Restriction map for
pF1KB8716
(Circular) (Six-base) MAPSORT of: pF1KB8716.seq Check: 4567 from: 1 to: 4742
pF1KB8716 4742 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 487 502 487
Size: 15 4727
AccI GT'mk_AC
Cuts at: 1780 1780
Size: 4742
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2999 3352 4014 2567
Size: 432 353 662 3295
Fragments arranged by size:
3295 662 432 353
AflIII A'CryG_T
Cuts at: 2094 3894 4175 2094
Size: 1800 281 2661
Fragments arranged by size:
2661 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 330 852 2047 3485 330
Size: 522 1195 1438 1587
Fragments arranged by size:
1587 1438 1195 522
ApaI G_GGCC'C
Cuts at: 259 524 1183 1468 1630 259
Size: 265 659 285 162 3371
Fragments arranged by size:
3371 659 285 265 162
ApaLI G'TGCA_C
Cuts at: 441 1255 3580 441
Size: 814 2325 1603
Fragments arranged by size:
2325 1603 814
ApoI r'AATT_y
Cuts at: 1746 4095 4351 1746
Size: 2349 256 2137
Fragments arranged by size:
2349 2137 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 258 903 1176 1408 1442 1458 1607 1762 258
Size: 645 273 232 34 16 149 155 3238
Fragments arranged by size:
3238 645 273 232 155 149 34 16
BamHI G'GATC_C
Cuts at: 1001 1767 1001
Size: 766 3976
BanI G'GyrC_C
Cuts at: 713 952 1273 1299 1383 1758 2172 2420 2455
Size: 239 321 26 84 375 414 248 35
Cuts at: 2455 713
Size: 3000
Fragments arranged by size:
3000 414 375 321 248 239 84 35
26
BanII G_rGCy'C
Cuts at: 167 259 319 524 947 1183 1468 1544 1630
Size: 92 60 205 423 236 285 76 86
Cuts at: 1630 1756 2786 167
Size: 126 1030 2123
Fragments arranged by size:
2123 1030 423 285 236 205 126 92
86 76 60
BbeI G_GCGC'C
Cuts at: 717 956 1387 2176 2424 717
Size: 239 431 789 248 3035
Fragments arranged by size:
3035 789 431 248 239
BbsI GAAGACnn'nnnn_
Cuts at: 1108 1108
Size: 4742
BciVI GTATCCnnnnn_n'
Cuts at: 2640 3696 4742 2640
Size: 1056 1046 2640
Fragments arranged by size:
2640 1056 1046
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglII A'GATC_T
Cuts at: 2257 4294 2257
Size: 2037 2705
BlpI GC'TnA_GC
Cuts at: 415 1854 415
Size: 1439 3303
Bme1580I G_kGCm'C
Cuts at: 119 259 428 445 524 1183 1259 1304 1468
Size: 140 169 17 79 659 76 45 164
Cuts at: 1468 1630 2367 2460 3584 119
Size: 162 737 93 1124 1277
Fragments arranged by size:
1277 1124 737 659 169 164 162 140
93 79 76 45 17
BmgBI CAC'GTC
Cuts at: 1254 1476 1647 1254
Size: 222 171 4349
Fragments arranged by size:
4349 222 171
BmrI ACTGGGnnnn_n'
Cuts at: 1954 2035 2370 4514 1954
Size: 81 335 2144 2182
Fragments arranged by size:
2182 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 367
Size: 4742
Bpu10I CC'TnA_GC
Cuts at: 1555 1555
Size: 4742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1918 2797 3511 3809 1918
Size: 879 714 298 2851
Fragments arranged by size:
2851 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4446 4446
Size: 4742
BsaAI yAC'GTr
Cuts at: 114 399 2095 114
Size: 285 1696 2761
Fragments arranged by size:
2761 1696 285
BsaBI GATnn'nnATC
Cuts at: 6 1687 2256 4293 6
Size: 1681 569 2037 455
Fragments arranged by size:
2037 1681 569 455
BsaHI Gr'CG_yC
Cuts at: 714 881 953 1384 2173 2421 3123 4619 714
Size: 167 72 431 789 248 702 1496 837
Fragments arranged by size:
1496 837 789 702 431 248 167 72
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3541 3688 3962 1931
Size: 521 678 411 147 274 2711
Fragments arranged by size:
2711 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1627 1630 1627
Size: 3 4739
BseYI C'CCAG_C
Cuts at: 123 128 486 925 1368 2167 2925 3590 3998
Size: 5 358 439 443 799 758 665 408
Cuts at: 3998 123
Size: 867
Fragments arranged by size:
867 799 758 665 443 439 408 358
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 931 1567 664
Size: 267 636 3839
Fragments arranged by size:
3839 636 267
BsiEI CG_ry'CG
Cuts at: 104 212 360 982 1718 2284 2330 3560 104
Size: 108 148 622 736 566 46 1230 1286
Fragments arranged by size:
1286 1230 736 622 566 148 108 46
BsiHKAI G_wGCw'C
Cuts at: 167 445 1259 1756 2534 2724 3584 167
Size: 278 814 497 778 190 860 1325
Fragments arranged by size:
1325 860 814 778 497 278 190
Bsp1286I G_dGCh'C
Cuts at: 119 167 259 319 428 445 524 947 1183
Size: 48 92 60 109 17 79 423 236
Cuts at: 1183 1259 1304 1468 1544 1630 1756 2367 2460
Size: 76 45 164 76 86 126 611 93
Cuts at: 2460 2534 2724 2786 3584 119
Size: 74 190 62 798 1277
Fragments arranged by size:
1277 798 611 423 236 190 164 126
109 93 92 86 79 76 76 74
62 60 48 45 17
BspHI T'CATG_A
Cuts at: 605 3174 605
Size: 2569 2173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 487 502 1792 2006 2308 2689 487
Size: 15 1290 214 302 381 2540
Fragments arranged by size:
2540 1290 381 302 214 15
BsrBI CCG'CTC
Cuts at: 50 719 1142 1404 1481 3034 3088 4572 4737
Size: 669 423 262 77 1553 54 1484 165
Cuts at: 4737 50
Size: 55
Fragments arranged by size:
1553 1484 669 423 262 165 77 55
54
BsrDI GCAATG_nn'
Cuts at: 2654 2654
Size: 4742
BsrFI r'CCGG_y
Cuts at: 704 791 2740 2921 704
Size: 87 1949 181 2525
Fragments arranged by size:
2525 1949 181 87
BssHII G'CGCG_C
Cuts at: 221 482 1093 1234 221
Size: 261 611 141 3729
Fragments arranged by size:
3729 611 261 141
BssSI C'ACGA_G
Cuts at: 3013 3721 3013
Size: 708 4034
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstYI r'GATC_y
Cuts at: 1 242 1001 1682 1767 2234 2257 2592 2838
Size: 241 759 681 85 467 23 335 246
Cuts at: 2838 3144 3156 3242 4294 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 759 681 467 449 335 306 246
241 86 85 23 12
BtgI C'CryG_G
Cuts at: 354 524 890 917 968 1109 354
Size: 170 366 27 51 141 3987
Fragments arranged by size:
3987 366 170 141 51 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 558 1644 1674 2692 2873 3036 558
Size: 1086 30 1018 181 163 2264
Fragments arranged by size:
2264 1086 1018 181 163 30
BtsI GCAGTG_nn'
Cuts at: 715 1985 715
Size: 1270 3472
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DraIII CAC_nnn'GTG
Cuts at: 687 687
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 838 2448 3792 838
Size: 1610 1344 1788
Fragments arranged by size:
1788 1610 1344
EaeI y'GGCC_r
Cuts at: 209 357 374 746 979 1046 1106 2327 2501
Size: 148 17 372 233 67 60 1221 174
Cuts at: 2501 2892 2919 4264 209
Size: 391 27 1345 687
Fragments arranged by size:
1345 1221 687 391 372 233 174 148
67 60 27 17
EagI C'GGCC_G
Cuts at: 209 357 979 2327 209
Size: 148 622 1348 2624
Fragments arranged by size:
2624 1348 622 148
EarI CTCTTCn'nnn_
Cuts at: 307 646 2765 2975 307
Size: 339 2119 210 2074
Fragments arranged by size:
2119 2074 339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1090 1134 1178 1369 1552 2950 3692 3838 4552
Size: 44 44 191 183 1398 742 146 714
Cuts at: 4552 1090
Size: 1280
Fragments arranged by size:
1398 1280 742 714 191 183 146 44
44
EcoICRI GAG'CTC
Cuts at: 165 1754 165
Size: 1589 3153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 2567 2999 3352 4014 367
Size: 2200 432 353 662 1095
Fragments arranged by size:
2200 1095 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 178 1348 1531 1639 178
Size: 1170 183 108 3281
Fragments arranged by size:
3281 1170 183 108
EcoO109I rG'GnC_Cy
Cuts at: 255 520 1180 1464 1626 1627 1653 1881 255
Size: 265 660 284 162 1 26 228 3116
Fragments arranged by size:
3116 660 284 265 228 162 26 1
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 678 2761 678
Size: 2083 2659
FspI TGC'GCA
Cuts at: 2523 2523
Size: 4742
HaeII r_GCGC'y
Cuts at: 354 422 717 862 932 956 1387 2176 2424
Size: 68 295 145 70 24 431 789 248
Cuts at: 2424 3654 4425 354
Size: 1230 771 671
Fragments arranged by size:
1230 789 771 671 431 295 248 145
70 68 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2584 2614 2616 2646 2584
Size: 30 2 30 4680
Fragments arranged by size:
4680 30 30 2
HincII GTy'rAC
Cuts at: 627 1781 627
Size: 1154 3588
KasI G'GCGC_C
Cuts at: 713 952 1383 2172 2420 713
Size: 239 431 789 248 3035
Fragments arranged by size:
3035 789 431 248 239
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 896 3501 3685 896
Size: 2605 184 1953
Fragments arranged by size:
2605 1953 184
MscI TGG'CCA
Cuts at: 376 748 1048 2503 376
Size: 372 300 1455 2615
Fragments arranged by size:
2615 1455 372 300
MslI CAynn'nnrTG
Cuts at: 112 1057 2731 2858 112
Size: 945 1674 127 1996
Fragments arranged by size:
1996 1674 945 127
MspA1I CmG'CkG
Cuts at: 356 526 849 1044 1131 1578 1853 2167 2527
Size: 170 323 195 87 447 275 314 360
Cuts at: 2527 3311 3556 356
Size: 784 245 1542
Fragments arranged by size:
1542 784 447 360 323 314 275 245
195 170 87
NaeI GCC'GGC
Cuts at: 706 2923 706
Size: 2217 2525
NarI GG'CG_CC
Cuts at: 714 953 1384 2173 2421 714
Size: 239 431 789 248 3035
Fragments arranged by size:
3035 789 431 248 239
NgoMIV G'CCGG_C
Cuts at: 704 2921 704
Size: 2217 2525
NotI GC'GGCC_GC
Cuts at: 357 979 357
Size: 622 4120
NspI r_CATG'y
Cuts at: 1795 2295 3898 1795
Size: 500 1603 2639
Fragments arranged by size:
2639 1603 500
PciI A'CATG_T
Cuts at: 3894 3894
Size: 4742
PfoI T'CCnGG_A
Cuts at: 1321 1321
Size: 4742
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PmlI CAC'GTG
Cuts at: 399 399
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 1653 1653
Size: 4742
PspOMI G'GGCC_C
Cuts at: 255 520 1179 1464 1626 255
Size: 265 659 285 162 3371
Fragments arranged by size:
3371 659 285 265 162
PstI C_TGCA'G
Cuts at: 182 566 1789 2474 182
Size: 384 1223 685 2450
Fragments arranged by size:
2450 1223 685 384
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 849 2167 2527 849
Size: 1318 360 3064
Fragments arranged by size:
3064 1318 360
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 167 1756 167
Size: 1589 3153
SacII CC_GC'GG
Cuts at: 357 527 357
Size: 170 4572
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 307 2765 2975 307
Size: 2458 210 2074
Fragments arranged by size:
2458 2074 210
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 1725 4259 1725
Size: 2534 2208
SfcI C'TryA_G
Cuts at: 33 178 562 1785 2470 3438 3629 33
Size: 145 384 1223 685 968 191 1146
Fragments arranged by size:
1223 1146 968 685 384 191 145
SfoI GGC'GCC
Cuts at: 715 954 1385 2174 2422 715
Size: 239 431 789 248 3035
Fragments arranged by size:
3035 789 431 248 239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 260 1178 1444 1609 1764 260
Size: 918 266 165 155 3238
Fragments arranged by size:
3238 918 266 165 155
SmlI C'TyrA_G
Cuts at: 1897 2812 3526 3788 1897
Size: 915 714 262 2851
Fragments arranged by size:
2851 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1795 2295 1795
Size: 500 4242
SrfI GCCC'GGGC
Cuts at: 260 1444 260
Size: 1184 3558
StyI C'CwwG_G
Cuts at: 251 1286 1876 2230 251
Size: 1035 590 354 2763
Fragments arranged by size:
2763 1035 590 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2325 2458 2917 3126 2325
Size: 133 459 209 3941
Fragments arranged by size:
3941 459 209 133
TatI w'GTAC_w
Cuts at: 1723 4257 1723
Size: 2534 2208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1667 1699 4259 4291 1667
Size: 32 2560 32 2118
Fragments arranged by size:
2560 2118 32 32
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 384 1056 2237 384
Size: 672 1181 2889
Fragments arranged by size:
2889 1181 672
XmaI C'CCGG_G
Cuts at: 258 1176 1442 1607 1762 258
Size: 918 266 165 155 3238
Fragments arranged by size:
3238 918 266 165 155
XmnI GAAnn'nnTTC
Cuts at: 331 472 331
Size: 141 4601
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BsaXI BsiWI BsmI BsmBI BspEI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NruI NsiI PacI
PasI PflMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SspI StuI SwaI XhoI ZraI