Restriction map for
pF1KB8755
(Circular) (Six-base) MAPSORT of: pF1KB8755.seq Check: 7374 from: 1 to: 3710
pF1KB8755 3710 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 748 748
Size: 3710
Acc65I G'GTAC_C
Cuts at: 726 726
Size: 3710
AclI AA'CG_TT
Cuts at: 3550 3550
Size: 3710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1535 1967 2714 2982 1535
Size: 432 747 268 2263
Fragments arranged by size:
2263 747 432 268
AflIII A'CryG_T
Cuts at: 1062 2166 3143 1062
Size: 1104 977 1629
Fragments arranged by size:
1629 1104 977
AgeI A'CCGG_T
Cuts at: 2098 2098
Size: 3710
AhdI GACnn_n'nnGTC
Cuts at: 629 629
Size: 3710
AlwNI CAG_nnn'CTG
Cuts at: 327 600 1015 2582 327
Size: 273 415 1567 1455
Fragments arranged by size:
1567 1455 415 273
ApaLI G'TGCA_C
Cuts at: 2480 2480
Size: 3710
ApoI r'AATT_y
Cuts at: 714 3063 3319 714
Size: 2349 256 1105
Fragments arranged by size:
2349 1105 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3710
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
AvaI C'yCGr_G
Cuts at: 730 730
Size: 3710
BamHI G'GATC_C
Cuts at: 735 735
Size: 3710
BanI G'GyrC_C
Cuts at: 726 1140 1388 1423 726
Size: 414 248 35 3013
Fragments arranged by size:
3013 414 248 35
BanII G_rGCy'C
Cuts at: 172 398 487 724 1754 172
Size: 226 89 237 1030 2128
Fragments arranged by size:
2128 1030 237 226 89
BbeI G_GCGC'C
Cuts at: 1144 1392 1144
Size: 248 3462
BbsI GAAGACnn'nnnn_
Cuts at: 236 236
Size: 3710
BbvCI CC'TCA_GC
Cuts at: 558 558
Size: 3710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3676
BciVI GTATCCnnnnn_n'
Cuts at: 1608 2369 3710 1608
Size: 761 1341 1608
Fragments arranged by size:
1608 1341 761
BclI T'GATC_A
Cuts at: 248 698 1230 248
Size: 450 532 2728
Fragments arranged by size:
2728 532 450
BglII A'GATC_T
Cuts at: 1225 3262 1225
Size: 2037 1673
BlpI GC'TnA_GC
Cuts at: 822 822
Size: 3710
Bme1580I G_kGCm'C
Cuts at: 347 1335 1428 2484 347
Size: 988 93 1056 1573
Fragments arranged by size:
1573 1056 988 93
BmgBI CAC'GTC
Cuts at: 438 438
Size: 3710
BmrI ACTGGGnnnn_n'
Cuts at: 922 1003 1338 3482 922
Size: 81 335 2144 1150
Fragments arranged by size:
2144 1150 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 3710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 631 619
Size: 12 3698
Bpu10I CC'TnA_GC
Cuts at: 113 558 113
Size: 445 3265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 886 1765 2257 2555 382
Size: 504 879 492 298 1537
Fragments arranged by size:
1537 879 504 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 547 3414 547
Size: 2867 843
BsaAI yAC'GTr
Cuts at: 1063 1063
Size: 3710
BsaBI GATnn'nnATC
Cuts at: 6 1224 3261 6
Size: 1218 2037 455
Fragments arranged by size:
2037 1218 455
BsaHI Gr'CG_yC
Cuts at: 1141 1389 2091 3587 1141
Size: 248 702 1496 1264
Fragments arranged by size:
1496 1264 702 248
BsaWI w'CCGG_w
Cuts at: 899 1420 2098 2372 2519 2930 899
Size: 521 678 274 147 411 1679
Fragments arranged by size:
1679 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 406
Size: 3710
BseYI C'CCAG_C
Cuts at: 487 1135 1893 2470 2966 487
Size: 648 758 577 496 1231
Fragments arranged by size:
1231 758 648 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 3710
BsiEI CG_ry'CG
Cuts at: 104 1252 1298 2506 104
Size: 1148 46 1208 1308
Fragments arranged by size:
1308 1208 1148 46
BsiHKAI G_wGCw'C
Cuts at: 172 398 724 1502 1692 2484 172
Size: 226 326 778 190 792 1398
Fragments arranged by size:
1398 792 778 326 226 190
Bsp1286I G_dGCh'C
Cuts at: 172 347 398 487 724 1335 1428 1502 1692
Size: 175 51 89 237 611 93 74 190
Cuts at: 1692 1754 2484 172
Size: 62 730 1398
Fragments arranged by size:
1398 730 611 237 190 175 93 89
74 62 51
BspEI T'CCGG_A
Cuts at: 2930 2930
Size: 3710
BspHI T'CATG_A
Cuts at: 2886 2886
Size: 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 760 974 1276 1657 760
Size: 214 302 381 2813
Fragments arranged by size:
2813 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2002 2056 3540 3705 50
Size: 1952 54 1484 165 55
Fragments arranged by size:
1952 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 274 1622 274
Size: 1348 2362
BsrFI r'CCGG_y
Cuts at: 239 1708 1889 2098 239
Size: 1469 181 209 1851
Fragments arranged by size:
1851 1469 209 181
BssSI C'ACGA_G
Cuts at: 1981 2339 1981
Size: 358 3352
BstAPI GCAn_nnn'nTGC
Cuts at: 1087 1087
Size: 3710
BstBI TT'CG_AA
Cuts at: 2071 2071
Size: 3710
BstXI CCAn_nnnn'nTGG
Cuts at: 459 459
Size: 3710
BstYI r'GATC_y
Cuts at: 1 735 1202 1225 1560 1806 2818 2904 2916
Size: 734 467 23 335 246 1012 86 12
Cuts at: 2916 3262 1
Size: 346 449
Fragments arranged by size:
1012 734 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1660 1841 2004 1660
Size: 181 163 3366
Fragments arranged by size:
3366 181 163
BtsI GCAGTG_nn'
Cuts at: 953 953
Size: 3710
DraI TTT'AAA
Cuts at: 709 709
Size: 3710
DrdI GACnn_nn'nnGTC
Cuts at: 1416 2274 1416
Size: 858 2852
EaeI y'GGCC_r
Cuts at: 137 314 1295 1469 1860 1887 3232 137
Size: 177 981 174 391 27 1345 615
Fragments arranged by size:
1345 981 615 391 177 174 27
EagI C'GGCC_G
Cuts at: 1295 1295
Size: 3710
EarI CTCTTCn'nnn_
Cuts at: 1733 1943 1733
Size: 210 3500
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 529 1918 2228 2374 3520 529
Size: 1389 310 146 1146 719
Fragments arranged by size:
1389 1146 719 310 146
EcoICRI GAG'CTC
Cuts at: 170 396 722 170
Size: 226 326 3158
Fragments arranged by size:
3158 326 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 631 1535 1967 2714 2982 619
Size: 12 904 432 747 268 1347
Fragments arranged by size:
1347 904 747 432 268 12
EcoO109I rG'GnC_Cy
Cuts at: 849 849
Size: 3710
EcoRI G'AATT_C
Cuts at: 714 714
Size: 3710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 561 1729 561
Size: 1168 2542
FspI TGC'GCA
Cuts at: 1491 1491
Size: 3710
HaeII r_GCGC'y
Cuts at: 1144 1392 2414 3393 1144
Size: 248 1022 979 1461
Fragments arranged by size:
1461 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 615 647 1552 1582 1584 1614 615
Size: 32 905 30 2 30 2711
Fragments arranged by size:
2711 905 32 30 30 2
HincII GTy'rAC
Cuts at: 749 749
Size: 3710
KasI G'GCGC_C
Cuts at: 1140 1388 1140
Size: 248 3462
KpnI G_GTAC'C
Cuts at: 730 730
Size: 3710
MluI A'CGCG_T
Cuts at: 3143 3143
Size: 3710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2565 2381
Size: 184 3526
MscI TGG'CCA
Cuts at: 1471 1471
Size: 3710
MslI CAynn'nnrTG
Cuts at: 358 460 537 1699 1826 358
Size: 102 77 1162 127 2242
Fragments arranged by size:
2242 1162 127 102 77
MspA1I CmG'CkG
Cuts at: 254 324 821 1135 1495 2508 2753 254
Size: 70 497 314 360 1013 245 1211
Fragments arranged by size:
1211 1013 497 360 314 245 70
NaeI GCC'GGC
Cuts at: 1891 1891
Size: 3710
NarI GG'CG_CC
Cuts at: 1141 1389 1141
Size: 248 3462
NdeI CA'TA_TG
Cuts at: 268 268
Size: 3710
NgoMIV G'CCGG_C
Cuts at: 1889 1889
Size: 3710
NspI r_CATG'y
Cuts at: 763 1263 2170 763
Size: 500 907 2303
Fragments arranged by size:
2303 907 500
PciI A'CATG_T
Cuts at: 2166 2166
Size: 3710
PfoI T'CCnGG_A
Cuts at: 203 593 203
Size: 390 3320
PmeI GTTT'AAAC
Cuts at: 709 709
Size: 3710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 3678
PstI C_TGCA'G
Cuts at: 757 1442 757
Size: 685 3025
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3710
PvuII CAG'CTG
Cuts at: 254 1135 1495 254
Size: 881 360 2469
Fragments arranged by size:
2469 881 360
RsrII CG'GwC_CG
Cuts at: 1905 1905
Size: 3710
SacI G_AGCT'C
Cuts at: 172 398 724 172
Size: 226 326 3158
Fragments arranged by size:
3158 326 226
SalI G'TCGA_C
Cuts at: 747 747
Size: 3710
SapI GCTCTTCn'nnn_
Cuts at: 1733 1943 1733
Size: 210 3500
SbfI CC_TGCA'GG
Cuts at: 757 757
Size: 3710
ScaI AGT'ACT
Cuts at: 3227 3227
Size: 3710
SfcI C'TryA_G
Cuts at: 33 753 1438 2431 2622 33
Size: 720 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 191
SfoI GGC'GCC
Cuts at: 1142 1390 1142
Size: 248 3462
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
SmaI CCC'GGG
Cuts at: 732 732
Size: 3710
SmlI C'TyrA_G
Cuts at: 397 865 1780 2272 2534 397
Size: 468 915 492 262 1573
Fragments arranged by size:
1573 915 492 468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3710
SphI G_CATG'C
Cuts at: 763 1263 763
Size: 500 3210
StuI AGG'CCT
Cuts at: 520 520
Size: 3710
StyI C'CwwG_G
Cuts at: 386 521 844 1198 386
Size: 135 323 354 2898
Fragments arranged by size:
2898 354 323 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1293 1426 1885 2094 1293
Size: 133 459 209 2909
Fragments arranged by size:
2909 459 209 133
TatI w'GTAC_w
Cuts at: 362 3225 362
Size: 2863 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 459 491 3227 3259 459
Size: 32 2736 32 910
Fragments arranged by size:
2736 910 32 32
Tth111I GACn'n_nGTC
Cuts at: 1507 1507
Size: 3710
XbaI T'CTAG_A
Cuts at: 741 741
Size: 3710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 1205 459
Size: 746 2964
XmaI C'CCGG_G
Cuts at: 730 730
Size: 3710
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI