Restriction map for
pF1KB8693
(Circular) (Six-base) MAPSORT of: pF1KB8693.seq Check: 534 from: 1 to: 4727
pF1KB8693 4727 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AatII G_ACGT'C
Cuts at: 638 638
Size: 4727
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 1060 1201 1351 2552 2984 3337 3999 146
Size: 914 141 150 1201 432 353 662 874
Fragments arranged by size:
1201 914 874 662 432 353 150 141
AfeI AGC'GCT
Cuts at: 820 820
Size: 4727
AflIII A'CryG_T
Cuts at: 413 1172 1238 2079 3879 4160 413
Size: 759 66 841 1800 281 980
Fragments arranged by size:
1800 980 841 759 281 66
AhdI GACnn_n'nnGTC
Cuts at: 493 493
Size: 4727
AlwNI CAG_nnn'CTG
Cuts at: 186 2032 3470 186
Size: 1846 1438 1443
Fragments arranged by size:
1846 1443 1438
ApaLI G'TGCA_C
Cuts at: 3565 3565
Size: 4727
ApoI r'AATT_y
Cuts at: 865 1731 4080 4336 865
Size: 866 2349 256 1256
Fragments arranged by size:
2349 1256 866 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 119 1361 1747 119
Size: 1242 386 3099
Fragments arranged by size:
3099 1242 386
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 1651 1743 2157 2405 2440 1651
Size: 92 414 248 35 3938
Fragments arranged by size:
3938 414 248 92 35
BanII G_rGCy'C
Cuts at: 911 1166 1741 2771 911
Size: 255 575 1030 2867
Fragments arranged by size:
2867 1030 575 255
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbsI GAAGACnn'nnnn_
Cuts at: 1339 1391 1339
Size: 52 4675
BciVI GTATCCnnnnn_n'
Cuts at: 863 2625 3681 4727 863
Size: 1762 1056 1046 863
Fragments arranged by size:
1762 1056 1046 863
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglI GCCn_nnn'nGGC
Cuts at: 547 1597 547
Size: 1050 3677
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1255 1587 1839 1255
Size: 332 252 4143
Fragments arranged by size:
4143 332 252
Bme1580I G_kGCm'C
Cuts at: 1238 2352 2445 3569 1238
Size: 1114 93 1124 2396
Fragments arranged by size:
2396 1124 1114 93
BmgBI CAC'GTC
Cuts at: 1175 1175
Size: 4727
BmrI ACTGGGnnnn_n'
Cuts at: 496 1487 1634 1939 2020 2355 4499 496
Size: 991 147 305 81 335 2144 724
Fragments arranged by size:
2144 991 724 335 305 147 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 231 1338 1602 3094 231
Size: 1107 264 1492 1864
Fragments arranged by size:
1864 1492 1107 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 313 403 1011 1690 226
Size: 87 90 608 679 3263
Fragments arranged by size:
3263 679 608 90 87
Bpu10I CC'TnA_GC
Cuts at: 576 576
Size: 4727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 2782 3496 3794 1903
Size: 879 714 298 2836
Fragments arranged by size:
2836 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4431 4431
Size: 4727
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 635 2158 2406 3108 4604 635
Size: 1523 248 702 1496 758
Fragments arranged by size:
1523 1496 758 702 248
BsaWI w'CCGG_w
Cuts at: 1648 1697 1916 2437 3115 3526 3673 3947 1648
Size: 49 219 521 678 411 147 274 2428
Fragments arranged by size:
2428 678 521 411 274 219 147 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1335 1365 1335
Size: 30 4697
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 658 783 829 1282 1330 1356 223
Size: 435 125 46 453 48 26 3594
Fragments arranged by size:
3594 453 435 125 48 46 26
BseYI C'CCAG_C
Cuts at: 186 815 1106 1512 2152 2910 3575 3983 186
Size: 629 291 406 640 758 665 408 930
Fragments arranged by size:
930 758 665 640 629 408 406 291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 626 501
Size: 125 4602
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3545 104
Size: 2165 46 1230 1286
Fragments arranged by size:
2165 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 657 1741 2519 2709 3569 657
Size: 1084 778 190 860 1815
Fragments arranged by size:
1815 1084 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1395 1395
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 657 911 1166 1238 1741 2352 2445 2519 2709
Size: 254 255 72 503 611 93 74 190
Cuts at: 2709 2771 3569 657
Size: 62 798 1815
Fragments arranged by size:
1815 798 611 503 255 254 190 93
74 72 62
BspEI T'CCGG_A
Cuts at: 1697 1697
Size: 4727
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 524 1777 1991 2293 2674 524
Size: 1253 214 302 381 2577
Fragments arranged by size:
2577 1253 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3019 3073 4557 4722 50
Size: 2969 54 1484 165 55
Fragments arranged by size:
2969 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1719 2639 1719
Size: 920 3807
BsrFI r'CCGG_y
Cuts at: 112 253 2725 2906 112
Size: 141 2472 181 1933
Fragments arranged by size:
2472 1933 181 141
BssSI C'ACGA_G
Cuts at: 2998 3706 2998
Size: 708 4019
BstAPI GCAn_nnn'nTGC
Cuts at: 613 1581 2104 613
Size: 968 523 3236
Fragments arranged by size:
3236 968 523
BstBI TT'CG_AA
Cuts at: 660 3088 660
Size: 2428 2299
BstYI r'GATC_y
Cuts at: 1 1011 1193 1752 2219 2242 2577 2823 3129
Size: 1010 182 559 467 23 335 246 306
Cuts at: 3129 3141 3227 4279 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1010 559 467 449 335 306 246
182 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 179 179
Size: 4727
BtgI C'CryG_G
Cuts at: 107 1413 1499 1683 107
Size: 1306 86 184 3151
Fragments arranged by size:
3151 1306 184 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 1970 1970
Size: 4727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1413 1448 1413
Size: 35 4692
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DraIII CAC_nnn'GTG
Cuts at: 375 375
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3777 2433
Size: 1344 3383
EaeI y'GGCC_r
Cuts at: 110 303 354 539 2312 2486 2877 2904 4249
Size: 193 51 185 1773 174 391 27 1345
Cuts at: 4249 110
Size: 588
Fragments arranged by size:
1773 1345 588 391 193 185 174 51
27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 165 277 662 1129 1234 1342 2750 2960 165
Size: 112 385 467 105 108 1408 210 1932
Fragments arranged by size:
1932 1408 467 385 210 112 108 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3677 3823 4537 2935
Size: 742 146 714 3125
Fragments arranged by size:
3125 742 714 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 226 313 403 1011 1060 1201 1351 1690
Size: 80 87 90 608 49 141 150 339
Cuts at: 1690 2552 2984 3337 3999 146
Size: 862 432 353 662 874
Fragments arranged by size:
874 862 662 608 432 353 339 150
141 90 87 80 49
EcoNI CCTnn'n_nnAGG
Cuts at: 514 514
Size: 4727
EcoO109I rG'GnC_Cy
Cuts at: 149 1662 1866 149
Size: 1513 204 3010
Fragments arranged by size:
3010 1513 204
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
EcoRV GAT'ATC
Cuts at: 348 348
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 119 822 1525 2161 2409 3639 4410 119
Size: 703 703 636 248 1230 771 436
Fragments arranged by size:
1230 771 703 703 636 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 479 511 960 984 992 1016 2569 2599 2601
Size: 32 449 24 8 24 1553 30 2
Cuts at: 2601 2631 479
Size: 30 2575
Fragments arranged by size:
2575 1553 449 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 1062 1766 1062
Size: 704 4023
HpaI GTT'AAC
Cuts at: 1062 1062
Size: 4727
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1671 3486 3670 1671
Size: 1815 184 2728
Fragments arranged by size:
2728 1815 184
MscI TGG'CCA
Cuts at: 356 2488 356
Size: 2132 2595
MslI CAynn'nnrTG
Cuts at: 1393 2716 2843 1393
Size: 1323 127 3277
Fragments arranged by size:
3277 1323 127
MspA1I CmG'CkG
Cuts at: 136 248 1079 1838 2152 2512 3296 3541 136
Size: 112 831 759 314 360 784 245 1322
Fragments arranged by size:
1322 831 784 759 360 314 245 112
NaeI GCC'GGC
Cuts at: 114 2908 114
Size: 2794 1933
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NcoI C'CATG_G
Cuts at: 107 107
Size: 4727
NgoMIV G'CCGG_C
Cuts at: 112 2906 112
Size: 2794 1933
NspI r_CATG'y
Cuts at: 1242 1398 1780 2280 3883 1242
Size: 156 382 500 1603 2086
Fragments arranged by size:
2086 1603 500 382 156
PciI A'CATG_T
Cuts at: 1238 3879 1238
Size: 2641 2086
PfoI T'CCnGG_A
Cuts at: 217 380 217
Size: 163 4564
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 149 149
Size: 4727
PstI C_TGCA'G
Cuts at: 1577 1774 2459 1577
Size: 197 685 3845
Fragments arranged by size:
3845 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 662 2750 2960 662
Size: 2088 210 2429
Fragments arranged by size:
2429 2088 210
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SfcI C'TryA_G
Cuts at: 33 1573 1770 2455 3423 3614 33
Size: 1540 197 685 968 191 1146
Fragments arranged by size:
1540 1146 968 685 197 191
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1363 1749 1363
Size: 386 4341
SmlI C'TyrA_G
Cuts at: 1882 2797 3511 3773 1882
Size: 915 714 262 2836
Fragments arranged by size:
2836 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1398 1780 2280 1398
Size: 382 500 3845
Fragments arranged by size:
3845 500 382
StyI C'CwwG_G
Cuts at: 107 236 1431 1718 1861 2215 107
Size: 129 1195 287 143 354 2619
Fragments arranged by size:
2619 1195 354 287 143 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2310 2443 2902 3111 2310
Size: 133 459 209 3926
Fragments arranged by size:
3926 459 209 133
TatI w'GTAC_w
Cuts at: 4242 4242
Size: 4727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 4695
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 4727
XmaI C'CCGG_G
Cuts at: 1361 1747 1361
Size: 386 4341
ZraI GAC'GTC
Cuts at: 636 636
Size: 4727
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I ClaI FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI