Restriction map for
pF1KB8719
(Circular) (Six-base) MAPSORT of: pF1KB8719.seq Check: 53 from: 1 to: 4541
pF1KB8719 4541 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 825 881 825
Size: 56 4485
AccI GT'mk_AC
Cuts at: 1579 1579
Size: 4541
Acc65I G'GTAC_C
Cuts at: 1557 1557
Size: 4541
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 1198 2366 2798 3151 3813 858
Size: 340 1168 432 353 662 1586
Fragments arranged by size:
1586 1168 662 432 353 340
AflIII A'CryG_T
Cuts at: 1893 3693 3974 1893
Size: 1800 281 2460
Fragments arranged by size:
2460 1800 281
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 4541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1523 1523
Size: 4541
AlwNI CAG_nnn'CTG
Cuts at: 457 1846 3284 457
Size: 1389 1438 1714
Fragments arranged by size:
1714 1438 1389
ApaI G_GGCC'C
Cuts at: 952 1347 952
Size: 395 4146
ApaLI G'TGCA_C
Cuts at: 3379 3379
Size: 4541
ApoI r'AATT_y
Cuts at: 771 1545 3894 4150 771
Size: 774 2349 256 1162
Fragments arranged by size:
2349 1162 774 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 165 392 986 1561 165
Size: 227 594 575 3145
Fragments arranged by size:
3145 594 575 227
BamHI G'GATC_C
Cuts at: 1566 1566
Size: 4541
BanI G'GyrC_C
Cuts at: 893 1434 1557 1971 2219 2254 893
Size: 541 123 414 248 35 3180
Fragments arranged by size:
3180 541 414 248 123 35
BanII G_rGCy'C
Cuts at: 401 952 1282 1347 1555 2585 401
Size: 551 330 65 208 1030 2357
Fragments arranged by size:
2357 1030 551 330 208 65
BbeI G_GCGC'C
Cuts at: 1438 1975 2223 1438
Size: 537 248 3756
Fragments arranged by size:
3756 537 248
BbsI GAAGACnn'nnnn_
Cuts at: 549 549
Size: 4541
BbvCI CC'TCA_GC
Cuts at: 659 659
Size: 4541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 113 147 1180 1214 113
Size: 34 1033 34 3440
Fragments arranged by size:
3440 1033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2439 3495 4541 2439
Size: 1056 1046 2439
Fragments arranged by size:
2439 1056 1046
BclI T'GATC_A
Cuts at: 2061 2061
Size: 4541
BglI GCCn_nnn'nGGC
Cuts at: 956 1192 1351 956
Size: 236 159 4146
Fragments arranged by size:
4146 236 159
BglII A'GATC_T
Cuts at: 2056 4093 2056
Size: 2037 2504
BlpI GC'TnA_GC
Cuts at: 1653 1653
Size: 4541
Bme1580I G_kGCm'C
Cuts at: 636 952 1347 2166 2259 3383 636
Size: 316 395 819 93 1124 1794
Fragments arranged by size:
1794 1124 819 395 316 93
BmrI ACTGGGnnnn_n'
Cuts at: 1753 1834 2169 4313 1753
Size: 81 335 2144 1981
Fragments arranged by size:
2144 1981 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 277 404 479 520 536 886 1168 232
Size: 45 127 75 41 16 350 282 3605
Fragments arranged by size:
3605 350 282 127 75 45 41 16
Bpu10I CC'TnA_GC
Cuts at: 562 659 886 1276 562
Size: 97 227 390 3827
Fragments arranged by size:
3827 390 227 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1104 1375 1717 2596 3310 3608 1104
Size: 271 342 879 714 298 2037
Fragments arranged by size:
2037 879 714 342 298 271
BsaI GGTCTCn'nnnn_
Cuts at: 742 4245 742
Size: 3503 1038
BsaAI yAC'GTr
Cuts at: 1113 1894 1113
Size: 781 3760
BsaBI GATnn'nnATC
Cuts at: 6 358 2055 4092 6
Size: 352 1697 2037 455
Fragments arranged by size:
2037 1697 455 352
BsaHI Gr'CG_yC
Cuts at: 115 1435 1972 2220 2922 4418 115
Size: 1320 537 248 702 1496 238
Fragments arranged by size:
1496 1320 702 537 248 238
BsaWI w'CCGG_w
Cuts at: 1730 2251 2929 3340 3487 3761 1730
Size: 521 678 411 147 274 2510
Fragments arranged by size:
2510 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 574 604 802 832 1462 1492 574
Size: 30 198 30 630 30 3623
Fragments arranged by size:
3623 630 198 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 535 538 541 823 337
Size: 198 3 3 282 4055
Fragments arranged by size:
4055 282 198 3 3
BseYI C'CCAG_C
Cuts at: 190 544 926 1131 1170 1281 1371 1966 2724
Size: 354 382 205 39 111 90 595 758
Cuts at: 2724 3389 3797 190
Size: 665 408 934
Fragments arranged by size:
934 758 665 595 408 382 354 205
111 90 39
BsiEI CG_ry'CG
Cuts at: 104 492 2083 2129 3359 104
Size: 388 1591 46 1230 1286
Fragments arranged by size:
1591 1286 1230 388 46
BsiHKAI G_wGCw'C
Cuts at: 716 1555 2333 2523 3383 716
Size: 839 778 190 860 1874
Fragments arranged by size:
1874 860 839 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 4541
Bsp1286I G_dGCh'C
Cuts at: 401 636 716 952 1282 1347 1555 2166 2259
Size: 235 80 236 330 65 208 611 93
Cuts at: 2259 2333 2523 2585 3383 401
Size: 74 190 62 798 1559
Fragments arranged by size:
1559 798 611 330 236 235 208 190
93 80 74 65 62
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 825 881 1502 1591 1805 2107 2488 825
Size: 56 621 89 214 302 381 2878
Fragments arranged by size:
2878 621 381 302 214 89 56
BsrBI CCG'CTC
Cuts at: 50 1167 2833 2887 4371 4536 50
Size: 1117 1666 54 1484 165 55
Fragments arranged by size:
1666 1484 1117 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2453 2453
Size: 4541
BsrFI r'CCGG_y
Cuts at: 1431 2539 2720 1431
Size: 1108 181 3252
Fragments arranged by size:
3252 1108 181
BssSI C'ACGA_G
Cuts at: 573 2812 3520 573
Size: 2239 708 1594
Fragments arranged by size:
2239 1594 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1918 1918
Size: 4541
BstBI TT'CG_AA
Cuts at: 2902 2902
Size: 4541
BstYI r'GATC_y
Cuts at: 1 1142 1566 2033 2056 2391 2637 2943 2955
Size: 1141 424 467 23 335 246 306 12
Cuts at: 2955 3041 4093 1
Size: 86 1052 449
Fragments arranged by size:
1141 1052 467 449 424 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 440 862 440
Size: 422 4119
BtgI C'CryG_G
Cuts at: 107 486 107
Size: 379 4162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2491 2672 2835 2491
Size: 181 163 4197
Fragments arranged by size:
4197 181 163
BtsI GCAGTG_nn'
Cuts at: 1784 1784
Size: 4541
DraI TTT'AAA
Cuts at: 1540 1540
Size: 4541
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3591 2247
Size: 1344 3197
EaeI y'GGCC_r
Cuts at: 489 614 1184 1253 1322 1400 2126 2300 2691
Size: 125 570 69 69 78 726 174 391
Cuts at: 2691 2718 4063 489
Size: 27 1345 967
Fragments arranged by size:
1345 967 726 570 391 174 125 78
69 69 27
EagI C'GGCC_G
Cuts at: 489 2126 489
Size: 1637 2904
EarI CTCTTCn'nnn_
Cuts at: 224 659 670 2564 2774 224
Size: 435 11 1894 210 1991
Fragments arranged by size:
1991 1894 435 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2749 3491 3637 4351 2749
Size: 742 146 714 2939
Fragments arranged by size:
2939 742 714 146
EcoICRI GAG'CTC
Cuts at: 1553 1553
Size: 4541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 277 404 479 520 536 858 886 1168
Size: 45 127 75 41 16 322 28 282
Cuts at: 1168 1198 2366 2798 3151 3813 232
Size: 30 1168 432 353 662 960
Fragments arranged by size:
1168 960 662 432 353 322 282 127
75 45 41 30 28 16
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 4541
EcoO109I rG'GnC_Cy
Cuts at: 161 216 243 482 867 949 1344 1470 1680
Size: 55 27 239 385 82 395 126 210
Cuts at: 1680 161
Size: 3022
Fragments arranged by size:
3022 395 385 239 210 126 82 55
27
EcoRI G'AATT_C
Cuts at: 771 1545 771
Size: 774 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4541
FspI TGC'GCA
Cuts at: 2322 2322
Size: 4541
HaeII r_GCGC'y
Cuts at: 1308 1438 1975 2223 3453 4224 1308
Size: 130 537 248 1230 771 1625
Fragments arranged by size:
1625 1230 771 537 248 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1233 1265 1462 1494 2383 2413 2415
Size: 32 627 32 197 32 889 30 2
Cuts at: 2415 2445 574
Size: 30 2670
Fragments arranged by size:
2670 889 627 197 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1580 1580
Size: 4541
HindIII A'AGCT_T
Cuts at: 1520 1520
Size: 4541
KasI G'GCGC_C
Cuts at: 1434 1971 2219 1434
Size: 537 248 3756
Fragments arranged by size:
3756 537 248
KpnI G_GTAC'C
Cuts at: 1561 1561
Size: 4541
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1079 3300 3484 1079
Size: 2221 184 2136
Fragments arranged by size:
2221 2136 184
MscI TGG'CCA
Cuts at: 616 1186 1255 2302 616
Size: 570 69 1047 2855
Fragments arranged by size:
2855 1047 570 69
MslI CAynn'nnrTG
Cuts at: 460 610 2530 2657 460
Size: 150 1920 127 2344
Fragments arranged by size:
2344 1920 150 127
MspA1I CmG'CkG
Cuts at: 213 488 540 816 1198 1652 1966 2326 3110
Size: 275 52 276 382 454 314 360 784
Cuts at: 3110 3355 213
Size: 245 1399
Fragments arranged by size:
1399 784 454 382 360 314 276 275
245 52
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 4541
NarI GG'CG_CC
Cuts at: 1435 1972 2220 1435
Size: 537 248 3756
Fragments arranged by size:
3756 537 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4541
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 4541
NspI r_CATG'y
Cuts at: 451 1594 2094 3697 451
Size: 1143 500 1603 1295
Fragments arranged by size:
1603 1295 1143 500
PciI A'CATG_T
Cuts at: 3693 3693
Size: 4541
PfoI T'CCnGG_A
Cuts at: 218 596 1145 218
Size: 378 549 3614
Fragments arranged by size:
3614 549 378
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 4509
PpuMI rG'GwC_Cy
Cuts at: 216 867 1470 216
Size: 651 603 3287
Fragments arranged by size:
3287 651 603
PspOMI G'GGCC_C
Cuts at: 948 1343 948
Size: 395 4146
PspXI vC'TCGA_Gb
Cuts at: 986 986
Size: 4541
PstI C_TGCA'G
Cuts at: 1415 1502 1588 2273 1415
Size: 87 86 685 3683
Fragments arranged by size:
3683 685 87 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 816 1966 2326 816
Size: 1150 360 3031
Fragments arranged by size:
3031 1150 360
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 1555 1555
Size: 4541
SacII CC_GC'GG
Cuts at: 489 489
Size: 4541
SalI G'TCGA_C
Cuts at: 1578 1578
Size: 4541
SanDI GG'GwC_CC
Cuts at: 867 867
Size: 4541
SapI GCTCTTCn'nnn_
Cuts at: 2564 2774 2564
Size: 210 4331
SbfI CC_TGCA'GG
Cuts at: 1588 1588
Size: 4541
ScaI AGT'ACT
Cuts at: 4058 4058
Size: 4541
SfcI C'TryA_G
Cuts at: 33 1300 1411 1498 1584 2269 3237 3428 33
Size: 1267 111 87 86 685 968 191 1146
Fragments arranged by size:
1267 1146 968 685 191 111 87 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 1192 1351 1192
Size: 159 4382
SfoI GGC'GCC
Cuts at: 1436 1973 2221 1436
Size: 537 248 3756
Fragments arranged by size:
3756 537 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SgrAI Cr'CCGG_yG
Cuts at: 1431 1431
Size: 4541
SmaI CCC'GGG
Cuts at: 394 1563 394
Size: 1169 3372
SmlI C'TyrA_G
Cuts at: 986 1083 1390 1696 2611 3325 3587 986
Size: 97 307 306 915 714 262 1940
Fragments arranged by size:
1940 915 714 307 306 262 97
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 451 1594 2094 451
Size: 1143 500 2898
Fragments arranged by size:
2898 1143 500
StuI AGG'CCT
Cuts at: 497 1274 497
Size: 777 3764
StyI C'CwwG_G
Cuts at: 107 377 617 1675 2029 107
Size: 270 240 1058 354 2619
Fragments arranged by size:
2619 1058 354 270 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 982 1399 2124 2257 2716 2925 982
Size: 417 725 133 459 209 2598
Fragments arranged by size:
2598 725 459 417 209 133
TatI w'GTAC_w
Cuts at: 758 782 1331 4056 758
Size: 24 549 2725 1243
Fragments arranged by size:
2725 1243 549 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 4509
Tth111I GACn'n_nGTC
Cuts at: 2338 2338
Size: 4541
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2036 2036
Size: 4541
XhoI C'TCGA_G
Cuts at: 986 986
Size: 4541
XmaI C'CCGG_G
Cuts at: 392 1561 392
Size: 1169 3372
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsgI BsiWI BsmI BspEI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PsrI SexAI SnaBI SrfI SspI SwaI XmnI ZraI