Restriction map for
pF1KB8754
(Circular) (Six-base) MAPSORT of: pF1KB8754.seq Check: 2939 from: 1 to: 3707
pF1KB8754 3707 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 234 234
Size: 3707
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AfeI AGC'GCT
Cuts at: 649 649
Size: 3707
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 327 387 597 1012 2579 327
Size: 60 210 415 1567 1455
Fragments arranged by size:
1567 1455 415 210 60
ApaI G_GGCC'C
Cuts at: 404 404
Size: 3707
ApaLI G'TGCA_C
Cuts at: 270 2477 270
Size: 2207 1500
ApoI r'AATT_y
Cuts at: 711 3060 3316 711
Size: 2349 256 1102
Fragments arranged by size:
2349 1102 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 727 727
Size: 3707
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 723 1137 1385 1420 723
Size: 414 248 35 3010
Fragments arranged by size:
3010 414 248 35
BanII G_rGCy'C
Cuts at: 172 404 587 721 1751 172
Size: 232 183 134 1030 2128
Fragments arranged by size:
2128 1030 232 183 134
BbeI G_GCGC'C
Cuts at: 1141 1389 1141
Size: 248 3459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3673
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 248 1227 248
Size: 979 2728
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 199 274 404 1332 1425 2481 199
Size: 75 130 928 93 1056 1425
Fragments arranged by size:
1425 1056 928 130 93 75
BmrI ACTGGGnnnn_n'
Cuts at: 919 1000 1335 3479 919
Size: 81 335 2144 1147
Fragments arranged by size:
2144 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 628 409
Size: 219 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1762 2254 2552 883
Size: 879 492 298 2038
Fragments arranged by size:
2038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 544 3411 544
Size: 2867 840
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 1138 1386 2088 3584 1138
Size: 248 702 1496 1261
Fragments arranged by size:
1496 1261 702 248
BsaWI w'CCGG_w
Cuts at: 896 1417 2095 2369 2516 2927 896
Size: 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 520 550 520
Size: 30 3677
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 406
Size: 3707
BseYI C'CCAG_C
Cuts at: 396 1132 1890 2467 2963 396
Size: 736 758 577 496 1140
Fragments arranged by size:
1140 758 736 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 3707
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 172 274 587 721 1499 1689 2481 172
Size: 102 313 134 778 190 792 1398
Fragments arranged by size:
1398 792 778 313 190 134 102
BsmBI CGTCTCn'nnnn_
Cuts at: 475 604 475
Size: 129 3578
Bsp1286I G_dGCh'C
Cuts at: 172 199 274 404 587 721 1332 1425 1499
Size: 27 75 130 183 134 611 93 74
Cuts at: 1499 1689 1751 2481 172
Size: 190 62 730 1398
Fragments arranged by size:
1398 730 611 190 183 134 130 93
75 74 62 27
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 234 757 971 1273 1654 234
Size: 523 214 302 381 2287
Fragments arranged by size:
2287 523 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1999 2053 3537 3702 50
Size: 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1619 1619
Size: 3707
BsrFI r'CCGG_y
Cuts at: 239 295 1705 1886 2095 239
Size: 56 1410 181 209 1851
Fragments arranged by size:
1851 1410 209 181 56
BssSI C'ACGA_G
Cuts at: 460 1978 2336 460
Size: 1518 358 1831
Fragments arranged by size:
1831 1518 358
BstAPI GCAn_nnn'nTGC
Cuts at: 473 1084 473
Size: 611 3096
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstYI r'GATC_y
Cuts at: 1 732 1199 1222 1557 1803 2815 2901 2913
Size: 731 467 23 335 246 1012 86 12
Cuts at: 2913 3259 1
Size: 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 491 1657 1838 2001 491
Size: 1166 181 163 2197
Fragments arranged by size:
2197 1166 181 163
BtsI GCAGTG_nn'
Cuts at: 950 950
Size: 3707
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 314 557 1292 1466 1857 1884 3229 314
Size: 243 735 174 391 27 1345 792
Fragments arranged by size:
1345 792 735 391 243 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 592 658 1730 1940 592
Size: 66 1072 210 2359
Fragments arranged by size:
2359 1072 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 170 585 719 170
Size: 415 134 3158
Fragments arranged by size:
3158 415 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 628 1532 1964 2711 2979 409
Size: 219 904 432 747 268 1137
Fragments arranged by size:
1137 904 747 432 268 219
EcoO109I rG'GnC_Cy
Cuts at: 400 846 400
Size: 446 3261
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 1726 603
Size: 1123 2584
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 327 651 1141 1389 2411 3390 327
Size: 324 490 248 1022 979 644
Fragments arranged by size:
1022 979 644 490 324 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1549 1579 1581 1611 1549
Size: 30 2 30 3645
Fragments arranged by size:
3645 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
KasI G'GCGC_C
Cuts at: 1137 1385 1137
Size: 248 3459
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2562 2378
Size: 184 3523
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 358 451 697 1696 1823 358
Size: 93 246 999 127 2242
Fragments arranged by size:
2242 999 246 127 93
MspA1I CmG'CkG
Cuts at: 254 489 818 1132 1492 2505 2750 254
Size: 235 329 314 360 1013 245 1211
Fragments arranged by size:
1211 1013 360 329 314 245 235
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 1138 1386 1138
Size: 248 3459
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PfoI T'CCnGG_A
Cuts at: 590 652 590
Size: 62 3645
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PspOMI G'GGCC_C
Cuts at: 400 400
Size: 3707
PstI C_TGCA'G
Cuts at: 281 754 1439 281
Size: 473 685 2549
Fragments arranged by size:
2549 685 473
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 254 1132 1492 254
Size: 878 360 2469
Fragments arranged by size:
2469 878 360
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 172 587 721 172
Size: 415 134 3158
Fragments arranged by size:
3158 415 134
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 592 1730 1940 592
Size: 1138 210 2359
Fragments arranged by size:
2359 1138 210
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 277 750 1435 2428 2619 33
Size: 244 473 685 993 191 1121
Fragments arranged by size:
1121 993 685 473 244 191
SfoI GGC'GCC
Cuts at: 1139 1387 1139
Size: 248 3459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 862 1777 2269 2531 862
Size: 915 492 262 2038
Fragments arranged by size:
2038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
StyI C'CwwG_G
Cuts at: 338 841 1195 338
Size: 503 354 2850
Fragments arranged by size:
2850 503 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1423 1882 2091 1290
Size: 133 459 209 2906
Fragments arranged by size:
2906 459 209 133
TatI w'GTAC_w
Cuts at: 3222 3222
Size: 3707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 520 552 3224 3256 520
Size: 32 2672 32 971
Fragments arranged by size:
2672 971 32 32
Tth111I GACn'n_nGTC
Cuts at: 676 1504 676
Size: 828 2879
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI