Restriction map for
pF1KB8730
(Circular) (Six-base) MAPSORT of: pF1KB8730.seq Check: 725 from: 1 to: 4292
pF1KB8730 4292 bp
With 182 enzymes: SgfI *
April 2, 2009 17:32 ..
AccI GT'mk_AC
Cuts at: 1234 1330 1234
Size: 96 4196
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 197 4132 197
Size: 3935 357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1081 1234 2117 2549 2902 3564 293
Size: 788 153 883 432 353 662 1021
Fragments arranged by size:
1021 883 788 662 432 353 153
AfeI AGC'GCT
Cuts at: 649 1117 649
Size: 468 3824
AflIII A'CryG_T
Cuts at: 815 1644 3444 3725 815
Size: 829 1800 281 1382
Fragments arranged by size:
1800 1382 829 281
AlwNI CAG_nnn'CTG
Cuts at: 313 830 1597 3035 313
Size: 517 767 1438 1570
Fragments arranged by size:
1570 1438 767 517
ApaLI G'TGCA_C
Cuts at: 3130 3130
Size: 4292
ApoI r'AATT_y
Cuts at: 705 1296 3645 3901 705
Size: 591 2349 256 1096
Fragments arranged by size:
2349 1096 591 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 1312 1312
Size: 4292
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 568 759 1308 1722 1970 2005 568
Size: 191 549 414 248 35 2855
Fragments arranged by size:
2855 549 414 248 191 35
BanII G_rGCy'C
Cuts at: 143 1200 1306 2336 143
Size: 1057 106 1030 2099
Fragments arranged by size:
2099 1057 1030 106
BbeI G_GCGC'C
Cuts at: 1726 1974 1726
Size: 248 4044
BbsI GAAGACnn'nnnn_
Cuts at: 281 887 281
Size: 606 3686
BbvCI CC'TCA_GC
Cuts at: 680 680
Size: 4292
BciVI GTATCCnnnnn_n'
Cuts at: 2190 3246 4292 2190
Size: 1056 1046 2190
Fragments arranged by size:
2190 1056 1046
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglII A'GATC_T
Cuts at: 1807 3844 1807
Size: 2037 2255
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 764 1917 2010 3134 764
Size: 1153 93 1124 1922
Fragments arranged by size:
1922 1153 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 910 1504 1585 1920 4064 910
Size: 594 81 335 2144 1138
Fragments arranged by size:
2144 1138 594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 669 801 1214 2659 669
Size: 132 413 1445 2302
Fragments arranged by size:
2302 1445 413 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 1188 870
Size: 318 3974
Bpu10I CC'TnA_GC
Cuts at: 680 680
Size: 4292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1468 2347 3061 3359 1141
Size: 327 879 714 298 2074
Fragments arranged by size:
2074 879 714 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 941 3996 941
Size: 3055 1237
BsaAI yAC'GTr
Cuts at: 482 1645 482
Size: 1163 3129
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 1723 1971 2673 4169 1723
Size: 248 702 1496 1846
Fragments arranged by size:
1846 1496 702 248
BsaWI w'CCGG_w
Cuts at: 848 1481 2002 2680 3091 3238 3512 848
Size: 633 521 678 411 147 274 1628
Fragments arranged by size:
1628 678 633 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 1030 1060 106
Size: 30 894 30 3338
Fragments arranged by size:
3338 894 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 668 1038 127
Size: 541 370 3381
Fragments arranged by size:
3381 541 370
BseYI C'CCAG_C
Cuts at: 1055 1171 1717 2475 3140 3548 1055
Size: 116 546 758 665 408 1799
Fragments arranged by size:
1799 758 665 546 408 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 347 556 317
Size: 30 209 4053
Fragments arranged by size:
4053 209 30
BsiEI CG_ry'CG
Cuts at: 104 1834 1880 3110 104
Size: 1730 46 1230 1286
Fragments arranged by size:
1730 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1018 1029 1306 2084 2274 3134 1018
Size: 11 277 778 190 860 2176
Fragments arranged by size:
2176 860 778 277 190 11
BsmI GAATG_Cn'
Cuts at: 308 450 308
Size: 142 4150
Bsp1286I G_dGCh'C
Cuts at: 143 764 1018 1029 1200 1306 1917 2010 2084
Size: 621 254 11 171 106 611 93 74
Cuts at: 2084 2274 2336 3134 143
Size: 190 62 798 1301
Fragments arranged by size:
1301 798 621 611 254 190 171 106
93 74 62 11
BspHI T'CATG_A
Cuts at: 2724 2724
Size: 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 1342 1556 1858 2239 535
Size: 807 214 302 381 2588
Fragments arranged by size:
2588 807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2584 2638 4122 4287 50
Size: 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 230 2204 230
Size: 1974 2318
BsrFI r'CCGG_y
Cuts at: 351 950 2290 2471 351
Size: 599 1340 181 2172
Fragments arranged by size:
2172 1340 599 181
BssSI C'ACGA_G
Cuts at: 997 2563 3271 997
Size: 1566 708 2018
Fragments arranged by size:
2018 1566 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 1669
Size: 4292
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstYI r'GATC_y
Cuts at: 1 126 1100 1317 1784 1807 2142 2388 2694
Size: 125 974 217 467 23 335 246 306
Cuts at: 2694 2706 2792 3844 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 974 467 449 335 306 246 217
125 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 776 776
Size: 4292
BtgI C'CryG_G
Cuts at: 107 584 1104 107
Size: 477 520 3295
Fragments arranged by size:
3295 520 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 677 2242 2423 2586 677
Size: 1565 181 163 2383
Fragments arranged by size:
2383 1565 181 163
BtsI GCAGTG_nn'
Cuts at: 1027 1535 1027
Size: 508 3784
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 465 1998 3342 465
Size: 1533 1344 1415
Fragments arranged by size:
1533 1415 1344
EaeI y'GGCC_r
Cuts at: 518 587 1877 2051 2442 2469 3814 518
Size: 69 1290 174 391 27 1345 996
Fragments arranged by size:
1345 1290 996 391 174 69 27
EagI C'GGCC_G
Cuts at: 1877 1877
Size: 4292
EarI CTCTTCn'nnn_
Cuts at: 959 2315 2525 959
Size: 1356 210 2726
Fragments arranged by size:
2726 1356 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2500 3242 3388 4102 2500
Size: 742 146 714 2690
Fragments arranged by size:
2690 742 714 146
EcoICRI GAG'CTC
Cuts at: 1304 1304
Size: 4292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 870 1081 1188 1234 2117 2549 2902 3564
Size: 577 211 107 46 883 432 353 662
Cuts at: 3564 293
Size: 1021
Fragments arranged by size:
1021 883 662 577 432 353 211 107
46
EcoO109I rG'GnC_Cy
Cuts at: 1273 1431 1273
Size: 158 4134
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 4292
FspI TGC'GCA
Cuts at: 2073 2073
Size: 4292
HaeII r_GCGC'y
Cuts at: 633 651 1119 1726 1974 3204 3975 633
Size: 18 468 607 248 1230 771 950
Fragments arranged by size:
1230 950 771 607 468 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 2134 2164 2166 2196 106
Size: 32 1996 30 2 30 2202
Fragments arranged by size:
2202 1996 32 30 30 2
HincII GTy'rAC
Cuts at: 1331 1331
Size: 4292
KasI G'GCGC_C
Cuts at: 1722 1970 1722
Size: 248 4044
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3051 3235 3051
Size: 184 4108
MscI TGG'CCA
Cuts at: 520 589 2053 520
Size: 69 1464 2759
Fragments arranged by size:
2759 1464 69
MslI CAynn'nnrTG
Cuts at: 2281 2408 2281
Size: 127 4165
MspA1I CmG'CkG
Cuts at: 540 1403 1717 2077 2861 3106 540
Size: 863 314 360 784 245 1726
Fragments arranged by size:
1726 863 784 360 314 245
NaeI GCC'GGC
Cuts at: 2473 2473
Size: 4292
NarI GG'CG_CC
Cuts at: 1723 1971 1723
Size: 248 4044
NcoI C'CATG_G
Cuts at: 107 584 107
Size: 477 3815
NgoMIV G'CCGG_C
Cuts at: 2471 2471
Size: 4292
NspI r_CATG'y
Cuts at: 819 1345 1845 3448 819
Size: 526 500 1603 1663
Fragments arranged by size:
1663 1603 526 500
PasI CC'CwG_GG
Cuts at: 1009 1009
Size: 4292
PciI A'CATG_T
Cuts at: 815 3444 815
Size: 2629 1663
PflMI CCAn_nnn'nTGG
Cuts at: 225 1078 225
Size: 853 3439
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 4260
PshAI GACnn'nnGTC
Cuts at: 281 281
Size: 4292
PstI C_TGCA'G
Cuts at: 1339 2024 1339
Size: 685 3607
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 540 1717 2077 540
Size: 1177 360 2755
Fragments arranged by size:
2755 1177 360
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 1306 1306
Size: 4292
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 1335 2020 2988 3179 33
Size: 1302 685 968 191 1146
Fragments arranged by size:
1302 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1724 1972 1724
Size: 248 4044
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SgrAI Cr'CCGG_yG
Cuts at: 351 351
Size: 4292
SmaI CCC'GGG
Cuts at: 1314 1314
Size: 4292
SmlI C'TyrA_G
Cuts at: 1156 1447 2362 3076 3338 1156
Size: 291 915 714 262 2110
Fragments arranged by size:
2110 915 714 291 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
SspI AAT'ATT
Cuts at: 1087 1087
Size: 4292
StuI AGG'CCT
Cuts at: 1147 1147
Size: 4292
StyI C'CwwG_G
Cuts at: 107 584 1148 1426 1780 107
Size: 477 564 278 354 2619
Fragments arranged by size:
2619 564 477 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1875 2008 2467 2676 1875
Size: 133 459 209 3491
Fragments arranged by size:
3491 459 209 133
TatI w'GTAC_w
Cuts at: 3807 3807
Size: 4292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1201 1233 3809 3841 1201
Size: 32 2576 32 1652
Fragments arranged by size:
2576 1652 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 374 873 1787 374
Size: 499 914 2879
Fragments arranged by size:
2879 914 499
XmaI C'CCGG_G
Cuts at: 1312 1312
Size: 4292
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SwaI XhoI
XmnI ZraI