Restriction map for
pF1KB8742
(Circular) (Six-base) MAPSORT of: pF1KB8742.seq Check: 2018 from: 1 to: 4238
pF1KB8742 4238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
AfeI AGC'GCT
Cuts at: 981 981
Size: 4238
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AhdI GACnn_n'nnGTC
Cuts at: 190 190
Size: 4238
AleI CACnn'nnGTG
Cuts at: 328 328
Size: 4238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 772 772
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 653 1144 1543 3110 653
Size: 491 399 1567 1781
Fragments arranged by size:
1781 1567 491 399
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 814 1242 3591 3847 814
Size: 428 2349 256 1205
Fragments arranged by size:
2349 1205 428 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1159 1258 1159
Size: 99 4139
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 1254 1668 1916 1951 1254
Size: 414 248 35 3541
Fragments arranged by size:
3541 414 248 35
BanII G_rGCy'C
Cuts at: 173 407 844 895 1252 2282 173
Size: 234 437 51 357 1030 2129
Fragments arranged by size:
2129 1030 437 357 234 51
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglII A'GATC_T
Cuts at: 578 1753 3790 578
Size: 1175 2037 1026
Fragments arranged by size:
2037 1175 1026
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 212 1863 1956 3012 212
Size: 1651 93 1056 1438
Fragments arranged by size:
1651 1438 1056 93
BmgBI CAC'GTC
Cuts at: 768 768
Size: 4238
BmrI ACTGGGnnnn_n'
Cuts at: 461 1035 1450 1531 1866 4010 461
Size: 574 415 81 335 2144 689
Fragments arranged by size:
2144 689 574 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1414 2293 2785 3083 157
Size: 1257 879 492 298 1312
Fragments arranged by size:
1312 1257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 628 896 3942 628
Size: 268 3046 924
Fragments arranged by size:
3046 924 268
BsaAI yAC'GTr
Cuts at: 822 1591 822
Size: 769 3469
BsaBI GATnn'nnATC
Cuts at: 6 577 583 1752 3789 6
Size: 571 6 1169 2037 455
Fragments arranged by size:
2037 1169 571 455 6
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 589 1427 1948 2626 2900 3047 3458 589
Size: 838 521 678 274 147 411 1369
Fragments arranged by size:
1369 838 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 811 841 919 949 811
Size: 30 78 30 4100
Fragments arranged by size:
4100 78 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 819 822 943 1177 784
Size: 35 3 121 234 3845
Fragments arranged by size:
3845 234 121 35 3
BseYI C'CCAG_C
Cuts at: 160 166 1663 2421 2998 3494 160
Size: 6 1497 758 577 496 904
Fragments arranged by size:
1497 904 758 577 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 1157 677
Size: 480 3758
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1252 2030 2220 3012 1252
Size: 778 190 792 2478
Fragments arranged by size:
2478 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 880 880
Size: 4238
Bsp1286I G_dGCh'C
Cuts at: 173 212 407 844 895 1252 1863 1956 2030
Size: 39 195 437 51 357 611 93 74
Cuts at: 2030 2220 2282 3012 173
Size: 190 62 730 1399
Fragments arranged by size:
1399 730 611 437 357 195 190 93
74 62 51 39
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 431 3414 431
Size: 2983 1255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 1288 1502 1804 2185 266
Size: 1022 214 302 381 2319
Fragments arranged by size:
2319 1022 381 302 214
BsrBI CCG'CTC
Cuts at: 50 786 2530 2584 4068 4233 50
Size: 736 1744 54 1484 165 55
Fragments arranged by size:
1744 1484 736 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1233 2150 1233
Size: 917 3321
BsrFI r'CCGG_y
Cuts at: 2236 2417 2626 2236
Size: 181 209 3848
Fragments arranged by size:
3848 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 1615 1615
Size: 4238
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstXI CCAn_nnnn'nTGG
Cuts at: 745 745
Size: 4238
BstYI r'GATC_y
Cuts at: 1 578 1263 1730 1753 2088 2334 3346 3432
Size: 577 685 467 23 335 246 1012 86
Cuts at: 3432 3444 3790 1
Size: 12 346 449
Fragments arranged by size:
1012 685 577 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 354 2188 2369 2532 354
Size: 1834 181 163 2060
Fragments arranged by size:
2060 1834 181 163
BtsI GCAGTG_nn'
Cuts at: 155 1481 155
Size: 1326 2912
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 250 285 250
Size: 35 4203
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 602 1823 1997 2388 2415 3760 602
Size: 1221 174 391 27 1345 1080
Fragments arranged by size:
1345 1221 1080 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 712 2261 2471 712
Size: 1549 210 2479
Fragments arranged by size:
2479 1549 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1377 1377
Size: 4238
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 705 741 2257 705
Size: 36 1516 2686
Fragments arranged by size:
2686 1516 36
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 983 1672 1920 2942 3921 983
Size: 689 248 1022 979 1300
Fragments arranged by size:
1300 1022 979 689 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 176 208 290 322 817 849 2080 2110 2112
Size: 32 82 32 495 32 1231 30 2
Cuts at: 2112 2142 176
Size: 30 2272
Fragments arranged by size:
2272 1231 495 82 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1277 1277
Size: 4238
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 604 1999 604
Size: 1395 2843
MslI CAynn'nnrTG
Cuts at: 127 328 436 2227 2354 127
Size: 201 108 1791 127 2011
Fragments arranged by size:
2011 1791 201 127 108
MspA1I CmG'CkG
Cuts at: 1349 1663 2023 3036 3281 1349
Size: 314 360 1013 245 2306
Fragments arranged by size:
2306 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NcoI C'CATG_G
Cuts at: 107 107
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NspI r_CATG'y
Cuts at: 132 1291 1791 2698 132
Size: 1159 500 907 1672
Fragments arranged by size:
1672 1159 907 500
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PflMI CCAn_nnn'nTGG
Cuts at: 879 879
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PmlI CAC'GTG
Cuts at: 822 822
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PshAI GACnn'nnGTC
Cuts at: 766 766
Size: 4238
PspXI vC'TCGA_Gb
Cuts at: 1159 1159
Size: 4238
PstI C_TGCA'G
Cuts at: 761 1285 1970 761
Size: 524 685 3029
Fragments arranged by size:
3029 685 524
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 712 2261 2471 712
Size: 1549 210 2479
Fragments arranged by size:
2479 1549 210
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SexAI A'CCwGG_T
Cuts at: 337 337
Size: 4238
SfcI C'TryA_G
Cuts at: 33 757 1281 1966 2959 3150 33
Size: 724 524 685 993 191 1121
Fragments arranged by size:
1121 993 724 685 524 191
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 172 1159 1393 2308 2800 3062 172
Size: 987 234 915 492 262 1348
Fragments arranged by size:
1348 987 915 492 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StyI C'CwwG_G
Cuts at: 107 773 1372 1726 107
Size: 666 599 354 2619
Fragments arranged by size:
2619 666 599 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 1954 2413 2622 1821
Size: 133 459 209 3437
Fragments arranged by size:
3437 459 209 133
TatI w'GTAC_w
Cuts at: 1169 3753 1169
Size: 2584 1654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 811 843 1061 1093 3755 3787 811
Size: 32 218 32 2662 32 1262
Fragments arranged by size:
2662 1262 218 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 487 540 1733 141
Size: 346 53 1193 2646
Fragments arranged by size:
2646 1193 346 53
XhoI C'TCGA_G
Cuts at: 1159 1159
Size: 4238
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
XmnI GAAnn'nnTTC
Cuts at: 1103 1103
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PshAI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PpuMI PsiI PspOMI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI