Restriction map for
pF1KB8705
(Circular) (Six-base) MAPSORT of: pF1KB8705.seq Check: 5183 from: 1 to: 4241
pF1KB8705 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 2851 3513 2066
Size: 432 353 662 2794
Fragments arranged by size:
2794 662 432 353
AflIII A'CryG_T
Cuts at: 317 1593 3393 3674 317
Size: 1276 1800 281 884
Fragments arranged by size:
1800 1276 884 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1080 1112 1080
Size: 32 4209
AlwNI CAG_nnn'CTG
Cuts at: 361 1546 2984 361
Size: 1185 1438 1618
Fragments arranged by size:
1618 1438 1185
ApaLI G'TGCA_C
Cuts at: 3079 3079
Size: 4241
ApoI r'AATT_y
Cuts at: 885 1245 3594 3850 885
Size: 360 2349 256 1276
Fragments arranged by size:
2349 1276 360 256
AseI AT'TA_AT
Cuts at: 20 985 1126 20
Size: 965 141 3135
Fragments arranged by size:
3135 965 141
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 174 1261 174
Size: 1087 3154
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1033 1066 1033
Size: 33 4208
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 1257 1671 1919 1954 1257
Size: 414 248 35 3544
Fragments arranged by size:
3544 414 248 35
BanII G_rGCy'C
Cuts at: 1255 2285 1255
Size: 1030 3211
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 316 350 316
Size: 34 4207
BciVI GTATCCnnnnn_n'
Cuts at: 2139 3195 4241 2139
Size: 1056 1046 2139
Fragments arranged by size:
2139 1056 1046
BclI T'GATC_A
Cuts at: 726 1761 726
Size: 1035 3206
BglI GCCn_nnn'nGGC
Cuts at: 343 343
Size: 4241
BglII A'GATC_T
Cuts at: 170 1756 3793 170
Size: 1586 2037 618
Fragments arranged by size:
2037 1586 618
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 1866 1959 3083 1866
Size: 93 1124 3024
Fragments arranged by size:
3024 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1016 2608 1016
Size: 1592 2649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 491 1214 300
Size: 191 723 3327
Fragments arranged by size:
3327 723 191
Bpu10I CC'TnA_GC
Cuts at: 291 291
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 3010 3308 1417
Size: 879 714 298 2350
Fragments arranged by size:
2350 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3945 3945
Size: 4241
BsaAI yAC'GTr
Cuts at: 1212 1594 1212
Size: 382 3859
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 3040 3187 3461 1430
Size: 521 678 411 147 274 2210
Fragments arranged by size:
2210 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 989 989
Size: 4241
BseYI C'CCAG_C
Cuts at: 793 1666 2424 3089 3497 793
Size: 873 758 665 408 1537
Fragments arranged by size:
1537 873 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 698
Size: 4241
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3059 104
Size: 1679 46 1230 1286
Fragments arranged by size:
1679 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 654 1255 2033 2223 3083 654
Size: 601 778 190 860 1812
Fragments arranged by size:
1812 860 778 601 190
Bsp1286I G_dGCh'C
Cuts at: 654 1255 1866 1959 2033 2223 2285 3083 654
Size: 601 611 93 74 190 62 798 1812
Fragments arranged by size:
1812 798 611 601 190 93 74 62
BspHI T'CATG_A
Cuts at: 759 875 2673 759
Size: 116 1798 2327
Fragments arranged by size:
2327 1798 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1505 1807 2188 1291
Size: 214 302 381 3344
Fragments arranged by size:
3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 415 2533 2587 4071 4236 50
Size: 365 2118 54 1484 165 55
Fragments arranged by size:
2118 1484 365 165 55 54
BsrDI GCAATG_nn'
Cuts at: 681 2153 681
Size: 1472 2769
BsrFI r'CCGG_y
Cuts at: 2239 2420 2239
Size: 181 4060
BssSI C'ACGA_G
Cuts at: 2512 3220 2512
Size: 708 3533
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1618 1141
Size: 477 3764
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 170 906 1266 1733 1756 2091 2337 2643
Size: 169 736 360 467 23 335 246 306
Cuts at: 2643 2655 2741 3793 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 736 467 449 360 335 306 246
169 86 23 12
BtgI C'CryG_G
Cuts at: 621 1232 621
Size: 611 3630
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 332 458 1484 332
Size: 126 1026 3089
Fragments arranged by size:
3089 1026 126
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 3291 1947
Size: 1344 2897
EaeI y'GGCC_r
Cuts at: 618 669 1826 2000 2391 2418 3763 618
Size: 51 1157 174 391 27 1345 1096
Fragments arranged by size:
1345 1157 1096 391 174 51 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 3191 3337 4051 2449
Size: 742 146 714 2639
Fragments arranged by size:
2639 742 714 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 491 1214 2066 2498 2851 3513 300
Size: 191 723 852 432 353 662 1028
Fragments arranged by size:
1028 852 723 662 432 353 191
EcoO109I rG'GnC_Cy
Cuts at: 790 1115 1380 790
Size: 325 265 3651
Fragments arranged by size:
3651 325 265
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 3153 3924 1675
Size: 248 1230 771 1992
Fragments arranged by size:
1992 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 754 786 891 923 2083 2113 2115
Size: 32 343 32 105 32 1160 30 2
Cuts at: 2115 2145 379
Size: 30 2475
Fragments arranged by size:
2475 1160 343 105 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 329 957 3000 3184 224
Size: 105 628 2043 184 1281
Fragments arranged by size:
2043 1281 628 184 105
MscI TGG'CCA
Cuts at: 620 671 2002 620
Size: 51 1331 2859
Fragments arranged by size:
2859 1331 51
MslI CAynn'nnrTG
Cuts at: 608 671 692 722 728 734 740 758 877
Size: 63 21 30 6 6 6 18 119
Cuts at: 877 2230 2357 608
Size: 1353 127 2492
Fragments arranged by size:
2492 1353 127 119 63 30 21 18
6 6 6
MspA1I CmG'CkG
Cuts at: 1352 1666 2026 2810 3055 1352
Size: 314 360 784 245 2538
Fragments arranged by size:
2538 784 360 314 245
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 1232 1232
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 321 1294 1794 3397 321
Size: 973 500 1603 1165
Fragments arranged by size:
1603 1165 973 500
PciI A'CATG_T
Cuts at: 317 3393 317
Size: 3076 1165
PfoI T'CCnGG_A
Cuts at: 211 211
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 404 436 2687 2719 404
Size: 32 2251 32 1926
Fragments arranged by size:
2251 1926 32 32
PpuMI rG'GwC_Cy
Cuts at: 790 1115 790
Size: 325 3916
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 1666 2026 1666
Size: 360 3881
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 1284 1969 2937 3128 33
Size: 1251 685 968 191 1146
Fragments arranged by size:
1251 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 1396 2311 3025 3287 1396
Size: 915 714 262 2350
Fragments arranged by size:
2350 915 714 262
SnaBI TAC'GTA
Cuts at: 1212 1212
Size: 4241
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
SspI AAT'ATT
Cuts at: 575 983 1179 575
Size: 408 196 3637
Fragments arranged by size:
3637 408 196
StyI C'CwwG_G
Cuts at: 1232 1375 1729 1232
Size: 143 354 3744
Fragments arranged by size:
3744 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 162 1824 1957 2416 2625 162
Size: 1662 133 459 209 1778
Fragments arranged by size:
1778 1662 459 209 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 3758 3790 619
Size: 32 3107 32 1070
Fragments arranged by size:
3107 1070 32 32
Tth111I GACn'n_nGTC
Cuts at: 629 2038 629
Size: 1409 2832
XbaI T'CTAG_A
Cuts at: 654 1272 654
Size: 618 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BbsI BbvCI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI XmnI ZraI