Restriction map for
pF1KB8714
(Circular) (Six-base) MAPSORT of: pF1KB8714.seq Check: 8039 from: 1 to: 4442
pF1KB8714 4442 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1480 1480
Size: 4442
Acc65I G'GTAC_C
Cuts at: 1458 1458
Size: 4442
AclI AA'CG_TT
Cuts at: 4282 4282
Size: 4442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 574 2267 2699 3052 3714 256
Size: 318 1693 432 353 662 984
Fragments arranged by size:
1693 984 662 432 353 318
AflIII A'CryG_T
Cuts at: 1794 3594 3875 1794
Size: 1800 281 2361
Fragments arranged by size:
2361 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 4442
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 202
Size: 32 4410
AlwNI CAG_nnn'CTG
Cuts at: 212 895 1747 3185 212
Size: 683 852 1438 1469
Fragments arranged by size:
1469 1438 852 683
ApaI G_GGCC'C
Cuts at: 725 760 796 887 1016 1339 725
Size: 35 36 91 129 323 3828
Fragments arranged by size:
3828 323 129 91 36 35
ApaLI G'TGCA_C
Cuts at: 3280 3280
Size: 4442
ApoI r'AATT_y
Cuts at: 1446 3795 4051 1446
Size: 2349 256 1837
Fragments arranged by size:
2349 1837 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
AvaI C'yCGr_G
Cuts at: 142 156 718 1462 142
Size: 14 562 744 3122
Fragments arranged by size:
3122 744 562 14
BamHI G'GATC_C
Cuts at: 1467 1467
Size: 4442
BanI G'GyrC_C
Cuts at: 262 829 1351 1458 1872 2120 2155 262
Size: 567 522 107 414 248 35 2549
Fragments arranged by size:
2549 567 522 414 248 107 35
BanII G_rGCy'C
Cuts at: 149 455 725 760 796 808 887 1016 1307
Size: 306 270 35 36 12 79 129 291
Cuts at: 1307 1339 1456 2486 149
Size: 32 117 1030 2105
Fragments arranged by size:
2105 1030 306 291 270 129 117 79
36 35 32 12
BbeI G_GCGC'C
Cuts at: 1876 2124 1876
Size: 248 4194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 409 443 409
Size: 34 4408
BciVI GTATCCnnnnn_n'
Cuts at: 2340 3396 4442 2340
Size: 1056 1046 2340
Fragments arranged by size:
2340 1056 1046
BclI T'GATC_A
Cuts at: 1962 1962
Size: 4442
BglI GCCn_nnn'nGGC
Cuts at: 864 1163 1343 864
Size: 299 180 3963
Fragments arranged by size:
3963 299 180
BglII A'GATC_T
Cuts at: 1957 3994 1957
Size: 2037 2405
BlpI GC'TnA_GC
Cuts at: 1554 1554
Size: 4442
Bme1580I G_kGCm'C
Cuts at: 404 725 760 796 832 887 1016 1339 2067
Size: 321 35 36 36 55 129 323 728
Cuts at: 2067 2160 3284 404
Size: 93 1124 1562
Fragments arranged by size:
1562 1124 728 323 321 129 93 55
36 36 35
BmrI ACTGGGnnnn_n'
Cuts at: 1001 1233 1654 1735 2070 4214 1001
Size: 232 421 81 335 2144 1229
Fragments arranged by size:
2144 1229 421 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 866 2809 866
Size: 1943 2499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 673 839 857 983 992 583
Size: 90 166 18 126 9 4033
Fragments arranged by size:
4033 166 126 90 18 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 2497 3211 3509 1618
Size: 879 714 298 2551
Fragments arranged by size:
2551 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 333 926 955 1196 4146 333
Size: 593 29 241 2950 629
Fragments arranged by size:
2950 629 593 241 29
BsaAI yAC'GTr
Cuts at: 1795 1795
Size: 4442
BsaBI GATnn'nnATC
Cuts at: 6 1956 3993 6
Size: 1950 2037 455
Fragments arranged by size:
2037 1950 455
BsaHI Gr'CG_yC
Cuts at: 1873 2121 2823 4319 1873
Size: 248 702 1496 1996
Fragments arranged by size:
1996 1496 702 248
BsaWI w'CCGG_w
Cuts at: 199 1400 1631 2152 2830 3241 3388 3662 199
Size: 1201 231 521 678 411 147 274 979
Fragments arranged by size:
1201 979 678 521 411 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 442 472 981 1011 1050 1080 202
Size: 30 210 30 509 30 39 30 3564
Fragments arranged by size:
3564 509 210 39 30 30 30 30
BseYI C'CCAG_C
Cuts at: 888 923 1171 1320 1357 1867 2625 3290 3698
Size: 35 248 149 37 510 758 665 408
Cuts at: 3698 888
Size: 1632
Fragments arranged by size:
1632 758 665 510 408 248 149 37
35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 4442
BsiEI CG_ry'CG
Cuts at: 104 1984 2030 3260 104
Size: 1880 46 1230 1286
Fragments arranged by size:
1880 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 220 1456 2234 2424 3284 220
Size: 1236 778 190 860 1378
Fragments arranged by size:
1378 1236 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 149 220 404 455 725 760 796 808 832
Size: 71 184 51 270 35 36 12 24
Cuts at: 832 887 1016 1307 1339 1456 2067 2160 2234
Size: 55 129 291 32 117 611 93 74
Cuts at: 2234 2424 2486 3284 149
Size: 190 62 798 1307
Fragments arranged by size:
1307 798 611 291 270 190 184 129
117 93 74 71 62 55 51 36
35 32 24 12
BspEI T'CCGG_A
Cuts at: 199 199
Size: 4442
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1492 1706 2008 2389 1492
Size: 214 302 381 3545
Fragments arranged by size:
3545 381 302 214
BsrBI CCG'CTC
Cuts at: 50 549 642 2734 2788 4272 4437 50
Size: 499 93 2092 54 1484 165 55
Fragments arranged by size:
2092 1484 499 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 937 2354 937
Size: 1417 3025
BsrFI r'CCGG_y
Cuts at: 727 745 1342 2440 2621 727
Size: 18 597 1098 181 2548
Fragments arranged by size:
2548 1098 597 181 18
BsrGI T'GTAC_A
Cuts at: 359 359
Size: 4442
BssSI C'ACGA_G
Cuts at: 2713 3421 2713
Size: 708 3734
BstAPI GCAn_nnn'nTGC
Cuts at: 1819 1819
Size: 4442
BstBI TT'CG_AA
Cuts at: 2803 2803
Size: 4442
BstEII G'GTnAC_C
Cuts at: 228 228
Size: 4442
BstYI r'GATC_y
Cuts at: 1 917 1467 1934 1957 2292 2538 2844 2856
Size: 916 550 467 23 335 246 306 12
Cuts at: 2856 2942 3994 1
Size: 86 1052 449
Fragments arranged by size:
1052 916 550 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 626 1196 626
Size: 570 3872
BtgI C'CryG_G
Cuts at: 107 118 1016 107
Size: 11 898 3533
Fragments arranged by size:
3533 898 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2392 2573 2736 2392
Size: 181 163 4098
Fragments arranged by size:
4098 181 163
BtsI GCAGTG_nn'
Cuts at: 1685 1685
Size: 4442
DraI TTT'AAA
Cuts at: 1441 1441
Size: 4442
DrdI GACnn_nn'nnGTC
Cuts at: 2148 3492 2148
Size: 1344 3098
EaeI y'GGCC_r
Cuts at: 810 1110 2027 2201 2592 2619 3964 810
Size: 300 917 174 391 27 1345 1288
Fragments arranged by size:
1345 1288 917 391 300 174 27
EagI C'GGCC_G
Cuts at: 2027 2027
Size: 4442
EarI CTCTTCn'nnn_
Cuts at: 1210 2465 2675 1210
Size: 1255 210 2977
Fragments arranged by size:
2977 1255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 495 1397 2650 3392 3538 4252 495
Size: 902 1253 742 146 714 685
Fragments arranged by size:
1253 902 742 714 685 146
EcoICRI GAG'CTC
Cuts at: 1454 1454
Size: 4442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 574 583 673 839 857 983 992 2267
Size: 318 9 90 166 18 126 9 1275
Cuts at: 2267 2699 3052 3714 256
Size: 432 353 662 984
Fragments arranged by size:
1275 984 662 432 353 318 166 126
90 18 9 9
EcoO109I rG'GnC_Cy
Cuts at: 630 649 756 792 793 847 865 884 985
Size: 19 107 36 1 54 18 19 101
Cuts at: 985 1003 1012 1021 1075 1084 1336 1581 630
Size: 18 9 9 54 9 252 245 3491
Fragments arranged by size:
3491 252 245 107 101 54 54 36
19 19 18 18 9 9 9 1
EcoRI G'AATT_C
Cuts at: 1446 1446
Size: 4442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4442
FspI TGC'GCA
Cuts at: 2223 2223
Size: 4442
HaeII r_GCGC'y
Cuts at: 133 170 490 1876 2124 3354 4125 133
Size: 37 320 1386 248 1230 771 450
Fragments arranged by size:
1386 1230 771 450 320 248 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1050 1082 1309 1341 2284 2314 2316 2346 1050
Size: 32 227 32 943 30 2 30 3146
Fragments arranged by size:
3146 943 227 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1481 1481
Size: 4442
KasI G'GCGC_C
Cuts at: 1872 2120 1872
Size: 248 4194
KpnI G_GTAC'C
Cuts at: 1462 1462
Size: 4442
MluI A'CGCG_T
Cuts at: 3875 3875
Size: 4442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 634 971 3201 3385 634
Size: 337 2230 184 1691
Fragments arranged by size:
2230 1691 337 184
MscI TGG'CCA
Cuts at: 1112 2203 1112
Size: 1091 3351
MslI CAynn'nnrTG
Cuts at: 2431 2558 2431
Size: 127 4315
MspA1I CmG'CkG
Cuts at: 120 1179 1553 1867 2227 3011 3256 120
Size: 1059 374 314 360 784 245 1306
Fragments arranged by size:
1306 1059 784 374 360 314 245
NaeI GCC'GGC
Cuts at: 747 1344 2623 747
Size: 597 1279 2566
Fragments arranged by size:
2566 1279 597
NarI GG'CG_CC
Cuts at: 1873 2121 1873
Size: 248 4194
NcoI C'CATG_G
Cuts at: 107 1016 107
Size: 909 3533
NgoMIV G'CCGG_C
Cuts at: 745 1342 2621 745
Size: 597 1279 2566
Fragments arranged by size:
2566 1279 597
NspI r_CATG'y
Cuts at: 1390 1495 1995 3598 1390
Size: 105 500 1603 2234
Fragments arranged by size:
2234 1603 500 105
PasI CC'CwG_GG
Cuts at: 397 663 799 880 981 1027 1054 397
Size: 266 136 81 101 46 27 3785
Fragments arranged by size:
3785 266 136 101 81 46 27
PciI A'CATG_T
Cuts at: 3594 3594
Size: 4442
PfoI T'CCnGG_A
Cuts at: 689 689
Size: 4442
PmeI GTTT'AAAC
Cuts at: 1441 1441
Size: 4442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 202 234 2888 2920 202
Size: 32 2654 32 1724
Fragments arranged by size:
2654 1724 32 32
PpuMI rG'GwC_Cy
Cuts at: 630 649 847 985 1021 1075 630
Size: 19 198 138 36 54 3997
Fragments arranged by size:
3997 198 138 54 36 19
PspOMI G'GGCC_C
Cuts at: 721 756 792 883 1012 1335 721
Size: 35 36 91 129 323 3828
Fragments arranged by size:
3828 323 129 91 36 35
PstI C_TGCA'G
Cuts at: 287 526 1489 2174 287
Size: 239 963 685 2555
Fragments arranged by size:
2555 963 685 239
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4442
PvuII CAG'CTG
Cuts at: 1867 2227 1867
Size: 360 4082
RsrII CG'GwC_CG
Cuts at: 2637 2637
Size: 4442
SacI G_AGCT'C
Cuts at: 1456 1456
Size: 4442
SacII CC_GC'GG
Cuts at: 121 121
Size: 4442
SalI G'TCGA_C
Cuts at: 1479 1479
Size: 4442
SanDI GG'GwC_CC
Cuts at: 649 985 1021 649
Size: 336 36 4070
Fragments arranged by size:
4070 336 36
SapI GCTCTTCn'nnn_
Cuts at: 1210 2465 2675 1210
Size: 1255 210 2977
Fragments arranged by size:
2977 1255 210
SbfI CC_TGCA'GG
Cuts at: 1489 1489
Size: 4442
ScaI AGT'ACT
Cuts at: 3959 3959
Size: 4442
SexAI A'CCwGG_T
Cuts at: 780 780
Size: 4442
SfcI C'TryA_G
Cuts at: 33 283 324 522 1485 2170 3138 3329 33
Size: 250 41 198 963 685 968 191 1146
Fragments arranged by size:
1146 968 963 685 250 198 191 41
SfiI GGCCn_nnn'nGGCC
Cuts at: 864 864
Size: 4442
SfoI GGC'GCC
Cuts at: 1874 2122 1874
Size: 248 4194
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
SmaI CCC'GGG
Cuts at: 158 720 1464 158
Size: 562 744 3136
Fragments arranged by size:
3136 744 562
SmlI C'TyrA_G
Cuts at: 1597 2512 3226 3488 1597
Size: 915 714 262 2551
Fragments arranged by size:
2551 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4442
SphI G_CATG'C
Cuts at: 1495 1995 1495
Size: 500 3942
StuI AGG'CCT
Cuts at: 686 686
Size: 4442
StyI C'CwwG_G
Cuts at: 107 446 734 1016 1161 1576 1930 107
Size: 339 288 282 145 415 354 2619
Fragments arranged by size:
2619 415 354 339 288 282 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1130 2025 2158 2617 2826 1130
Size: 895 133 459 209 2746
Fragments arranged by size:
2746 895 459 209 133
TatI w'GTAC_w
Cuts at: 359 3957 359
Size: 3598 844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 442 474 981 1013 3959 3991 442
Size: 32 507 32 2946 32 893
Fragments arranged by size:
2946 893 507 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 697 2239 697
Size: 1542 2900
XbaI T'CTAG_A
Cuts at: 1473 1473
Size: 4442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 256 480 988 1168 1937 256
Size: 224 508 180 769 2761
Fragments arranged by size:
2761 769 508 224 180
XmaI C'CCGG_G
Cuts at: 156 718 1462 156
Size: 562 744 3136
Fragments arranged by size:
3136 744 562
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspXI PsrI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI