Restriction map for
pF1KB8706
(Circular) (Six-base) MAPSORT of: pF1KB8706.seq Check: 9505 from: 1 to: 4583
pF1KB8706 4583 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 947 2408 2840 3193 3855 596
Size: 351 1461 432 353 662 1324
Fragments arranged by size:
1461 1324 662 432 353 351
AflII C'TTAA_G
Cuts at: 931 931
Size: 4583
AflIII A'CryG_T
Cuts at: 1935 3735 4016 1935
Size: 1800 281 2502
Fragments arranged by size:
2502 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 126 126
Size: 4583
AlwNI CAG_nnn'CTG
Cuts at: 1888 3326 1888
Size: 1438 3145
ApaLI G'TGCA_C
Cuts at: 3421 3421
Size: 4583
ApoI r'AATT_y
Cuts at: 815 978 1386 1453 1587 3936 4192 815
Size: 163 408 67 134 2349 256 1206
Fragments arranged by size:
2349 1206 408 256 163 134 67
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 1603 1603
Size: 4583
AvrII C'CTAG_G
Cuts at: 1491 1491
Size: 4583
BamHI G'GATC_C
Cuts at: 1608 1608
Size: 4583
BanI G'GyrC_C
Cuts at: 1599 2013 2261 2296 1599
Size: 414 248 35 3886
Fragments arranged by size:
3886 414 248 35
BanII G_rGCy'C
Cuts at: 543 1303 1597 2627 543
Size: 760 294 1030 2499
Fragments arranged by size:
2499 1030 760 294
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbsI GAAGACnn'nnnn_
Cuts at: 806 863 806
Size: 57 4526
BciVI GTATCCnnnnn_n'
Cuts at: 998 2481 3537 4583 998
Size: 1483 1056 1046 998
Fragments arranged by size:
1483 1056 1046 998
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BglII A'GATC_T
Cuts at: 2098 4135 2098
Size: 2037 2546
BlpI GC'TnA_GC
Cuts at: 1695 1695
Size: 4583
Bme1580I G_kGCm'C
Cuts at: 2208 2301 3425 2208
Size: 93 1124 3366
Fragments arranged by size:
3366 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1339 1795 1876 2211 4355 1339
Size: 456 81 335 2144 1567
Fragments arranged by size:
2144 1567 456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1322 2950 1322
Size: 1628 2955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 832
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2638 3352 3650 1759
Size: 879 714 298 2692
Fragments arranged by size:
2692 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 2014 2262 2964 4460 2014
Size: 248 702 1496 2137
Fragments arranged by size:
2137 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1772 2293 2971 3382 3529 3803 1772
Size: 521 678 411 147 274 2552
Fragments arranged by size:
2552 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 322 1380 2008 2766 3431 3839 322
Size: 1058 628 758 665 408 1066
Fragments arranged by size:
1066 1058 758 665 628 408
BsiEI CG_ry'CG
Cuts at: 104 2125 2171 3401 104
Size: 2021 46 1230 1286
Fragments arranged by size:
2021 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 301 1303 1597 2375 2565 3425 301
Size: 1002 294 778 190 860 1459
Fragments arranged by size:
1459 1002 860 778 294 190
BsmBI CGTCTCn'nnnn_
Cuts at: 846 846
Size: 4583
Bsp1286I G_dGCh'C
Cuts at: 301 543 1303 1597 2208 2301 2375 2565 2627
Size: 242 760 294 611 93 74 190 62
Cuts at: 2627 3425 301
Size: 798 1459
Fragments arranged by size:
1459 798 760 611 294 242 190 93
74 62
BspHI T'CATG_A
Cuts at: 3015 3015
Size: 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1633 1847 2149 2530 1633
Size: 214 302 381 3686
Fragments arranged by size:
3686 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2875 2929 4413 4578 50
Size: 2825 54 1484 165 55
Fragments arranged by size:
2825 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 366 1088 2495 366
Size: 722 1407 2454
Fragments arranged by size:
2454 1407 722
BsrFI r'CCGG_y
Cuts at: 2581 2762 2581
Size: 181 4402
BsrGI T'GTAC_A
Cuts at: 730 730
Size: 4583
BssSI C'ACGA_G
Cuts at: 2854 3562 2854
Size: 708 3875
BstAPI GCAn_nnn'nTGC
Cuts at: 1960 1960
Size: 4583
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstYI r'GATC_y
Cuts at: 1 1608 2075 2098 2433 2679 2985 2997 3083
Size: 1607 467 23 335 246 306 12 86
Cuts at: 3083 4135 1
Size: 1052 449
Fragments arranged by size:
1607 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4583
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2533 2714 2877 2533
Size: 181 163 4239
Fragments arranged by size:
4239 181 163
BtsI GCAGTG_nn'
Cuts at: 320 1826 320
Size: 1506 3077
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3633 2289
Size: 1344 3239
EaeI y'GGCC_r
Cuts at: 2168 2342 2733 2760 4105 2168
Size: 174 391 27 1345 2646
Fragments arranged by size:
2646 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2791 3533 3679 4393 2791
Size: 742 146 714 2981
Fragments arranged by size:
2981 742 714 146
EcoICRI GAG'CTC
Cuts at: 1301 1595 1301
Size: 294 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 832 947 2408 2840 3193 3855 596
Size: 236 115 1461 432 353 662 1324
Fragments arranged by size:
1461 1324 662 432 353 236 115
EcoNI CCTnn'n_nnAGG
Cuts at: 526 1109 526
Size: 583 4000
EcoO109I rG'GnC_Cy
Cuts at: 1102 1488 1722 1102
Size: 386 234 3963
Fragments arranged by size:
3963 386 234
EcoRI G'AATT_C
Cuts at: 1587 1587
Size: 4583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4583
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 2017 2265 3495 4266 2017
Size: 248 1230 771 2334
Fragments arranged by size:
2334 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 1161 1193 2425 2455 2457 2487 112
Size: 32 1017 32 1232 30 2 30 2208
Fragments arranged by size:
2208 1232 1017 32 32 30 30 2
HincII GTy'rAC
Cuts at: 276 1622 276
Size: 1346 3237
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 881 3342 3526 479
Size: 402 2461 184 1536
Fragments arranged by size:
2461 1536 402 184
MscI TGG'CCA
Cuts at: 2344 2344
Size: 4583
MslI CAynn'nnrTG
Cuts at: 763 1255 2572 2699 763
Size: 492 1317 127 2647
Fragments arranged by size:
2647 1317 492 127
MspA1I CmG'CkG
Cuts at: 322 994 1694 2008 2368 3152 3397 322
Size: 672 700 314 360 784 245 1508
Fragments arranged by size:
1508 784 700 672 360 314 245
NaeI GCC'GGC
Cuts at: 2764 2764
Size: 4583
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NcoI C'CATG_G
Cuts at: 107 107
Size: 4583
NgoMIV G'CCGG_C
Cuts at: 2762 2762
Size: 4583
NspI r_CATG'y
Cuts at: 1636 2136 3739 1636
Size: 500 1603 2480
Fragments arranged by size:
2480 1603 500
PciI A'CATG_T
Cuts at: 3735 3735
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 4551
PshAI GACnn'nnGTC
Cuts at: 559 559
Size: 4583
PstI C_TGCA'G
Cuts at: 1467 1630 2315 1467
Size: 163 685 3735
Fragments arranged by size:
3735 685 163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 322 2008 2368 322
Size: 1686 360 2537
Fragments arranged by size:
2537 1686 360
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1303 1597 1303
Size: 294 4289
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 1206 1463 1626 2311 3279 3470 33
Size: 1173 257 163 685 968 191 1146
Fragments arranged by size:
1173 1146 968 685 257 191 163
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 1605 1605
Size: 4583
SmlI C'TyrA_G
Cuts at: 931 1738 2653 3367 3629 931
Size: 807 915 714 262 1885
Fragments arranged by size:
1885 915 807 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
SspI AAT'ATT
Cuts at: 1053 1053
Size: 4583
StyI C'CwwG_G
Cuts at: 107 1491 1717 2071 107
Size: 1384 226 354 2619
Fragments arranged by size:
2619 1384 354 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2166 2299 2758 2967 2166
Size: 133 459 209 3782
Fragments arranged by size:
3782 459 209 133
TatI w'GTAC_w
Cuts at: 730 4098 730
Size: 3368 1215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1265 1297 4100 4132 1265
Size: 32 2803 32 1716
Fragments arranged by size:
2803 1716 32 32
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2078 2078
Size: 4583
XmaI C'CCGG_G
Cuts at: 1603 1603
Size: 4583
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BspEI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI