Restriction map for
pF1KB8733
(Circular) (Six-base) MAPSORT of: pF1KB8733.seq Check: 341 from: 1 to: 4577
pF1KB8733 4577 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 723 723
Size: 4577
AccI GT'mk_AC
Cuts at: 1191 1615 1191
Size: 424 4153
Acc65I G'GTAC_C
Cuts at: 1593 1593
Size: 4577
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 926 954 2402 2834 3581 3849 235
Size: 691 28 1448 432 747 268 963
Fragments arranged by size:
1448 963 747 691 432 268 28
AflII C'TTAA_G
Cuts at: 883 883
Size: 4577
AflIII A'CryG_T
Cuts at: 1929 3033 4010 1929
Size: 1104 977 2496
Fragments arranged by size:
2496 1104 977
AgeI A'CCGG_T
Cuts at: 2965 2965
Size: 4577
AleI CACnn'nnGTG
Cuts at: 526 526
Size: 4577
AlwNI CAG_nnn'CTG
Cuts at: 600 1882 3449 600
Size: 1282 1567 1728
Fragments arranged by size:
1728 1567 1282
ApaI G_GGCC'C
Cuts at: 519 519
Size: 4577
ApaLI G'TGCA_C
Cuts at: 3347 3347
Size: 4577
ApoI r'AATT_y
Cuts at: 1581 3930 4186 1581
Size: 2349 256 1972
Fragments arranged by size:
2349 1972 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 1597 1597
Size: 4577
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 929 962 929
Size: 33 4544
BamHI G'GATC_C
Cuts at: 1602 1602
Size: 4577
BanI G'GyrC_C
Cuts at: 1593 2007 2255 2290 1593
Size: 414 248 35 3880
Fragments arranged by size:
3880 414 248 35
BanII G_rGCy'C
Cuts at: 519 587 653 1591 2621 519
Size: 68 66 938 1030 2475
Fragments arranged by size:
2475 1030 938 68 66
BbeI G_GCGC'C
Cuts at: 2011 2259 2011
Size: 248 4329
BbsI GAAGACnn'nnnn_
Cuts at: 931 931
Size: 4577
BbvCI CC'TCA_GC
Cuts at: 448 448
Size: 4577
BciVI GTATCCnnnnn_n'
Cuts at: 957 2475 3236 4577 957
Size: 1518 761 1341 957
Fragments arranged by size:
1518 1341 957 761
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BglI GCCn_nnn'nGGC
Cuts at: 835 1268 835
Size: 433 4144
BglII A'GATC_T
Cuts at: 683 2092 4129 683
Size: 1409 2037 1131
Fragments arranged by size:
2037 1409 1131
BlpI GC'TnA_GC
Cuts at: 1689 1689
Size: 4577
Bme1580I G_kGCm'C
Cuts at: 245 519 2202 2295 3351 245
Size: 274 1683 93 1056 1471
Fragments arranged by size:
1683 1471 1056 274 93
BmrI ACTGGGnnnn_n'
Cuts at: 1209 1239 1789 1870 2205 4349 1209
Size: 30 550 81 335 2144 1437
Fragments arranged by size:
2144 1437 550 335 81 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 373 601 226
Size: 147 228 4202
Fragments arranged by size:
4202 228 147
Bpu10I CC'TnA_GC
Cuts at: 448 448
Size: 4577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 708 1753 2632 3124 3422 637
Size: 71 1045 879 492 298 1792
Fragments arranged by size:
1792 1045 879 492 298 71
BsaI GGTCTCn'nnnn_
Cuts at: 4281 4281
Size: 4577
BsaAI yAC'GTr
Cuts at: 1930 1930
Size: 4577
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 2008 2256 2958 4454 2008
Size: 248 702 1496 2131
Fragments arranged by size:
2131 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1766 2287 2965 3239 3386 3797 1766
Size: 521 678 274 147 411 2546
Fragments arranged by size:
2546 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 841 871 841
Size: 30 4547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 646
Size: 4577
BseYI C'CCAG_C
Cuts at: 133 2002 2760 3337 3833 133
Size: 1869 758 577 496 877
Fragments arranged by size:
1869 877 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1520
Size: 4577
BsiEI CG_ry'CG
Cuts at: 104 2119 2165 3373 104
Size: 2015 46 1208 1308
Fragments arranged by size:
2015 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 653 1591 2369 2559 3351 653
Size: 938 778 190 792 1879
Fragments arranged by size:
1879 938 792 778 190
BsmI GAATG_Cn'
Cuts at: 1536 1536
Size: 4577
Bsp1286I G_dGCh'C
Cuts at: 245 519 587 653 1591 2202 2295 2369 2559
Size: 274 68 66 938 611 93 74 190
Cuts at: 2559 2621 3351 245
Size: 62 730 1471
Fragments arranged by size:
1471 938 730 611 274 190 93 74
68 66 62
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 723 1627 1841 2143 2524 723
Size: 904 214 302 381 2776
Fragments arranged by size:
2776 904 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1448 2869 2923 4407 4572 50
Size: 1398 1421 54 1484 165 55
Fragments arranged by size:
1484 1421 1398 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 1414 2575 2756 2965 1414
Size: 1161 181 209 3026
Fragments arranged by size:
3026 1161 209 181
BssSI C'ACGA_G
Cuts at: 2848 3206 2848
Size: 358 4219
BstAPI GCAn_nnn'nTGC
Cuts at: 260 738 1954 260
Size: 478 1216 2883
Fragments arranged by size:
2883 1216 478
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstXI CCAn_nnnn'nTGG
Cuts at: 957 1565 957
Size: 608 3969
BstYI r'GATC_y
Cuts at: 1 407 683 1119 1602 2069 2092 2427 2673
Size: 406 276 436 483 467 23 335 246
Cuts at: 2673 3685 3771 3783 4129 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 483 467 449 436 406 346 335
276 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1000 1185 1000
Size: 185 4392
BtgI C'CryG_G
Cuts at: 107 929 107
Size: 822 3755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 1241 1820 1241
Size: 579 3998
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 2283 3141 2283
Size: 858 3719
EaeI y'GGCC_r
Cuts at: 2162 2336 2727 2754 4099 2162
Size: 174 391 27 1345 2640
Fragments arranged by size:
2640 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2162 2162
Size: 4577
EarI CTCTTCn'nnn_
Cuts at: 695 719 752 1504 2600 2810 695
Size: 24 33 752 1096 210 2462
Fragments arranged by size:
2462 1096 752 210 33 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2785 3095 3241 4387 2785
Size: 310 146 1146 2975
Fragments arranged by size:
2975 1146 310 146
EcoICRI GAG'CTC
Cuts at: 651 1589 651
Size: 938 3639
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 235 373 601 926 954 2402 2834 3581
Size: 9 138 228 325 28 1448 432 747
Cuts at: 3581 3849 226
Size: 268 954
Fragments arranged by size:
1448 954 747 432 325 268 228 138
28 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1075 1075
Size: 4577
EcoO109I rG'GnC_Cy
Cuts at: 137 282 444 822 1553 1716 137
Size: 145 162 378 731 163 2998
Fragments arranged by size:
2998 731 378 163 162 145
EcoRI G'AATT_C
Cuts at: 1581 1581
Size: 4577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4577
FspI TGC'GCA
Cuts at: 2358 2358
Size: 4577
HaeII r_GCGC'y
Cuts at: 819 2011 2259 3281 4260 819
Size: 1192 248 1022 979 1136
Fragments arranged by size:
1192 1136 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 668 700 1041 1073 2419 2449 2451 2481 668
Size: 32 341 32 1346 30 2 30 2764
Fragments arranged by size:
2764 1346 341 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1192 1277 1616 1192
Size: 85 339 4153
Fragments arranged by size:
4153 339 85
HindIII A'AGCT_T
Cuts at: 257 257
Size: 4577
KasI G'GCGC_C
Cuts at: 2007 2255 2007
Size: 248 4329
KpnI G_GTAC'C
Cuts at: 1597 1597
Size: 4577
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 366 3248 3432 366
Size: 2882 184 1511
Fragments arranged by size:
2882 1511 184
MscI TGG'CCA
Cuts at: 2338 2338
Size: 4577
MslI CAynn'nnrTG
Cuts at: 526 934 1372 2566 2693 526
Size: 408 438 1194 127 2410
Fragments arranged by size:
2410 1194 438 408 127
MspA1I CmG'CkG
Cuts at: 157 272 1688 2002 2362 3375 3620 157
Size: 115 1416 314 360 1013 245 1114
Fragments arranged by size:
1416 1114 1013 360 314 245 115
NaeI GCC'GGC
Cuts at: 1416 2758 1416
Size: 1342 3235
NarI GG'CG_CC
Cuts at: 2008 2256 2008
Size: 248 4329
NcoI C'CATG_G
Cuts at: 107 929 107
Size: 822 3755
NgoMIV G'CCGG_C
Cuts at: 1414 2756 1414
Size: 1342 3235
NspI r_CATG'y
Cuts at: 1630 2130 3037 1630
Size: 500 907 3170
Fragments arranged by size:
3170 907 500
PciI A'CATG_T
Cuts at: 3033 3033
Size: 4577
PfoI T'CCnGG_A
Cuts at: 410 1115 410
Size: 705 3872
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4545
PpuMI rG'GwC_Cy
Cuts at: 282 822 282
Size: 540 4037
PspOMI G'GGCC_C
Cuts at: 515 515
Size: 4577
PstI C_TGCA'G
Cuts at: 147 572 605 779 1624 2309 147
Size: 425 33 174 845 685 2415
Fragments arranged by size:
2415 845 685 425 174 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 157 2002 2362 157
Size: 1845 360 2372
Fragments arranged by size:
2372 1845 360
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 653 1591 653
Size: 938 3639
SalI G'TCGA_C
Cuts at: 1190 1614 1190
Size: 424 4153
SapI GCTCTTCn'nnn_
Cuts at: 695 719 2600 2810 695
Size: 24 1881 210 2462
Fragments arranged by size:
2462 1881 210 24
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 991 4094 991
Size: 3103 1474
SfcI C'TryA_G
Cuts at: 33 143 152 568 601 775 1620 2305 3298
Size: 110 9 416 33 174 845 685 993
Cuts at: 3298 3489 33
Size: 191 1121
Fragments arranged by size:
1121 993 845 685 416 191 174 110
33 9
SfoI GGC'GCC
Cuts at: 2009 2257 2009
Size: 248 4329
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 1599 1599
Size: 4577
SmlI C'TyrA_G
Cuts at: 652 687 883 1732 2647 3139 3401 652
Size: 35 196 849 915 492 262 1828
Fragments arranged by size:
1828 915 849 492 262 196 35
SpeI A'CTAG_T
Cuts at: 67 660 67
Size: 593 3984
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
StyI C'CwwG_G
Cuts at: 107 804 929 1135 1711 2065 107
Size: 697 125 206 576 354 2619
Fragments arranged by size:
2619 697 576 354 206 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 1228 2160 2293 2752 2961 523
Size: 705 932 133 459 209 2139
Fragments arranged by size:
2139 932 705 459 209 133
TatI w'GTAC_w
Cuts at: 554 989 4092 554
Size: 435 3103 1039
Fragments arranged by size:
3103 1039 435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 841 873 4094 4126 841
Size: 32 3221 32 1292
Fragments arranged by size:
3221 1292 32 32
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2072 2072
Size: 4577
XmaI C'CCGG_G
Cuts at: 1597 1597
Size: 4577
XmnI GAAnn'nnTTC
Cuts at: 704 704
Size: 4577
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BcgI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI