Restriction map for
pF1KB8731
(Circular) (Six-base) MAPSORT of: pF1KB8731.seq Check: 9513 from: 1 to: 5171
pF1KB8731 5171 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2209 2209
Size: 5171
Acc65I G'GTAC_C
Cuts at: 2187 2187
Size: 5171
AclI AA'CG_TT
Cuts at: 5011 5011
Size: 5171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 759 927 1347 1599 2996 3428 3781 4443
Size: 307 168 420 252 1397 432 353 662
Cuts at: 4443 452
Size: 1180
Fragments arranged by size:
1397 1180 662 432 420 353 307 252
168
AflIII A'CryG_T
Cuts at: 1221 2523 4323 4604 1221
Size: 1302 1800 281 1788
Fragments arranged by size:
1800 1788 1302 281
AleI CACnn'nnGTG
Cuts at: 1322 1826 1322
Size: 504 4667
AlwNI CAG_nnn'CTG
Cuts at: 2476 3914 2476
Size: 1438 3733
ApaLI G'TGCA_C
Cuts at: 1891 4009 1891
Size: 2118 3053
ApoI r'AATT_y
Cuts at: 412 460 725 809 893 977 1229 1397 1481
Size: 48 265 84 84 84 252 168 84
Cuts at: 1481 1565 2153 2175 4524 4780 412
Size: 84 588 22 2349 256 803
Fragments arranged by size:
2349 803 588 265 256 252 168 84
84 84 84 84 48 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 5171
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5171
AvaI C'yCGr_G
Cuts at: 2191 2191
Size: 5171
BamHI G'GATC_C
Cuts at: 183 2196 183
Size: 2013 3158
BanI G'GyrC_C
Cuts at: 2187 2601 2849 2884 2187
Size: 414 248 35 4474
Fragments arranged by size:
4474 414 248 35
BanII G_rGCy'C
Cuts at: 2185 3215 2185
Size: 1030 4141
BbeI G_GCGC'C
Cuts at: 2605 2853 2605
Size: 248 4923
BciVI GTATCCnnnnn_n'
Cuts at: 3069 4125 5171 3069
Size: 1056 1046 3069
Fragments arranged by size:
3069 1056 1046
BclI T'GATC_A
Cuts at: 966 2691 966
Size: 1725 3446
BfrBI ATG'CAT
Cuts at: 919 1087 919
Size: 168 5003
BglII A'GATC_T
Cuts at: 2686 4723 2686
Size: 2037 3134
BlpI GC'TnA_GC
Cuts at: 1290 2283 1290
Size: 993 4178
Bme1580I G_kGCm'C
Cuts at: 1895 2796 2889 4013 1895
Size: 901 93 1124 3053
Fragments arranged by size:
3053 1124 901 93
BmrI ACTGGGnnnn_n'
Cuts at: 1079 1499 2383 2464 2799 4943 1079
Size: 420 884 81 335 2144 1307
Fragments arranged by size:
2144 1307 884 420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 5171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 923 1007 1595 1679 755
Size: 168 84 588 84 4247
Fragments arranged by size:
4247 588 168 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2347 3226 3940 4238 2347
Size: 879 714 298 3280
Fragments arranged by size:
3280 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4875 4875
Size: 5171
BsaAI yAC'GTr
Cuts at: 2524 2524
Size: 5171
BsaBI GATnn'nnATC
Cuts at: 6 368 2685 4722 6
Size: 362 2317 2037 455
Fragments arranged by size:
2317 2037 455 362
BsaHI Gr'CG_yC
Cuts at: 2602 2850 3552 5048 2602
Size: 248 702 1496 2725
Fragments arranged by size:
2725 1496 702 248
BsaWI w'CCGG_w
Cuts at: 206 2360 2881 3559 3970 4117 4391 206
Size: 2154 521 678 411 147 274 986
Fragments arranged by size:
2154 986 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 5171
BseYI C'CCAG_C
Cuts at: 2596 3354 4019 4427 2596
Size: 758 665 408 3340
Fragments arranged by size:
3340 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2713 2759 3989 104
Size: 2609 46 1230 1286
Fragments arranged by size:
2609 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1795 1895 2185 2963 3153 4013 1795
Size: 100 290 778 190 860 2953
Fragments arranged by size:
2953 860 778 290 190 100
BsmI GAATG_Cn'
Cuts at: 1024 1354 1597 1024
Size: 330 243 4598
Fragments arranged by size:
4598 330 243
Bsp1286I G_dGCh'C
Cuts at: 1795 1895 2185 2796 2889 2963 3153 3215 4013
Size: 100 290 611 93 74 190 62 798
Cuts at: 4013 1795
Size: 2953
Fragments arranged by size:
2953 798 611 290 190 100 93 74
62
BspHI T'CATG_A
Cuts at: 969 1557 3603 969
Size: 588 2046 2537
Fragments arranged by size:
2537 2046 588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2221 2435 2737 3118 2221
Size: 214 302 381 4274
Fragments arranged by size:
4274 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3463 3517 5001 5166 50
Size: 3413 54 1484 165 55
Fragments arranged by size:
3413 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 450 1046 1550 1602 3083 450
Size: 596 504 52 1481 2538
Fragments arranged by size:
2538 1481 596 504 52
BsrFI r'CCGG_y
Cuts at: 3169 3350 3169
Size: 181 4990
BsrGI T'GTAC_A
Cuts at: 1302 1302
Size: 5171
BssSI C'ACGA_G
Cuts at: 3442 4150 3442
Size: 708 4463
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 2548 1129
Size: 1419 3752
BstBI TT'CG_AA
Cuts at: 3532 3532
Size: 5171
BstYI r'GATC_y
Cuts at: 1 183 2196 2663 2686 3021 3267 3573 3585
Size: 182 2013 467 23 335 246 306 12
Cuts at: 3585 3671 4723 1
Size: 86 1052 449
Fragments arranged by size:
2013 1052 467 449 335 306 246 182
86 23 12
BtgI C'CryG_G
Cuts at: 304 304
Size: 5171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3121 3302 3465 3121
Size: 181 163 4827
Fragments arranged by size:
4827 181 163
BtsI GCAGTG_nn'
Cuts at: 1795 2414 1795
Size: 619 4552
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1584 1619 1584
Size: 35 5136
DraI TTT'AAA
Cuts at: 2170 2170
Size: 5171
DrdI GACnn_nn'nnGTC
Cuts at: 2877 4221 2877
Size: 1344 3827
EaeI y'GGCC_r
Cuts at: 2756 2930 3321 3348 4693 2756
Size: 174 391 27 1345 3234
Fragments arranged by size:
3234 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2756 2756
Size: 5171
EarI CTCTTCn'nnn_
Cuts at: 169 1139 3194 3404 169
Size: 970 2055 210 1936
Fragments arranged by size:
2055 1936 970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3379 4121 4267 4981 3379
Size: 742 146 714 3569
Fragments arranged by size:
3569 742 714 146
EcoICRI GAG'CTC
Cuts at: 2183 2183
Size: 5171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 755 759 923 927 1007 1347 1595 1599
Size: 303 4 164 4 80 340 248 4
Cuts at: 1599 1679 2996 3428 3781 4443 452
Size: 80 1317 432 353 662 1180
Fragments arranged by size:
1317 1180 662 432 353 340 303 248
164 80 80 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2310 2310
Size: 5171
EcoRI G'AATT_C
Cuts at: 725 893 1229 1481 2153 2175 725
Size: 168 336 252 672 22 3721
Fragments arranged by size:
3721 672 336 252 168 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1395 1647 3190 1395
Size: 252 1543 3376
Fragments arranged by size:
3376 1543 252
FspI TGC'GCA
Cuts at: 2952 2952
Size: 5171
HaeII r_GCGC'y
Cuts at: 2605 2853 4083 4854 2605
Size: 248 1230 771 2922
Fragments arranged by size:
2922 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 497 529 1446 1478 3013 3043 3045
Size: 32 297 32 917 32 1535 30 2
Cuts at: 3045 3075 168
Size: 30 2264
Fragments arranged by size:
2264 1535 917 297 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2210 2210
Size: 5171
HindIII A'AGCT_T
Cuts at: 854 1442 854
Size: 588 4583
KasI G'GCGC_C
Cuts at: 2601 2849 2601
Size: 248 4923
KpnI G_GTAC'C
Cuts at: 2191 2191
Size: 5171
MluI A'CGCG_T
Cuts at: 4604 4604
Size: 5171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3930 4114 3930
Size: 184 4987
MscI TGG'CCA
Cuts at: 2932 2932
Size: 5171
MslI CAynn'nnrTG
Cuts at: 569 865 1238 1322 1826 1910 1994 2078 2162
Size: 296 373 84 504 84 84 84 84
Cuts at: 2162 3160 3287 569
Size: 998 127 2453
Fragments arranged by size:
2453 998 504 373 296 127 84 84
84 84 84
MspA1I CmG'CkG
Cuts at: 2282 2596 2956 3740 3985 2282
Size: 314 360 784 245 3468
Fragments arranged by size:
3468 784 360 314 245
NaeI GCC'GGC
Cuts at: 3352 3352
Size: 5171
NarI GG'CG_CC
Cuts at: 2602 2850 2602
Size: 248 4923
NcoI C'CATG_G
Cuts at: 304 304
Size: 5171
NdeI CA'TA_TG
Cuts at: 627 1139 1307 1811 2063 627
Size: 512 168 504 252 3735
Fragments arranged by size:
3735 512 504 252 168
NgoMIV G'CCGG_C
Cuts at: 3350 3350
Size: 5171
NsiI A_TGCA'T
Cuts at: 921 1089 921
Size: 168 5003
NspI r_CATG'y
Cuts at: 923 1091 1225 2224 2724 4327 923
Size: 168 134 999 500 1603 1767
Fragments arranged by size:
1767 1603 999 500 168 134
PasI CC'CwG_GG
Cuts at: 120 120
Size: 5171
PciI A'CATG_T
Cuts at: 1221 4323 1221
Size: 3102 2069
PmeI GTTT'AAAC
Cuts at: 2170 2170
Size: 5171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 3617
Size: 32 5139
PsiI TTA'TAA
Cuts at: 806 1169 806
Size: 363 4808
PstI C_TGCA'G
Cuts at: 2218 2903 2218
Size: 685 4486
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5171
PvuII CAG'CTG
Cuts at: 2596 2956 2596
Size: 360 4811
RsrII CG'GwC_CG
Cuts at: 3366 3366
Size: 5171
SacI G_AGCT'C
Cuts at: 2185 2185
Size: 5171
SalI G'TCGA_C
Cuts at: 2208 2208
Size: 5171
SapI GCTCTTCn'nnn_
Cuts at: 1139 3194 3404 1139
Size: 2055 210 2906
Fragments arranged by size:
2906 2055 210
SbfI CC_TGCA'GG
Cuts at: 2218 2218
Size: 5171
ScaI AGT'ACT
Cuts at: 4688 4688
Size: 5171
SfcI C'TryA_G
Cuts at: 33 762 2013 2214 2899 3867 4058 33
Size: 729 1251 201 685 968 191 1146
Fragments arranged by size:
1251 1146 968 729 685 201 191
SfoI GGC'GCC
Cuts at: 2603 2851 2603
Size: 248 4923
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5171
SmaI CCC'GGG
Cuts at: 2193 2193
Size: 5171
SmlI C'TyrA_G
Cuts at: 2326 3241 3955 4217 2326
Size: 915 714 262 3280
Fragments arranged by size:
3280 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5171
SphI G_CATG'C
Cuts at: 2224 2724 2224
Size: 500 4671
StyI C'CwwG_G
Cuts at: 304 2305 2659 304
Size: 2001 354 2816
Fragments arranged by size:
2816 2001 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 297 2754 2887 3346 3555 297
Size: 2457 133 459 209 1913
Fragments arranged by size:
2457 1913 459 209 133
TatI w'GTAC_w
Cuts at: 1302 4686 1302
Size: 3384 1787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4688 4720 4688
Size: 32 5139
Tth111I GACn'n_nGTC
Cuts at: 114 2968 114
Size: 2854 2317
XbaI T'CTAG_A
Cuts at: 2202 2202
Size: 5171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2666 2666
Size: 5171
XmaI C'CCGG_G
Cuts at: 2191 2191
Size: 5171
XmnI GAAnn'nnTTC
Cuts at: 940 977 1378 1565 1864 2005 940
Size: 37 401 187 299 141 4106
Fragments arranged by size:
4106 401 299 187 141 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
PacI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI