Restriction map for
pF1KB8709
(Circular) (Six-base) MAPSORT of: pF1KB8709.seq Check: 2725 from: 1 to: 4328
pF1KB8709 4328 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 449 449
Size: 4328
AatII G_ACGT'C
Cuts at: 1222 1222
Size: 4328
AccI GT'mk_AC
Cuts at: 656 1366 656
Size: 710 3618
Acc65I G'GTAC_C
Cuts at: 1344 1344
Size: 4328
AclI AA'CG_TT
Cuts at: 236 4168 236
Size: 3932 396
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 234 1147 2153 2585 2938 3600 212
Size: 22 913 1006 432 353 662 940
Fragments arranged by size:
1006 940 913 662 432 353 22
AflIII A'CryG_T
Cuts at: 1680 3480 3761 1680
Size: 1800 281 2247
Fragments arranged by size:
2247 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 384 441 1633 3071 384
Size: 57 1192 1438 1641
Fragments arranged by size:
1641 1438 1192 57
ApaI G_GGCC'C
Cuts at: 369 369
Size: 4328
ApaLI G'TGCA_C
Cuts at: 3166 3166
Size: 4328
ApoI r'AATT_y
Cuts at: 582 1095 1332 3681 3937 582
Size: 513 237 2349 256 973
Fragments arranged by size:
2349 973 513 256 237
AseI AT'TA_AT
Cuts at: 20 20
Size: 4328
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4328
AvaI C'yCGr_G
Cuts at: 1348 1348
Size: 4328
BamHI G'GATC_C
Cuts at: 177 1353 177
Size: 1176 3152
BanI G'GyrC_C
Cuts at: 346 798 1344 1758 2006 2041 346
Size: 452 546 414 248 35 2633
Fragments arranged by size:
2633 546 452 414 248 35
BanII G_rGCy'C
Cuts at: 369 851 1342 2372 369
Size: 482 491 1030 2325
Fragments arranged by size:
2325 1030 491 482
BbeI G_GCGC'C
Cuts at: 1762 2010 1762
Size: 248 4080
BbsI GAAGACnn'nnnn_
Cuts at: 124 888 124
Size: 764 3564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4294
BciVI GTATCCnnnnn_n'
Cuts at: 2226 3282 4328 2226
Size: 1056 1046 2226
Fragments arranged by size:
2226 1056 1046
BclI T'GATC_A
Cuts at: 1848 1848
Size: 4328
BglII A'GATC_T
Cuts at: 1843 3880 1843
Size: 2037 2291
BlpI GC'TnA_GC
Cuts at: 1440 1440
Size: 4328
Bme1580I G_kGCm'C
Cuts at: 349 369 803 1953 2046 3170 349
Size: 20 434 1150 93 1124 1507
Fragments arranged by size:
1507 1150 1124 434 93 20
BmrI ACTGGGnnnn_n'
Cuts at: 603 1208 1312 1540 1621 1956 4100 603
Size: 605 104 228 81 335 2144 831
Fragments arranged by size:
2144 831 605 335 228 104 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 552 2695 552
Size: 2143 2185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 538 547 1049 1075 1305 361
Size: 177 9 502 26 230 3384
Fragments arranged by size:
3384 502 230 177 26 9
Bpu10I CC'TnA_GC
Cuts at: 327 327
Size: 4328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 1504 2383 3097 3395 297
Size: 1207 879 714 298 1230
Fragments arranged by size:
1230 1207 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4032 4032
Size: 4328
BsaAI yAC'GTr
Cuts at: 879 1681 879
Size: 802 3526
BsaBI GATnn'nnATC
Cuts at: 6 1842 3879 6
Size: 1836 2037 455
Fragments arranged by size:
2037 1836 455
BsaHI Gr'CG_yC
Cuts at: 1118 1219 1759 2007 2709 4205 1118
Size: 101 540 248 702 1496 1241
Fragments arranged by size:
1496 1241 702 540 248 101
BsaWI w'CCGG_w
Cuts at: 909 1517 2038 2716 3127 3274 3548 909
Size: 608 521 678 411 147 274 1689
Fragments arranged by size:
1689 678 608 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 106 775 1186 106
Size: 669 411 3248
Fragments arranged by size:
3248 669 411
BseYI C'CCAG_C
Cuts at: 368 681 1121 1753 2511 3176 3584 368
Size: 313 440 632 758 665 408 1112
Fragments arranged by size:
1112 758 665 632 440 408 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 886 926 427
Size: 459 40 3829
Fragments arranged by size:
3829 459 40
BsiEI CG_ry'CG
Cuts at: 104 1870 1916 3146 104
Size: 1766 46 1230 1286
Fragments arranged by size:
1766 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 823 851 1342 2120 2310 3170 823
Size: 28 491 778 190 860 1981
Fragments arranged by size:
1981 860 778 491 190 28
Bsp1286I G_dGCh'C
Cuts at: 349 369 803 823 851 1342 1953 2046 2120
Size: 20 434 20 28 491 611 93 74
Cuts at: 2120 2310 2372 3170 349
Size: 190 62 798 1507
Fragments arranged by size:
1507 798 611 491 434 190 93 74
62 28 20 20
BspEI T'CCGG_A
Cuts at: 909 909
Size: 4328
BspHI T'CATG_A
Cuts at: 837 1262 2760 837
Size: 425 1498 2405
Fragments arranged by size:
2405 1498 425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 1378 1592 1894 2275 449
Size: 929 214 302 381 2502
Fragments arranged by size:
2502 929 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2620 2674 4158 4323 50
Size: 2570 54 1484 165 55
Fragments arranged by size:
2570 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 498 891 1067 2240 498
Size: 393 176 1173 2586
Fragments arranged by size:
2586 1173 393 176
BsrFI r'CCGG_y
Cuts at: 2326 2507 2326
Size: 181 4147
BsrGI T'GTAC_A
Cuts at: 122 1004 122
Size: 882 3446
BssSI C'ACGA_G
Cuts at: 2599 3307 2599
Size: 708 3620
BstAPI GCAn_nnn'nTGC
Cuts at: 1705 1705
Size: 4328
BstBI TT'CG_AA
Cuts at: 2689 2689
Size: 4328
BstEII G'GTnAC_C
Cuts at: 1017 1203 1017
Size: 186 4142
BstYI r'GATC_y
Cuts at: 1 177 419 1353 1820 1843 2178 2424 2730
Size: 176 242 934 467 23 335 246 306
Cuts at: 2730 2742 2828 3880 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 934 467 449 335 306 246 242
176 86 23 12
BtgI C'CryG_G
Cuts at: 173 320 485 173
Size: 147 165 4016
Fragments arranged by size:
4016 165 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 921 2278 2459 2622 921
Size: 1357 181 163 2627
Fragments arranged by size:
2627 1357 181 163
BtsI GCAGTG_nn'
Cuts at: 1571 1571
Size: 4328
DraI TTT'AAA
Cuts at: 1327 1327
Size: 4328
DraIII CAC_nnn'GTG
Cuts at: 270 270
Size: 4328
DrdI GACnn_nn'nnGTC
Cuts at: 2034 3378 2034
Size: 1344 2984
EaeI y'GGCC_r
Cuts at: 354 1913 2087 2478 2505 3850 354
Size: 1559 174 391 27 1345 832
Fragments arranged by size:
1559 1345 832 391 174 27
EagI C'GGCC_G
Cuts at: 1913 1913
Size: 4328
EarI CTCTTCn'nnn_
Cuts at: 2351 2561 2351
Size: 210 4118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2536 3278 3424 4138 2536
Size: 742 146 714 2726
Fragments arranged by size:
2726 742 714 146
EcoICRI GAG'CTC
Cuts at: 849 1340 849
Size: 491 3837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 234 361 538 547 1049 1075 1147 1305
Size: 22 127 177 9 502 26 72 158
Cuts at: 1305 2153 2585 2938 3600 212
Size: 848 432 353 662 940
Fragments arranged by size:
940 848 662 502 432 353 177 158
127 72 26 22 9
EcoO109I rG'GnC_Cy
Cuts at: 219 381 1467 219
Size: 162 1086 3080
Fragments arranged by size:
3080 1086 162
EcoRI G'AATT_C
Cuts at: 1332 1332
Size: 4328
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 4328
FspI TGC'GCA
Cuts at: 2109 2109
Size: 4328
HaeII r_GCGC'y
Cuts at: 1762 2010 3240 4011 1762
Size: 248 1230 771 2079
Fragments arranged by size:
2079 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 2170 2200 2202 2232 195
Size: 32 1943 30 2 30 2291
Fragments arranged by size:
2291 1943 32 30 30 2
HincII GTy'rAC
Cuts at: 240 657 702 1367 240
Size: 417 45 665 3201
Fragments arranged by size:
3201 665 417 45
KasI G'GCGC_C
Cuts at: 1758 2006 1758
Size: 248 4080
KpnI G_GTAC'C
Cuts at: 1348 1348
Size: 4328
MluI A'CGCG_T
Cuts at: 3761 3761
Size: 4328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3087 3271 3087
Size: 184 4144
MscI TGG'CCA
Cuts at: 356 2089 356
Size: 1733 2595
MslI CAynn'nnrTG
Cuts at: 319 557 1261 2317 2444 319
Size: 238 704 1056 127 2203
Fragments arranged by size:
2203 1056 704 238 127
MspA1I CmG'CkG
Cuts at: 152 861 1125 1189 1439 1753 2113 2897 3142
Size: 709 264 64 250 314 360 784 245
Cuts at: 3142 152
Size: 1338
Fragments arranged by size:
1338 784 709 360 314 264 250 245
64
NaeI GCC'GGC
Cuts at: 2509 2509
Size: 4328
NarI GG'CG_CC
Cuts at: 1759 2007 1759
Size: 248 4080
NcoI C'CATG_G
Cuts at: 173 320 485 173
Size: 147 165 4016
Fragments arranged by size:
4016 165 147
NgoMIV G'CCGG_C
Cuts at: 2507 2507
Size: 4328
NspI r_CATG'y
Cuts at: 1381 1881 3484 1381
Size: 500 1603 2225
Fragments arranged by size:
2225 1603 500
PasI CC'CwG_GG
Cuts at: 342 342
Size: 4328
PciI A'CATG_T
Cuts at: 3480 3480
Size: 4328
PflMI CCAn_nnn'nTGG
Cuts at: 174 384 174
Size: 210 4118
PfoI T'CCnGG_A
Cuts at: 587 587
Size: 4328
PmeI GTTT'AAAC
Cuts at: 1327 1327
Size: 4328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2774 2806 2774
Size: 32 4296
PspOMI G'GGCC_C
Cuts at: 365 365
Size: 4328
PstI C_TGCA'G
Cuts at: 1375 2060 1375
Size: 685 3643
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4328
PvuII CAG'CTG
Cuts at: 861 1125 1189 1753 2113 861
Size: 264 64 564 360 3076
Fragments arranged by size:
3076 564 360 264 64
RsrII CG'GwC_CG
Cuts at: 2523 2523
Size: 4328
SacI G_AGCT'C
Cuts at: 851 1342 851
Size: 491 3837
SalI G'TCGA_C
Cuts at: 655 1365 655
Size: 710 3618
SapI GCTCTTCn'nnn_
Cuts at: 2351 2561 2351
Size: 210 4118
SbfI CC_TGCA'GG
Cuts at: 1375 1375
Size: 4328
ScaI AGT'ACT
Cuts at: 3845 3845
Size: 4328
SfcI C'TryA_G
Cuts at: 33 1371 2056 3024 3215 33
Size: 1338 685 968 191 1146
Fragments arranged by size:
1338 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1760 2008 1760
Size: 248 4080
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4328
SmaI CCC'GGG
Cuts at: 1350 1350
Size: 4328
SmlI C'TyrA_G
Cuts at: 276 1483 2398 3112 3374 276
Size: 1207 915 714 262 1230
Fragments arranged by size:
1230 1207 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4328
SphI G_CATG'C
Cuts at: 1381 1881 1381
Size: 500 3828
StuI AGG'CCT
Cuts at: 980 980
Size: 4328
StyI C'CwwG_G
Cuts at: 173 320 485 1462 1816 173
Size: 147 165 977 354 2685
Fragments arranged by size:
2685 977 354 165 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1126 1911 2044 2503 2712 1126
Size: 785 133 459 209 2742
Fragments arranged by size:
2742 785 459 209 133
TatI w'GTAC_w
Cuts at: 122 1004 3843 122
Size: 882 2839 607
Fragments arranged by size:
2839 882 607
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 4296
Tth111I GACn'n_nGTC
Cuts at: 2125 2125
Size: 4328
XbaI T'CTAG_A
Cuts at: 1359 1359
Size: 4328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 993 1823 693
Size: 300 830 3198
Fragments arranged by size:
3198 830 300
XmaI C'CCGG_G
Cuts at: 1348 1348
Size: 4328
XmnI GAAnn'nnTTC
Cuts at: 295 295
Size: 4328
ZraI GAC'GTC
Cuts at: 1220 1220
Size: 4328
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI