Restriction map for
pF1KB8741
(Circular) (Six-base) MAPSORT of: pF1KB8741.seq Check: 5910 from: 1 to: 3530
pF1KB8741 3530 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 568 568
Size: 3530
Acc65I G'GTAC_C
Cuts at: 546 546
Size: 3530
AclI AA'CG_TT
Cuts at: 3370 3370
Size: 3530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 1355 1787 2534 2802 455
Size: 900 432 747 268 1183
Fragments arranged by size:
1183 900 747 432 268
AflIII A'CryG_T
Cuts at: 882 1986 2963 882
Size: 1104 977 1449
Fragments arranged by size:
1449 1104 977
AgeI A'CCGG_T
Cuts at: 1918 1918
Size: 3530
AlwNI CAG_nnn'CTG
Cuts at: 268 835 2402 268
Size: 567 1567 1396
Fragments arranged by size:
1567 1396 567
ApaLI G'TGCA_C
Cuts at: 2300 2300
Size: 3530
ApoI r'AATT_y
Cuts at: 204 306 330 397 534 2883 3139 204
Size: 102 24 67 137 2349 256 595
Fragments arranged by size:
2349 595 256 137 102 67 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 3530
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3530
AvaI C'yCGr_G
Cuts at: 550 550
Size: 3530
BamHI G'GATC_C
Cuts at: 555 555
Size: 3530
BanI G'GyrC_C
Cuts at: 391 546 960 1208 1243 391
Size: 155 414 248 35 2678
Fragments arranged by size:
2678 414 248 155 35
BanII G_rGCy'C
Cuts at: 544 1574 544
Size: 1030 2500
BbeI G_GCGC'C
Cuts at: 964 1212 964
Size: 248 3282
BbsI GAAGACnn'nnnn_
Cuts at: 196 196
Size: 3530
BciVI GTATCCnnnnn_n'
Cuts at: 1428 2189 3530 1428
Size: 761 1341 1428
Fragments arranged by size:
1428 1341 761
BclI T'GATC_A
Cuts at: 1050 1050
Size: 3530
BglI GCCn_nnn'nGGC
Cuts at: 277 277
Size: 3530
BglII A'GATC_T
Cuts at: 1045 3082 1045
Size: 2037 1493
BlpI GC'TnA_GC
Cuts at: 642 642
Size: 3530
Bme1580I G_kGCm'C
Cuts at: 1155 1248 2304 1155
Size: 93 1056 2381
Fragments arranged by size:
2381 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 456 742 823 1158 3302 456
Size: 286 81 335 2144 684
Fragments arranged by size:
2144 684 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 706 1585 2077 2375 353
Size: 353 879 492 298 1508
Fragments arranged by size:
1508 879 492 353 298
BsaI GGTCTCn'nnnn_
Cuts at: 3234 3234
Size: 3530
BsaAI yAC'GTr
Cuts at: 883 883
Size: 3530
BsaBI GATnn'nnATC
Cuts at: 6 1044 3081 6
Size: 1038 2037 455
Fragments arranged by size:
2037 1038 455
BsaHI Gr'CG_yC
Cuts at: 961 1209 1911 3407 961
Size: 248 702 1496 1084
Fragments arranged by size:
1496 1084 702 248
BsaWI w'CCGG_w
Cuts at: 719 1240 1918 2192 2339 2750 719
Size: 521 678 274 147 411 1499
Fragments arranged by size:
1499 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 955 1713 2290 2786 955
Size: 758 577 496 1699
Fragments arranged by size:
1699 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1072 1118 2326 104
Size: 968 46 1208 1308
Fragments arranged by size:
1308 1208 968 46
BsiHKAI G_wGCw'C
Cuts at: 544 1322 1512 2304 544
Size: 778 190 792 1770
Fragments arranged by size:
1770 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 544 1155 1248 1322 1512 1574 2304 544
Size: 611 93 74 190 62 730 1770
Fragments arranged by size:
1770 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2750 2750
Size: 3530
BspHI T'CATG_A
Cuts at: 2706 2706
Size: 3530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 580 794 1096 1477 580
Size: 214 302 381 2633
Fragments arranged by size:
2633 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1822 1876 3360 3525 50
Size: 1772 54 1484 165 55
Fragments arranged by size:
1772 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1442 1442
Size: 3530
BsrFI r'CCGG_y
Cuts at: 1528 1709 1918 1528
Size: 181 209 3140
Fragments arranged by size:
3140 209 181
BssSI C'ACGA_G
Cuts at: 1801 2159 1801
Size: 358 3172
BstAPI GCAn_nnn'nTGC
Cuts at: 282 907 282
Size: 625 2905
BstBI TT'CG_AA
Cuts at: 1891 1891
Size: 3530
BstXI CCAn_nnnn'nTGG
Cuts at: 446 446
Size: 3530
BstYI r'GATC_y
Cuts at: 1 555 1022 1045 1380 1626 2638 2724 2736
Size: 554 467 23 335 246 1012 86 12
Cuts at: 2736 3082 1
Size: 346 449
Fragments arranged by size:
1012 554 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1480 1661 1824 1480
Size: 181 163 3186
Fragments arranged by size:
3186 181 163
BtsI GCAGTG_nn'
Cuts at: 773 773
Size: 3530
DraI TTT'AAA
Cuts at: 363 529 363
Size: 166 3364
DraIII CAC_nnn'GTG
Cuts at: 421 421
Size: 3530
DrdI GACnn_nn'nnGTC
Cuts at: 1236 2094 1236
Size: 858 2672
EaeI y'GGCC_r
Cuts at: 1115 1289 1680 1707 3052 1115
Size: 174 391 27 1345 1593
Fragments arranged by size:
1593 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1115 1115
Size: 3530
EarI CTCTTCn'nnn_
Cuts at: 1553 1763 1553
Size: 210 3320
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1738 2048 2194 3340 1738
Size: 310 146 1146 1928
Fragments arranged by size:
1928 1146 310 146
EcoICRI GAG'CTC
Cuts at: 542 542
Size: 3530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 1355 1787 2534 2802 455
Size: 900 432 747 268 1183
Fragments arranged by size:
1183 900 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 346 450 669 346
Size: 104 219 3207
Fragments arranged by size:
3207 219 104
EcoRI G'AATT_C
Cuts at: 204 330 534 204
Size: 126 204 3200
Fragments arranged by size:
3200 204 126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1549 1549
Size: 3530
FspI TGC'GCA
Cuts at: 1311 1311
Size: 3530
HaeII r_GCGC'y
Cuts at: 964 1212 2234 3213 964
Size: 248 1022 979 1281
Fragments arranged by size:
1281 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 397 429 1372 1402 1404 1434 397
Size: 32 943 30 2 30 2493
Fragments arranged by size:
2493 943 32 30 30 2
HincII GTy'rAC
Cuts at: 569 569
Size: 3530
KasI G'GCGC_C
Cuts at: 960 1208 960
Size: 248 3282
KpnI G_GTAC'C
Cuts at: 550 550
Size: 3530
MluI A'CGCG_T
Cuts at: 2963 2963
Size: 3530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 307 2201 2385 307
Size: 1894 184 1452
Fragments arranged by size:
1894 1452 184
MscI TGG'CCA
Cuts at: 1291 1291
Size: 3530
MslI CAynn'nnrTG
Cuts at: 1519 1646 1519
Size: 127 3403
MspA1I CmG'CkG
Cuts at: 268 641 955 1315 2328 2573 268
Size: 373 314 360 1013 245 1225
Fragments arranged by size:
1225 1013 373 360 314 245
NaeI GCC'GGC
Cuts at: 1711 1711
Size: 3530
NarI GG'CG_CC
Cuts at: 961 1209 961
Size: 248 3282
NcoI C'CATG_G
Cuts at: 107 107
Size: 3530
NgoMIV G'CCGG_C
Cuts at: 1709 1709
Size: 3530
NspI r_CATG'y
Cuts at: 583 1083 1990 583
Size: 500 907 2123
Fragments arranged by size:
2123 907 500
PciI A'CATG_T
Cuts at: 1986 1986
Size: 3530
PmeI GTTT'AAAC
Cuts at: 529 529
Size: 3530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 3498
PpuMI rG'GwC_Cy
Cuts at: 346 450 346
Size: 104 3426
PsiI TTA'TAA
Cuts at: 254 254
Size: 3530
PstI C_TGCA'G
Cuts at: 577 1262 577
Size: 685 2845
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3530
PvuII CAG'CTG
Cuts at: 268 955 1315 268
Size: 687 360 2483
Fragments arranged by size:
2483 687 360
RsrII CG'GwC_CG
Cuts at: 1725 1725
Size: 3530
SacI G_AGCT'C
Cuts at: 544 544
Size: 3530
SalI G'TCGA_C
Cuts at: 567 567
Size: 3530
SanDI GG'GwC_CC
Cuts at: 450 450
Size: 3530
SapI GCTCTTCn'nnn_
Cuts at: 1553 1763 1553
Size: 210 3320
SbfI CC_TGCA'GG
Cuts at: 577 577
Size: 3530
ScaI AGT'ACT
Cuts at: 3047 3047
Size: 3530
SfcI C'TryA_G
Cuts at: 33 573 1258 2251 2442 33
Size: 540 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 191
SfoI GGC'GCC
Cuts at: 962 1210 962
Size: 248 3282
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3530
SmaI CCC'GGG
Cuts at: 552 552
Size: 3530
SmlI C'TyrA_G
Cuts at: 368 685 1600 2092 2354 368
Size: 317 915 492 262 1544
Fragments arranged by size:
1544 915 492 317 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3530
SphI G_CATG'C
Cuts at: 583 1083 583
Size: 500 3030
StyI C'CwwG_G
Cuts at: 107 194 664 1018 107
Size: 87 470 354 2619
Fragments arranged by size:
2619 470 354 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 1113 1246 1705 1914 100
Size: 1013 133 459 209 1716
Fragments arranged by size:
1716 1013 459 209 133
TatI w'GTAC_w
Cuts at: 516 3045 516
Size: 2529 1001
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3498
Tth111I GACn'n_nGTC
Cuts at: 1327 1327
Size: 3530
XbaI T'CTAG_A
Cuts at: 148 561 148
Size: 413 3117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 443 1025 443
Size: 582 2948
XmaI C'CCGG_G
Cuts at: 550 550
Size: 3530
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI