Restriction map for
pF1KB8724
(Circular) (Six-base) MAPSORT of: pF1KB8724.seq Check: 8628 from: 1 to: 4694
pF1KB8724 4694 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 479 1604 1732 479
Size: 1125 128 3441
Fragments arranged by size:
3441 1125 128
Acc65I G'GTAC_C
Cuts at: 1710 1710
Size: 4694
AclI AA'CG_TT
Cuts at: 4534 4534
Size: 4694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 804 1438 2519 2951 3304 3966 718
Size: 86 634 1081 432 353 662 1446
Fragments arranged by size:
1446 1081 662 634 432 353 86
AflIII A'CryG_T
Cuts at: 2046 3846 4127 2046
Size: 1800 281 2613
Fragments arranged by size:
2613 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 486 486
Size: 4694
AleI CACnn'nnGTG
Cuts at: 1552 1552
Size: 4694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1270 1270
Size: 4694
AlwNI CAG_nnn'CTG
Cuts at: 508 958 1209 1999 3437 508
Size: 450 251 790 1438 1765
Fragments arranged by size:
1765 1438 790 450 251
ApaLI G'TGCA_C
Cuts at: 721 3532 721
Size: 2811 1883
ApoI r'AATT_y
Cuts at: 828 1096 1378 1698 4047 4303 828
Size: 268 282 320 2349 256 1219
Fragments arranged by size:
2349 1219 320 282 268 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 1316 1358 1714 1316
Size: 42 356 4296
Fragments arranged by size:
4296 356 42
BamHI G'GATC_C
Cuts at: 557 1081 1719 557
Size: 524 638 3532
Fragments arranged by size:
3532 638 524
BanI G'GyrC_C
Cuts at: 1537 1710 2124 2372 2407 1537
Size: 173 414 248 35 3824
Fragments arranged by size:
3824 414 248 173 35
BanII G_rGCy'C
Cuts at: 1708 2738 1708
Size: 1030 3664
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BbsI GAAGACnn'nnnn_
Cuts at: 461 1297 461
Size: 836 3858
BbvCI CC'TCA_GC
Cuts at: 929 929
Size: 4694
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3648 4694 2592
Size: 1056 1046 2592
Fragments arranged by size:
2592 1056 1046
BclI T'GATC_A
Cuts at: 2214 2214
Size: 4694
BglII A'GATC_T
Cuts at: 2209 4246 2209
Size: 2037 2657
BlpI GC'TnA_GC
Cuts at: 1806 1806
Size: 4694
Bme1580I G_kGCm'C
Cuts at: 725 2319 2412 3536 725
Size: 1594 93 1124 1883
Fragments arranged by size:
1883 1594 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 306 1096 1906 1987 2322 4466 306
Size: 790 810 81 335 2144 534
Fragments arranged by size:
2144 810 790 534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 365 3061 365
Size: 2696 1998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 517 1045 1210 1408 457
Size: 60 528 165 198 3743
Fragments arranged by size:
3743 528 198 165 60
Bpu10I CC'TnA_GC
Cuts at: 340 929 340
Size: 589 4105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 2749 3463 3761 1870
Size: 879 714 298 2803
Fragments arranged by size:
2803 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 700 4398 700
Size: 3698 996
BsaAI yAC'GTr
Cuts at: 1239 2047 1239
Size: 808 3886
BsaBI GATnn'nnATC
Cuts at: 6 1429 1489 2208 4245 6
Size: 1423 60 719 2037 455
Fragments arranged by size:
2037 1423 719 455 60
BsaHI Gr'CG_yC
Cuts at: 2125 2373 3075 4571 2125
Size: 248 702 1496 2248
Fragments arranged by size:
2248 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1883 2404 3082 3493 3640 3914 1883
Size: 521 678 411 147 274 2663
Fragments arranged by size:
2663 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 427
Size: 4694
BseYI C'CCAG_C
Cuts at: 172 520 1678 2119 2877 3542 3950 172
Size: 348 1158 441 758 665 408 916
Fragments arranged by size:
1158 916 758 665 441 408 348
BsiEI CG_ry'CG
Cuts at: 104 1608 2236 2282 3512 104
Size: 1504 628 46 1230 1286
Fragments arranged by size:
1504 1286 1230 628 46
BsiHKAI G_wGCw'C
Cuts at: 725 1708 2486 2676 3536 725
Size: 983 778 190 860 1883
Fragments arranged by size:
1883 983 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 725 1708 2319 2412 2486 2676 2738 3536 725
Size: 983 611 93 74 190 62 798 1883
Fragments arranged by size:
1883 983 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 989 3126 989
Size: 2137 2557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1673 1744 1958 2260 2641 1673
Size: 71 214 302 381 3726
Fragments arranged by size:
3726 381 302 214 71
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2606 2606
Size: 4694
BsrFI r'CCGG_y
Cuts at: 2692 2873 2692
Size: 181 4513
BssSI C'ACGA_G
Cuts at: 2965 3673 2965
Size: 708 3986
BstAPI GCAn_nnn'nTGC
Cuts at: 1646 2071 1646
Size: 425 4269
BstBI TT'CG_AA
Cuts at: 3055 3055
Size: 4694
BstYI r'GATC_y
Cuts at: 1 306 557 1081 1719 2186 2209 2544 2790
Size: 305 251 524 638 467 23 335 246
Cuts at: 2790 3096 3108 3194 4246 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 638 524 467 449 335 306 305
251 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 690 690
Size: 4694
BtgI C'CryG_G
Cuts at: 1559 1559
Size: 4694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 688 796 1621 2644 2825 2988 688
Size: 108 825 1023 181 163 2394
Fragments arranged by size:
2394 1023 825 181 163 108
BtsI GCAGTG_nn'
Cuts at: 841 1937 841
Size: 1096 3598
DraI TTT'AAA
Cuts at: 741 1693 741
Size: 952 3742
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3744 2400
Size: 1344 3350
EaeI y'GGCC_r
Cuts at: 1670 2279 2453 2844 2871 4216 1670
Size: 609 174 391 27 1345 2148
Fragments arranged by size:
2148 1345 609 391 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 356 799 823 1659 1660 2717 2927 356
Size: 443 24 836 1 1057 210 2123
Fragments arranged by size:
2123 1057 836 443 210 24 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 174 2902 3644 3790 4504 174
Size: 2728 742 146 714 364
Fragments arranged by size:
2728 742 714 364 146
EcoICRI GAG'CTC
Cuts at: 1706 1706
Size: 4694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 517 718 804 1045 1210 1408 1438 2519
Size: 60 201 86 241 165 198 30 1081
Cuts at: 2519 2951 3304 3966 457
Size: 432 353 662 1185
Fragments arranged by size:
1185 1081 662 432 353 241 201 198
165 86 60 30
EcoO109I rG'GnC_Cy
Cuts at: 1005 1833 1005
Size: 828 3866
EcoRI G'AATT_C
Cuts at: 1698 1698
Size: 4694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1200 2713 1200
Size: 1513 3181
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 2128 2376 3606 4377 2128
Size: 248 1230 771 2445
Fragments arranged by size:
2445 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 549 581 2536 2566 2568 2598 472
Size: 32 45 32 1955 30 2 30 2568
Fragments arranged by size:
2568 1955 45 32 32 30 30 2
HincII GTy'rAC
Cuts at: 480 627 1605 1733 480
Size: 147 978 128 3441
Fragments arranged by size:
3441 978 147 128
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 1714 1714
Size: 4694
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3453 3637 3453
Size: 184 4510
MscI TGG'CCA
Cuts at: 1672 2455 1672
Size: 783 3911
MslI CAynn'nnrTG
Cuts at: 112 240 1252 1552 2683 2810 112
Size: 128 1012 300 1131 127 1996
Fragments arranged by size:
1996 1131 1012 300 128 127
MspA1I CmG'CkG
Cuts at: 123 264 1230 1578 1646 1805 2119 2479 3263
Size: 141 966 348 68 159 314 360 784
Cuts at: 3263 3508 123
Size: 245 1309
Fragments arranged by size:
1309 966 784 360 348 314 245 159
141 68
NaeI GCC'GGC
Cuts at: 2875 2875
Size: 4694
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NgoMIV G'CCGG_C
Cuts at: 2873 2873
Size: 4694
NspI r_CATG'y
Cuts at: 348 1257 1747 2247 3850 348
Size: 909 490 500 1603 1192
Fragments arranged by size:
1603 1192 909 500 490
PciI A'CATG_T
Cuts at: 3846 3846
Size: 4694
PflMI CCAn_nnn'nTGG
Cuts at: 395 1209 395
Size: 814 3880
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 4662
PstI C_TGCA'G
Cuts at: 122 306 1741 2426 122
Size: 184 1435 685 2390
Fragments arranged by size:
2390 1435 685 184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 1230 1646 2119 2479 1230
Size: 416 473 360 3445
Fragments arranged by size:
3445 473 416 360
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1708 1708
Size: 4694
SalI G'TCGA_C
Cuts at: 478 1603 1731 478
Size: 1125 128 3441
Fragments arranged by size:
3441 1125 128
SapI GCTCTTCn'nnn_
Cuts at: 1660 2717 2927 1660
Size: 1057 210 3427
Fragments arranged by size:
3427 1057 210
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 4211 4211
Size: 4694
SexAI A'CCwGG_T
Cuts at: 620 620
Size: 4694
SfcI C'TryA_G
Cuts at: 33 118 302 1737 2422 3390 3581 33
Size: 85 184 1435 685 968 191 1146
Fragments arranged by size:
1435 1146 968 685 191 184 85
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1318 1716 1318
Size: 398 4296
SmlI C'TyrA_G
Cuts at: 1358 1849 2764 3478 3740 1358
Size: 491 915 714 262 2312
Fragments arranged by size:
2312 915 714 491 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 348 1257 1747 2247 348
Size: 909 490 500 2795
Fragments arranged by size:
2795 909 500 490
StyI C'CwwG_G
Cuts at: 1001 1175 1509 1828 2182 1001
Size: 174 334 319 354 3513
Fragments arranged by size:
3513 354 334 319 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 1555 1600 2277 2410 2869 3078 1077
Size: 478 45 677 133 459 209 2693
Fragments arranged by size:
2693 677 478 459 209 133 45
TatI w'GTAC_w
Cuts at: 4209 4209
Size: 4694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1073 1105 4211 4243 1073
Size: 32 3106 32 1524
Fragments arranged by size:
3106 1524 32 32
Tth111I GACn'n_nGTC
Cuts at: 475 622 2491 475
Size: 147 1869 2678
Fragments arranged by size:
2678 1869 147
XbaI T'CTAG_A
Cuts at: 1725 1725
Size: 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 541 2189 541
Size: 1648 3046
XhoI C'TCGA_G
Cuts at: 1358 1358
Size: 4694
XmaI C'CCGG_G
Cuts at: 1316 1714 1316
Size: 398 4296
XmnI GAAnn'nnTTC
Cuts at: 445 1096 1367 445
Size: 651 271 3772
Fragments arranged by size:
3772 651 271
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI