Restriction map for
pF1KB8749
(Circular) (Six-base) MAPSORT of: pF1KB8749.seq Check: 6750 from: 1 to: 4367
pF1KB8749 4367 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 242 1405 242
Size: 1163 3204
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 2192 2624 3371 3639 548
Size: 1644 432 747 268 1276
Fragments arranged by size:
1644 1276 747 432 268
AflII C'TTAA_G
Cuts at: 573 573
Size: 4367
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AleI CACnn'nnGTG
Cuts at: 301 301
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 1253 1672 3239 1253
Size: 419 1567 2381
Fragments arranged by size:
2381 1567 419
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 1016 1371 3720 3976 1016
Size: 355 2349 256 1407
Fragments arranged by size:
2349 1407 355 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1010 1387 1010
Size: 377 3990
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 317 1383 1797 2045 2080 317
Size: 1066 414 248 35 2604
Fragments arranged by size:
2604 1066 414 248 35
BanII G_rGCy'C
Cuts at: 645 762 892 1381 2411 645
Size: 117 130 489 1030 2601
Fragments arranged by size:
2601 1030 489 130 117
BbeI G_GCGC'C
Cuts at: 321 1801 2049 321
Size: 1480 248 2639
Fragments arranged by size:
2639 1480 248
BbsI GAAGACnn'nnnn_
Cuts at: 213 788 213
Size: 575 3792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1175 1209 1175
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglI GCCn_nnn'nGGC
Cuts at: 622 622
Size: 4367
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1992 2085 3141 1992
Size: 93 1056 3218
Fragments arranged by size:
3218 1056 93
BmgBI CAC'GTC
Cuts at: 1199 1199
Size: 4367
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1036 2734 1036
Size: 1698 2669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2422 2914 3212 1543
Size: 879 492 298 2698
Fragments arranged by size:
2698 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 481 1264 4071 481
Size: 783 2807 777
Fragments arranged by size:
2807 783 777
BsaAI yAC'GTr
Cuts at: 738 1720 738
Size: 982 3385
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 318 1798 2046 2748 4244 318
Size: 1480 248 702 1496 441
Fragments arranged by size:
1496 1480 702 441 248
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1282 1282
Size: 4367
BseYI C'CCAG_C
Cuts at: 1792 2550 3127 3623 1792
Size: 758 577 496 2536
Fragments arranged by size:
2536 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1253 1179
Size: 74 4293
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 551 1381 2159 2349 3141 179
Size: 372 830 778 190 792 1405
Fragments arranged by size:
1405 830 792 778 372 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1209 1209
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 179 551 645 762 892 1381 1992 2085 2159
Size: 372 94 117 130 489 611 93 74
Cuts at: 2159 2349 2411 3141 179
Size: 190 62 730 1405
Fragments arranged by size:
1405 730 611 489 372 190 130 117
94 93 74 62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1417 1631 1933 2314 1417
Size: 214 302 381 3470
Fragments arranged by size:
3470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BssSI C'ACGA_G
Cuts at: 357 2638 2996 357
Size: 2281 358 1728
Fragments arranged by size:
2281 1728 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstEII G'GTnAC_C
Cuts at: 849 849
Size: 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 303 1137 303
Size: 834 3533
BstYI r'GATC_y
Cuts at: 1 1392 1859 1882 2217 2463 3475 3561 3573
Size: 1391 467 23 335 246 1012 86 12
Cuts at: 3573 3919 1
Size: 346 449
Fragments arranged by size:
1391 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1265 1265
Size: 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 1131 1610 1131
Size: 479 3888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 719 754 719
Size: 35 4332
DraI TTT'AAA
Cuts at: 813 1334 1366 813
Size: 521 32 3814
Fragments arranged by size:
3814 521 32
DraIII CAC_nnn'GTG
Cuts at: 314 439 314
Size: 125 4242
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 542 614 727 1952 2126 2517 2544 3889 542
Size: 72 113 1225 174 391 27 1345 1020
Fragments arranged by size:
1345 1225 1020 391 174 113 72 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 187 192 2390 2600 187
Size: 5 2198 210 1954
Fragments arranged by size:
2198 1954 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1272 2575 2885 3031 4177 1272
Size: 1303 310 146 1146 1462
Fragments arranged by size:
1462 1303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 548 2192 2624 3371 3639 547
Size: 1 1644 432 747 268 1275
Fragments arranged by size:
1644 1275 747 432 268 1
EcoO109I rG'GnC_Cy
Cuts at: 467 1506 467
Size: 1039 3328
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 321 1801 2049 3071 4050 321
Size: 1480 248 1022 979 638
Fragments arranged by size:
1480 1022 979 638 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2209 2239 2241 2271 2209
Size: 30 2 30 4305
Fragments arranged by size:
4305 30 30 2
HincII GTy'rAC
Cuts at: 285 444 831 1406 285
Size: 159 387 575 3246
Fragments arranged by size:
3246 575 387 159
HindIII A'AGCT_T
Cuts at: 694 694
Size: 4367
KasI G'GCGC_C
Cuts at: 317 1797 2045 317
Size: 1480 248 2639
Fragments arranged by size:
2639 1480 248
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MfeI C'AATT_G
Cuts at: 1135 1135
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1181 3038 3222 1181
Size: 1857 184 2326
Fragments arranged by size:
2326 1857 184
MscI TGG'CCA
Cuts at: 616 2128 616
Size: 1512 2855
MslI CAynn'nnrTG
Cuts at: 224 301 2356 2483 224
Size: 77 2055 127 2108
Fragments arranged by size:
2108 2055 127 77
MspA1I CmG'CkG
Cuts at: 233 1253 1478 1792 2152 3165 3410 233
Size: 1020 225 314 360 1013 245 1190
Fragments arranged by size:
1190 1020 1013 360 314 245 225
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 318 1798 2046 318
Size: 1480 248 2639
Fragments arranged by size:
2639 1480 248
NdeI CA'TA_TG
Cuts at: 323 323
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PmlI CAC'GTG
Cuts at: 738 738
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PshAI GACnn'nnGTC
Cuts at: 1221 1221
Size: 4367
PstI C_TGCA'G
Cuts at: 1414 2099 1414
Size: 685 3682
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 233 1253 1792 2152 233
Size: 1020 539 360 2448
Fragments arranged by size:
2448 1020 539 360
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 192 2390 2600 192
Size: 2198 210 1959
Fragments arranged by size:
2198 1959 210
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 1346 1410 2095 3088 3279 33
Size: 1313 64 685 993 191 1121
Fragments arranged by size:
1313 1121 993 685 191 64
SfoI GGC'GCC
Cuts at: 319 1799 2047 319
Size: 1480 248 2639
Fragments arranged by size:
2639 1480 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 573 1522 2437 2929 3191 573
Size: 949 915 492 262 1749
Fragments arranged by size:
1749 949 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 611 1208 1501 1855 611
Size: 597 293 354 3123
Fragments arranged by size:
3123 597 354 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1950 2083 2542 2751 1950
Size: 133 459 209 3566
Fragments arranged by size:
3566 459 209 133
TatI w'GTAC_w
Cuts at: 3882 3882
Size: 4367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 263 295 298 330 3884 3916 263
Size: 32 3 32 3554 32 714
Fragments arranged by size:
3554 714 32 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 448 2164 448
Size: 1716 2651
XbaI T'CTAG_A
Cuts at: 991 1398 991
Size: 407 3960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PmeI PmlI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstZ17I BtgI ClaI EcoNI EcoRV
FseI FspAI HpaI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI