Restriction map for
pF1KB8698
(Circular) (Six-base) MAPSORT of: pF1KB8698.seq Check: 8366 from: 1 to: 4868
pF1KB8698 4868 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1906 1906
Size: 4868
Acc65I G'GTAC_C
Cuts at: 1719 1884 1719
Size: 165 4703
AclI AA'CG_TT
Cuts at: 4708 4708
Size: 4868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 1485 2693 3125 3478 4140 1366
Size: 119 1208 432 353 662 2094
Fragments arranged by size:
2094 1208 662 432 353 119
AflII C'TTAA_G
Cuts at: 222 222
Size: 4868
AflIII A'CryG_T
Cuts at: 520 1356 2220 4020 4301 520
Size: 836 864 1800 281 1087
Fragments arranged by size:
1800 1087 864 836 281
AgeI A'CCGG_T
Cuts at: 1689 1689
Size: 4868
AleI CACnn'nnGTG
Cuts at: 121 121
Size: 4868
AlwNI CAG_nnn'CTG
Cuts at: 1155 2173 3611 1155
Size: 1018 1438 2412
Fragments arranged by size:
2412 1438 1018
ApaLI G'TGCA_C
Cuts at: 3706 3706
Size: 4868
ApoI r'AATT_y
Cuts at: 1606 1872 4221 4477 1606
Size: 266 2349 256 1997
Fragments arranged by size:
2349 1997 266 256
AseI AT'TA_AT
Cuts at: 20 1027 20
Size: 1007 3861
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
AvaI C'yCGr_G
Cuts at: 1888 1888
Size: 4868
BamHI G'GATC_C
Cuts at: 1893 1893
Size: 4868
BanI G'GyrC_C
Cuts at: 648 1719 1884 2298 2546 2581 648
Size: 1071 165 414 248 35 2935
Fragments arranged by size:
2935 1071 414 248 165 35
BanII G_rGCy'C
Cuts at: 1882 2912 1882
Size: 1030 3838
BbeI G_GCGC'C
Cuts at: 2302 2550 2302
Size: 248 4620
BbvCI CC'TCA_GC
Cuts at: 1019 1109 1019
Size: 90 4778
BciVI GTATCCnnnnn_n'
Cuts at: 2766 3822 4868 2766
Size: 1056 1046 2766
Fragments arranged by size:
2766 1056 1046
BclI T'GATC_A
Cuts at: 2388 2388
Size: 4868
BglII A'GATC_T
Cuts at: 2383 4420 2383
Size: 2037 2831
BlpI GC'TnA_GC
Cuts at: 1736 1980 1736
Size: 244 4624
Bme1580I G_kGCm'C
Cuts at: 785 2493 2586 3710 785
Size: 1708 93 1124 1943
Fragments arranged by size:
1943 1708 1124 93
BmgBI CAC'GTC
Cuts at: 1357 1357
Size: 4868
BmrI ACTGGGnnnn_n'
Cuts at: 781 2080 2161 2496 4640 781
Size: 1299 81 335 2144 1009
Fragments arranged by size:
2144 1299 1009 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3235 3235
Size: 4868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 365
Size: 4868
Bpu10I CC'TnA_GC
Cuts at: 1019 1109 1019
Size: 90 4778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1697 2044 2923 3637 3935 1697
Size: 347 879 714 298 2630
Fragments arranged by size:
2630 879 714 347 298
BsaI GGTCTCn'nnnn_
Cuts at: 126 341 1589 4572 126
Size: 215 1248 2983 422
Fragments arranged by size:
2983 1248 422 215
BsaAI yAC'GTr
Cuts at: 523 2221 523
Size: 1698 3170
BsaBI GATnn'nnATC
Cuts at: 6 1340 2382 4419 6
Size: 1334 1042 2037 455
Fragments arranged by size:
2037 1334 1042 455
BsaHI Gr'CG_yC
Cuts at: 2299 2547 3249 4745 2299
Size: 248 702 1496 2422
Fragments arranged by size:
2422 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1689 2057 2578 3256 3667 3814 4088 1689
Size: 368 521 678 411 147 274 2469
Fragments arranged by size:
2469 678 521 411 368 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 986 1166 1837 986
Size: 180 671 4017
Fragments arranged by size:
4017 671 180
BseYI C'CCAG_C
Cuts at: 1121 1166 2293 3051 3716 4124 1121
Size: 45 1127 758 665 408 1865
Fragments arranged by size:
1865 1127 758 665 408 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1156 818
Size: 338 4530
BsiEI CG_ry'CG
Cuts at: 104 2410 2456 3686 104
Size: 2306 46 1230 1286
Fragments arranged by size:
2306 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1882 2660 2850 3710 1882
Size: 778 190 860 3040
Fragments arranged by size:
3040 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 785 1882 2493 2586 2660 2850 2912 3710 785
Size: 1097 611 93 74 190 62 798 1943
Fragments arranged by size:
1943 1097 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1918 2132 2434 2815 1918
Size: 214 302 381 3971
Fragments arranged by size:
3971 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1216 3160 3214 4698 4863 50
Size: 1166 1944 54 1484 165 55
Fragments arranged by size:
1944 1484 1166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 636 764 827 882 2780 636
Size: 128 63 55 1898 2724
Fragments arranged by size:
2724 1898 128 63 55
BsrFI r'CCGG_y
Cuts at: 1689 2866 3047 1689
Size: 1177 181 3510
Fragments arranged by size:
3510 1177 181
BssSI C'ACGA_G
Cuts at: 3139 3847 3139
Size: 708 4160
BstAPI GCAn_nnn'nTGC
Cuts at: 1155 1287 2245 1155
Size: 132 958 3778
Fragments arranged by size:
3778 958 132
BstBI TT'CG_AA
Cuts at: 3229 3229
Size: 4868
BstEII G'GTnAC_C
Cuts at: 700 1692 700
Size: 992 3876
BstXI CCAn_nnnn'nTGG
Cuts at: 123 818 1210 123
Size: 695 392 3781
Fragments arranged by size:
3781 695 392
BstYI r'GATC_y
Cuts at: 1 267 526 1622 1893 2360 2383 2718 2964
Size: 266 259 1096 271 467 23 335 246
Cuts at: 2964 3270 3282 3368 4420 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1096 1052 467 449 335 306 271 266
259 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2818 2999 3162 2818
Size: 181 163 4524
Fragments arranged by size:
4524 181 163
BtsI GCAGTG_nn'
Cuts at: 722 756 801 1227 1521 2111 722
Size: 34 45 426 294 590 3479
Fragments arranged by size:
3479 590 426 294 45 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1025 1060 1025
Size: 35 4833
DraI TTT'AAA
Cuts at: 1867 1867
Size: 4868
DraIII CAC_nnn'GTG
Cuts at: 1134 1134
Size: 4868
DrdI GACnn_nn'nnGTC
Cuts at: 2574 3918 2574
Size: 1344 3524
EaeI y'GGCC_r
Cuts at: 2453 2627 3018 3045 4390 2453
Size: 174 391 27 1345 2931
Fragments arranged by size:
2931 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2453 2453
Size: 4868
EarI CTCTTCn'nnn_
Cuts at: 1504 1814 2891 3101 1504
Size: 310 1077 210 3271
Fragments arranged by size:
3271 1077 310 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3076 3818 3964 4678 3076
Size: 742 146 714 3266
Fragments arranged by size:
3266 742 714 146
EcoICRI GAG'CTC
Cuts at: 1880 1880
Size: 4868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 1366 1485 2693 3125 3478 4140 365
Size: 1001 119 1208 432 353 662 1093
Fragments arranged by size:
1208 1093 1001 662 432 353 119
EcoO109I rG'GnC_Cy
Cuts at: 1105 2007 1105
Size: 902 3966
EcoRI G'AATT_C
Cuts at: 1872 1872
Size: 4868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 572 2887 572
Size: 2315 2553
FspI TGC'GCA
Cuts at: 2649 2649
Size: 4868
HaeII r_GCGC'y
Cuts at: 2302 2550 3780 4551 2302
Size: 248 1230 771 2619
Fragments arranged by size:
2619 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2710 2740 2742 2772 2710
Size: 30 2 30 4806
Fragments arranged by size:
4806 30 30 2
HincII GTy'rAC
Cuts at: 430 469 1907 430
Size: 39 1438 3391
Fragments arranged by size:
3391 1438 39
KasI G'GCGC_C
Cuts at: 2298 2546 2298
Size: 248 4620
KpnI G_GTAC'C
Cuts at: 1723 1888 1723
Size: 165 4703
MluI A'CGCG_T
Cuts at: 4301 4301
Size: 4868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3627 3811 3627
Size: 184 4684
MscI TGG'CCA
Cuts at: 2629 2629
Size: 4868
MslI CAynn'nnrTG
Cuts at: 121 1526 2857 2984 121
Size: 1405 1331 127 2005
Fragments arranged by size:
2005 1405 1331 127
MspA1I CmG'CkG
Cuts at: 343 712 1155 1979 2293 2653 3437 3682 343
Size: 369 443 824 314 360 784 245 1529
Fragments arranged by size:
1529 824 784 443 369 360 314 245
NaeI GCC'GGC
Cuts at: 3049 3049
Size: 4868
NarI GG'CG_CC
Cuts at: 2299 2547 2299
Size: 248 4620
NgoMIV G'CCGG_C
Cuts at: 3047 3047
Size: 4868
NspI r_CATG'y
Cuts at: 279 1921 2421 4024 279
Size: 1642 500 1603 1123
Fragments arranged by size:
1642 1603 1123 500
PciI A'CATG_T
Cuts at: 4020 4020
Size: 4868
PflMI CCAn_nnn'nTGG
Cuts at: 301 463 301
Size: 162 4706
PmeI GTTT'AAAC
Cuts at: 1867 1867
Size: 4868
PmlI CAC'GTG
Cuts at: 523 523
Size: 4868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4836
PpuMI rG'GwC_Cy
Cuts at: 1105 1105
Size: 4868
PsiI TTA'TAA
Cuts at: 1366 1366
Size: 4868
PstI C_TGCA'G
Cuts at: 717 1160 1915 2600 717
Size: 443 755 685 2985
Fragments arranged by size:
2985 755 685 443
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4868
PvuII CAG'CTG
Cuts at: 343 712 1155 2293 2653 343
Size: 369 443 1138 360 2558
Fragments arranged by size:
2558 1138 443 369 360
RsrII CG'GwC_CG
Cuts at: 3063 3063
Size: 4868
SacI G_AGCT'C
Cuts at: 1882 1882
Size: 4868
SalI G'TCGA_C
Cuts at: 1905 1905
Size: 4868
SapI GCTCTTCn'nnn_
Cuts at: 2891 3101 2891
Size: 210 4658
SbfI CC_TGCA'GG
Cuts at: 1915 1915
Size: 4868
ScaI AGT'ACT
Cuts at: 1428 4385 1428
Size: 2957 1911
SfcI C'TryA_G
Cuts at: 33 713 728 1156 1911 2596 3564 3755 33
Size: 680 15 428 755 685 968 191 1146
Fragments arranged by size:
1146 968 755 685 680 428 191 15
SfoI GGC'GCC
Cuts at: 2300 2548 2300
Size: 248 4620
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
SmaI CCC'GGG
Cuts at: 1890 1890
Size: 4868
SmlI C'TyrA_G
Cuts at: 222 1712 2023 2938 3652 3914 222
Size: 1490 311 915 714 262 1176
Fragments arranged by size:
1490 1176 915 714 311 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4868
SphI G_CATG'C
Cuts at: 1921 2421 1921
Size: 500 4368
SspI AAT'ATT
Cuts at: 933 933
Size: 4868
StyI C'CwwG_G
Cuts at: 318 869 2002 2356 318
Size: 551 1133 354 2830
Fragments arranged by size:
2830 1133 551 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2451 2584 3043 3252 2451
Size: 133 459 209 4067
Fragments arranged by size:
4067 459 209 133
TatI w'GTAC_w
Cuts at: 162 1426 4383 162
Size: 1264 2957 647
Fragments arranged by size:
2957 1264 647
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 316 348 4385 4417 316
Size: 32 4037 32 767
Fragments arranged by size:
4037 767 32 32
Tth111I GACn'n_nGTC
Cuts at: 2665 2665
Size: 4868
XbaI T'CTAG_A
Cuts at: 1899 1899
Size: 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 2363 876
Size: 1487 3381
XmaI C'CCGG_G
Cuts at: 1888 1888
Size: 4868
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmtI BsaXI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstZ17I Bsu36I
BtgI ClaI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI