Restriction map for
pF1KB8721
(Circular) (Six-base) MAPSORT of: pF1KB8721.seq Check: 7172 from: 1 to: 4784
pF1KB8721 4784 bp
With 182 enzymes: SgfI *
July 24, 2008 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 212 1227 212
Size: 1015 3769
AccI GT'mk_AC
Cuts at: 1004 1283 1822 1004
Size: 279 539 3966
Fragments arranged by size:
3966 539 279
Acc65I G'GTAC_C
Cuts at: 1800 1800
Size: 4784
AclI AA'CG_TT
Cuts at: 1778 4624 1778
Size: 2846 1938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 496 1462 1574 2609 3041 3394 4056 110
Size: 386 966 112 1035 432 353 662 838
Fragments arranged by size:
1035 966 838 662 432 386 353 112
AflIII A'CryG_T
Cuts at: 2136 3936 4217 2136
Size: 1800 281 2703
Fragments arranged by size:
2703 1800 281
AleI CACnn'nnGTG
Cuts at: 1523 1523
Size: 4784
AlwNI CAG_nnn'CTG
Cuts at: 150 234 474 1170 1260 1342 1630 2089 3527
Size: 84 240 696 90 82 288 459 1438
Cuts at: 3527 150
Size: 1407
Fragments arranged by size:
1438 1407 696 459 288 240 90 84
82
ApaI G_GGCC'C
Cuts at: 345 600 1406 1440 345
Size: 255 806 34 3689
Fragments arranged by size:
3689 806 255 34
ApaLI G'TGCA_C
Cuts at: 312 1516 3622 312
Size: 1204 2106 1474
Fragments arranged by size:
2106 1474 1204
ApoI r'AATT_y
Cuts at: 1788 4137 4393 1788
Size: 2349 256 2179
Fragments arranged by size:
2349 2179 256
AseI AT'TA_AT
Cuts at: 20 977 20
Size: 957 3827
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
AvaI C'yCGr_G
Cuts at: 174 733 765 1804 174
Size: 559 32 1039 3154
Fragments arranged by size:
3154 1039 559 32
BamHI G'GATC_C
Cuts at: 1809 1809
Size: 4784
BanI G'GyrC_C
Cuts at: 820 1800 2214 2462 2497 820
Size: 980 414 248 35 3107
Fragments arranged by size:
3107 980 414 248 35
BanII G_rGCy'C
Cuts at: 175 200 345 600 1406 1440 1798 2828 175
Size: 25 145 255 806 34 358 1030 2131
Fragments arranged by size:
2131 1030 806 358 255 145 34 25
BbeI G_GCGC'C
Cuts at: 2218 2466 2218
Size: 248 4536
BbsI GAAGACnn'nnnn_
Cuts at: 1729 1729
Size: 4784
BbvCI CC'TCA_GC
Cuts at: 1631 1631
Size: 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 408 442 408
Size: 34 4750
BciVI GTATCCnnnnn_n'
Cuts at: 2682 3738 4784 2682
Size: 1056 1046 2682
Fragments arranged by size:
2682 1056 1046
BclI T'GATC_A
Cuts at: 2304 2304
Size: 4784
BglI GCCn_nnn'nGGC
Cuts at: 147 269 673 829 1410 147
Size: 122 404 156 581 3521
Fragments arranged by size:
3521 581 404 156 122
BglII A'GATC_T
Cuts at: 1700 2299 4336 1700
Size: 599 2037 2148
Fragments arranged by size:
2148 2037 599
BlpI GC'TnA_GC
Cuts at: 208 1479 1896 208
Size: 1271 417 3096
Fragments arranged by size:
3096 1271 417
Bme1580I G_kGCm'C
Cuts at: 316 345 600 1406 1440 1520 2409 2502 3626
Size: 29 255 806 34 80 889 93 1124
Cuts at: 3626 316
Size: 1474
Fragments arranged by size:
1474 1124 889 806 255 93 80 34
29
BmgBI CAC'GTC
Cuts at: 1494 1494
Size: 4784
BmrI ACTGGGnnnn_n'
Cuts at: 1400 1996 2077 2412 4556 1400
Size: 596 81 335 2144 1628
Fragments arranged by size:
2144 1628 596 335 81
BmtI G_CTAG'C
Cuts at: 964 964
Size: 4784
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3151 3151
Size: 4784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 367 373 766 154
Size: 213 6 393 4172
Fragments arranged by size:
4172 393 213 6
Bpu10I CC'TnA_GC
Cuts at: 707 1631 707
Size: 924 3860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1960 2839 3553 3851 1960
Size: 879 714 298 2893
Fragments arranged by size:
2893 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1006 1067 4488 1006
Size: 61 3421 1302
Fragments arranged by size:
3421 1302 61
BsaAI yAC'GTr
Cuts at: 2137 2137
Size: 4784
BsaBI GATnn'nnATC
Cuts at: 6 1072 2298 4335 6
Size: 1066 1226 2037 455
Fragments arranged by size:
2037 1226 1066 455
BsaHI Gr'CG_yC
Cuts at: 2215 2463 3165 4661 2215
Size: 248 702 1496 2338
Fragments arranged by size:
2338 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1973 2494 3172 3583 3730 4004 1973
Size: 521 678 411 147 274 2753
Fragments arranged by size:
2753 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 293 323 293
Size: 30 4754
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 205 208 364 751 1024 192
Size: 13 3 156 387 273 3952
Fragments arranged by size:
3952 387 273 156 13 3
BseYI C'CCAG_C
Cuts at: 143 360 671 1170 1342 2209 2967 3632 4040
Size: 217 311 499 172 867 758 665 408
Cuts at: 4040 143
Size: 887
Fragments arranged by size:
887 867 758 665 499 408 311 217
172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 1289
Size: 4784
BsiEI CG_ry'CG
Cuts at: 104 273 2326 2372 3602 104
Size: 169 2053 46 1230 1286
Fragments arranged by size:
2053 1286 1230 169 46
BsiHKAI G_wGCw'C
Cuts at: 316 359 1520 1798 2576 2766 3626 316
Size: 43 1161 278 778 190 860 1474
Fragments arranged by size:
1474 1161 860 778 278 190 43
BsmI GAATG_Cn'
Cuts at: 1417 1476 1417
Size: 59 4725
Bsp1286I G_dGCh'C
Cuts at: 175 200 316 345 359 600 1406 1440 1520
Size: 25 116 29 14 241 806 34 80
Cuts at: 1520 1798 2409 2502 2576 2766 2828 3626 175
Size: 278 611 93 74 190 62 798 1333
Fragments arranged by size:
1333 806 798 611 278 241 190 116
93 80 74 62 34 29 25 14
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 212 1227 1834 2048 2350 2731 212
Size: 1015 607 214 302 381 2265
Fragments arranged by size:
2265 1015 607 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3076 3130 4614 4779 50
Size: 3026 54 1484 165 55
Fragments arranged by size:
3026 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2696 2696
Size: 4784
BsrFI r'CCGG_y
Cuts at: 272 481 2782 2963 272
Size: 209 2301 181 2093
Fragments arranged by size:
2301 2093 209 181
BsrGI T'GTAC_A
Cuts at: 1207 1207
Size: 4784
BssHII G'CGCG_C
Cuts at: 725 725
Size: 4784
BssSI C'ACGA_G
Cuts at: 1648 3055 3763 1648
Size: 1407 708 2669
Fragments arranged by size:
2669 1407 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1471 2161 1471
Size: 690 4094
BstBI TT'CG_AA
Cuts at: 3145 3145
Size: 4784
BstEII G'GTnAC_C
Cuts at: 288 288
Size: 4784
BstXI CCAn_nnnn'nTGG
Cuts at: 368 657 1356 368
Size: 289 699 3796
Fragments arranged by size:
3796 699 289
BstYI r'GATC_y
Cuts at: 1 585 1320 1700 1809 2276 2299 2634 2880
Size: 584 735 380 109 467 23 335 246
Cuts at: 2880 3186 3198 3284 4336 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 735 584 467 449 380 335 306
246 109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 337 337
Size: 4784
BtgI C'CryG_G
Cuts at: 107 267 518 635 1287 107
Size: 160 251 117 652 3604
Fragments arranged by size:
3604 652 251 160 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2734 2915 3078 2734
Size: 181 163 4440
Fragments arranged by size:
4440 181 163
BtsI GCAGTG_nn'
Cuts at: 326 524 544 992 1631 2027 326
Size: 198 20 448 639 396 3083
Fragments arranged by size:
3083 639 448 396 198 20
DraI TTT'AAA
Cuts at: 1783 1783
Size: 4784
DraIII CAC_nnn'GTG
Cuts at: 320 320
Size: 4784
DrdI GACnn_nn'nnGTC
Cuts at: 118 2490 3834 118
Size: 2372 1344 1068
Fragments arranged by size:
2372 1344 1068
EaeI y'GGCC_r
Cuts at: 236 270 521 539 557 803 1673 2369 2543
Size: 34 251 18 18 246 870 696 174
Cuts at: 2543 2934 2961 4306 236
Size: 391 27 1345 714
Fragments arranged by size:
1345 870 714 696 391 251 246 174
34 27 18 18
EagI C'GGCC_G
Cuts at: 270 2369 270
Size: 2099 2685
EarI CTCTTCn'nnn_
Cuts at: 182 2807 3017 182
Size: 2625 210 1949
Fragments arranged by size:
2625 1949 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1042 2992 3734 3880 4594 1042
Size: 1950 742 146 714 1232
Fragments arranged by size:
1950 1232 742 714 146
EcoICRI GAG'CTC
Cuts at: 1796 1796
Size: 4784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 154 367 373 496 766 1462 1574 2609
Size: 44 213 6 123 270 696 112 1035
Cuts at: 2609 3041 3394 4056 110
Size: 432 353 662 838
Fragments arranged by size:
1035 838 696 662 432 353 270 213
123 112 44 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1087 1087
Size: 4784
EcoO109I rG'GnC_Cy
Cuts at: 341 342 534 574 597 1402 1437 1923 341
Size: 1 192 40 23 805 35 486 3202
Fragments arranged by size:
3202 805 486 192 40 35 23 1
EcoRI G'AATT_C
Cuts at: 1788 1788
Size: 4784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4784
FseI GG_CCGG'CC
Cuts at: 485 485
Size: 4784
FspI TGC'GCA
Cuts at: 2565 2565
Size: 4784
HaeII r_GCGC'y
Cuts at: 2218 2466 3696 4467 2218
Size: 248 1230 771 2535
Fragments arranged by size:
2535 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1692 1724 2626 2656 2658 2688 1692
Size: 32 902 30 2 30 3788
Fragments arranged by size:
3788 902 32 30 30 2
HincII GTy'rAC
Cuts at: 1005 1823 1005
Size: 818 3966
KasI G'GCGC_C
Cuts at: 2214 2462 2214
Size: 248 4536
KpnI G_GTAC'C
Cuts at: 1804 1804
Size: 4784
MluI A'CGCG_T
Cuts at: 4217 4217
Size: 4784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1076 3543 3727 1076
Size: 2467 184 2133
Fragments arranged by size:
2467 2133 184
MscI TGG'CCA
Cuts at: 238 541 559 805 1675 2545 238
Size: 303 18 246 870 870 2477
Fragments arranged by size:
2477 870 870 303 246 18
MslI CAynn'nnrTG
Cuts at: 866 1045 1354 1523 2773 2900 866
Size: 179 309 169 1250 127 2750
Fragments arranged by size:
2750 1250 309 179 169 127
MspA1I CmG'CkG
Cuts at: 147 471 967 1895 2209 2569 3353 3598 147
Size: 324 496 928 314 360 784 245 1333
Fragments arranged by size:
1333 928 784 496 360 324 314 245
NaeI GCC'GGC
Cuts at: 274 483 2965 274
Size: 209 2482 2093
Fragments arranged by size:
2482 2093 209
NarI GG'CG_CC
Cuts at: 2215 2463 2215
Size: 248 4536
NcoI C'CATG_G
Cuts at: 107 635 107
Size: 528 4256
NgoMIV G'CCGG_C
Cuts at: 272 481 2963 272
Size: 209 2482 2093
Fragments arranged by size:
2482 2093 209
NheI G'CTAG_C
Cuts at: 960 960
Size: 4784
NspI r_CATG'y
Cuts at: 1837 2337 3940 1837
Size: 500 1603 2681
Fragments arranged by size:
2681 1603 500
PasI CC'CwG_GG
Cuts at: 1254 1254
Size: 4784
PciI A'CATG_T
Cuts at: 3936 3936
Size: 4784
PflMI CCAn_nnn'nTGG
Cuts at: 150 234 570 1342 150
Size: 84 336 772 3592
Fragments arranged by size:
3592 772 336 84
PfoI T'CCnGG_A
Cuts at: 377 503 1335 377
Size: 126 832 3826
Fragments arranged by size:
3826 832 126
PmeI GTTT'AAAC
Cuts at: 1783 1783
Size: 4784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4752
PpuMI rG'GwC_Cy
Cuts at: 534 534
Size: 4784
PspOMI G'GGCC_C
Cuts at: 341 596 1402 1436 341
Size: 255 806 34 3689
Fragments arranged by size:
3689 806 255 34
PstI C_TGCA'G
Cuts at: 225 531 972 1831 2516 225
Size: 306 441 859 685 2493
Fragments arranged by size:
2493 859 685 441 306
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4784
PvuII CAG'CTG
Cuts at: 471 967 2209 2569 471
Size: 496 1242 360 2686
Fragments arranged by size:
2686 1242 496 360
RsrII CG'GwC_CG
Cuts at: 2979 2979
Size: 4784
SacI G_AGCT'C
Cuts at: 1798 1798
Size: 4784
SalI G'TCGA_C
Cuts at: 1003 1821 1003
Size: 818 3966
SapI GCTCTTCn'nnn_
Cuts at: 2807 3017 2807
Size: 210 4574
SbfI CC_TGCA'GG
Cuts at: 1831 1831
Size: 4784
ScaI AGT'ACT
Cuts at: 4301 4301
Size: 4784
SfcI C'TryA_G
Cuts at: 33 221 527 968 1642 1655 1827 2512 3480
Size: 188 306 441 674 13 172 685 968
Cuts at: 3480 3671 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 674 441 306 191 188
172 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 269 1410 269
Size: 1141 3643
SfoI GGC'GCC
Cuts at: 2216 2464 2216
Size: 248 4536
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
SmaI CCC'GGG
Cuts at: 735 767 1806 735
Size: 32 1039 3713
Fragments arranged by size:
3713 1039 32
SmlI C'TyrA_G
Cuts at: 1939 2854 3568 3830 1939
Size: 915 714 262 2893
Fragments arranged by size:
2893 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4784
SphI G_CATG'C
Cuts at: 1837 2337 1837
Size: 500 4284
StuI AGG'CCT
Cuts at: 511 511
Size: 4784
StyI C'CwwG_G
Cuts at: 107 163 635 1521 1611 1764 1918 2272 107
Size: 56 472 886 90 153 154 354 2619
Fragments arranged by size:
2619 886 472 354 154 153 90 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 374 1267 1531 1751 2367 2500 2959 3168 374
Size: 893 264 220 616 133 459 209 1990
Fragments arranged by size:
1990 893 616 459 264 220 209 133
TatI w'GTAC_w
Cuts at: 1207 4299 1207
Size: 3092 1692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 293 325 613 645 777 809 965 997 1570
Size: 32 288 32 132 32 156 32 573
Cuts at: 1570 1602 4301 4333 293
Size: 32 2699 32 744
Fragments arranged by size:
2699 744 573 288 156 132 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2581 2581
Size: 4784
XbaI T'CTAG_A
Cuts at: 1815 1815
Size: 4784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 567 2279 567
Size: 1712 3072
XmaI C'CCGG_G
Cuts at: 733 765 1804 733
Size: 32 1039 3713
Fragments arranged by size:
3713 1039 32
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BfrBI BsiWI BsmBI BspEI BstZ17I ClaI
CspCI EcoRV FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI