Restriction map for
pF1KB8727
(Circular) (Six-base) MAPSORT of: pF1KB8727.seq Check: 3872 from: 1 to: 4589
pF1KB8727 4589 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1188 1627 1188
Size: 439 4150
Acc65I G'GTAC_C
Cuts at: 1605 1605
Size: 4589
AclI AA'CG_TT
Cuts at: 4429 4429
Size: 4589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2846 3199 3861 2414
Size: 432 353 662 3142
Fragments arranged by size:
3142 662 432 353
AflIII A'CryG_T
Cuts at: 485 1208 1941 3741 4022 485
Size: 723 733 1800 281 1052
Fragments arranged by size:
1800 1052 733 723 281
AhdI GACnn_n'nnGTC
Cuts at: 582 582
Size: 4589
AleI CACnn'nnGTG
Cuts at: 442 1387 442
Size: 945 3644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1126 1158 1126
Size: 32 4557
AlwNI CAG_nnn'CTG
Cuts at: 1173 1377 1894 3332 1173
Size: 204 517 1438 2430
Fragments arranged by size:
2430 1438 517 204
ApaI G_GGCC'C
Cuts at: 201 1087 1510 201
Size: 886 423 3280
Fragments arranged by size:
3280 886 423
ApaLI G'TGCA_C
Cuts at: 3427 3427
Size: 4589
ApoI r'AATT_y
Cuts at: 1432 1441 1593 3942 4198 1432
Size: 9 152 2349 256 1823
Fragments arranged by size:
2349 1823 256 152 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 4589
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
AvaI C'yCGr_G
Cuts at: 1512 1609 1512
Size: 97 4492
BamHI G'GATC_C
Cuts at: 1614 1614
Size: 4589
BanI G'GyrC_C
Cuts at: 353 831 1605 2019 2267 2302 353
Size: 478 774 414 248 35 2640
Fragments arranged by size:
2640 774 478 414 248 35
BanII G_rGCy'C
Cuts at: 201 851 878 920 1087 1144 1302 1510 1603
Size: 650 27 42 167 57 158 208 93
Cuts at: 1603 2633 201
Size: 1030 2157
Fragments arranged by size:
2157 1030 650 208 167 158 93 57
42 27
BbeI G_GCGC'C
Cuts at: 2023 2271 2023
Size: 248 4341
BbsI GAAGACnn'nnnn_
Cuts at: 1552 1552
Size: 4589
BbvCI CC'TCA_GC
Cuts at: 1497 1502 1497
Size: 5 4584
BciVI GTATCCnnnnn_n'
Cuts at: 2487 3543 4589 2487
Size: 1056 1046 2487
Fragments arranged by size:
2487 1056 1046
BclI T'GATC_A
Cuts at: 2109 2109
Size: 4589
BglI GCCn_nnn'nGGC
Cuts at: 1060 1060
Size: 4589
BglII A'GATC_T
Cuts at: 212 2104 4141 212
Size: 1892 2037 660
Fragments arranged by size:
2037 1892 660
BlpI GC'TnA_GC
Cuts at: 1701 1701
Size: 4589
Bme1580I G_kGCm'C
Cuts at: 201 356 655 836 1087 1510 2214 2307 3431
Size: 155 299 181 251 423 704 93 1124
Cuts at: 3431 201
Size: 1359
Fragments arranged by size:
1359 1124 704 423 299 251 181 155
93
BmgBI CAC'GTC
Cuts at: 1389 1389
Size: 4589
BmrI ACTGGGnnnn_n'
Cuts at: 204 1330 1801 1882 2217 4361 204
Size: 1126 471 81 335 2144 432
Fragments arranged by size:
2144 1126 471 432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 574 645 799 880 934 1128 1195 1316
Size: 449 71 154 81 54 194 67 121
Cuts at: 1316 1473 125
Size: 157 3241
Fragments arranged by size:
3241 449 194 157 154 121 81 71
67 54
Bpu10I CC'TnA_GC
Cuts at: 1497 1502 1497
Size: 5 4584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1765 2644 3358 3656 1765
Size: 879 714 298 2698
Fragments arranged by size:
2698 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4293 4293
Size: 4589
BsaAI yAC'GTr
Cuts at: 366 887 1942 366
Size: 521 1055 3013
Fragments arranged by size:
3013 1055 521
BsaBI GATnn'nnATC
Cuts at: 6 2103 4140 6
Size: 2097 2037 455
Fragments arranged by size:
2097 2037 455
BsaHI Gr'CG_yC
Cuts at: 1307 2020 2268 2970 4466 1307
Size: 713 248 702 1496 1430
Fragments arranged by size:
1496 1430 713 702 248
BsaWI w'CCGG_w
Cuts at: 721 1778 2299 2977 3388 3535 3809 721
Size: 1057 521 678 411 147 274 1501
Fragments arranged by size:
1501 1057 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1126 1156 1126
Size: 30 4559
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 967 967
Size: 4589
BseYI C'CCAG_C
Cuts at: 1092 1118 1530 2014 2772 3437 3845 1092
Size: 26 412 484 758 665 408 1836
Fragments arranged by size:
1836 758 665 484 412 408 26
BsiEI CG_ry'CG
Cuts at: 104 2131 2177 3407 104
Size: 2027 46 1230 1286
Fragments arranged by size:
2027 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 461 878 920 1324 1603 2381 2571 3431 461
Size: 417 42 404 279 778 190 860 1619
Fragments arranged by size:
1619 860 778 417 404 279 190 42
BsmBI CGTCTCn'nnnn_
Cuts at: 226 226
Size: 4589
Bsp1286I G_dGCh'C
Cuts at: 201 356 461 655 836 851 878 920 1087
Size: 155 105 194 181 15 27 42 167
Cuts at: 1087 1144 1302 1324 1510 1603 2214 2307 2381
Size: 57 158 22 186 93 611 93 74
Cuts at: 2381 2571 2633 3431 201
Size: 190 62 798 1359
Fragments arranged by size:
1359 798 611 194 190 186 181 167
158 155 105 93 93 74 62 57
42 27 22 15
BspHI T'CATG_A
Cuts at: 3021 3021
Size: 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 1639 1853 2155 2536 545
Size: 1094 214 302 381 2598
Fragments arranged by size:
2598 1094 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2881 2935 4419 4584 50
Size: 2831 54 1484 165 55
Fragments arranged by size:
2831 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2501 2501
Size: 4589
BsrFI r'CCGG_y
Cuts at: 1059 1102 2587 2768 1059
Size: 43 1485 181 2880
Fragments arranged by size:
2880 1485 181 43
BssSI C'ACGA_G
Cuts at: 301 2860 3568 301
Size: 2559 708 1322
Fragments arranged by size:
2559 1322 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1966 1966
Size: 4589
BstBI TT'CG_AA
Cuts at: 2950 2950
Size: 4589
BstEII G'GTnAC_C
Cuts at: 519 1419 519
Size: 900 3689
BstXI CCAn_nnnn'nTGG
Cuts at: 1389 1389
Size: 4589
BstYI r'GATC_y
Cuts at: 1 212 1614 2081 2104 2439 2685 2991 3003
Size: 211 1402 467 23 335 246 306 12
Cuts at: 3003 3089 4141 1
Size: 86 1052 449
Fragments arranged by size:
1402 1052 467 449 335 306 246 211
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1412 1412
Size: 4589
BtgI C'CryG_G
Cuts at: 107 1466 107
Size: 1359 3230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2539 2720 2883 2539
Size: 181 163 4245
Fragments arranged by size:
4245 181 163
BtsI GCAGTG_nn'
Cuts at: 777 1155 1378 1832 777
Size: 378 223 454 3534
Fragments arranged by size:
3534 454 378 223
DraI TTT'AAA
Cuts at: 1588 1588
Size: 4589
DraIII CAC_nnn'GTG
Cuts at: 306 306
Size: 4589
DrdI GACnn_nn'nnGTC
Cuts at: 2295 3639 2295
Size: 1344 3245
EaeI y'GGCC_r
Cuts at: 374 681 2174 2348 2739 2766 4111 374
Size: 307 1493 174 391 27 1345 852
Fragments arranged by size:
1493 1345 852 391 307 174 27
EagI C'GGCC_G
Cuts at: 2174 2174
Size: 4589
EarI CTCTTCn'nnn_
Cuts at: 268 1400 2612 2822 268
Size: 1132 1212 210 2035
Fragments arranged by size:
2035 1212 1132 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2797 3539 3685 4399 2797
Size: 742 146 714 2987
Fragments arranged by size:
2987 742 714 146
EcoICRI GAG'CTC
Cuts at: 876 918 1601 876
Size: 42 683 3864
Fragments arranged by size:
3864 683 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 574 645 799 880 934 1128 1195 1316
Size: 449 71 154 81 54 194 67 121
Cuts at: 1316 1473 2414 2846 3199 3861 125
Size: 157 941 432 353 662 853
Fragments arranged by size:
941 853 662 449 432 353 194 157
154 121 81 71 67 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1357 1357
Size: 4589
EcoO109I rG'GnC_Cy
Cuts at: 198 244 534 909 1041 1084 1506 1507 1516
Size: 46 290 375 132 43 422 1 9
Cuts at: 1516 1728 198
Size: 212 3059
Fragments arranged by size:
3059 422 375 290 212 132 46 43
9 1
EcoRI G'AATT_C
Cuts at: 1593 1593
Size: 4589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4589
FspI TGC'GCA
Cuts at: 769 2370 769
Size: 1601 2988
HaeII r_GCGC'y
Cuts at: 1537 2023 2271 3501 4272 1537
Size: 486 248 1230 771 1854
Fragments arranged by size:
1854 1230 771 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 140 172 300 332 568 600 616 648 2431
Size: 32 128 32 236 32 16 32 1783
Cuts at: 2431 2461 2463 2493 140
Size: 30 2 30 2236
Fragments arranged by size:
2236 1783 236 128 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 1189 1628 1189
Size: 439 4150
KasI G'GCGC_C
Cuts at: 2019 2267 2019
Size: 248 4341
KpnI G_GTAC'C
Cuts at: 1609 1609
Size: 4589
MluI A'CGCG_T
Cuts at: 485 4022 485
Size: 3537 1052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 3348 3532 791
Size: 2557 184 1848
Fragments arranged by size:
2557 1848 184
MscI TGG'CCA
Cuts at: 376 683 2350 376
Size: 307 1667 2615
Fragments arranged by size:
2615 1667 307
MslI CAynn'nnrTG
Cuts at: 442 1387 2578 2705 442
Size: 945 1191 127 2326
Fragments arranged by size:
2326 1191 945 127
MspA1I CmG'CkG
Cuts at: 906 945 1066 1122 1468 1501 1700 2014 2374
Size: 39 121 56 346 33 199 314 360
Cuts at: 2374 3158 3403 906
Size: 784 245 2092
Fragments arranged by size:
2092 784 360 346 314 245 199 121
56 39 33
NaeI GCC'GGC
Cuts at: 1061 2770 1061
Size: 1709 2880
NarI GG'CG_CC
Cuts at: 2020 2268 2020
Size: 248 4341
NcoI C'CATG_G
Cuts at: 107 107
Size: 4589
NgoMIV G'CCGG_C
Cuts at: 1059 2768 1059
Size: 1709 2880
NspI r_CATG'y
Cuts at: 582 691 1212 1572 1642 2142 3745 582
Size: 109 521 360 70 500 1603 1426
Fragments arranged by size:
1603 1426 521 500 360 109 70
PciI A'CATG_T
Cuts at: 1208 3741 1208
Size: 2533 2056
PflMI CCAn_nnn'nTGG
Cuts at: 369 369
Size: 4589
PfoI T'CCnGG_A
Cuts at: 911 1445 911
Size: 534 4055
PmeI GTTT'AAAC
Cuts at: 1588 1588
Size: 4589
PmlI CAC'GTG
Cuts at: 366 887 366
Size: 521 4068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1126 1158 3035 3067 1126
Size: 32 1877 32 2648
Fragments arranged by size:
2648 1877 32 32
PpuMI rG'GwC_Cy
Cuts at: 244 534 909 1516 244
Size: 290 375 607 3317
Fragments arranged by size:
3317 607 375 290
PspOMI G'GGCC_C
Cuts at: 197 1083 1506 197
Size: 886 423 3280
Fragments arranged by size:
3280 886 423
PstI C_TGCA'G
Cuts at: 185 872 1169 1636 2321 185
Size: 687 297 467 685 2453
Fragments arranged by size:
2453 687 685 467 297
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4589
PvuII CAG'CTG
Cuts at: 945 1066 1122 1501 2014 2374 945
Size: 121 56 379 513 360 3160
Fragments arranged by size:
3160 513 379 360 121 56
RsrII CG'GwC_CG
Cuts at: 1470 2784 1470
Size: 1314 3275
SacI G_AGCT'C
Cuts at: 878 920 1603 878
Size: 42 683 3864
Fragments arranged by size:
3864 683 42
SacII CC_GC'GG
Cuts at: 1469 1469
Size: 4589
SalI G'TCGA_C
Cuts at: 1187 1626 1187
Size: 439 4150
SanDI GG'GwC_CC
Cuts at: 1516 1516
Size: 4589
SapI GCTCTTCn'nnn_
Cuts at: 1400 2612 2822 1400
Size: 1212 210 3167
Fragments arranged by size:
3167 1212 210
SbfI CC_TGCA'GG
Cuts at: 1636 1636
Size: 4589
ScaI AGT'ACT
Cuts at: 4106 4106
Size: 4589
SfcI C'TryA_G
Cuts at: 33 181 868 1165 1632 2317 3285 3476 33
Size: 148 687 297 467 685 968 191 1146
Fragments arranged by size:
1146 968 687 685 467 297 191 148
SfoI GGC'GCC
Cuts at: 2021 2269 2021
Size: 248 4341
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
SmaI CCC'GGG
Cuts at: 1514 1611 1514
Size: 97 4492
SmlI C'TyrA_G
Cuts at: 1744 2659 3373 3635 1744
Size: 915 714 262 2698
Fragments arranged by size:
2698 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4589
SphI G_CATG'C
Cuts at: 691 1642 2142 691
Size: 951 500 3138
Fragments arranged by size:
3138 951 500
StuI AGG'CCT
Cuts at: 551 1180 1348 551
Size: 629 168 3792
Fragments arranged by size:
3792 629 168
StyI C'CwwG_G
Cuts at: 107 1037 1723 2077 107
Size: 930 686 354 2619
Fragments arranged by size:
2619 930 686 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 450 603 853 1357 1487 2172 2305 2764 2973
Size: 153 250 504 130 685 133 459 209
Cuts at: 2973 450
Size: 2066
Fragments arranged by size:
2066 685 504 459 250 209 153 133
130
TatI w'GTAC_w
Cuts at: 4104 4104
Size: 4589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4106 4138 4106
Size: 32 4557
Tth111I GACn'n_nGTC
Cuts at: 2386 2386
Size: 4589
XbaI T'CTAG_A
Cuts at: 1620 1620
Size: 4589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 570 2084 570
Size: 1514 3075
XmaI C'CCGG_G
Cuts at: 1512 1609 1512
Size: 97 4492
XmnI GAAnn'nnTTC
Cuts at: 130 130
Size: 4589
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AscI AvrII
BaeI BcgI BfrBI BmtI BsgI BsiWI BsmI BspEI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI