts at: 2135 2309 2700 2727 4072 2135
Size: 174 391 27 1345 2613
Fragments arranged by size:
2613 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4550
EarI CTCTTCn'nnn_
Cuts at: 1425 1443 1472 2573 2783 1425
Size: 18 29 1101 210 3192
Fragments arranged by size:
3192 1101 210 29 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2758 3500 3646 4360 2758
Size: 742 146 714 2948
Fragments arranged by size:
2948 742 714 146
EcoICRI GAG'CTC
Cuts at: 446 1562 446
Size: 1116 3434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1255 1319 2375 2807 3160 3822 859
Size: 396 64 1056 432 353 662 1587
Fragments arranged by size:
1587 1056 662 432 396 353 64
EcoO109I rG'GnC_Cy
Cuts at: 169 1689 169
Size: 1520 3030
EcoRI G'AATT_C
Cuts at: 1554 1554
Size: 4550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4550
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 621 634 1984 2232 3462 4233 621
Size: 13 1350 248 1230 771 938
Fragments arranged by size:
1350 1230 938 771 248 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 980 1012 2392 2422 2424 2454 256
Size: 32 692 32 1380 30 2 30 2352
Fragments arranged by size:
2352 1380 692 32 32 30 30 2
HincII GTy'rAC
Cuts at: 559 818 1085 1589 559
Size: 259 267 504 3520
Fragments arranged by size:
3520 504 267 259
HindIII A'AGCT_T
Cuts at: 1537 1537
Size: 4550
KasI G'GCGC_C
Cuts at: 617 1980 2228 617
Size: 1363 248 2939
Fragments arranged by size:
2939 1363 248
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3309 3493 3309
Size: 184 4366
MscI TGG'CCA
Cuts at: 2311 2311
Size: 4550
MslI CAynn'nnrTG
Cuts at: 2539 2666 2539
Size: 127 4423
MspA1I CmG'CkG
Cuts at: 151 615 1661 1975 2335 3119 3364 151
Size: 464 1046 314 360 784 245 1337
Fragments arranged by size:
1337 1046 784 464 360 314 245
NaeI GCC'GGC
Cuts at: 550 2731 550
Size: 2181 2369
NarI GG'CG_CC
Cuts at: 618 1981 2229 618
Size: 1363 248 2939
Fragments arranged by size:
2939 1363 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4550
NgoMIV G'CCGG_C
Cuts at: 548 2729 548
Size: 2181 2369
NheI G'CTAG_C
Cuts at: 354 354
Size: 4550
NspI r_CATG'y
Cuts at: 1603 2103 3706 1603
Size: 500 1603 2447
Fragments arranged by size:
2447 1603 500
PciI A'CATG_T
Cuts at: 3702 3702
Size: 4550
PflMI CCAn_nnn'nTGG
Cuts at: 985 985
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1442 1474 2996 3028 1442
Size: 32 1522 32 2964
Fragments arranged by size:
2964 1522 32 32
PpuMI rG'GwC_Cy
Cuts at: 169 169
Size: 4550
PshAI GACnn'nnGTC
Cuts at: 712 712
Size: 4550
PstI C_TGCA'G
Cuts at: 1597 2282 1597
Size: 685 3865
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 151 1975 2335 151
Size: 1824 360 2366
Fragments arranged by size:
2366 1824 360
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 448 1564 448
Size: 1116 3434
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 1425 1472 2573 2783 1425
Size: 47 1101 210 3192
Fragments arranged by size:
3192 1101 210 47
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 1240 4067 1240
Size: 2827 1723
SfcI C'TryA_G
Cuts at: 33 1593 2278 3246 3437 33
Size: 1560 685 968 191 1146
Fragments arranged by size:
1560 1146 968 685 191
SfoI GGC'GCC
Cuts at: 619 1982 2230 619
Size: 1363 248 2939
Fragments arranged by size:
2939 1363 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 277 1572 277
Size: 1295 3255
SmlI C'TyrA_G
Cuts at: 900 1090 1705 2620 3334 3596 900
Size: 190 615 915 714 262 1854
Fragments arranged by size:
1854 915 714 615 262 190
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 1603 2103 1603
Size: 500 4050
StuI AGG'CCT
Cuts at: 469 670 469
Size: 201 4349
StyI C'CwwG_G
Cuts at: 107 161 1375 1684 2038 107
Size: 54 1214 309 354 2619
Fragments arranged by size:
2619 1214 354 309 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2133 2266 2725 2934 2133
Size: 133 459 209 3749
Fragments arranged by size:
3749 459 209 133
TatI w'GTAC_w
Cuts at: 306 911 1238 4065 306
Size: 605 327 2827 791
Fragments arranged by size:
2827 791 605 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4067 4099 4067
Size: 32 4518
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4550
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2045 2045
Size: 4550
XmaI C'CCGG_G
Cuts at: 275 1570 275
Size: 1295 3255
XmnI GAAnn'nnTTC
Cuts at: 1118 1118
Size: 4550
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AleI AlfI AloI ApaI AscI
BaeI BcgI BfrBI BglI BmgBI BsiWI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PfoI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI