Restriction map for
pF1KB8743
(Circular) (Six-base) MAPSORT of: pF1KB8743.seq Check: 1455 from: 1 to: 3482
pF1KB8743 3482 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 520 520
Size: 3482
Acc65I G'GTAC_C
Cuts at: 498 498
Size: 3482
AclI AA'CG_TT
Cuts at: 3322 3322
Size: 3482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 1739 2486 2754 1307
Size: 432 747 268 2035
Fragments arranged by size:
2035 747 432 268
AfeI AGC'GCT
Cuts at: 346 346
Size: 3482
AflIII A'CryG_T
Cuts at: 834 1938 2915 834
Size: 1104 977 1401
Fragments arranged by size:
1401 1104 977
AgeI A'CCGG_T
Cuts at: 1870 1870
Size: 3482
AhdI GACnn_n'nnGTC
Cuts at: 278 278
Size: 3482
AlwNI CAG_nnn'CTG
Cuts at: 787 2354 787
Size: 1567 1915
ApaI G_GGCC'C
Cuts at: 397 397
Size: 3482
ApaLI G'TGCA_C
Cuts at: 2252 2252
Size: 3482
ApoI r'AATT_y
Cuts at: 486 2835 3091 486
Size: 2349 256 877
Fragments arranged by size:
2349 877 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3482
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
AvaI C'yCGr_G
Cuts at: 120 340 502 120
Size: 220 162 3100
Fragments arranged by size:
3100 220 162
BamHI G'GATC_C
Cuts at: 507 507
Size: 3482
BanI G'GyrC_C
Cuts at: 449 455 498 912 1160 1195 449
Size: 6 43 414 248 35 2736
Fragments arranged by size:
2736 414 248 43 35 6
BanII G_rGCy'C
Cuts at: 156 397 496 1526 156
Size: 241 99 1030 2112
Fragments arranged by size:
2112 1030 241 99
BbeI G_GCGC'C
Cuts at: 916 1164 916
Size: 248 3234
BciVI GTATCCnnnnn_n'
Cuts at: 1380 2141 3482 1380
Size: 761 1341 1380
Fragments arranged by size:
1380 1341 761
BclI T'GATC_A
Cuts at: 1002 1002
Size: 3482
BglI GCCn_nnn'nGGC
Cuts at: 124 124
Size: 3482
BglII A'GATC_T
Cuts at: 997 3034 997
Size: 2037 1445
BlpI GC'TnA_GC
Cuts at: 594 594
Size: 3482
Bme1580I G_kGCm'C
Cuts at: 397 460 1107 1200 2256 397
Size: 63 647 93 1056 1623
Fragments arranged by size:
1623 1056 647 93 63
BmgBI CAC'GTC
Cuts at: 228 228
Size: 3482
BmrI ACTGGGnnnn_n'
Cuts at: 148 694 775 1110 3254 148
Size: 546 81 335 2144 376
Fragments arranged by size:
2144 546 376 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1537 2029 2327 658
Size: 879 492 298 1813
Fragments arranged by size:
1813 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3186 3186
Size: 3482
BsaAI yAC'GTr
Cuts at: 835 835
Size: 3482
BsaBI GATnn'nnATC
Cuts at: 6 996 3033 6
Size: 990 2037 455
Fragments arranged by size:
2037 990 455
BsaHI Gr'CG_yC
Cuts at: 112 254 402 913 1161 1863 3359 112
Size: 142 148 511 248 702 1496 235
Fragments arranged by size:
1496 702 511 248 235 148 142
BsaWI w'CCGG_w
Cuts at: 671 1192 1870 2144 2291 2702 671
Size: 521 678 274 147 411 1451
Fragments arranged by size:
1451 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 282 282
Size: 3482
BseYI C'CCAG_C
Cuts at: 170 353 907 1665 2242 2738 170
Size: 183 554 758 577 496 914
Fragments arranged by size:
914 758 577 554 496 183
BsiEI CG_ry'CG
Cuts at: 104 160 468 1024 1070 2278 104
Size: 56 308 556 46 1208 1308
Fragments arranged by size:
1308 1208 556 308 56 46
BsiHKAI G_wGCw'C
Cuts at: 496 1274 1464 2256 496
Size: 778 190 792 1722
Fragments arranged by size:
1722 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 156 397 460 496 1107 1200 1274 1464 1526
Size: 241 63 36 611 93 74 190 62
Cuts at: 1526 2256 156
Size: 730 1382
Fragments arranged by size:
1382 730 611 241 190 93 74 63
62 36
BspEI T'CCGG_A
Cuts at: 2702 2702
Size: 3482
BspHI T'CATG_A
Cuts at: 2658 2658
Size: 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 532 746 1048 1429 532
Size: 214 302 381 2585
Fragments arranged by size:
2585 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1774 1828 3312 3477 50
Size: 1724 54 1484 165 55
Fragments arranged by size:
1724 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 1394
Size: 3482
BsrFI r'CCGG_y
Cuts at: 452 1480 1661 1870 452
Size: 1028 181 209 2064
Fragments arranged by size:
2064 1028 209 181
BssHII G'CGCG_C
Cuts at: 422 422
Size: 3482
BssSI C'ACGA_G
Cuts at: 1753 2111 1753
Size: 358 3124
BstAPI GCAn_nnn'nTGC
Cuts at: 859 859
Size: 3482
BstBI TT'CG_AA
Cuts at: 1843 1843
Size: 3482
BstYI r'GATC_y
Cuts at: 1 507 974 997 1332 1578 2590 2676 2688
Size: 506 467 23 335 246 1012 86 12
Cuts at: 2688 3034 1
Size: 346 449
Fragments arranged by size:
1012 506 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 128 160 397 128
Size: 32 237 3213
Fragments arranged by size:
3213 237 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1432 1613 1776 1432
Size: 181 163 3138
Fragments arranged by size:
3138 181 163
BtsI GCAGTG_nn'
Cuts at: 725 725
Size: 3482
DraI TTT'AAA
Cuts at: 481 481
Size: 3482
DrdI GACnn_nn'nnGTC
Cuts at: 1188 2046 1188
Size: 858 2624
EaeI y'GGCC_r
Cuts at: 157 1067 1241 1632 1659 3004 157
Size: 910 174 391 27 1345 635
Fragments arranged by size:
1345 910 635 391 174 27
EagI C'GGCC_G
Cuts at: 157 1067 157
Size: 910 2572
EarI CTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1690 2000 2146 3292 1690
Size: 310 146 1146 1880
Fragments arranged by size:
1880 1146 310 146
EcoICRI GAG'CTC
Cuts at: 494 494
Size: 3482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 1739 2486 2754 1307
Size: 432 747 268 2035
Fragments arranged by size:
2035 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 239 393 621 239
Size: 154 228 3100
Fragments arranged by size:
3100 228 154
EcoRI G'AATT_C
Cuts at: 486 486
Size: 3482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1501 1501
Size: 3482
FspI TGC'GCA
Cuts at: 1263 1263
Size: 3482
HaeII r_GCGC'y
Cuts at: 330 348 916 1164 2186 3165 330
Size: 18 568 248 1022 979 647
Fragments arranged by size:
1022 979 647 568 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 264 296 1324 1354 1356 1386 264
Size: 32 1028 30 2 30 2360
Fragments arranged by size:
2360 1028 32 30 30 2
HincII GTy'rAC
Cuts at: 521 521
Size: 3482
KasI G'GCGC_C
Cuts at: 912 1160 912
Size: 248 3234
KpnI G_GTAC'C
Cuts at: 502 502
Size: 3482
MluI A'CGCG_T
Cuts at: 2915 2915
Size: 3482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2337 2153
Size: 184 3298
MscI TGG'CCA
Cuts at: 1243 1243
Size: 3482
MslI CAynn'nnrTG
Cuts at: 1471 1598 1471
Size: 127 3355
MspA1I CmG'CkG
Cuts at: 162 170 399 593 907 1267 2280 2525 162
Size: 8 229 194 314 360 1013 245 1119
Fragments arranged by size:
1119 1013 360 314 245 229 194 8
NaeI GCC'GGC
Cuts at: 1663 1663
Size: 3482
NarI GG'CG_CC
Cuts at: 913 1161 913
Size: 248 3234
NgoMIV G'CCGG_C
Cuts at: 1661 1661
Size: 3482
NspI r_CATG'y
Cuts at: 434 535 1035 1942 434
Size: 101 500 907 1974
Fragments arranged by size:
1974 907 500 101
PciI A'CATG_T
Cuts at: 1938 1938
Size: 3482
PmeI GTTT'AAAC
Cuts at: 481 481
Size: 3482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2621 2653 2621
Size: 32 3450
PpuMI rG'GwC_Cy
Cuts at: 239 239
Size: 3482
PspOMI G'GGCC_C
Cuts at: 393 393
Size: 3482
PstI C_TGCA'G
Cuts at: 529 1214 529
Size: 685 2797
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3482
PvuII CAG'CTG
Cuts at: 907 1267 907
Size: 360 3122
RsrII CG'GwC_CG
Cuts at: 1677 1677
Size: 3482
SacI G_AGCT'C
Cuts at: 496 496
Size: 3482
SacII CC_GC'GG
Cuts at: 163 400 163
Size: 237 3245
SalI G'TCGA_C
Cuts at: 519 519
Size: 3482
SanDI GG'GwC_CC
Cuts at: 239 239
Size: 3482
SapI GCTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
SbfI CC_TGCA'GG
Cuts at: 529 529
Size: 3482
ScaI AGT'ACT
Cuts at: 2999 2999
Size: 3482
SfcI C'TryA_G
Cuts at: 33 417 525 1210 2203 2394 33
Size: 384 108 685 993 191 1121
Fragments arranged by size:
1121 993 685 384 191 108
SfoI GGC'GCC
Cuts at: 914 1162 914
Size: 248 3234
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
SmaI CCC'GGG
Cuts at: 504 504
Size: 3482
SmlI C'TyrA_G
Cuts at: 637 1552 2044 2306 637
Size: 915 492 262 1813
Fragments arranged by size:
1813 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3482
SphI G_CATG'C
Cuts at: 434 535 1035 434
Size: 101 500 2881
Fragments arranged by size:
2881 500 101
StyI C'CwwG_G
Cuts at: 616 970 616
Size: 354 3128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1065 1198 1657 1866 1065
Size: 133 459 209 2681
Fragments arranged by size:
2681 459 209 133
TatI w'GTAC_w
Cuts at: 2997 2997
Size: 3482
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3450
Tth111I GACn'n_nGTC
Cuts at: 1279 1279
Size: 3482
XbaI T'CTAG_A
Cuts at: 513 513
Size: 3482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 135 977 135
Size: 842 2640
XmaI C'CCGG_G
Cuts at: 502 502
Size: 3482
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI