Restriction map for
pF1KB8753
(Circular) (Six-base) MAPSORT of: pF1KB8753.seq Check: 1449 from: 1 to: 3701
pF1KB8753 3701 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 739 739
Size: 3701
Acc65I G'GTAC_C
Cuts at: 717 717
Size: 3701
AclI AA'CG_TT
Cuts at: 3541 3541
Size: 3701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1526 1958 2705 2973 223
Size: 1303 432 747 268 951
Fragments arranged by size:
1303 951 747 432 268
AflII C'TTAA_G
Cuts at: 505 505
Size: 3701
AflIII A'CryG_T
Cuts at: 275 1053 2157 3134 275
Size: 778 1104 977 842
Fragments arranged by size:
1104 977 842 778
AgeI A'CCGG_T
Cuts at: 2089 2089
Size: 3701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 664 664
Size: 3701
AlwNI CAG_nnn'CTG
Cuts at: 612 1006 2573 612
Size: 394 1567 1740
Fragments arranged by size:
1740 1567 394
ApaLI G'TGCA_C
Cuts at: 2471 2471
Size: 3701
ApoI r'AATT_y
Cuts at: 120 705 3054 3310 120
Size: 585 2349 256 511
Fragments arranged by size:
2349 585 511 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
AvaI C'yCGr_G
Cuts at: 397 595 721 397
Size: 198 126 3377
Fragments arranged by size:
3377 198 126
BamHI G'GATC_C
Cuts at: 726 726
Size: 3701
BanI G'GyrC_C
Cuts at: 483 717 1131 1379 1414 483
Size: 234 414 248 35 2770
Fragments arranged by size:
2770 414 248 234 35
BanII G_rGCy'C
Cuts at: 416 560 663 715 1745 416
Size: 144 103 52 1030 2372
Fragments arranged by size:
2372 1030 144 103 52
BbeI G_GCGC'C
Cuts at: 1135 1383 1135
Size: 248 3453
BciVI GTATCCnnnnn_n'
Cuts at: 1599 2360 3701 1599
Size: 761 1341 1599
Fragments arranged by size:
1599 1341 761
BclI T'GATC_A
Cuts at: 1221 1221
Size: 3701
BglI GCCn_nnn'nGGC
Cuts at: 667 667
Size: 3701
BglII A'GATC_T
Cuts at: 691 1216 3253 691
Size: 525 2037 1139
Fragments arranged by size:
2037 1139 525
BlpI GC'TnA_GC
Cuts at: 410 671 813 410
Size: 261 142 3298
Fragments arranged by size:
3298 261 142
Bme1580I G_kGCm'C
Cuts at: 275 576 1326 1419 2475 275
Size: 301 750 93 1056 1501
Fragments arranged by size:
1501 1056 750 301 93
BmrI ACTGGGnnnn_n'
Cuts at: 590 913 994 1329 3473 590
Size: 323 81 335 2144 818
Fragments arranged by size:
2144 818 335 323 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1756 2248 2546 877
Size: 879 492 298 2032
Fragments arranged by size:
2032 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 3405 217
Size: 3188 513
BsaAI yAC'GTr
Cuts at: 276 366 1054 276
Size: 90 688 2923
Fragments arranged by size:
2923 688 90
BsaBI GATnn'nnATC
Cuts at: 6 1215 3252 6
Size: 1209 2037 455
Fragments arranged by size:
2037 1209 455
BsaHI Gr'CG_yC
Cuts at: 449 1132 1380 2082 3578 449
Size: 683 248 702 1496 572
Fragments arranged by size:
1496 702 683 572 248
BsaWI w'CCGG_w
Cuts at: 890 1411 2089 2363 2510 2921 890
Size: 521 678 274 147 411 1670
Fragments arranged by size:
1670 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 551 1126 1884 2461 2957 551
Size: 575 758 577 496 1295
Fragments arranged by size:
1295 758 577 575 496
BsiEI CG_ry'CG
Cuts at: 104 1243 1289 2497 104
Size: 1139 46 1208 1308
Fragments arranged by size:
1308 1208 1139 46
BsiHKAI G_wGCw'C
Cuts at: 416 560 715 1493 1683 2475 416
Size: 144 155 778 190 792 1642
Fragments arranged by size:
1642 792 778 190 155 144
Bsp1286I G_dGCh'C
Cuts at: 275 416 560 576 663 715 1326 1419 1493
Size: 141 144 16 87 52 611 93 74
Cuts at: 1493 1683 1745 2475 275
Size: 190 62 730 1501
Fragments arranged by size:
1501 730 611 190 144 141 93 87
74 62 52 16
BspEI T'CCGG_A
Cuts at: 2921 2921
Size: 3701
BspHI T'CATG_A
Cuts at: 2877 2877
Size: 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 751 965 1267 1648 751
Size: 214 302 381 2804
Fragments arranged by size:
2804 381 302 214
BsrBI CCG'CTC
Cuts at: 50 315 1993 2047 3531 3696 50
Size: 265 1678 54 1484 165 55
Fragments arranged by size:
1678 1484 265 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1613 1613
Size: 3701
BsrFI r'CCGG_y
Cuts at: 1699 1880 2089 1699
Size: 181 209 3311
Fragments arranged by size:
3311 209 181
BssSI C'ACGA_G
Cuts at: 358 1972 2330 358
Size: 1614 358 1729
Fragments arranged by size:
1729 1614 358
BstAPI GCAn_nnn'nTGC
Cuts at: 490 1078 490
Size: 588 3113
BstBI TT'CG_AA
Cuts at: 2062 2062
Size: 3701
BstYI r'GATC_y
Cuts at: 1 691 726 1193 1216 1551 1797 2809 2895
Size: 690 35 467 23 335 246 1012 86
Cuts at: 2895 2907 3253 1
Size: 12 346 449
Fragments arranged by size:
1012 690 467 449 346 335 246 86
35 23 12
BtgI C'CryG_G
Cuts at: 107 663 107
Size: 556 3145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1651 1832 1995 1651
Size: 181 163 3357
Fragments arranged by size:
3357 181 163
BtsI GCAGTG_nn'
Cuts at: 576 944 576
Size: 368 3333
DraI TTT'AAA
Cuts at: 700 700
Size: 3701
DrdI GACnn_nn'nnGTC
Cuts at: 1407 2265 1407
Size: 858 2843
EaeI y'GGCC_r
Cuts at: 467 1286 1460 1851 1878 3223 467
Size: 819 174 391 27 1345 945
Fragments arranged by size:
1345 945 819 391 174 27
EagI C'GGCC_G
Cuts at: 1286 1286
Size: 3701
EarI CTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 1909 2219 2365 3511 154
Size: 1755 310 146 1146 344
Fragments arranged by size:
1755 1146 344 310 146
EcoICRI GAG'CTC
Cuts at: 414 558 713 414
Size: 144 155 3402
Fragments arranged by size:
3402 155 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1526 1958 2705 2973 223
Size: 1303 432 747 268 951
Fragments arranged by size:
1303 951 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 309 600 840 309
Size: 291 240 3170
Fragments arranged by size:
3170 291 240
EcoRI G'AATT_C
Cuts at: 120 705 120
Size: 585 3116
EcoRV GAT'ATC
Cuts at: 354 354
Size: 3701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1720 1720
Size: 3701
FspI TGC'GCA
Cuts at: 1482 1482
Size: 3701
HaeII r_GCGC'y
Cuts at: 1135 1383 2405 3384 1135
Size: 248 1022 979 1452
Fragments arranged by size:
1452 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1543 1573 1575 1605 1543
Size: 30 2 30 3639
Fragments arranged by size:
3639 30 30 2
HincII GTy'rAC
Cuts at: 740 740
Size: 3701
KasI G'GCGC_C
Cuts at: 1131 1379 1131
Size: 248 3453
KpnI G_GTAC'C
Cuts at: 721 721
Size: 3701
MluI A'CGCG_T
Cuts at: 3134 3134
Size: 3701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2556 2372
Size: 184 3517
MscI TGG'CCA
Cuts at: 469 1462 469
Size: 993 2708
MslI CAynn'nnrTG
Cuts at: 1690 1817 1690
Size: 127 3574
MspA1I CmG'CkG
Cuts at: 216 326 501 551 638 812 1126 1486 2499
Size: 110 175 50 87 174 314 360 1013
Cuts at: 2499 2744 216
Size: 245 1173
Fragments arranged by size:
1173 1013 360 314 245 175 174 110
87 50
NaeI GCC'GGC
Cuts at: 1882 1882
Size: 3701
NarI GG'CG_CC
Cuts at: 1132 1380 1132
Size: 248 3453
NcoI C'CATG_G
Cuts at: 107 663 107
Size: 556 3145
NgoMIV G'CCGG_C
Cuts at: 1880 1880
Size: 3701
NspI r_CATG'y
Cuts at: 645 754 1254 2161 645
Size: 109 500 907 2185
Fragments arranged by size:
2185 907 500 109
PciI A'CATG_T
Cuts at: 2157 2157
Size: 3701
PflMI CCAn_nnn'nTGG
Cuts at: 216 219 216
Size: 3 3698
PfoI T'CCnGG_A
Cuts at: 539 539
Size: 3701
PmeI GTTT'AAAC
Cuts at: 700 700
Size: 3701
PmlI CAC'GTG
Cuts at: 276 276
Size: 3701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 3669
PstI C_TGCA'G
Cuts at: 571 608 653 748 1433 571
Size: 37 45 95 685 2839
Fragments arranged by size:
2839 685 95 45 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3701
PvuII CAG'CTG
Cuts at: 216 326 638 1126 1486 216
Size: 110 312 488 360 2431
Fragments arranged by size:
2431 488 360 312 110
RsrII CG'GwC_CG
Cuts at: 1896 1896
Size: 3701
SacI G_AGCT'C
Cuts at: 416 560 715 416
Size: 144 155 3402
Fragments arranged by size:
3402 155 144
SalI G'TCGA_C
Cuts at: 738 738
Size: 3701
SapI GCTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
SbfI CC_TGCA'GG
Cuts at: 748 748
Size: 3701
ScaI AGT'ACT
Cuts at: 3218 3218
Size: 3701
SfcI C'TryA_G
Cuts at: 33 567 604 649 744 1429 2422 2613 33
Size: 534 37 45 95 685 993 191 1121
Fragments arranged by size:
1121 993 685 534 191 95 45 37
SfoI GGC'GCC
Cuts at: 1133 1381 1133
Size: 248 3453
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
SmaI CCC'GGG
Cuts at: 723 723
Size: 3701
SmlI C'TyrA_G
Cuts at: 505 856 1771 2263 2525 505
Size: 351 915 492 262 1681
Fragments arranged by size:
1681 915 492 351 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3701
SphI G_CATG'C
Cuts at: 754 1254 754
Size: 500 3201
StyI C'CwwG_G
Cuts at: 107 663 835 1189 107
Size: 556 172 354 2619
Fragments arranged by size:
2619 556 354 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 1417 1876 2085 1284
Size: 133 459 209 2900
Fragments arranged by size:
2900 459 209 133
TatI w'GTAC_w
Cuts at: 3216 3216
Size: 3701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 3669
Tth111I GACn'n_nGTC
Cuts at: 1498 1498
Size: 3701
XbaI T'CTAG_A
Cuts at: 528 732 528
Size: 204 3497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1196 1196
Size: 3701
XmaI C'CCGG_G
Cuts at: 721 721
Size: 3701
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI