Restriction map for
pF1KB8726
(Circular) (Six-base) MAPSORT of: pF1KB8726.seq Check: 5887 from: 1 to: 4187
pF1KB8726 4187 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 878 878
Size: 4187
AccI GT'mk_AC
Cuts at: 1225 1225
Size: 4187
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2444 2797 3459 2012
Size: 432 353 662 2740
Fragments arranged by size:
2740 662 432 353
AfeI AGC'GCT
Cuts at: 586 586
Size: 4187
AflIII A'CryG_T
Cuts at: 1539 3339 3620 1539
Size: 1800 281 2106
Fragments arranged by size:
2106 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1492 2930 1492
Size: 1438 2749
ApaLI G'TGCA_C
Cuts at: 3025 3025
Size: 4187
ApoI r'AATT_y
Cuts at: 1191 3540 3796 1191
Size: 2349 256 1582
Fragments arranged by size:
2349 1582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 281 403 658 1087 1207 281
Size: 122 255 429 120 3261
Fragments arranged by size:
3261 429 255 122 120
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 1083 1203 1617 1865 1900 1083
Size: 120 414 248 35 3370
Fragments arranged by size:
3370 414 248 120 35
BanII G_rGCy'C
Cuts at: 429 1201 2231 429
Size: 772 1030 2385
Fragments arranged by size:
2385 1030 772
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BbsI GAAGACnn'nnnn_
Cuts at: 941 941
Size: 4187
BbvCI CC'TCA_GC
Cuts at: 619 907 1160 619
Size: 288 253 3646
Fragments arranged by size:
3646 288 253
BciVI GTATCCnnnnn_n'
Cuts at: 796 2085 3141 4187 796
Size: 1289 1056 1046 796
Fragments arranged by size:
1289 1056 1046 796
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglII A'GATC_T
Cuts at: 338 1702 3739 338
Size: 1364 2037 786
Fragments arranged by size:
2037 1364 786
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 1088 1812 1905 3029 1088
Size: 724 93 1124 2246
Fragments arranged by size:
2246 1124 724 93
BmgBI CAC'GTC
Cuts at: 441 947 441
Size: 506 3681
BmrI ACTGGGnnnn_n'
Cuts at: 1399 1480 1815 3959 1399
Size: 81 335 2144 1627
Fragments arranged by size:
2144 1627 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1113 726
Size: 387 3800
Bpu10I CC'TnA_GC
Cuts at: 619 907 1160 619
Size: 288 253 3646
Fragments arranged by size:
3646 288 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 502 1041 1363 2242 2956 3254 193
Size: 309 539 322 879 714 298 1126
Fragments arranged by size:
1126 879 714 539 322 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 1117 3891 340
Size: 777 2774 636
Fragments arranged by size:
2774 777 636
BsaAI yAC'GTr
Cuts at: 1540 1540
Size: 4187
BsaBI GATnn'nnATC
Cuts at: 6 292 367 1701 3738 6
Size: 286 75 1334 2037 455
Fragments arranged by size:
2037 1334 455 286 75
BsaHI Gr'CG_yC
Cuts at: 386 875 1618 1866 2568 4064 386
Size: 489 743 248 702 1496 509
Fragments arranged by size:
1496 743 702 509 489 248
BsaWI w'CCGG_w
Cuts at: 1153 1376 1897 2575 2986 3133 3407 1153
Size: 223 521 678 411 147 274 1933
Fragments arranged by size:
1933 678 521 411 274 223 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 967 997 1148 1178 301
Size: 30 636 30 151 30 3310
Fragments arranged by size:
3310 636 151 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 459 526 391
Size: 68 67 4052
Fragments arranged by size:
4052 68 67
BseYI C'CCAG_C
Cuts at: 1064 1612 2370 3035 3443 1064
Size: 548 758 665 408 1808
Fragments arranged by size:
1808 758 665 548 408
BsiEI CG_ry'CG
Cuts at: 104 240 1729 1775 3005 104
Size: 136 1489 46 1230 1286
Fragments arranged by size:
1489 1286 1230 136 46
BsiHKAI G_wGCw'C
Cuts at: 800 1201 1979 2169 3029 800
Size: 401 778 190 860 1958
Fragments arranged by size:
1958 860 778 401 190
Bsp1286I G_dGCh'C
Cuts at: 429 800 1088 1201 1812 1905 1979 2169 2231
Size: 371 288 113 611 93 74 190 62
Cuts at: 2231 3029 429
Size: 798 1587
Fragments arranged by size:
1587 798 611 371 288 190 113 93
74 62
BspEI T'CCGG_A
Cuts at: 1153 1153
Size: 4187
BspHI T'CATG_A
Cuts at: 2619 2619
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1237 1451 1753 2134 841
Size: 396 214 302 381 2894
Fragments arranged by size:
2894 396 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 609 2099 609
Size: 1490 2697
BsrFI r'CCGG_y
Cuts at: 1034 1070 2185 2366 1034
Size: 36 1115 181 2855
Fragments arranged by size:
2855 1115 181 36
BsrGI T'GTAC_A
Cuts at: 725 725
Size: 4187
BssSI C'ACGA_G
Cuts at: 915 2458 3166 915
Size: 1543 708 1936
Fragments arranged by size:
1936 1543 708
BstAPI GCAn_nnn'nTGC
Cuts at: 649 1074 1564 649
Size: 425 490 3272
Fragments arranged by size:
3272 490 425
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 1051 1051
Size: 4187
BstYI r'GATC_y
Cuts at: 1 338 362 902 1212 1679 1702 2037 2283
Size: 337 24 540 310 467 23 335 246
Cuts at: 2283 2589 2601 2687 3739 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 540 467 449 337 335 310 306
246 86 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 127 127
Size: 4187
BtgI C'CryG_G
Cuts at: 107 312 107
Size: 205 3982
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
ClaI AT'CG_AT
Cuts at: 896 896
Size: 4187
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DraIII CAC_nnn'GTG
Cuts at: 804 804
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 3237 1893
Size: 1344 2843
EaeI y'GGCC_r
Cuts at: 1118 1772 1946 2337 2364 3709 1118
Size: 654 174 391 27 1345 1596
Fragments arranged by size:
1596 1345 654 391 174 27
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 462 2395 3137 3283 3997 462
Size: 1933 742 146 714 652
Fragments arranged by size:
1933 742 714 652 146
EcoICRI GAG'CTC
Cuts at: 1199 1199
Size: 4187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1113 2012 2444 2797 3459 726
Size: 387 899 432 353 662 1454
Fragments arranged by size:
1454 899 662 432 387 353
EcoNI CCTnn'n_nnAGG
Cuts at: 433 541 963 433
Size: 108 422 3657
Fragments arranged by size:
3657 422 108
EcoO109I rG'GnC_Cy
Cuts at: 513 846 1326 513
Size: 333 480 3374
Fragments arranged by size:
3374 480 333
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1035 2206 1035
Size: 1171 3016
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 207 588 1621 1869 3099 3870 207
Size: 381 1033 248 1230 771 524
Fragments arranged by size:
1230 1033 771 524 381 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2029 2059 2061 2091 2029
Size: 30 2 30 4125
Fragments arranged by size:
4125 30 30 2
HincII GTy'rAC
Cuts at: 309 1226 309
Size: 917 3270
HindIII A'AGCT_T
Cuts at: 1048 1048
Size: 4187
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 483 788 2946 3130 483
Size: 305 2158 184 1540
Fragments arranged by size:
2158 1540 305 184
MscI TGG'CCA
Cuts at: 1948 1948
Size: 4187
MslI CAynn'nnrTG
Cuts at: 373 896 2176 2303 373
Size: 523 1280 127 2257
Fragments arranged by size:
2257 1280 523 127
MspA1I CmG'CkG
Cuts at: 885 1298 1612 1972 2756 3001 885
Size: 413 314 360 784 245 2071
Fragments arranged by size:
2071 784 413 360 314 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 4187
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 107 107
Size: 4187
NdeI CA'TA_TG
Cuts at: 648 648
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 4187
NspI r_CATG'y
Cuts at: 864 1240 1740 3343 864
Size: 376 500 1603 1708
Fragments arranged by size:
1708 1603 500 376
PciI A'CATG_T
Cuts at: 3339 3339
Size: 4187
PflMI CCAn_nnn'nTGG
Cuts at: 495 495
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 4155
PpuMI rG'GwC_Cy
Cuts at: 513 846 513
Size: 333 3854
PspXI vC'TCGA_Gb
Cuts at: 403 403
Size: 4187
PstI C_TGCA'G
Cuts at: 671 869 926 1234 1919 671
Size: 198 57 308 685 2939
Fragments arranged by size:
2939 685 308 198 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 1612 1972 1612
Size: 360 3827
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 1201 1201
Size: 4187
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 484 3704 484
Size: 3220 967
SfcI C'TryA_G
Cuts at: 33 667 865 922 1230 1915 2883 3074 33
Size: 634 198 57 308 685 968 191 1146
Fragments arranged by size:
1146 968 685 634 308 198 191 57
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 208 403 517 1020 1342 2257 2971 3233 208
Size: 195 114 503 322 915 714 262 1162
Fragments arranged by size:
1162 915 714 503 322 262 195 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 864 1240 1740 864
Size: 376 500 3311
Fragments arranged by size:
3311 500 376
StyI C'CwwG_G
Cuts at: 107 689 1321 1675 107
Size: 582 632 354 2619
Fragments arranged by size:
2619 632 582 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 1903 2362 2571 1770
Size: 133 459 209 3386
Fragments arranged by size:
3386 459 209 133
TatI w'GTAC_w
Cuts at: 482 725 3702 482
Size: 243 2977 967
Fragments arranged by size:
2977 967 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 505 537 1148 1180 3704 3736 505
Size: 32 611 32 2524 32 956
Fragments arranged by size:
2524 956 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 502 1984 502
Size: 1482 2705
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1682 1682
Size: 4187
XhoI C'TCGA_G
Cuts at: 403 403
Size: 4187
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
ZraI GAC'GTC
Cuts at: 876 876
Size: 4187
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I CspCI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI