Restriction map for
pF1KB8752
(Circular) (Six-base) MAPSORT of: pF1KB8752.seq Check: 4890 from: 1 to: 3683
pF1KB8752 3683 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 647 721 647
Size: 74 3609
Acc65I G'GTAC_C
Cuts at: 699 699
Size: 3683
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 301 988 2555 301
Size: 687 1567 1429
Fragments arranged by size:
1567 1429 687
ApaLI G'TGCA_C
Cuts at: 254 2453 254
Size: 2199 1484
ApoI r'AATT_y
Cuts at: 687 3036 3292 687
Size: 2349 256 1078
Fragments arranged by size:
2349 1078 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 703 703
Size: 3683
BamHI G'GATC_C
Cuts at: 708 708
Size: 3683
BanI G'GyrC_C
Cuts at: 699 1113 1361 1396 699
Size: 414 248 35 2986
Fragments arranged by size:
2986 414 248 35
BanII G_rGCy'C
Cuts at: 572 697 1727 572
Size: 125 1030 2528
Fragments arranged by size:
2528 1030 125
BbeI G_GCGC'C
Cuts at: 1117 1365 1117
Size: 248 3435
BbsI GAAGACnn'nnnn_
Cuts at: 423 423
Size: 3683
BciVI GTATCCnnnnn_n'
Cuts at: 405 1581 2342 3683 405
Size: 1176 761 1341 405
Fragments arranged by size:
1341 1176 761 405
BclI T'GATC_A
Cuts at: 1203 1203
Size: 3683
BglII A'GATC_T
Cuts at: 1198 3235 1198
Size: 2037 1646
BlpI GC'TnA_GC
Cuts at: 795 795
Size: 3683
Bme1580I G_kGCm'C
Cuts at: 258 1308 1401 2457 258
Size: 1050 93 1056 1484
Fragments arranged by size:
1484 1056 1050 93
BmrI ACTGGGnnnn_n'
Cuts at: 566 895 976 1311 3455 566
Size: 329 81 335 2144 794
Fragments arranged by size:
2144 794 335 329 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 3683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 416
Size: 3683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1738 2230 2528 859
Size: 879 492 298 2014
Fragments arranged by size:
2014 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 3387 340
Size: 3047 636
BsaAI yAC'GTr
Cuts at: 1036 1036
Size: 3683
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 1114 1362 2064 3560 1114
Size: 248 702 1496 1237
Fragments arranged by size:
1496 1237 702 248
BsaWI w'CCGG_w
Cuts at: 872 1393 2071 2345 2492 2903 872
Size: 521 678 274 147 411 1652
Fragments arranged by size:
1652 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 534 564 534
Size: 30 3653
BseYI C'CCAG_C
Cuts at: 1108 1866 2443 2939 1108
Size: 758 577 496 1852
Fragments arranged by size:
1852 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 606
Size: 3683
BsiEI CG_ry'CG
Cuts at: 104 1225 1271 2479 104
Size: 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 46
BsiHKAI G_wGCw'C
Cuts at: 258 697 1475 1665 2457 258
Size: 439 778 190 792 1484
Fragments arranged by size:
1484 792 778 439 190
Bsp1286I G_dGCh'C
Cuts at: 258 572 697 1308 1401 1475 1665 1727 2457
Size: 314 125 611 93 74 190 62 730
Cuts at: 2457 258
Size: 1484
Fragments arranged by size:
1484 730 611 314 190 125 93 74
62
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 733 947 1249 1630 733
Size: 214 302 381 2786
Fragments arranged by size:
2786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1975 2029 3513 3678 50
Size: 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 1595
Size: 3683
BsrFI r'CCGG_y
Cuts at: 1681 1862 2071 1681
Size: 181 209 3293
Fragments arranged by size:
3293 209 181
BssSI C'ACGA_G
Cuts at: 1954 2312 1954
Size: 358 3325
BstAPI GCAn_nnn'nTGC
Cuts at: 301 584 1060 301
Size: 283 476 2924
Fragments arranged by size:
2924 476 283
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstYI r'GATC_y
Cuts at: 1 708 1175 1198 1533 1779 2791 2877 2889
Size: 707 467 23 335 246 1012 86 12
Cuts at: 2889 3235 1
Size: 346 449
Fragments arranged by size:
1012 707 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 648 648
Size: 3683
BtgI C'CryG_G
Cuts at: 199 199
Size: 3683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 372 1633 1814 1977 372
Size: 1261 181 163 2078
Fragments arranged by size:
2078 1261 181 163
BtsI GCAGTG_nn'
Cuts at: 926 926
Size: 3683
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DraIII CAC_nnn'GTG
Cuts at: 584 584
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 132 1389 2247 132
Size: 1257 858 1568
Fragments arranged by size:
1568 1257 858
EaeI y'GGCC_r
Cuts at: 202 1268 1442 1833 1860 3205 202
Size: 1066 174 391 27 1345 680
Fragments arranged by size:
1345 1066 680 391 174 27
EagI C'GGCC_G
Cuts at: 1268 1268
Size: 3683
EarI CTCTTCn'nnn_
Cuts at: 189 429 1706 1916 189
Size: 240 1277 210 1956
Fragments arranged by size:
1956 1277 240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1891 2201 2347 3493 1891
Size: 310 146 1146 2081
Fragments arranged by size:
2081 1146 310 146
EcoICRI GAG'CTC
Cuts at: 695 695
Size: 3683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 1508 1940 2687 2955 416
Size: 1092 432 747 268 1144
Fragments arranged by size:
1144 1092 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 632 822 632
Size: 190 3493
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 440 1702 440
Size: 1262 2421
FspI TGC'GCA
Cuts at: 1464 1464
Size: 3683
HaeII r_GCGC'y
Cuts at: 1117 1365 2387 3366 1117
Size: 248 1022 979 1434
Fragments arranged by size:
1434 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1525 1555 1557 1587 1525
Size: 30 2 30 3621
Fragments arranged by size:
3621 30 30 2
HincII GTy'rAC
Cuts at: 722 722
Size: 3683
KasI G'GCGC_C
Cuts at: 1113 1361 1113
Size: 248 3435
KpnI G_GTAC'C
Cuts at: 703 703
Size: 3683
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 677 2354 2538 677
Size: 1677 184 1822
Fragments arranged by size:
1822 1677 184
MscI TGG'CCA
Cuts at: 204 1444 204
Size: 1240 2443
MslI CAynn'nnrTG
Cuts at: 1672 1799 1672
Size: 127 3556
MspA1I CmG'CkG
Cuts at: 794 1108 1468 2481 2726 794
Size: 314 360 1013 245 1751
Fragments arranged by size:
1751 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1864 1864
Size: 3683
NarI GG'CG_CC
Cuts at: 1114 1362 1114
Size: 248 3435
NcoI C'CATG_G
Cuts at: 199 199
Size: 3683
NgoMIV G'CCGG_C
Cuts at: 1862 1862
Size: 3683
NspI r_CATG'y
Cuts at: 736 1236 2143 736
Size: 500 907 2276
Fragments arranged by size:
2276 907 500
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PflMI CCAn_nnn'nTGG
Cuts at: 533 533
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PpuMI rG'GwC_Cy
Cuts at: 632 632
Size: 3683
PstI C_TGCA'G
Cuts at: 730 1415 730
Size: 685 2998
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 1108 1468 1108
Size: 360 3323
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 697 697
Size: 3683
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
SbfI CC_TGCA'GG
Cuts at: 730 730
Size: 3683
ScaI AGT'ACT
Cuts at: 3200 3200
Size: 3683
SfcI C'TryA_G
Cuts at: 33 641 726 1411 2404 2595 33
Size: 608 85 685 993 191 1121
Fragments arranged by size:
1121 993 685 608 191 85
SfoI GGC'GCC
Cuts at: 1115 1363 1115
Size: 248 3435
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SmaI CCC'GGG
Cuts at: 705 705
Size: 3683
SmlI C'TyrA_G
Cuts at: 838 1753 2245 2507 838
Size: 915 492 262 2014
Fragments arranged by size:
2014 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 736 1236 736
Size: 500 3183
StuI AGG'CCT
Cuts at: 215 215
Size: 3683
StyI C'CwwG_G
Cuts at: 199 216 817 1171 199
Size: 17 601 354 2711
Fragments arranged by size:
2711 601 354 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 222 244 1266 1399 1858 2067 222
Size: 22 1022 133 459 209 1838
Fragments arranged by size:
1838 1022 459 209 133 22
TatI w'GTAC_w
Cuts at: 3198 3198
Size: 3683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 534 566 3200 3232 219
Size: 32 283 32 2634 32 670
Fragments arranged by size:
2634 670 283 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 714 714
Size: 3683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1178 1178
Size: 3683
XmaI C'CCGG_G
Cuts at: 703 703
Size: 3683
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI