Restriction map for
pF1KB8704
(Circular) (Six-base) MAPSORT of: pF1KB8704.seq Check: 3855 from: 1 to: 3827
pF1KB8704 3827 bp
With 182 enzymes: SgfI *
July 15, 2008 09:38 ..
AccI GT'mk_AC
Cuts at: 865 865
Size: 3827
Acc65I G'GTAC_C
Cuts at: 843 843
Size: 3827
AclI AA'CG_TT
Cuts at: 3667 3667
Size: 3827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 596 743 1652 2084 2437 3099 320
Size: 276 147 909 432 353 662 1048
Fragments arranged by size:
1048 909 662 432 353 276 147
AflIII A'CryG_T
Cuts at: 1179 2979 3260 1179
Size: 1800 281 1746
Fragments arranged by size:
1800 1746 281
AlwNI CAG_nnn'CTG
Cuts at: 388 1132 2570 388
Size: 744 1438 1645
Fragments arranged by size:
1645 1438 744
ApaLI G'TGCA_C
Cuts at: 2665 2665
Size: 3827
ApoI r'AATT_y
Cuts at: 218 831 3180 3436 218
Size: 613 2349 256 609
Fragments arranged by size:
2349 613 609 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3827
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
AvaI C'yCGr_G
Cuts at: 847 847
Size: 3827
BamHI G'GATC_C
Cuts at: 852 852
Size: 3827
BanI G'GyrC_C
Cuts at: 358 843 1257 1505 1540 358
Size: 485 414 248 35 2645
Fragments arranged by size:
2645 485 414 248 35
BanII G_rGCy'C
Cuts at: 410 841 1871 410
Size: 431 1030 2366
Fragments arranged by size:
2366 1030 431
BbeI G_GCGC'C
Cuts at: 1261 1509 1261
Size: 248 3579
BbvCI CC'TCA_GC
Cuts at: 293 293
Size: 3827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 601 635 90
Size: 34 477 34 3282
Fragments arranged by size:
3282 477 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1725 2781 3827 1725
Size: 1056 1046 1725
Fragments arranged by size:
1725 1056 1046
BclI T'GATC_A
Cuts at: 1347 1347
Size: 3827
BglI GCCn_nnn'nGGC
Cuts at: 484 484
Size: 3827
BglII A'GATC_T
Cuts at: 1342 3379 1342
Size: 2037 1790
BlpI GC'TnA_GC
Cuts at: 516 939 516
Size: 423 3404
Bme1580I G_kGCm'C
Cuts at: 771 1452 1545 2669 771
Size: 681 93 1124 1929
Fragments arranged by size:
1929 1124 681 93
BmrI ACTGGGnnnn_n'
Cuts at: 713 1039 1120 1455 3599 713
Size: 326 81 335 2144 941
Fragments arranged by size:
2144 941 335 326 81
BmtI G_CTAG'C
Cuts at: 230 230
Size: 3827
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 3827
Bpu10I CC'TnA_GC
Cuts at: 293 293
Size: 3827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1882 2596 2894 1003
Size: 879 714 298 1936
Fragments arranged by size:
1936 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3531 3531
Size: 3827
BsaAI yAC'GTr
Cuts at: 1180 1180
Size: 3827
BsaBI GATnn'nnATC
Cuts at: 6 1341 3378 6
Size: 1335 2037 455
Fragments arranged by size:
2037 1335 455
BsaHI Gr'CG_yC
Cuts at: 1258 1506 2208 3704 1258
Size: 248 702 1496 1381
Fragments arranged by size:
1496 1381 702 248
BsaWI w'CCGG_w
Cuts at: 1016 1537 2215 2626 2773 3047 1016
Size: 521 678 411 147 274 1796
Fragments arranged by size:
1796 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 517 610 451
Size: 66 93 3668
Fragments arranged by size:
3668 93 66
BseYI C'CCAG_C
Cuts at: 381 585 686 1252 2010 2675 3083 381
Size: 204 101 566 758 665 408 1125
Fragments arranged by size:
1125 758 665 566 408 204 101
BsiEI CG_ry'CG
Cuts at: 104 1369 1415 2645 104
Size: 1265 46 1230 1286
Fragments arranged by size:
1286 1265 1230 46
BsiHKAI G_wGCw'C
Cuts at: 841 1619 1809 2669 841
Size: 778 190 860 1999
Fragments arranged by size:
1999 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 410 771 841 1452 1545 1619 1809 1871 2669
Size: 361 70 611 93 74 190 62 798
Cuts at: 2669 410
Size: 1568
Fragments arranged by size:
1568 798 611 361 190 93 74 70
62
BspHI T'CATG_A
Cuts at: 2259 2259
Size: 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 877 1091 1393 1774 877
Size: 214 302 381 2930
Fragments arranged by size:
2930 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2119 2173 3657 3822 50
Size: 2069 54 1484 165 55
Fragments arranged by size:
2069 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1739 1739
Size: 3827
BsrFI r'CCGG_y
Cuts at: 511 1825 2006 511
Size: 1314 181 2332
Fragments arranged by size:
2332 1314 181
BssSI C'ACGA_G
Cuts at: 2098 2806 2098
Size: 708 3119
BstAPI GCAn_nnn'nTGC
Cuts at: 664 1204 664
Size: 540 3287
BstBI TT'CG_AA
Cuts at: 2188 2188
Size: 3827
BstYI r'GATC_y
Cuts at: 1 852 1319 1342 1677 1923 2229 2241 2327
Size: 851 467 23 335 246 306 12 86
Cuts at: 2327 3379 1
Size: 1052 449
Fragments arranged by size:
1052 851 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 758 800 758
Size: 42 3785
BtgI C'CryG_G
Cuts at: 234 234
Size: 3827
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1777 1958 2121 1777
Size: 181 163 3483
Fragments arranged by size:
3483 181 163
BtsI GCAGTG_nn'
Cuts at: 629 1070 629
Size: 441 3386
DraI TTT'AAA
Cuts at: 826 826
Size: 3827
DrdI GACnn_nn'nnGTC
Cuts at: 1533 2877 1533
Size: 1344 2483
EaeI y'GGCC_r
Cuts at: 1412 1586 1977 2004 3349 1412
Size: 174 391 27 1345 1890
Fragments arranged by size:
1890 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1412 1412
Size: 3827
EarI CTCTTCn'nnn_
Cuts at: 719 785 1850 2060 719
Size: 66 1065 210 2486
Fragments arranged by size:
2486 1065 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2035 2777 2923 3637 2035
Size: 742 146 714 2225
Fragments arranged by size:
2225 742 714 146
EcoICRI GAG'CTC
Cuts at: 839 839
Size: 3827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 596 743 1652 2084 2437 3099 320
Size: 276 147 909 432 353 662 1048
Fragments arranged by size:
1048 909 662 432 353 276 147
EcoO109I rG'GnC_Cy
Cuts at: 966 966
Size: 3827
EcoRI G'AATT_C
Cuts at: 831 831
Size: 3827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1846 1846
Size: 3827
FspI TGC'GCA
Cuts at: 1608 1608
Size: 3827
HaeII r_GCGC'y
Cuts at: 1261 1509 2739 3510 1261
Size: 248 1230 771 1578
Fragments arranged by size:
1578 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1669 1699 1701 1731 1669
Size: 30 2 30 3765
Fragments arranged by size:
3765 30 30 2
HincII GTy'rAC
Cuts at: 866 866
Size: 3827
HindIII A'AGCT_T
Cuts at: 542 542
Size: 3827
KasI G'GCGC_C
Cuts at: 1257 1505 1257
Size: 248 3579
KpnI G_GTAC'C
Cuts at: 847 847
Size: 3827
MluI A'CGCG_T
Cuts at: 3260 3260
Size: 3827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 718 2586 2770 718
Size: 1868 184 1775
Fragments arranged by size:
1868 1775 184
MscI TGG'CCA
Cuts at: 1588 1588
Size: 3827
MslI CAynn'nnrTG
Cuts at: 1816 1943 1816
Size: 127 3700
MspA1I CmG'CkG
Cuts at: 520 938 1252 1612 2396 2641 520
Size: 418 314 360 784 245 1706
Fragments arranged by size:
1706 784 418 360 314 245
NaeI GCC'GGC
Cuts at: 513 2008 513
Size: 1495 2332
NarI GG'CG_CC
Cuts at: 1258 1506 1258
Size: 248 3579
NgoMIV G'CCGG_C
Cuts at: 511 2006 511
Size: 1495 2332
NheI G'CTAG_C
Cuts at: 226 226
Size: 3827
NspI r_CATG'y
Cuts at: 820 880 1380 2983 820
Size: 60 500 1603 1664
Fragments arranged by size:
1664 1603 500 60
PciI A'CATG_T
Cuts at: 2979 2979
Size: 3827
PflMI CCAn_nnn'nTGG
Cuts at: 388 388
Size: 3827
PmeI GTTT'AAAC
Cuts at: 826 826
Size: 3827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2273 2305 2273
Size: 32 3795
PstI C_TGCA'G
Cuts at: 874 1559 874
Size: 685 3142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3827
PvuII CAG'CTG
Cuts at: 520 1252 1612 520
Size: 732 360 2735
Fragments arranged by size:
2735 732 360
RsrII CG'GwC_CG
Cuts at: 2022 2022
Size: 3827
SacI G_AGCT'C
Cuts at: 841 841
Size: 3827
SalI G'TCGA_C
Cuts at: 864 864
Size: 3827
SapI GCTCTTCn'nnn_
Cuts at: 1850 2060 1850
Size: 210 3617
SbfI CC_TGCA'GG
Cuts at: 874 874
Size: 3827
ScaI AGT'ACT
Cuts at: 3344 3344
Size: 3827
SfcI C'TryA_G
Cuts at: 33 740 870 1555 2523 2714 33
Size: 707 130 685 968 191 1146
Fragments arranged by size:
1146 968 707 685 191 130
SfoI GGC'GCC
Cuts at: 1259 1507 1259
Size: 248 3579
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
SmaI CCC'GGG
Cuts at: 849 849
Size: 3827
SmlI C'TyrA_G
Cuts at: 982 1897 2611 2873 982
Size: 915 714 262 1936
Fragments arranged by size:
1936 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3827
SphI G_CATG'C
Cuts at: 880 1380 880
Size: 500 3327
StyI C'CwwG_G
Cuts at: 569 590 674 961 1315 569
Size: 21 84 287 354 3081
Fragments arranged by size:
3081 354 287 84 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1410 1543 2002 2211 1410
Size: 133 459 209 3026
Fragments arranged by size:
3026 459 209 133
TatI w'GTAC_w
Cuts at: 3342 3342
Size: 3827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 3795
Tth111I GACn'n_nGTC
Cuts at: 1624 1624
Size: 3827
XbaI T'CTAG_A
Cuts at: 858 858
Size: 3827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1322 1322
Size: 3827
XmaI C'CCGG_G
Cuts at: 847 847
Size: 3827
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BfrBI BmgBI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI