Restriction map for
pF1KB8750
(Circular) (Six-base) MAPSORT of: pF1KB8750.seq Check: 5042 from: 1 to: 3533
pF1KB8750 3533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 176 332 571 176
Size: 156 239 3138
Fragments arranged by size:
3138 239 156
Acc65I G'GTAC_C
Cuts at: 549 549
Size: 3533
AclI AA'CG_TT
Cuts at: 3373 3373
Size: 3533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 1790 2537 2805 1358
Size: 432 747 268 2086
Fragments arranged by size:
2086 747 432 268
AflIII A'CryG_T
Cuts at: 885 1989 2966 885
Size: 1104 977 1452
Fragments arranged by size:
1452 1104 977
AgeI A'CCGG_T
Cuts at: 1921 1921
Size: 3533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 516 516
Size: 3533
AlwNI CAG_nnn'CTG
Cuts at: 838 2405 838
Size: 1567 1966
ApaLI G'TGCA_C
Cuts at: 2303 2303
Size: 3533
ApoI r'AATT_y
Cuts at: 537 2886 3142 537
Size: 2349 256 928
Fragments arranged by size:
2349 928 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
AvaI C'yCGr_G
Cuts at: 553 553
Size: 3533
BamHI G'GATC_C
Cuts at: 558 558
Size: 3533
BanI G'GyrC_C
Cuts at: 549 963 1211 1246 549
Size: 414 248 35 2836
Fragments arranged by size:
2836 414 248 35
BanII G_rGCy'C
Cuts at: 547 1577 547
Size: 1030 2503
BbeI G_GCGC'C
Cuts at: 967 1215 967
Size: 248 3285
BbvCI CC'TCA_GC
Cuts at: 158 158
Size: 3533
BciVI GTATCCnnnnn_n'
Cuts at: 1431 2192 3533 1431
Size: 761 1341 1431
Fragments arranged by size:
1431 1341 761
BclI T'GATC_A
Cuts at: 1053 1053
Size: 3533
BglII A'GATC_T
Cuts at: 1048 3085 1048
Size: 2037 1496
BlpI GC'TnA_GC
Cuts at: 645 645
Size: 3533
Bme1580I G_kGCm'C
Cuts at: 232 1158 1251 2307 232
Size: 926 93 1056 1458
Fragments arranged by size:
1458 1056 926 93
BmrI ACTGGGnnnn_n'
Cuts at: 528 745 826 1161 3305 528
Size: 217 81 335 2144 756
Fragments arranged by size:
2144 756 335 217 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3533
Bpu10I CC'TnA_GC
Cuts at: 158 158
Size: 3533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 709 1588 2080 2378 300
Size: 409 879 492 298 1455
Fragments arranged by size:
1455 879 492 409 298
BsaI GGTCTCn'nnnn_
Cuts at: 3237 3237
Size: 3533
BsaAI yAC'GTr
Cuts at: 886 886
Size: 3533
BsaBI GATnn'nnATC
Cuts at: 6 1047 3084 6
Size: 1041 2037 455
Fragments arranged by size:
2037 1041 455
BsaHI Gr'CG_yC
Cuts at: 964 1212 1914 3410 964
Size: 248 702 1496 1087
Fragments arranged by size:
1496 1087 702 248
BsaWI w'CCGG_w
Cuts at: 722 1243 1921 2195 2342 2753 722
Size: 521 678 274 147 411 1502
Fragments arranged by size:
1502 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 3533
BseYI C'CCAG_C
Cuts at: 469 958 1716 2293 2789 469
Size: 489 758 577 496 1213
Fragments arranged by size:
1213 758 577 496 489
BsiEI CG_ry'CG
Cuts at: 104 1075 1121 2329 104
Size: 971 46 1208 1308
Fragments arranged by size:
1308 1208 971 46
BsiHKAI G_wGCw'C
Cuts at: 547 1325 1515 2307 547
Size: 778 190 792 1773
Fragments arranged by size:
1773 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 232 547 1158 1251 1325 1515 1577 2307 232
Size: 315 611 93 74 190 62 730 1458
Fragments arranged by size:
1458 730 611 315 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2753 2753
Size: 3533
BspHI T'CATG_A
Cuts at: 348 2709 348
Size: 2361 1172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 583 797 1099 1480 583
Size: 214 302 381 2636
Fragments arranged by size:
2636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1825 1879 3363 3528 50
Size: 1775 54 1484 165 55
Fragments arranged by size:
1775 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 261 1445 261
Size: 1184 2349
BsrFI r'CCGG_y
Cuts at: 1531 1712 1921 1531
Size: 181 209 3143
Fragments arranged by size:
3143 209 181
BsrGI T'GTAC_A
Cuts at: 221 221
Size: 3533
BssSI C'ACGA_G
Cuts at: 1804 2162 1804
Size: 358 3175
BstAPI GCAn_nnn'nTGC
Cuts at: 910 910
Size: 3533
BstBI TT'CG_AA
Cuts at: 1894 1894
Size: 3533
BstYI r'GATC_y
Cuts at: 1 558 1025 1048 1383 1629 2641 2727 2739
Size: 557 467 23 335 246 1012 86 12
Cuts at: 2739 3085 1
Size: 346 449
Fragments arranged by size:
1012 557 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 188 374 107
Size: 81 186 3266
Fragments arranged by size:
3266 186 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1483 1664 1827 1483
Size: 181 163 3189
Fragments arranged by size:
3189 181 163
BtsI GCAGTG_nn'
Cuts at: 776 776
Size: 3533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 363 398 363
Size: 35 3498
DraI TTT'AAA
Cuts at: 532 532
Size: 3533
DraIII CAC_nnn'GTG
Cuts at: 482 482
Size: 3533
DrdI GACnn_nn'nnGTC
Cuts at: 1239 2097 1239
Size: 858 2675
EaeI y'GGCC_r
Cuts at: 1118 1292 1683 1710 3055 1118
Size: 174 391 27 1345 1596
Fragments arranged by size:
1596 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1118 1118
Size: 3533
EarI CTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1741 2051 2197 3343 1741
Size: 310 146 1146 1931
Fragments arranged by size:
1931 1146 310 146
EcoICRI GAG'CTC
Cuts at: 545 545
Size: 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 1790 2537 2805 1358
Size: 432 747 268 2086
Fragments arranged by size:
2086 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 672 672
Size: 3533
EcoRI G'AATT_C
Cuts at: 537 537
Size: 3533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1552 1552
Size: 3533
FspI TGC'GCA
Cuts at: 1314 1314
Size: 3533
HaeII r_GCGC'y
Cuts at: 967 1215 2237 3216 967
Size: 248 1022 979 1284
Fragments arranged by size:
1284 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1375 1405 1407 1437 1375
Size: 30 2 30 3471
Fragments arranged by size:
3471 30 30 2
HincII GTy'rAC
Cuts at: 572 572
Size: 3533
KasI G'GCGC_C
Cuts at: 963 1211 963
Size: 248 3285
KpnI G_GTAC'C
Cuts at: 553 553
Size: 3533
MluI A'CGCG_T
Cuts at: 2966 2966
Size: 3533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2388 2204
Size: 184 3349
MscI TGG'CCA
Cuts at: 1294 1294
Size: 3533
MslI CAynn'nnrTG
Cuts at: 187 1522 1649 187
Size: 1335 127 2071
Fragments arranged by size:
2071 1335 127
MspA1I CmG'CkG
Cuts at: 644 958 1318 2331 2576 644
Size: 314 360 1013 245 1601
Fragments arranged by size:
1601 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1714 1714
Size: 3533
NarI GG'CG_CC
Cuts at: 964 1212 964
Size: 248 3285
NcoI C'CATG_G
Cuts at: 107 188 107
Size: 81 3452
NgoMIV G'CCGG_C
Cuts at: 1712 1712
Size: 3533
NspI r_CATG'y
Cuts at: 586 1086 1993 586
Size: 500 907 2126
Fragments arranged by size:
2126 907 500
PciI A'CATG_T
Cuts at: 1989 1989
Size: 3533
PfoI T'CCnGG_A
Cuts at: 512 512
Size: 3533
PmeI GTTT'AAAC
Cuts at: 532 532
Size: 3533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 3501
PshAI GACnn'nnGTC
Cuts at: 376 376
Size: 3533
PstI C_TGCA'G
Cuts at: 151 580 1265 151
Size: 429 685 2419
Fragments arranged by size:
2419 685 429
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3533
PvuII CAG'CTG
Cuts at: 958 1318 958
Size: 360 3173
RsrII CG'GwC_CG
Cuts at: 1728 1728
Size: 3533
SacI G_AGCT'C
Cuts at: 547 547
Size: 3533
SalI G'TCGA_C
Cuts at: 570 570
Size: 3533
SapI GCTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
SbfI CC_TGCA'GG
Cuts at: 580 580
Size: 3533
ScaI AGT'ACT
Cuts at: 3050 3050
Size: 3533
SfcI C'TryA_G
Cuts at: 33 147 576 1261 2254 2445 33
Size: 114 429 685 993 191 1121
Fragments arranged by size:
1121 993 685 429 191 114
SfoI GGC'GCC
Cuts at: 965 1213 965
Size: 248 3285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
SmaI CCC'GGG
Cuts at: 555 555
Size: 3533
SmlI C'TyrA_G
Cuts at: 279 688 1603 2095 2357 279
Size: 409 915 492 262 1455
Fragments arranged by size:
1455 915 492 409 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3533
SphI G_CATG'C
Cuts at: 586 1086 586
Size: 500 3033
StuI AGG'CCT
Cuts at: 145 463 145
Size: 318 3215
StyI C'CwwG_G
Cuts at: 107 188 667 1021 107
Size: 81 479 354 2619
Fragments arranged by size:
2619 479 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 483 1116 1249 1708 1917 483
Size: 633 133 459 209 2099
Fragments arranged by size:
2099 633 459 209 133
TatI w'GTAC_w
Cuts at: 221 3048 221
Size: 2827 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3501
Tth111I GACn'n_nGTC
Cuts at: 1330 1330
Size: 3533
XbaI T'CTAG_A
Cuts at: 564 564
Size: 3533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1028 114
Size: 914 2619
XmaI C'CCGG_G
Cuts at: 553 553
Size: 3533
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI