Restriction map for
pF1KB8694
(Circular) (Six-base) MAPSORT of: pF1KB8694.seq Check: 9364 from: 1 to: 4478
pF1KB8694 4478 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 561 1516 561
Size: 955 3523
Acc65I G'GTAC_C
Cuts at: 1494 1494
Size: 4478
AclI AA'CG_TT
Cuts at: 4318 4318
Size: 4478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 787 1340 2303 2735 3088 3750 736
Size: 51 553 963 432 353 662 1464
Fragments arranged by size:
1464 963 662 553 432 353 51
AflIII A'CryG_T
Cuts at: 1311 1830 3630 3911 1311
Size: 519 1800 281 1878
Fragments arranged by size:
1878 1800 519 281
AlwNI CAG_nnn'CTG
Cuts at: 316 923 1783 3221 316
Size: 607 860 1438 1573
Fragments arranged by size:
1573 1438 860 607
ApaLI G'TGCA_C
Cuts at: 3316 3316
Size: 4478
ApoI r'AATT_y
Cuts at: 850 1482 3831 4087 850
Size: 632 2349 256 1241
Fragments arranged by size:
2349 1241 632 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4478
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
AvaI C'yCGr_G
Cuts at: 118 410 695 1498 118
Size: 292 285 803 3098
Fragments arranged by size:
3098 803 292 285
AvrII C'CTAG_G
Cuts at: 1014 1014
Size: 4478
BamHI G'GATC_C
Cuts at: 1018 1503 1018
Size: 485 3993
BanI G'GyrC_C
Cuts at: 114 334 1494 1908 2156 2191 114
Size: 220 1160 414 248 35 2401
Fragments arranged by size:
2401 1160 414 248 220 35
BanII G_rGCy'C
Cuts at: 609 1492 2522 609
Size: 883 1030 2565
Fragments arranged by size:
2565 1030 883
BbeI G_GCGC'C
Cuts at: 338 1912 2160 338
Size: 1574 248 2656
Fragments arranged by size:
2656 1574 248
BciVI GTATCCnnnnn_n'
Cuts at: 852 2376 3432 4478 852
Size: 1524 1056 1046 852
Fragments arranged by size:
1524 1056 1046 852
BclI T'GATC_A
Cuts at: 1998 1998
Size: 4478
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4478
BglII A'GATC_T
Cuts at: 428 1993 4030 428
Size: 1565 2037 876
Fragments arranged by size:
2037 1565 876
BlpI GC'TnA_GC
Cuts at: 1590 1590
Size: 4478
Bme1580I G_kGCm'C
Cuts at: 2103 2196 3320 2103
Size: 93 1124 3261
Fragments arranged by size:
3261 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1690 1771 2106 4250 1690
Size: 81 335 2144 1918
Fragments arranged by size:
2144 1918 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2845 2845
Size: 4478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1412 1463 388
Size: 1024 51 3403
Fragments arranged by size:
3403 1024 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 1654 2533 3247 3545 621
Size: 1033 879 714 298 1554
Fragments arranged by size:
1554 1033 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 364 4182 364
Size: 3818 660
BsaAI yAC'GTr
Cuts at: 1831 1831
Size: 4478
BsaBI GATnn'nnATC
Cuts at: 6 1992 4029 6
Size: 1986 2037 455
Fragments arranged by size:
2037 1986 455
BsaHI Gr'CG_yC
Cuts at: 335 1909 2157 2859 4355 335
Size: 1574 248 702 1496 458
Fragments arranged by size:
1574 1496 702 458 248
BsaWI w'CCGG_w
Cuts at: 1186 1667 2188 2866 3277 3424 3698 1186
Size: 481 521 678 411 147 274 1966
Fragments arranged by size:
1966 678 521 481 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 4478
BseYI C'CCAG_C
Cuts at: 149 1177 1903 2661 3326 3734 149
Size: 1028 726 758 665 408 893
Fragments arranged by size:
1028 893 758 726 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 4478
BsiEI CG_ry'CG
Cuts at: 104 140 639 2020 2066 3296 104
Size: 36 499 1381 46 1230 1286
Fragments arranged by size:
1381 1286 1230 499 46 36
BsiHKAI G_wGCw'C
Cuts at: 609 1492 2270 2460 3320 609
Size: 883 778 190 860 1767
Fragments arranged by size:
1767 883 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 609 1492 2103 2196 2270 2460 2522 3320 609
Size: 883 611 93 74 190 62 798 1767
Fragments arranged by size:
1767 883 798 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1186 1186
Size: 4478
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1528 1742 2044 2425 1528
Size: 214 302 381 3581
Fragments arranged by size:
3581 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2770 2824 4308 4473 50
Size: 2720 54 1484 165 55
Fragments arranged by size:
2720 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2390 2390
Size: 4478
BsrFI r'CCGG_y
Cuts at: 2476 2657 2476
Size: 181 4297
BssSI C'ACGA_G
Cuts at: 2749 3457 2749
Size: 708 3770
BstAPI GCAn_nnn'nTGC
Cuts at: 1855 1855
Size: 4478
BstBI TT'CG_AA
Cuts at: 2839 2839
Size: 4478
BstYI r'GATC_y
Cuts at: 1 428 677 1018 1503 1970 1993 2328 2574
Size: 427 249 341 485 467 23 335 246
Cuts at: 2574 2880 2892 2978 4030 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 485 467 449 427 341 335 306
249 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 987 107
Size: 880 3598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2428 2609 2772 2428
Size: 181 163 4134
Fragments arranged by size:
4134 181 163
BtsI GCAGTG_nn'
Cuts at: 361 971 1721 361
Size: 610 750 3118
Fragments arranged by size:
3118 750 610
DraI TTT'AAA
Cuts at: 186 1477 186
Size: 1291 3187
DrdI GACnn_nn'nnGTC
Cuts at: 144 998 2184 3528 144
Size: 854 1186 1344 1094
Fragments arranged by size:
1344 1186 1094 854
EaeI y'GGCC_r
Cuts at: 2063 2237 2628 2655 4000 2063
Size: 174 391 27 1345 2541
Fragments arranged by size:
2541 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2063 2063
Size: 4478
EarI CTCTTCn'nnn_
Cuts at: 158 721 1234 2501 2711 158
Size: 563 513 1267 210 1925
Fragments arranged by size:
1925 1267 563 513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2686 3428 3574 4288 2686
Size: 742 146 714 2876
Fragments arranged by size:
2876 742 714 146
EcoICRI GAG'CTC
Cuts at: 607 1490 607
Size: 883 3595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 736 787 1340 1412 1463 2303 2735 3088
Size: 348 51 553 72 51 840 432 353
Cuts at: 3088 3750 388
Size: 662 1116
Fragments arranged by size:
1116 840 662 553 432 353 348 72
51 51
EcoO109I rG'GnC_Cy
Cuts at: 455 1617 455
Size: 1162 3316
EcoRI G'AATT_C
Cuts at: 1482 1482
Size: 4478
EcoRV GAT'ATC
Cuts at: 297 297
Size: 4478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4478
FspI TGC'GCA
Cuts at: 2259 2259
Size: 4478
HaeII r_GCGC'y
Cuts at: 338 1014 1185 1912 2160 3390 4161 338
Size: 676 171 727 248 1230 771 655
Fragments arranged by size:
1230 771 727 676 655 248 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 490 522 2320 2350 2352 2382 490
Size: 32 1798 30 2 30 2586
Fragments arranged by size:
2586 1798 32 30 30 2
HincII GTy'rAC
Cuts at: 562 672 1116 1517 562
Size: 110 444 401 3523
Fragments arranged by size:
3523 444 401 110
HindIII A'AGCT_T
Cuts at: 218 218
Size: 4478
KasI G'GCGC_C
Cuts at: 334 1908 2156 334
Size: 1574 248 2656
Fragments arranged by size:
2656 1574 248
KpnI G_GTAC'C
Cuts at: 1498 1498
Size: 4478
MluI A'CGCG_T
Cuts at: 1311 3911 1311
Size: 2600 1878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 160 1300 3237 3421 160
Size: 1140 1937 184 1217
Fragments arranged by size:
1937 1217 1140 184
MscI TGG'CCA
Cuts at: 2239 2239
Size: 4478
MslI CAynn'nnrTG
Cuts at: 1103 2467 2594 1103
Size: 1364 127 2987
Fragments arranged by size:
2987 1364 127
MspA1I CmG'CkG
Cuts at: 1589 1903 2263 3047 3292 1589
Size: 314 360 784 245 2775
Fragments arranged by size:
2775 784 360 314 245
NaeI GCC'GGC
Cuts at: 2659 2659
Size: 4478
NarI GG'CG_CC
Cuts at: 335 1909 2157 335
Size: 1574 248 2656
Fragments arranged by size:
2656 1574 248
NcoI C'CATG_G
Cuts at: 107 987 107
Size: 880 3598
NgoMIV G'CCGG_C
Cuts at: 2657 2657
Size: 4478
NruI TCG'CGA
Cuts at: 1380 1380
Size: 4478
NspI r_CATG'y
Cuts at: 1531 2031 3634 1531
Size: 500 1603 2375
Fragments arranged by size:
2375 1603 500
PasI CC'CwG_GG
Cuts at: 450 450
Size: 4478
PciI A'CATG_T
Cuts at: 3630 3630
Size: 4478
PmeI GTTT'AAAC
Cuts at: 1477 1477
Size: 4478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 4446
PpuMI rG'GwC_Cy
Cuts at: 455 455
Size: 4478
PstI C_TGCA'G
Cuts at: 919 1525 2210 919
Size: 606 685 3187
Fragments arranged by size:
3187 685 606
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4478
PvuII CAG'CTG
Cuts at: 1903 2263 1903
Size: 360 4118
RsrII CG'GwC_CG
Cuts at: 2673 2673
Size: 4478
SacI G_AGCT'C
Cuts at: 609 1492 609
Size: 883 3595
SalI G'TCGA_C
Cuts at: 560 1515 560
Size: 955 3523
SapI GCTCTTCn'nnn_
Cuts at: 2501 2711 2501
Size: 210 4268
SbfI CC_TGCA'GG
Cuts at: 1525 1525
Size: 4478
ScaI AGT'ACT
Cuts at: 3995 3995
Size: 4478
SexAI A'CCwGG_T
Cuts at: 866 1266 866
Size: 400 4078
SfcI C'TryA_G
Cuts at: 33 915 1521 2206 3174 3365 33
Size: 882 606 685 968 191 1146
Fragments arranged by size:
1146 968 882 685 606 191
SfoI GGC'GCC
Cuts at: 336 1910 2158 336
Size: 1574 248 2656
Fragments arranged by size:
2656 1574 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
SmaI CCC'GGG
Cuts at: 1500 1500
Size: 4478
SmlI C'TyrA_G
Cuts at: 600 695 1633 2548 3262 3524 600
Size: 95 938 915 714 262 1554
Fragments arranged by size:
1554 938 915 714 262 95
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4478
SphI G_CATG'C
Cuts at: 1531 2031 1531
Size: 500 3978
SspI AAT'ATT
Cuts at: 829 829
Size: 4478
StyI C'CwwG_G
Cuts at: 107 987 1014 1040 1139 1612 1966 107
Size: 880 27 26 99 473 354 2619
Fragments arranged by size:
2619 880 473 354 99 27 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 184 653 2061 2194 2653 2862 154
Size: 30 469 1408 133 459 209 1770
Fragments arranged by size:
1770 1408 469 459 209 133 30
TatI w'GTAC_w
Cuts at: 3993 3993
Size: 4478
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1455 1487 3995 4027 1455
Size: 32 2508 32 1906
Fragments arranged by size:
2508 1906 32 32
Tth111I GACn'n_nGTC
Cuts at: 2275 2275
Size: 4478
XbaI T'CTAG_A
Cuts at: 1509 1509
Size: 4478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1083 1973 1083
Size: 890 3588
XhoI C'TCGA_G
Cuts at: 695 695
Size: 4478
XmaI C'CCGG_G
Cuts at: 1498 1498
Size: 4478
XmnI GAAnn'nnTTC
Cuts at: 269 498 592 709 269
Size: 229 94 117 4038
Fragments arranged by size:
4038 229 117 94
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI