Restriction map for
pF1KB8699
(Circular) (Six-base) MAPSORT of: pF1KB8699.seq Check: 7751 from: 1 to: 4736
pF1KB8699 4736 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1774 1774
Size: 4736
Acc65I G'GTAC_C
Cuts at: 214 1752 214
Size: 1538 3198
AclI AA'CG_TT
Cuts at: 1450 4576 1450
Size: 3126 1610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 316 2561 2993 3346 4008 252
Size: 64 2245 432 353 662 980
Fragments arranged by size:
2245 980 662 432 353 64
AflII C'TTAA_G
Cuts at: 706 706
Size: 4736
AflIII A'CryG_T
Cuts at: 1120 2088 3888 4169 1120
Size: 968 1800 281 1687
Fragments arranged by size:
1800 1687 968 281
AlwNI CAG_nnn'CTG
Cuts at: 1633 2041 3479 1633
Size: 408 1438 2890
Fragments arranged by size:
2890 1438 408
ApaLI G'TGCA_C
Cuts at: 454 3574 454
Size: 3120 1616
ApoI r'AATT_y
Cuts at: 833 1314 1480 1558 1663 1740 4089 4345 833
Size: 481 166 78 105 77 2349 256 1224
Fragments arranged by size:
2349 1224 481 256 166 105 78 77
AseI AT'TA_AT
Cuts at: 20 20
Size: 4736
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
AvaI C'yCGr_G
Cuts at: 181 1756 181
Size: 1575 3161
BamHI G'GATC_C
Cuts at: 1761 1761
Size: 4736
BanI G'GyrC_C
Cuts at: 214 328 397 816 963 1752 2166 2414 2449
Size: 114 69 419 147 789 414 248 35
Cuts at: 2449 214
Size: 2501
Fragments arranged by size:
2501 789 419 414 248 147 114 69
35
BanII G_rGCy'C
Cuts at: 182 646 1750 2780 182
Size: 464 1104 1030 2138
Fragments arranged by size:
2138 1104 1030 464
BbeI G_GCGC'C
Cuts at: 332 967 2170 2418 332
Size: 635 1203 248 2650
Fragments arranged by size:
2650 1203 635 248
BbsI GAAGACnn'nnnn_
Cuts at: 459 1338 1712 459
Size: 879 374 3483
Fragments arranged by size:
3483 879 374
BciVI GTATCCnnnnn_n'
Cuts at: 2634 3690 4736 2634
Size: 1056 1046 2634
Fragments arranged by size:
2634 1056 1046
BclI T'GATC_A
Cuts at: 2256 2256
Size: 4736
BfrBI ATG'CAT
Cuts at: 850 850
Size: 4736
BglI GCCn_nnn'nGGC
Cuts at: 391 391
Size: 4736
BglII A'GATC_T
Cuts at: 2251 4288 2251
Size: 2037 2699
BlpI GC'TnA_GC
Cuts at: 1848 1848
Size: 4736
Bme1580I G_kGCm'C
Cuts at: 458 588 1128 2361 2454 3578 458
Size: 130 540 1233 93 1124 1616
Fragments arranged by size:
1616 1233 1124 540 130 93
BmrI ACTGGGnnnn_n'
Cuts at: 369 807 1948 2029 2364 4508 369
Size: 438 1141 81 335 2144 597
Fragments arranged by size:
2144 1141 597 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1255 3103 1255
Size: 1848 2888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 596 1565 196
Size: 400 969 3367
Fragments arranged by size:
3367 969 400
Bpu10I CC'TnA_GC
Cuts at: 632 632
Size: 4736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1912 2791 3505 3803 1912
Size: 879 714 298 2845
Fragments arranged by size:
2845 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4440 4440
Size: 4736
BsaAI yAC'GTr
Cuts at: 2089 2089
Size: 4736
BsaBI GATnn'nnATC
Cuts at: 6 2250 4287 6
Size: 2244 2037 455
Fragments arranged by size:
2244 2037 455
BsaHI Gr'CG_yC
Cuts at: 329 964 2167 2415 3117 4613 329
Size: 635 1203 248 702 1496 452
Fragments arranged by size:
1496 1203 702 635 452 248
BsaWI w'CCGG_w
Cuts at: 1925 2446 3124 3535 3682 3956 1925
Size: 521 678 411 147 274 2705
Fragments arranged by size:
2705 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 340
Size: 30 4706
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 303 483 498 1340 1343 1397 303
Size: 180 15 842 3 54 3642
Fragments arranged by size:
3642 842 180 54 15 3
BseYI C'CCAG_C
Cuts at: 151 812 2161 2919 3584 3992 151
Size: 661 1349 758 665 408 895
Fragments arranged by size:
1349 895 758 665 661 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 4736
BsiEI CG_ry'CG
Cuts at: 104 2278 2324 3554 104
Size: 2174 46 1230 1286
Fragments arranged by size:
2174 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 458 1750 2528 2718 3578 458
Size: 1292 778 190 860 1616
Fragments arranged by size:
1616 1292 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 182 458 588 646 1128 1750 2361 2454 2528
Size: 276 130 58 482 622 611 93 74
Cuts at: 2528 2718 2780 3578 182
Size: 190 62 798 1340
Fragments arranged by size:
1340 798 622 611 482 276 190 130
93 74 62 58
BspHI T'CATG_A
Cuts at: 1023 3168 1023
Size: 2145 2591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 1786 2000 2302 2683 179
Size: 1607 214 302 381 2232
Fragments arranged by size:
2232 1607 381 302 214
BsrBI CCG'CTC
Cuts at: 50 128 3028 3082 4566 4731 50
Size: 78 2900 54 1484 165 55
Fragments arranged by size:
2900 1484 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 2648 2648
Size: 4736
BsrFI r'CCGG_y
Cuts at: 284 394 2734 2915 284
Size: 110 2340 181 2105
Fragments arranged by size:
2340 2105 181 110
BssSI C'ACGA_G
Cuts at: 457 3007 3715 457
Size: 2550 708 1478
Fragments arranged by size:
2550 1478 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2113 2113
Size: 4736
BstBI TT'CG_AA
Cuts at: 1260 1329 3097 1260
Size: 69 1768 2899
Fragments arranged by size:
2899 1768 69
BstYI r'GATC_y
Cuts at: 1 122 1761 2228 2251 2586 2832 3138 3150
Size: 121 1639 467 23 335 246 306 12
Cuts at: 3150 3236 4288 1
Size: 86 1052 449
Fragments arranged by size:
1639 1052 467 449 335 306 246 121
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1488 1488
Size: 4736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2686 2867 3030 2686
Size: 181 163 4392
Fragments arranged by size:
4392 181 163
BtsI GCAGTG_nn'
Cuts at: 130 1080 1979 130
Size: 950 899 2887
Fragments arranged by size:
2887 950 899
DraI TTT'AAA
Cuts at: 1455 1735 1455
Size: 280 4456
DrdI GACnn_nn'nnGTC
Cuts at: 2442 3786 2442
Size: 1344 3392
EaeI y'GGCC_r
Cuts at: 392 2321 2495 2886 2913 4258 392
Size: 1929 174 391 27 1345 870
Fragments arranged by size:
1929 1345 870 391 174 27
EagI C'GGCC_G
Cuts at: 2321 2321
Size: 4736
EarI CTCTTCn'nnn_
Cuts at: 1046 1158 1224 1241 2759 2969 1046
Size: 112 66 17 1518 210 2813
Fragments arranged by size:
2813 1518 210 112 66 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 667 2944 3686 3832 4546 667
Size: 2277 742 146 714 857
Fragments arranged by size:
2277 857 742 714 146
EcoICRI GAG'CTC
Cuts at: 1748 1748
Size: 4736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 252 316 596 1565 2561 2993 3346 4008
Size: 56 64 280 969 996 432 353 662
Cuts at: 4008 196
Size: 924
Fragments arranged by size:
996 969 924 662 432 353 280 64
56
EcoO109I rG'GnC_Cy
Cuts at: 168 1875 168
Size: 1707 3029
EcoRI G'AATT_C
Cuts at: 833 1740 833
Size: 907 3829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4736
FspI TGC'GCA
Cuts at: 2517 2517
Size: 4736
HaeII r_GCGC'y
Cuts at: 332 967 2170 2418 3648 4419 332
Size: 635 1203 248 1230 771 649
Fragments arranged by size:
1230 1203 771 649 635 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 675 707 1069 1101 2578 2608 2610 2640 675
Size: 32 362 32 1477 30 2 30 2771
Fragments arranged by size:
2771 1477 362 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1775 1775
Size: 4736
HindIII A'AGCT_T
Cuts at: 251 703 939 1549 1573 251
Size: 452 236 610 24 3414
Fragments arranged by size:
3414 610 452 236 24
KasI G'GCGC_C
Cuts at: 328 963 2166 2414 328
Size: 635 1203 248 2650
Fragments arranged by size:
2650 1203 635 248
KpnI G_GTAC'C
Cuts at: 218 1756 218
Size: 1538 3198
MluI A'CGCG_T
Cuts at: 1120 4169 1120
Size: 3049 1687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3495 3679 3495
Size: 184 4552
MscI TGG'CCA
Cuts at: 2497 2497
Size: 4736
MslI CAynn'nnrTG
Cuts at: 1241 2725 2852 1241
Size: 1484 127 3125
Fragments arranged by size:
3125 1484 127
MspA1I CmG'CkG
Cuts at: 294 345 1847 2161 2521 3305 3550 294
Size: 51 1502 314 360 784 245 1480
Fragments arranged by size:
1502 1480 784 360 314 245 51
NaeI GCC'GGC
Cuts at: 396 2917 396
Size: 2521 2215
NarI GG'CG_CC
Cuts at: 329 964 2167 2415 329
Size: 635 1203 248 2650
Fragments arranged by size:
2650 1203 635 248
NgoMIV G'CCGG_C
Cuts at: 394 2915 394
Size: 2521 2215
NsiI A_TGCA'T
Cuts at: 852 852
Size: 4736
NspI r_CATG'y
Cuts at: 854 1789 2289 3892 854
Size: 935 500 1603 1698
Fragments arranged by size:
1698 1603 935 500
PciI A'CATG_T
Cuts at: 3888 3888
Size: 4736
PmeI GTTT'AAAC
Cuts at: 1735 1735
Size: 4736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1547 1579 3182 3214 1547
Size: 32 1603 32 3069
Fragments arranged by size:
3069 1603 32 32
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 4736
PstI C_TGCA'G
Cuts at: 1783 2468 1783
Size: 685 4051
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4736
PvuII CAG'CTG
Cuts at: 294 2161 2521 294
Size: 1867 360 2509
Fragments arranged by size:
2509 1867 360
RsrII CG'GwC_CG
Cuts at: 2931 2931
Size: 4736
SacI G_AGCT'C
Cuts at: 1750 1750
Size: 4736
SalI G'TCGA_C
Cuts at: 1773 1773
Size: 4736
SapI GCTCTTCn'nnn_
Cuts at: 2759 2969 2759
Size: 210 4526
SbfI CC_TGCA'GG
Cuts at: 1783 1783
Size: 4736
ScaI AGT'ACT
Cuts at: 4253 4253
Size: 4736
SexAI A'CCwGG_T
Cuts at: 1193 1193
Size: 4736
SfcI C'TryA_G
Cuts at: 33 289 1779 2464 3432 3623 33
Size: 256 1490 685 968 191 1146
Fragments arranged by size:
1490 1146 968 685 256 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 391 391
Size: 4736
SfoI GGC'GCC
Cuts at: 330 965 2168 2416 330
Size: 635 1203 248 2650
Fragments arranged by size:
2650 1203 635 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
SmaI CCC'GGG
Cuts at: 183 1758 183
Size: 1575 3161
SmlI C'TyrA_G
Cuts at: 706 1891 2806 3520 3782 706
Size: 1185 915 714 262 1660
Fragments arranged by size:
1660 1185 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4736
SphI G_CATG'C
Cuts at: 1789 2289 1789
Size: 500 4236
StyI C'CwwG_G
Cuts at: 1870 2224 1870
Size: 354 4382
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 804 2319 2452 2911 3120 804
Size: 1515 133 459 209 2420
Fragments arranged by size:
2420 1515 459 209 133
TatI w'GTAC_w
Cuts at: 4251 4251
Size: 4736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 4253 4285 340
Size: 32 3881 32 791
Fragments arranged by size:
3881 791 32 32
Tth111I GACn'n_nGTC
Cuts at: 2533 2533
Size: 4736
XbaI T'CTAG_A
Cuts at: 786 1767 786
Size: 981 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2231
Size: 4736
XmaI C'CCGG_G
Cuts at: 181 1756 181
Size: 1575 3161
XmnI GAAnn'nnTTC
Cuts at: 1562 1562
Size: 4736
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI