Restriction map for
pF1KB8735
(Circular) (Six-base) MAPSORT of: pF1KB8735.seq Check: 7567 from: 1 to: 3935
pF1KB8735 3935 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 761 761
Size: 3935
AccI GT'mk_AC
Cuts at: 973 973
Size: 3935
Acc65I G'GTAC_C
Cuts at: 951 951
Size: 3935
AclI AA'CG_TT
Cuts at: 3775 3775
Size: 3935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1760 2192 2939 3207 1760
Size: 432 747 268 2488
Fragments arranged by size:
2488 747 432 268
AflIII A'CryG_T
Cuts at: 131 545 1287 2391 3368 131
Size: 414 742 1104 977 698
Fragments arranged by size:
1104 977 742 698 414
AgeI A'CCGG_T
Cuts at: 2323 2323
Size: 3935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 223 223
Size: 3935
AlwNI CAG_nnn'CTG
Cuts at: 258 1240 2807 258
Size: 982 1567 1386
Fragments arranged by size:
1567 1386 982
ApaI G_GGCC'C
Cuts at: 425 425
Size: 3935
ApaLI G'TGCA_C
Cuts at: 394 400 2705 394
Size: 6 2305 1624
Fragments arranged by size:
2305 1624 6
ApoI r'AATT_y
Cuts at: 939 3288 3544 939
Size: 2349 256 1330
Fragments arranged by size:
2349 1330 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3935
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
AvaI C'yCGr_G
Cuts at: 289 315 955 289
Size: 26 640 3269
Fragments arranged by size:
3269 640 26
BamHI G'GATC_C
Cuts at: 960 960
Size: 3935
BanI G'GyrC_C
Cuts at: 124 227 324 363 501 951 1365 1613 1648
Size: 103 97 39 138 450 414 248 35
Cuts at: 1648 124
Size: 2411
Fragments arranged by size:
2411 450 414 248 138 103 97 39
35
BanII G_rGCy'C
Cuts at: 281 313 425 851 866 949 1979 281
Size: 32 112 426 15 83 1030 2237
Fragments arranged by size:
2237 1030 426 112 83 32 15
BbeI G_GCGC'C
Cuts at: 231 328 367 1369 1617 231
Size: 97 39 1002 248 2549
Fragments arranged by size:
2549 1002 248 97 39
BbsI GAAGACnn'nnnn_
Cuts at: 816 816
Size: 3935
BciVI GTATCCnnnnn_n'
Cuts at: 1833 2594 3935 1833
Size: 761 1341 1833
Fragments arranged by size:
1833 1341 761
BclI T'GATC_A
Cuts at: 1455 1455
Size: 3935
BglI GCCn_nnn'nGGC
Cuts at: 317 510 317
Size: 193 3742
BglII A'GATC_T
Cuts at: 1450 3487 1450
Size: 2037 1898
BlpI GC'TnA_GC
Cuts at: 850 1047 850
Size: 197 3738
Bme1580I G_kGCm'C
Cuts at: 127 398 404 425 1560 1653 2709 127
Size: 271 6 21 1135 93 1056 1353
Fragments arranged by size:
1353 1135 1056 271 93 21 6
BmrI ACTGGGnnnn_n'
Cuts at: 1147 1228 1563 3707 1147
Size: 81 335 2144 1375
Fragments arranged by size:
2144 1375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 3935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 379 537 889 295
Size: 84 158 352 3341
Fragments arranged by size:
3341 352 158 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1990 2482 2780 1111
Size: 879 492 298 2266
Fragments arranged by size:
2266 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 178 900 3639 178
Size: 722 2739 474
Fragments arranged by size:
2739 722 474
BsaAI yAC'GTr
Cuts at: 143 393 399 1288 143
Size: 250 6 889 2790
Fragments arranged by size:
2790 889 250 6
BsaBI GATnn'nnATC
Cuts at: 6 1449 3486 6
Size: 1443 2037 455
Fragments arranged by size:
2037 1443 455
BsaHI Gr'CG_yC
Cuts at: 228 325 364 885 1366 1614 2316 3812 228
Size: 97 39 521 481 248 702 1496 351
Fragments arranged by size:
1496 702 521 481 351 248 97 39
BsaWI w'CCGG_w
Cuts at: 1124 1645 2323 2597 2744 3155 1124
Size: 521 678 274 147 411 1904
Fragments arranged by size:
1904 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 250
Size: 3935
BseYI C'CCAG_C
Cuts at: 703 757 786 829 1360 2118 2695 3191 703
Size: 54 29 43 531 758 577 496 1447
Fragments arranged by size:
1447 758 577 531 496 54 43 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 733 397
Size: 336 3599
BsiEI CG_ry'CG
Cuts at: 104 810 1477 1523 2731 104
Size: 706 667 46 1208 1308
Fragments arranged by size:
1308 1208 706 667 46
BsiHKAI G_wGCw'C
Cuts at: 398 404 617 851 875 949 1727 1917 2709
Size: 6 213 234 24 74 778 190 792
Cuts at: 2709 398
Size: 1624
Fragments arranged by size:
1624 792 778 234 213 190 74 24
6
Bsp1286I G_dGCh'C
Cuts at: 127 281 313 398 404 425 617 851 866
Size: 154 32 85 6 21 192 234 15
Cuts at: 866 875 949 1560 1653 1727 1917 1979 2709
Size: 9 74 611 93 74 190 62 730
Cuts at: 2709 127
Size: 1353
Fragments arranged by size:
1353 730 611 234 192 190 154 93
85 74 74 62 32 21 15 9
6
BspEI T'CCGG_A
Cuts at: 3155 3155
Size: 3935
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 761 985 1199 1501 1882 266
Size: 495 224 214 302 381 2319
Fragments arranged by size:
2319 495 381 302 224 214
BsrBI CCG'CTC
Cuts at: 50 717 2227 2281 3765 3930 50
Size: 667 1510 54 1484 165 55
Fragments arranged by size:
1510 1484 667 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1847 1847
Size: 3935
BsrFI r'CCGG_y
Cuts at: 151 366 891 1933 2114 2323 151
Size: 215 525 1042 181 209 1763
Fragments arranged by size:
1763 1042 525 215 209 181
BssHII G'CGCG_C
Cuts at: 261 261
Size: 3935
BssSI C'ACGA_G
Cuts at: 2206 2564 2206
Size: 358 3577
BstAPI GCAn_nnn'nTGC
Cuts at: 258 408 1312 258
Size: 150 904 2881
Fragments arranged by size:
2881 904 150
BstBI TT'CG_AA
Cuts at: 2296 2296
Size: 3935
BstXI CCAn_nnnn'nTGG
Cuts at: 515 515
Size: 3935
BstYI r'GATC_y
Cuts at: 1 960 1427 1450 1785 2031 3043 3129 3141
Size: 959 467 23 335 246 1012 86 12
Cuts at: 3141 3487 1
Size: 346 449
Fragments arranged by size:
1012 959 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 417 508 810 107
Size: 310 91 302 3232
Fragments arranged by size:
3232 310 302 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1885 2066 2229 1885
Size: 181 163 3591
Fragments arranged by size:
3591 181 163
BtsI GCAGTG_nn'
Cuts at: 1178 1178
Size: 3935
DraI TTT'AAA
Cuts at: 934 934
Size: 3935
DrdI GACnn_nn'nnGTC
Cuts at: 1641 2499 1641
Size: 858 3077
EaeI y'GGCC_r
Cuts at: 302 813 1520 1694 2085 2112 3457 302
Size: 511 707 174 391 27 1345 780
Fragments arranged by size:
1345 780 707 511 391 174 27
EagI C'GGCC_G
Cuts at: 1520 1520
Size: 3935
EarI CTCTTCn'nnn_
Cuts at: 563 724 1958 2168 563
Size: 161 1234 210 2330
Fragments arranged by size:
2330 1234 210 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2143 2453 2599 3745 2143
Size: 310 146 1146 2333
Fragments arranged by size:
2333 1146 310 146
EcoICRI GAG'CTC
Cuts at: 849 947 849
Size: 98 3837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 379 537 889 1760 2192 2939 3207 295
Size: 84 158 352 871 432 747 268 1023
Fragments arranged by size:
1023 871 747 432 352 268 158 84
EcoO109I rG'GnC_Cy
Cuts at: 1074 1074
Size: 3935
EcoRI G'AATT_C
Cuts at: 939 939
Size: 3935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 735 1954 735
Size: 1219 2716
FspI TGC'GCA
Cuts at: 1716 1716
Size: 3935
HaeII r_GCGC'y
Cuts at: 231 328 367 748 764 1369 1617 2639 3618
Size: 97 39 381 16 605 248 1022 979
Cuts at: 3618 231
Size: 548
Fragments arranged by size:
1022 979 605 548 381 248 97 39
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1777 1807 1809 1839 1777
Size: 30 2 30 3873
Fragments arranged by size:
3873 30 30 2
HincII GTy'rAC
Cuts at: 974 974
Size: 3935
KasI G'GCGC_C
Cuts at: 227 324 363 1365 1613 227
Size: 97 39 1002 248 2549
Fragments arranged by size:
2549 1002 248 97 39
KpnI G_GTAC'C
Cuts at: 955 955
Size: 3935
MluI A'CGCG_T
Cuts at: 3368 3368
Size: 3935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2790 2606
Size: 184 3751
MscI TGG'CCA
Cuts at: 1696 1696
Size: 3935
MslI CAynn'nnrTG
Cuts at: 112 1924 2051 112
Size: 1812 127 1996
Fragments arranged by size:
1996 1812 127
MspA1I CmG'CkG
Cuts at: 408 833 1046 1360 1720 2733 2978 408
Size: 425 213 314 360 1013 245 1365
Fragments arranged by size:
1365 1013 425 360 314 245 213
NaeI GCC'GGC
Cuts at: 153 368 2116 153
Size: 215 1748 1972
Fragments arranged by size:
1972 1748 215
NarI GG'CG_CC
Cuts at: 228 325 364 1366 1614 228
Size: 97 39 1002 248 2549
Fragments arranged by size:
2549 1002 248 97 39
NcoI C'CATG_G
Cuts at: 107 107
Size: 3935
NgoMIV G'CCGG_C
Cuts at: 151 366 2114 151
Size: 215 1748 1972
Fragments arranged by size:
1972 1748 215
NspI r_CATG'y
Cuts at: 549 988 1488 2395 549
Size: 439 500 907 2089
Fragments arranged by size:
2089 907 500 439
PasI CC'CwG_GG
Cuts at: 120 120
Size: 3935
PciI A'CATG_T
Cuts at: 545 2391 545
Size: 1846 2089
PfoI T'CCnGG_A
Cuts at: 722 722
Size: 3935
PmeI GTTT'AAAC
Cuts at: 934 934
Size: 3935
PmlI CAC'GTG
Cuts at: 143 393 399 143
Size: 250 6 3679
Fragments arranged by size:
3679 250 6
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3903
PspOMI G'GGCC_C
Cuts at: 421 421
Size: 3935
PspXI vC'TCGA_Gb
Cuts at: 289 289
Size: 3935
PstI C_TGCA'G
Cuts at: 202 803 982 1667 202
Size: 601 179 685 2470
Fragments arranged by size:
2470 685 601 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3935
PvuII CAG'CTG
Cuts at: 833 1360 1720 833
Size: 527 360 3048
Fragments arranged by size:
3048 527 360
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 3935
SacI G_AGCT'C
Cuts at: 851 949 851
Size: 98 3837
SalI G'TCGA_C
Cuts at: 972 972
Size: 3935
SapI GCTCTTCn'nnn_
Cuts at: 724 1958 2168 724
Size: 1234 210 2491
Fragments arranged by size:
2491 1234 210
SbfI CC_TGCA'GG
Cuts at: 803 982 803
Size: 179 3756
ScaI AGT'ACT
Cuts at: 3452 3452
Size: 3935
SfcI C'TryA_G
Cuts at: 33 198 603 799 920 978 1663 2656 2847
Size: 165 405 196 121 58 685 993 191
Cuts at: 2847 33
Size: 1121
Fragments arranged by size:
1121 993 685 405 196 191 165 121
58
SfoI GGC'GCC
Cuts at: 229 326 365 1367 1615 229
Size: 97 39 1002 248 2549
Fragments arranged by size:
2549 1002 248 97 39
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
SmaI CCC'GGG
Cuts at: 317 957 317
Size: 640 3295
SmlI C'TyrA_G
Cuts at: 289 1090 2005 2497 2759 289
Size: 801 915 492 262 1465
Fragments arranged by size:
1465 915 801 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3935
SphI G_CATG'C
Cuts at: 988 1488 988
Size: 500 3435
StyI C'CwwG_G
Cuts at: 107 1069 1423 107
Size: 962 354 2619
Fragments arranged by size:
2619 962 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 464 853 1518 1651 2110 2319 464
Size: 389 665 133 459 209 2080
Fragments arranged by size:
2080 665 459 389 209 133
TatI w'GTAC_w
Cuts at: 3450 3450
Size: 3935
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 3903
Tth111I GACn'n_nGTC
Cuts at: 1732 1732
Size: 3935
XbaI T'CTAG_A
Cuts at: 966 966
Size: 3935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1430 1430
Size: 3935
XhoI C'TCGA_G
Cuts at: 289 289
Size: 3935
XmaI C'CCGG_G
Cuts at: 315 955 315
Size: 640 3295
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PpuMI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI