Restriction map for
pF1KB8707
(Circular) (Six-base) MAPSORT of: pF1KB8707.seq Check: 9993 from: 1 to: 4730
pF1KB8707 4730 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 1629 1768 1629
Size: 139 4591
Acc65I G'GTAC_C
Cuts at: 1746 1746
Size: 4730
AclI AA'CG_TT
Cuts at: 4570 4570
Size: 4730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 2555 2987 3340 4002 1673
Size: 882 432 353 662 2401
Fragments arranged by size:
2401 882 662 432 353
AflII C'TTAA_G
Cuts at: 657 657
Size: 4730
AflIII A'CryG_T
Cuts at: 2082 3882 4163 2082
Size: 1800 281 2649
Fragments arranged by size:
2649 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1579 2035 3473 1579
Size: 456 1438 2836
Fragments arranged by size:
2836 1438 456
ApaLI G'TGCA_C
Cuts at: 3568 3568
Size: 4730
ApoI r'AATT_y
Cuts at: 376 581 772 946 1311 1734 4083 4339 376
Size: 205 191 174 365 423 2349 256 767
Fragments arranged by size:
2349 767 423 365 256 205 191 174
AseI AT'TA_AT
Cuts at: 20 20
Size: 4730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
AvaI C'yCGr_G
Cuts at: 1750 1750
Size: 4730
AvrII C'CTAG_G
Cuts at: 973 973
Size: 4730
BamHI G'GATC_C
Cuts at: 1755 1755
Size: 4730
BanI G'GyrC_C
Cuts at: 1746 2160 2408 2443 1746
Size: 414 248 35 4033
Fragments arranged by size:
4033 414 248 35
BanII G_rGCy'C
Cuts at: 1744 2774 1744
Size: 1030 3700
BbeI G_GCGC'C
Cuts at: 2164 2412 2164
Size: 248 4482
BbsI GAAGACnn'nnnn_
Cuts at: 188 1100 188
Size: 912 3818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 678 712 678
Size: 34 4696
BciVI GTATCCnnnnn_n'
Cuts at: 2628 3684 4730 2628
Size: 1056 1046 2628
Fragments arranged by size:
2628 1056 1046
BclI T'GATC_A
Cuts at: 2250 2250
Size: 4730
BfrBI ATG'CAT
Cuts at: 669 669
Size: 4730
BglII A'GATC_T
Cuts at: 2245 4282 2245
Size: 2037 2693
BlpI GC'TnA_GC
Cuts at: 1842 1842
Size: 4730
Bme1580I G_kGCm'C
Cuts at: 2355 2448 3572 2355
Size: 93 1124 3513
Fragments arranged by size:
3513 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1942 2023 2358 4502 1942
Size: 81 335 2144 2170
Fragments arranged by size:
2170 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 4730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 872
Size: 4730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1906 2785 3499 3797 1906
Size: 879 714 298 2839
Fragments arranged by size:
2839 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1628 4434 1628
Size: 2806 1924
BsaAI yAC'GTr
Cuts at: 1483 2083 1483
Size: 600 4130
BsaBI GATnn'nnATC
Cuts at: 6 2244 4281 6
Size: 2238 2037 455
Fragments arranged by size:
2238 2037 455
BsaHI Gr'CG_yC
Cuts at: 1428 2161 2409 3111 4607 1428
Size: 733 248 702 1496 1551
Fragments arranged by size:
1551 1496 733 702 248
BsaWI w'CCGG_w
Cuts at: 1297 1919 2440 3118 3529 3676 3950 1297
Size: 622 521 678 411 147 274 2077
Fragments arranged by size:
2077 678 622 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 284 314 284
Size: 30 4700
BseYI C'CCAG_C
Cuts at: 2155 2913 3578 3986 2155
Size: 758 665 408 2899
Fragments arranged by size:
2899 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 1168
Size: 4730
BsiEI CG_ry'CG
Cuts at: 104 2272 2318 3548 104
Size: 2168 46 1230 1286
Fragments arranged by size:
2168 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1514 1744 2522 2712 3572 1514
Size: 230 778 190 860 2672
Fragments arranged by size:
2672 860 778 230 190
BsiWI C'GTAC_G
Cuts at: 685 685
Size: 4730
BsmI GAATG_Cn'
Cuts at: 742 742
Size: 4730
BsmBI CGTCTCn'nnnn_
Cuts at: 1419 1419
Size: 4730
Bsp1286I G_dGCh'C
Cuts at: 1514 1744 2355 2448 2522 2712 2774 3572 1514
Size: 230 611 93 74 190 62 798 2672
Fragments arranged by size:
2672 798 611 230 190 93 74 62
BspHI T'CATG_A
Cuts at: 177 765 3162 177
Size: 588 2397 1745
Fragments arranged by size:
2397 1745 588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1140 1780 1994 2296 2677 1140
Size: 640 214 302 381 3193
Fragments arranged by size:
3193 640 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3022 3076 4560 4725 50
Size: 2972 54 1484 165 55
Fragments arranged by size:
2972 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2642 2642
Size: 4730
BsrFI r'CCGG_y
Cuts at: 2728 2909 2728
Size: 181 4549
BssSI C'ACGA_G
Cuts at: 3001 3709 3001
Size: 708 4022
BstAPI GCAn_nnn'nTGC
Cuts at: 2107 2107
Size: 4730
BstBI TT'CG_AA
Cuts at: 3091 3091
Size: 4730
BstXI CCAn_nnnn'nTGG
Cuts at: 341 341
Size: 4730
BstYI r'GATC_y
Cuts at: 1 1518 1755 2222 2245 2580 2826 3132 3144
Size: 1517 237 467 23 335 246 306 12
Cuts at: 3144 3230 4282 1
Size: 86 1052 449
Fragments arranged by size:
1517 1052 467 449 335 306 246 237
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 509 509
Size: 4730
BtgI C'CryG_G
Cuts at: 107 107
Size: 4730
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2680 2861 3024 2680
Size: 181 163 4386
Fragments arranged by size:
4386 181 163
BtsI GCAGTG_nn'
Cuts at: 1973 1973
Size: 4730
DraI TTT'AAA
Cuts at: 489 1729 489
Size: 1240 3490
DraIII CAC_nnn'GTG
Cuts at: 1486 1486
Size: 4730
DrdI GACnn_nn'nnGTC
Cuts at: 2436 3780 2436
Size: 1344 3386
EaeI y'GGCC_r
Cuts at: 2315 2489 2880 2907 4252 2315
Size: 174 391 27 1345 2793
Fragments arranged by size:
2793 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2315 2315
Size: 4730
EarI CTCTTCn'nnn_
Cuts at: 637 710 1257 1434 2753 2963 637
Size: 73 547 177 1319 210 2404
Fragments arranged by size:
2404 1319 547 210 177 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2938 3680 3826 4540 127
Size: 2811 742 146 714 317
Fragments arranged by size:
2811 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 1742 1742
Size: 4730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 1673 2555 2987 3340 4002 872
Size: 801 882 432 353 662 1600
Fragments arranged by size:
1600 882 801 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1282 1869 1282
Size: 587 4143
EcoRI G'AATT_C
Cuts at: 1734 1734
Size: 4730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 205 2749 205
Size: 2544 2186
FspI TGC'GCA
Cuts at: 2511 2511
Size: 4730
HaeII r_GCGC'y
Cuts at: 2164 2412 3642 4413 2164
Size: 248 1230 771 2481
Fragments arranged by size:
2481 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 638 670 2572 2602 2604 2634 638
Size: 32 1902 30 2 30 2734
Fragments arranged by size:
2734 1902 32 30 30 2
HincII GTy'rAC
Cuts at: 1769 1769
Size: 4730
KasI G'GCGC_C
Cuts at: 2160 2408 2160
Size: 248 4482
KpnI G_GTAC'C
Cuts at: 1750 1750
Size: 4730
MluI A'CGCG_T
Cuts at: 4163 4163
Size: 4730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 966 3489 3673 966
Size: 2523 184 2023
Fragments arranged by size:
2523 2023 184
MscI TGG'CCA
Cuts at: 2491 2491
Size: 4730
MslI CAynn'nnrTG
Cuts at: 339 1412 2719 2846 339
Size: 1073 1307 127 2223
Fragments arranged by size:
2223 1307 1073 127
MspA1I CmG'CkG
Cuts at: 1230 1841 2155 2515 3299 3544 1230
Size: 611 314 360 784 245 2416
Fragments arranged by size:
2416 784 611 360 314 245
NaeI GCC'GGC
Cuts at: 2911 2911
Size: 4730
NarI GG'CG_CC
Cuts at: 2161 2409 2161
Size: 248 4482
NcoI C'CATG_G
Cuts at: 107 107
Size: 4730
NgoMIV G'CCGG_C
Cuts at: 2909 2909
Size: 4730
NsiI A_TGCA'T
Cuts at: 671 671
Size: 4730
NspI r_CATG'y
Cuts at: 578 669 1783 2283 3886 578
Size: 91 1114 500 1603 1422
Fragments arranged by size:
1603 1422 1114 500 91
PciI A'CATG_T
Cuts at: 3882 3882
Size: 4730
PflMI CCAn_nnn'nTGG
Cuts at: 1486 1486
Size: 4730
PfoI T'CCnGG_A
Cuts at: 849 849
Size: 4730
PmeI GTTT'AAAC
Cuts at: 1729 1729
Size: 4730
PmlI CAC'GTG
Cuts at: 1483 1483
Size: 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4698
PpuMI rG'GwC_Cy
Cuts at: 1282 1282
Size: 4730
PstI C_TGCA'G
Cuts at: 1575 1777 2462 1575
Size: 202 685 3843
Fragments arranged by size:
3843 685 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4730
PvuII CAG'CTG
Cuts at: 1230 2155 2515 1230
Size: 925 360 3445
Fragments arranged by size:
3445 925 360
RsrII CG'GwC_CG
Cuts at: 2925 2925
Size: 4730
SacI G_AGCT'C
Cuts at: 1744 1744
Size: 4730
SalI G'TCGA_C
Cuts at: 1767 1767
Size: 4730
SapI GCTCTTCn'nnn_
Cuts at: 2753 2963 2753
Size: 210 4520
SbfI CC_TGCA'GG
Cuts at: 1777 1777
Size: 4730
ScaI AGT'ACT
Cuts at: 1605 4247 1605
Size: 2642 2088
SfcI C'TryA_G
Cuts at: 33 1213 1571 1773 2458 3426 3617 33
Size: 1180 358 202 685 968 191 1146
Fragments arranged by size:
1180 1146 968 685 358 202 191
SfoI GGC'GCC
Cuts at: 2162 2410 2162
Size: 248 4482
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
SmaI CCC'GGG
Cuts at: 1752 1752
Size: 4730
SmlI C'TyrA_G
Cuts at: 657 1885 2800 3514 3776 657
Size: 1228 915 714 262 1611
Fragments arranged by size:
1611 1228 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4730
SphI G_CATG'C
Cuts at: 578 1783 2283 578
Size: 1205 500 3025
Fragments arranged by size:
3025 1205 500
StyI C'CwwG_G
Cuts at: 107 973 1864 2218 107
Size: 866 891 354 2619
Fragments arranged by size:
2619 891 866 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 329 2313 2446 2905 3114 329
Size: 1984 133 459 209 1945
Fragments arranged by size:
1984 1945 459 209 133
TatI w'GTAC_w
Cuts at: 1603 4245 1603
Size: 2642 2088
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4247 4279 4247
Size: 32 4698
Tth111I GACn'n_nGTC
Cuts at: 2527 2527
Size: 4730
XbaI T'CTAG_A
Cuts at: 1158 1761 1158
Size: 603 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 334 341 2225 334
Size: 7 1884 2839
Fragments arranged by size:
2839 1884 7
XmaI C'CCGG_G
Cuts at: 1750 1750
Size: 4730
XmnI GAAnn'nnTTC
Cuts at: 440 1452 440
Size: 1012 3718
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BglI BmgBI BmtI Bpu10I
BseRI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI