Restriction map for
pF1KB8736
(Circular) (Six-base) MAPSORT of: pF1KB8736.seq Check: 2120 from: 1 to: 4313
pF1KB8736 4313 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1351 1351
Size: 4313
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 897 1065 1149 2138 2570 2923 3585 192
Size: 705 168 84 989 432 353 662 920
Fragments arranged by size:
989 920 705 662 432 353 168 84
AflIII A'CryG_T
Cuts at: 1665 3465 3746 1665
Size: 1800 281 2232
Fragments arranged by size:
2232 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 815 1618 3056 815
Size: 803 1438 2072
Fragments arranged by size:
2072 1438 803
ApaLI G'TGCA_C
Cuts at: 3151 3151
Size: 4313
ApoI r'AATT_y
Cuts at: 133 371 658 1317 3666 3922 133
Size: 238 287 659 2349 256 524
Fragments arranged by size:
2349 659 524 287 256 238
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 1333 1333
Size: 4313
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 767 1329 1743 1991 2026 767
Size: 562 414 248 35 3054
Fragments arranged by size:
3054 562 414 248 35
BanII G_rGCy'C
Cuts at: 284 318 1093 1327 2357 284
Size: 34 775 234 1030 2240
Fragments arranged by size:
2240 1030 775 234 34
BbeI G_GCGC'C
Cuts at: 771 1747 1995 771
Size: 976 248 3089
Fragments arranged by size:
3089 976 248
BciVI GTATCCnnnnn_n'
Cuts at: 249 2211 3267 4313 249
Size: 1962 1056 1046 249
Fragments arranged by size:
1962 1056 1046 249
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1425 1425
Size: 4313
Bme1580I G_kGCm'C
Cuts at: 1938 2031 3155 1938
Size: 93 1124 3096
Fragments arranged by size:
3096 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1606 1941 4085 1525
Size: 81 335 2144 1753
Fragments arranged by size:
2144 1753 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 944 1196 2680 944
Size: 252 1484 2577
Fragments arranged by size:
2577 1484 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 809 893 977 1061 1145 1229 220
Size: 589 84 84 84 84 84 3304
Fragments arranged by size:
3304 589 84 84 84 84 84
Bpu10I CC'TnA_GC
Cuts at: 547 585 547
Size: 38 4275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2368 3082 3380 1489
Size: 879 714 298 2422
Fragments arranged by size:
2422 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 768 1744 1992 2694 4190 768
Size: 976 248 702 1496 891
Fragments arranged by size:
1496 976 891 702 248
BsaWI w'CCGG_w
Cuts at: 1502 2023 2701 3112 3259 3533 1502
Size: 521 678 411 147 274 2282
Fragments arranged by size:
2282 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1738 2496 3161 3569 1738
Size: 758 665 408 2482
Fragments arranged by size:
2482 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 922
Size: 4313
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3131 104
Size: 1751 46 1230 1286
Fragments arranged by size:
1751 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1093 1327 2105 2295 3155 1093
Size: 234 778 190 860 2251
Fragments arranged by size:
2251 860 778 234 190
BsmI GAATG_Cn'
Cuts at: 620 811 895 1063 620
Size: 191 84 168 3870
Fragments arranged by size:
3870 191 168 84
BsmBI CGTCTCn'nnnn_
Cuts at: 932 932
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 284 318 1093 1327 1938 2031 2105 2295 2357
Size: 34 775 234 611 93 74 190 62
Cuts at: 2357 3155 284
Size: 798 1442
Fragments arranged by size:
1442 798 775 611 234 190 93 74
62 34
BspHI T'CATG_A
Cuts at: 2745 2745
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2605 2659 4143 4308 50
Size: 2555 54 1484 165 55
Fragments arranged by size:
2555 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2225 2225
Size: 4313
BsrFI r'CCGG_y
Cuts at: 2311 2492 2311
Size: 181 4132
BssSI C'ACGA_G
Cuts at: 2584 3292 2584
Size: 708 3605
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstYI r'GATC_y
Cuts at: 1 637 1338 1805 1828 2163 2409 2715 2727
Size: 636 701 467 23 335 246 306 12
Cuts at: 2727 2813 3865 1
Size: 86 1052 449
Fragments arranged by size:
1052 701 636 467 449 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 792 2263 2444 2607 792
Size: 1471 181 163 2498
Fragments arranged by size:
2498 1471 181 163
BtsI GCAGTG_nn'
Cuts at: 816 900 1556 816
Size: 84 656 3573
Fragments arranged by size:
3573 656 84
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 3363 2019
Size: 1344 2969
EaeI y'GGCC_r
Cuts at: 218 468 1898 2072 2463 2490 3835 218
Size: 250 1430 174 391 27 1345 696
Fragments arranged by size:
1430 1345 696 391 250 174 27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 325 816 2336 2546 325
Size: 491 1520 210 2092
Fragments arranged by size:
2092 1520 491 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2521 3263 3409 4123 2521
Size: 742 146 714 2711
Fragments arranged by size:
2711 742 714 146
EcoICRI GAG'CTC
Cuts at: 1091 1325 1091
Size: 234 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 220 809 893 897 977 1061 1065 1145
Size: 28 589 84 4 80 84 4 80
Cuts at: 1145 1149 1229 2138 2570 2923 3585 192
Size: 4 80 909 432 353 662 920
Fragments arranged by size:
920 909 662 589 432 353 84 84
80 80 80 28 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 177 1244 1452 177
Size: 1067 208 3038
Fragments arranged by size:
3038 1067 208
EcoRI G'AATT_C
Cuts at: 133 658 1317 133
Size: 525 659 3129
Fragments arranged by size:
3129 659 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 771 1747 1995 3225 3996 771
Size: 976 248 1230 771 1088
Fragments arranged by size:
1230 1088 976 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2155 2185 2187 2217 2155
Size: 30 2 30 4251
Fragments arranged by size:
4251 30 30 2
HincII GTy'rAC
Cuts at: 521 609 1352 521
Size: 88 743 3482
Fragments arranged by size:
3482 743 88
HindIII A'AGCT_T
Cuts at: 582 582
Size: 4313
HpaI GTT'AAC
Cuts at: 609 609
Size: 4313
KasI G'GCGC_C
Cuts at: 767 1743 1991 767
Size: 976 248 3089
Fragments arranged by size:
3089 976 248
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 245 3072 3256 245
Size: 2827 184 1302
Fragments arranged by size:
2827 1302 184
MscI TGG'CCA
Cuts at: 220 470 2074 220
Size: 250 1604 2459
Fragments arranged by size:
2459 1604 250
MslI CAynn'nnrTG
Cuts at: 625 2302 2429 625
Size: 1677 127 2509
Fragments arranged by size:
2509 1677 127
MspA1I CmG'CkG
Cuts at: 159 1424 1738 2098 2882 3127 159
Size: 1265 314 360 784 245 1345
Fragments arranged by size:
1345 1265 784 360 314 245
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 4313
NarI GG'CG_CC
Cuts at: 768 1744 1992 768
Size: 976 248 3089
Fragments arranged by size:
3089 976 248
NdeI CA'TA_TG
Cuts at: 249 249
Size: 4313
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 4313
NspI r_CATG'y
Cuts at: 1366 1866 3469 1366
Size: 500 1603 2210
Fragments arranged by size:
2210 1603 500
PciI A'CATG_T
Cuts at: 3465 3465
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 177 177
Size: 4313
PsiI TTA'TAA
Cuts at: 560 644 560
Size: 84 4229
PstI C_TGCA'G
Cuts at: 434 1360 2045 434
Size: 926 685 2702
Fragments arranged by size:
2702 926 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 159 1738 2098 159
Size: 1579 360 2374
Fragments arranged by size:
2374 1579 360
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1093 1327 1093
Size: 234 4079
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 816 2336 2546 816
Size: 1520 210 2583
Fragments arranged by size:
2583 1520 210
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 183 430 942 1356 2041 3009 3200 33
Size: 150 247 512 414 685 968 191 1146
Fragments arranged by size:
1146 968 685 512 414 247 191 150
SfoI GGC'GCC
Cuts at: 769 1745 1993 769
Size: 976 248 3089
Fragments arranged by size:
3089 976 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 1468 2383 3097 3359 1468
Size: 915 714 262 2422
Fragments arranged by size:
2422 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StyI C'CwwG_G
Cuts at: 347 1447 1801 347
Size: 1100 354 2859
Fragments arranged by size:
2859 1100 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 716 1896 2029 2488 2697 716
Size: 1180 133 459 209 2332
Fragments arranged by size:
2332 1180 459 209 133
TatI w'GTAC_w
Cuts at: 3828 3828
Size: 4313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 994 1026 3830 3862 994
Size: 32 2804 32 1445
Fragments arranged by size:
2804 1445 32 32
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmgBI BmtI BsaXI BseRI BsiWI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI