Restriction map for
pF1KB8751
(Circular) (Six-base) MAPSORT of: pF1KB8751.seq Check: 1378 from: 1 to: 3665
pF1KB8751 3665 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 703 703
Size: 3665
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1922 2669 2937 1490
Size: 432 747 268 2218
Fragments arranged by size:
2218 747 432 268
AflIII A'CryG_T
Cuts at: 1017 2121 3098 1017
Size: 1104 977 1584
Fragments arranged by size:
1584 1104 977
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 480 970 2537 480
Size: 490 1567 1608
Fragments arranged by size:
1608 1567 490
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 250 261 337 511 669 3018 3274 250
Size: 11 76 174 158 2349 256 641
Fragments arranged by size:
2349 641 256 174 158 76 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 305 685 305
Size: 380 3285
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 681 1095 1343 1378 681
Size: 414 248 35 2968
Fragments arranged by size:
2968 414 248 35
BanII G_rGCy'C
Cuts at: 679 1709 679
Size: 1030 2635
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 1290 1383 2439 1290
Size: 93 1056 2516
Fragments arranged by size:
2516 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 2032 244
Size: 1788 1877
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 1018 1018
Size: 3665
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 588 854 1375 2053 2327 2474 2885 588
Size: 266 521 678 274 147 411 1368
Fragments arranged by size:
1368 678 521 411 274 266 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 606 606
Size: 3665
BseYI C'CCAG_C
Cuts at: 414 1090 1848 2425 2921 414
Size: 676 758 577 496 1158
Fragments arranged by size:
1158 758 676 577 496
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2461 104
Size: 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 679 1457 1647 2439 679
Size: 778 190 792 1905
Fragments arranged by size:
1905 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 679 1290 1383 1457 1647 1709 2439 679
Size: 611 93 74 190 62 730 1905
Fragments arranged by size:
1905 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 446 715 929 1231 1612 446
Size: 269 214 302 381 2499
Fragments arranged by size:
2499 381 302 269 214
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1577 1577
Size: 3665
BsrFI r'CCGG_y
Cuts at: 1663 1844 2053 1663
Size: 181 209 3275
Fragments arranged by size:
3275 209 181
BssSI C'ACGA_G
Cuts at: 1936 2294 1936
Size: 358 3307
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1042
Size: 3665
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstXI CCAn_nnnn'nTGG
Cuts at: 243 243
Size: 3665
BstYI r'GATC_y
Cuts at: 1 690 1157 1180 1515 1761 2773 2859 2871
Size: 689 467 23 335 246 1012 86 12
Cuts at: 2871 3217 1
Size: 346 449
Fragments arranged by size:
1012 689 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 193 908 193
Size: 715 2950
DraI TTT'AAA
Cuts at: 333 664 333
Size: 331 3334
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 177 255 568 1250 1424 1815 1842 3187 177
Size: 78 313 682 174 391 27 1345 655
Fragments arranged by size:
1345 682 655 391 313 174 78 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 131 1688 1898 131
Size: 1557 210 1898
Fragments arranged by size:
1898 1557 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2183 2329 3475 1873
Size: 310 146 1146 2063
Fragments arranged by size:
2063 1146 310 146
EcoICRI GAG'CTC
Cuts at: 677 677
Size: 3665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1490 1922 2669 2937 139
Size: 1351 432 747 268 867
Fragments arranged by size:
1351 867 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 804 804
Size: 3665
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 1099 1347 2369 3348 1099
Size: 248 1022 979 1416
Fragments arranged by size:
1416 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 1507 1537 1539 1569 487
Size: 32 988 30 2 30 2583
Fragments arranged by size:
2583 988 32 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 658 2336 2520 290
Size: 368 1678 184 1435
Fragments arranged by size:
1678 1435 368 184
MscI TGG'CCA
Cuts at: 179 570 1426 179
Size: 391 856 2418
Fragments arranged by size:
2418 856 391
MslI CAynn'nnrTG
Cuts at: 447 1654 1781 447
Size: 1207 127 2331
Fragments arranged by size:
2331 1207 127
MspA1I CmG'CkG
Cuts at: 327 776 1090 1450 2463 2708 327
Size: 449 314 360 1013 245 1284
Fragments arranged by size:
1284 1013 449 360 314 245
NaeI GCC'GGC
Cuts at: 1846 1846
Size: 3665
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NgoMIV G'CCGG_C
Cuts at: 1844 1844
Size: 3665
NspI r_CATG'y
Cuts at: 718 1218 2125 718
Size: 500 907 2258
Fragments arranged by size:
2258 907 500
PciI A'CATG_T
Cuts at: 2121 2121
Size: 3665
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PstI C_TGCA'G
Cuts at: 326 712 1397 326
Size: 386 685 2594
Fragments arranged by size:
2594 685 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 327 1090 1450 327
Size: 763 360 2542
Fragments arranged by size:
2542 763 360
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 679 679
Size: 3665
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 322 708 1393 2386 2577 33
Size: 289 386 685 993 191 1121
Fragments arranged by size:
1121 993 685 386 289 191
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2227 2489 820
Size: 915 492 262 1996
Fragments arranged by size:
1996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
SspI AAT'ATT
Cuts at: 197 197
Size: 3665
StyI C'CwwG_G
Cuts at: 799 1153 799
Size: 354 3311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1248 1381 1840 2049 1248
Size: 133 459 209 2864
Fragments arranged by size:
2864 459 209 133
TatI w'GTAC_w
Cuts at: 3180 3180
Size: 3665
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 3633
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 187 396 1160 187
Size: 209 764 2692
Fragments arranged by size:
2692 764 209
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI