Restriction map for
pF1KB8697
(Circular) (Six-base) MAPSORT of: pF1KB8697.seq Check: 6249 from: 1 to: 4751
pF1KB8697 4751 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 794 794
Size: 4751
AccI GT'mk_AC
Cuts at: 1789 1789
Size: 4751
Acc65I G'GTAC_C
Cuts at: 1767 1767
Size: 4751
AclI AA'CG_TT
Cuts at: 4591 4591
Size: 4751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2576 3008 3361 4023 1276
Size: 1300 432 353 662 2004
Fragments arranged by size:
2004 1300 662 432 353
AflIII A'CryG_T
Cuts at: 2103 3903 4184 2103
Size: 1800 281 2670
Fragments arranged by size:
2670 1800 281
AgeI A'CCGG_T
Cuts at: 1403 1403
Size: 4751
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1283 1283
Size: 4751
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1294 1326 1294
Size: 32 4719
AlwNI CAG_nnn'CTG
Cuts at: 1113 1569 2056 3494 1113
Size: 456 487 1438 2370
Fragments arranged by size:
2370 1438 487 456
ApaI G_GGCC'C
Cuts at: 309 391 897 309
Size: 82 506 4163
Fragments arranged by size:
4163 506 82
ApaLI G'TGCA_C
Cuts at: 3589 3589
Size: 4751
ApoI r'AATT_y
Cuts at: 720 1755 4104 4360 720
Size: 1035 2349 256 1111
Fragments arranged by size:
2349 1111 1035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4751
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4751
AvaI C'yCGr_G
Cuts at: 187 896 1771 187
Size: 709 875 3167
Fragments arranged by size:
3167 875 709
BamHI G'GATC_C
Cuts at: 522 1776 522
Size: 1254 3497
BanI G'GyrC_C
Cuts at: 1169 1767 2181 2429 2464 1169
Size: 598 414 248 35 3456
Fragments arranged by size:
3456 598 414 248 35
BanII G_rGCy'C
Cuts at: 198 282 309 391 897 1591 1638 1730 1765
Size: 84 27 82 506 694 47 92 35
Cuts at: 1765 2795 198
Size: 1030 2154
Fragments arranged by size:
2154 1030 694 506 92 84 82 47
35 27
BbeI G_GCGC'C
Cuts at: 2185 2433 2185
Size: 248 4503
BbsI GAAGACnn'nnnn_
Cuts at: 353 1077 353
Size: 724 4027
BbvCI CC'TCA_GC
Cuts at: 1448 1448
Size: 4751
BciVI GTATCCnnnnn_n'
Cuts at: 2649 3705 4751 2649
Size: 1056 1046 2649
Fragments arranged by size:
2649 1056 1046
BclI T'GATC_A
Cuts at: 2271 2271
Size: 4751
BglI GCCn_nnn'nGGC
Cuts at: 292 814 1626 292
Size: 522 812 3417
Fragments arranged by size:
3417 812 522
BglII A'GATC_T
Cuts at: 2266 4303 2266
Size: 2037 2714
BlpI GC'TnA_GC
Cuts at: 331 1863 331
Size: 1532 3219
Bme1580I G_kGCm'C
Cuts at: 309 391 408 897 1527 2376 2469 3593 309
Size: 82 17 489 630 849 93 1124 1467
Fragments arranged by size:
1467 1124 849 630 489 93 82 17
BmrI ACTGGGnnnn_n'
Cuts at: 1724 1963 2044 2379 4523 1724
Size: 239 81 335 2144 1952
Fragments arranged by size:
2144 1952 335 239 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 945 1008 1749 3118 945
Size: 63 741 1369 2578
Fragments arranged by size:
2578 1369 741 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 727 1114 1402 1498 1528 470
Size: 257 387 288 96 30 3693
Fragments arranged by size:
3693 387 288 257 96 30
Bpu10I CC'TnA_GC
Cuts at: 1448 1448
Size: 4751
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 850 856 1018 1459 1622 1706 1927 2806
Size: 584 6 162 441 163 84 221 879
Cuts at: 2806 3520 3818 266
Size: 714 298 1199
Fragments arranged by size:
1199 879 714 584 441 298 221 163
162 84 6
BsaI GGTCTCn'nnnn_
Cuts at: 557 563 1240 4455 557
Size: 6 677 3215 853
Fragments arranged by size:
3215 853 677 6
BsaAI yAC'GTr
Cuts at: 2104 2104
Size: 4751
BsaBI GATnn'nnATC
Cuts at: 6 2265 4302 6
Size: 2259 2037 455
Fragments arranged by size:
2259 2037 455
BsaHI Gr'CG_yC
Cuts at: 2182 2430 3132 4628 2182
Size: 248 702 1496 2305
Fragments arranged by size:
2305 1496 702 248
BsaWI w'CCGG_w
Cuts at: 174 716 1403 1940 2461 3139 3550 3697 3971
Size: 542 687 537 521 678 411 147 274
Cuts at: 3971 174
Size: 954
Fragments arranged by size:
954 687 678 542 537 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 850 880 1501 1531 610
Size: 30 210 30 621 30 3830
Fragments arranged by size:
3830 621 210 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 251 340 679 724 916 919 922 1081 1306
Size: 89 339 45 192 3 3 159 225
Cuts at: 1306 1465 1629 251
Size: 159 164 3373
Fragments arranged by size:
3373 339 225 192 164 159 159 89
45 3 3
BseYI C'CCAG_C
Cuts at: 208 1645 2176 2934 3599 4007 208
Size: 1437 531 758 665 408 952
Fragments arranged by size:
1437 952 758 665 531 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4751
BsiEI CG_ry'CG
Cuts at: 104 2293 2339 3569 104
Size: 2189 46 1230 1286
Fragments arranged by size:
2189 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 361 716 800 1689 1696 1765 2543 2733 3593
Size: 355 84 889 7 69 778 190 860
Cuts at: 3593 361
Size: 1519
Fragments arranged by size:
1519 889 860 778 355 190 84 69
7
Bsp1286I G_dGCh'C
Cuts at: 198 282 309 361 391 408 716 800 897
Size: 84 27 52 30 17 308 84 97
Cuts at: 897 1527 1591 1638 1689 1696 1730 1765 2376
Size: 630 64 47 51 7 34 35 611
Cuts at: 2376 2469 2543 2733 2795 3593 198
Size: 93 74 190 62 798 1356
Fragments arranged by size:
1356 798 630 611 308 190 97 93
84 84 74 64 62 52 51 47
35 34 30 27 17 7
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 794 1479 1801 2015 2317 2698 794
Size: 685 322 214 302 381 2847
Fragments arranged by size:
2847 685 381 322 302 214
BsrBI CCG'CTC
Cuts at: 50 204 866 1167 3043 3097 4581 4746 50
Size: 154 662 301 1876 54 1484 165 55
Fragments arranged by size:
1876 1484 662 301 165 154 55 54
BsrDI GCAATG_nn'
Cuts at: 1548 2663 1548
Size: 1115 3636
BsrFI r'CCGG_y
Cuts at: 417 1403 2749 2930 417
Size: 986 1346 181 2238
Fragments arranged by size:
2238 1346 986 181
BssSI C'ACGA_G
Cuts at: 3022 3730 3022
Size: 708 4043
BstAPI GCAn_nnn'nTGC
Cuts at: 2128 2128
Size: 4751
BstBI TT'CG_AA
Cuts at: 3112 3112
Size: 4751
BstEII G'GTnAC_C
Cuts at: 924 924
Size: 4751
BstXI CCAn_nnnn'nTGG
Cuts at: 609 609
Size: 4751
BstYI r'GATC_y
Cuts at: 1 522 539 1776 2243 2266 2601 2847 3153
Size: 521 17 1237 467 23 335 246 306
Cuts at: 3153 3165 3251 4303 1
Size: 12 86 1052 449
Fragments arranged by size:
1237 1052 521 467 449 335 306 246
86 23 17 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2701 2882 3045 2701
Size: 181 163 4407
Fragments arranged by size:
4407 181 163
BtsI GCAGTG_nn'
Cuts at: 1525 1994 1525
Size: 469 4282
DraI TTT'AAA
Cuts at: 1750 1750
Size: 4751
DraIII CAC_nnn'GTG
Cuts at: 412 412
Size: 4751
DrdI GACnn_nn'nnGTC
Cuts at: 2457 3801 2457
Size: 1344 3407
EaeI y'GGCC_r
Cuts at: 1581 2336 2510 2901 2928 4273 1581
Size: 755 174 391 27 1345 2059
Fragments arranged by size:
2059 1345 755 391 174 27
EagI C'GGCC_G
Cuts at: 2336 2336
Size: 4751
EarI CTCTTCn'nnn_
Cuts at: 530 1157 2774 2984 530
Size: 627 1617 210 2297
Fragments arranged by size:
2297 1617 627 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1725 2959 3701 3847 4561 1725
Size: 1234 742 146 714 1915
Fragments arranged by size:
1915 1234 742 714 146
EcoICRI GAG'CTC
Cuts at: 1763 1763
Size: 4751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 727 1114 1276 1402 1498 1528 2576 3008
Size: 257 387 162 126 96 30 1048 432
Cuts at: 3008 3361 4023 470
Size: 353 662 1198
Fragments arranged by size:
1198 1048 662 432 387 353 257 162
126 96 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1198 1737 1198
Size: 539 4212
EcoO109I rG'GnC_Cy
Cuts at: 184 241 305 306 387 388 1098 1431 1482
Size: 57 64 1 81 1 710 333 51
Cuts at: 1482 1566 1627 1890 184
Size: 84 61 263 3045
Fragments arranged by size:
3045 710 333 263 84 81 64 61
57 51 1 1
EcoRI G'AATT_C
Cuts at: 720 1755 720
Size: 1035 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4751
FspI TGC'GCA
Cuts at: 2532 2532
Size: 4751
HaeII r_GCGC'y
Cuts at: 1672 2185 2433 3663 4434 1672
Size: 513 248 1230 771 1989
Fragments arranged by size:
1989 1230 771 513 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1704 1736 2593 2623 2625 2655 1704
Size: 32 857 30 2 30 3800
Fragments arranged by size:
3800 857 32 30 30 2
HincII GTy'rAC
Cuts at: 1790 1790
Size: 4751
HindIII A'AGCT_T
Cuts at: 826 826
Size: 4751
KasI G'GCGC_C
Cuts at: 2181 2429 2181
Size: 248 4503
KpnI G_GTAC'C
Cuts at: 1771 1771
Size: 4751
MluI A'CGCG_T
Cuts at: 4184 4184
Size: 4751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 540 1524 3510 3694 540
Size: 984 1986 184 1597
Fragments arranged by size:
1986 1597 984 184
MscI TGG'CCA
Cuts at: 1583 2512 1583
Size: 929 3822
MslI CAynn'nnrTG
Cuts at: 2740 2867 2740
Size: 127 4624
MspA1I CmG'CkG
Cuts at: 765 855 891 963 1113 1176 1862 2176 2536
Size: 90 36 72 150 63 686 314 360
Cuts at: 2536 3320 3565 765
Size: 784 245 1951
Fragments arranged by size:
1951 784 686 360 314 245 150 90
72 63 36
NaeI GCC'GGC
Cuts at: 419 2932 419
Size: 2513 2238
NarI GG'CG_CC
Cuts at: 2182 2430 2182
Size: 248 4503
NgoMIV G'CCGG_C
Cuts at: 417 2930 417
Size: 2513 2238
NspI r_CATG'y
Cuts at: 1804 2304 3907 1804
Size: 500 1603 2648
Fragments arranged by size:
2648 1603 500
PasI CC'CwG_GG
Cuts at: 1655 1655
Size: 4751
PciI A'CATG_T
Cuts at: 3903 3903
Size: 4751
PflMI CCAn_nnn'nTGG
Cuts at: 1506 1506
Size: 4751
PfoI T'CCnGG_A
Cuts at: 445 1463 445
Size: 1018 3733
PmeI GTTT'AAAC
Cuts at: 1750 1750
Size: 4751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4719
PpuMI rG'GwC_Cy
Cuts at: 184 241 1098 1566 184
Size: 57 857 468 3369
Fragments arranged by size:
3369 857 468 57
PspOMI G'GGCC_C
Cuts at: 305 387 893 305
Size: 82 506 4163
Fragments arranged by size:
4163 506 82
PstI C_TGCA'G
Cuts at: 228 854 968 1490 1532 1798 2483 228
Size: 626 114 522 42 266 685 2496
Fragments arranged by size:
2496 685 626 522 266 114 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4751
PvuII CAG'CTG
Cuts at: 765 963 1113 2176 2536 765
Size: 198 150 1063 360 2980
Fragments arranged by size:
2980 1063 360 198 150
RsrII CG'GwC_CG
Cuts at: 2946 2946
Size: 4751
SacI G_AGCT'C
Cuts at: 1765 1765
Size: 4751
SalI G'TCGA_C
Cuts at: 1788 1788
Size: 4751
SapI GCTCTTCn'nnn_
Cuts at: 1157 2774 2984 1157
Size: 1617 210 2924
Fragments arranged by size:
2924 1617 210
SbfI CC_TGCA'GG
Cuts at: 1490 1798 1490
Size: 308 4443
ScaI AGT'ACT
Cuts at: 4268 4268
Size: 4751
SfcI C'TryA_G
Cuts at: 33 224 745 850 964 1486 1528 1794 2479
Size: 191 521 105 114 522 42 266 685
Cuts at: 2479 3447 3638 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 522 521 266 191 191
114 105 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 814 1626 814
Size: 812 3939
SfoI GGC'GCC
Cuts at: 2183 2431 2183
Size: 248 4503
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4751
SmaI CCC'GGG
Cuts at: 898 1773 898
Size: 875 3876
SmlI C'TyrA_G
Cuts at: 281 829 871 1033 1438 1637 1721 1906 2821
Size: 548 42 162 405 199 84 185 915
Cuts at: 2821 3535 3797 281
Size: 714 262 1235
Fragments arranged by size:
1235 915 714 548 405 262 199 185
162 84 42
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4751
SphI G_CATG'C
Cuts at: 1804 2304 1804
Size: 500 4251
StyI C'CwwG_G
Cuts at: 515 602 1885 2239 515
Size: 87 1283 354 3027
Fragments arranged by size:
3027 1283 354 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 581 1659 2334 2467 2926 3135 581
Size: 1078 675 133 459 209 2197
Fragments arranged by size:
2197 1078 675 459 209 133
TatI w'GTAC_w
Cuts at: 4266 4266
Size: 4751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 850 882 1501 1533 4268 4300 850
Size: 32 619 32 2735 32 1301
Fragments arranged by size:
2735 1301 619 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 556 1081 2548 556
Size: 525 1467 2759
Fragments arranged by size:
2759 1467 525
XbaI T'CTAG_A
Cuts at: 1782 1782
Size: 4751
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2246 2246
Size: 4751
XmaI C'CCGG_G
Cuts at: 896 1771 896
Size: 875 3876
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstZ17I Bsu36I BtgI ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI