Restriction map for
pF1KB8740
(Circular) (Six-base) MAPSORT of: pF1KB8740.seq Check: 247 from: 1 to: 3662
pF1KB8740 3662 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 566 566
Size: 3662
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1487 1919 2666 2934 1487
Size: 432 747 268 2215
Fragments arranged by size:
2215 747 432 268
AflIII A'CryG_T
Cuts at: 365 1014 2118 3095 365
Size: 649 1104 977 932
Fragments arranged by size:
1104 977 932 649
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 529 530 561 562 529
Size: 1 31 1 3629
Fragments arranged by size:
3629 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 467 967 2534 467
Size: 500 1567 1595
Fragments arranged by size:
1595 1567 500
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 666 3015 3271 666
Size: 2349 256 1057
Fragments arranged by size:
2349 1057 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 244 324 359 682 244
Size: 80 35 323 3224
Fragments arranged by size:
3224 323 80 35
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 175 207 678 1092 1340 1375 175
Size: 32 471 414 248 35 2462
Fragments arranged by size:
2462 471 414 248 35 32
BanII G_rGCy'C
Cuts at: 676 1706 676
Size: 1030 2632
BbeI G_GCGC'C
Cuts at: 179 1096 1344 179
Size: 917 248 2497
Fragments arranged by size:
2497 917 248
BbsI GAAGACnn'nnnn_
Cuts at: 536 536
Size: 3662
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 330 364 390 424 330
Size: 34 26 34 3568
Fragments arranged by size:
3568 34 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglII A'GATC_T
Cuts at: 1177 3214 1177
Size: 2037 1625
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 212 233 1287 1380 2436 212
Size: 21 1054 93 1056 1438
Fragments arranged by size:
1438 1056 1054 93 21
BmgBI CAC'GTC
Cuts at: 228 228
Size: 3662
BmrI ACTGGGnnnn_n'
Cuts at: 307 874 955 1290 3434 307
Size: 567 81 335 2144 535
Fragments arranged by size:
2144 567 535 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 3662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 316
Size: 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 838 1717 2209 2507 373
Size: 465 879 492 298 1528
Fragments arranged by size:
1528 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 3662
BsaAI yAC'GTr
Cuts at: 381 1015 381
Size: 634 3028
BsaBI GATnn'nnATC
Cuts at: 6 115 1176 3213 6
Size: 109 1061 2037 455
Fragments arranged by size:
2037 1061 455 109
BsaHI Gr'CG_yC
Cuts at: 176 518 1093 1341 2043 3539 176
Size: 342 575 248 702 1496 299
Fragments arranged by size:
1496 702 575 342 299 248
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 469 313
Size: 156 3506
BseYI C'CCAG_C
Cuts at: 211 445 1087 1845 2422 2918 211
Size: 234 642 758 577 496 955
Fragments arranged by size:
955 758 642 577 496 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 553 409
Size: 144 3518
BsiEI CG_ry'CG
Cuts at: 104 1204 1250 2458 104
Size: 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1100 46
BsiHKAI G_wGCw'C
Cuts at: 581 676 1454 1644 2436 581
Size: 95 778 190 792 1807
Fragments arranged by size:
1807 792 778 190 95
Bsp1286I G_dGCh'C
Cuts at: 212 233 581 676 1287 1380 1454 1644 1706
Size: 21 348 95 611 93 74 190 62
Cuts at: 1706 2436 212
Size: 730 1438
Fragments arranged by size:
1438 730 611 348 190 95 93 74
62 21
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 2838 2838
Size: 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 566 712 926 1228 1609 566
Size: 146 214 302 381 2619
Fragments arranged by size:
2619 381 302 214 146
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1574 1574
Size: 3662
BsrFI r'CCGG_y
Cuts at: 441 520 1660 1841 2050 441
Size: 79 1140 181 209 2053
Fragments arranged by size:
2053 1140 209 181 79
BsrGI T'GTAC_A
Cuts at: 368 368
Size: 3662
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 576 1039 576
Size: 463 3199
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstYI r'GATC_y
Cuts at: 1 687 1154 1177 1512 1758 2770 2856 2868
Size: 686 467 23 335 246 1012 86 12
Cuts at: 2868 3214 1
Size: 346 449
Fragments arranged by size:
1012 686 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 1793 1956 1612
Size: 181 163 3318
Fragments arranged by size:
3318 181 163
BtsI GCAGTG_nn'
Cuts at: 288 468 905 288
Size: 180 437 3045
Fragments arranged by size:
3045 437 180
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DraIII CAC_nnn'GTG
Cuts at: 381 576 381
Size: 195 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 1247 1421 1812 1839 3184 1247
Size: 174 391 27 1345 1725
Fragments arranged by size:
1725 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1247 1247
Size: 3662
EarI CTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1870 2180 2326 3472 1870
Size: 310 146 1146 2060
Fragments arranged by size:
2060 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 674
Size: 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1487 1919 2666 2934 316
Size: 1171 432 747 268 1044
Fragments arranged by size:
1171 1044 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 420 801 420
Size: 381 3281
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3662
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 179 335 1096 1344 2366 3345 179
Size: 156 761 248 1022 979 496
Fragments arranged by size:
1022 979 761 496 248 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1504 1534 1536 1566 1504
Size: 30 2 30 3600
Fragments arranged by size:
3600 30 30 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 175 1092 1340 175
Size: 917 248 2497
Fragments arranged by size:
2497 917 248
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MfeI C'AATT_G
Cuts at: 645 645
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2517 2333
Size: 184 3478
MscI TGG'CCA
Cuts at: 1423 1423
Size: 3662
MslI CAynn'nnrTG
Cuts at: 487 1651 1778 487
Size: 1164 127 2371
Fragments arranged by size:
2371 1164 127
MspA1I CmG'CkG
Cuts at: 773 1087 1447 2460 2705 773
Size: 314 360 1013 245 1730
Fragments arranged by size:
1730 1013 360 314 245
NaeI GCC'GGC
Cuts at: 522 1843 522
Size: 1321 2341
NarI GG'CG_CC
Cuts at: 176 1093 1341 176
Size: 917 248 2497
Fragments arranged by size:
2497 917 248
NgoMIV G'CCGG_C
Cuts at: 520 1841 520
Size: 1321 2341
NspI r_CATG'y
Cuts at: 369 715 1215 2122 369
Size: 346 500 907 1909
Fragments arranged by size:
1909 907 500 346
PciI A'CATG_T
Cuts at: 365 2118 365
Size: 1753 1909
PflMI CCAn_nnn'nTGG
Cuts at: 445 445
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PpuMI rG'GwC_Cy
Cuts at: 420 420
Size: 3662
PstI C_TGCA'G
Cuts at: 709 1394 709
Size: 685 2977
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 1087 1447 1087
Size: 360 3302
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 676 676
Size: 3662
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SfcI C'TryA_G
Cuts at: 33 705 1390 2383 2574 33
Size: 672 685 993 191 1121
Fragments arranged by size:
1121 993 685 672 191
SfoI GGC'GCC
Cuts at: 177 1094 1342 177
Size: 917 248 2497
Fragments arranged by size:
2497 917 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SgrAI Cr'CCGG_yG
Cuts at: 520 520
Size: 3662
SmaI CCC'GGG
Cuts at: 246 684 246
Size: 438 3224
SmlI C'TyrA_G
Cuts at: 388 817 1732 2224 2486 388
Size: 429 915 492 262 1564
Fragments arranged by size:
1564 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
StyI C'CwwG_G
Cuts at: 476 796 1150 476
Size: 320 354 2988
Fragments arranged by size:
2988 354 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1245 1378 1837 2046 1245
Size: 133 459 209 2861
Fragments arranged by size:
2861 459 209 133
TatI w'GTAC_w
Cuts at: 368 3177 368
Size: 2809 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 3630
Tth111I GACn'n_nGTC
Cuts at: 1459 1459
Size: 3662
XbaI T'CTAG_A
Cuts at: 693 693
Size: 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 1157 483
Size: 674 2988
XmaI C'CCGG_G
Cuts at: 244 682 244
Size: 438 3224
XmnI GAAnn'nnTTC
Cuts at: 166 166
Size: 3662
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI ZraI