Restriction map for
pF1KB8756
(Circular) (Six-base) MAPSORT of: pF1KB8756.seq Check: 294 from: 1 to: 3713
pF1KB8756 3713 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 751 751
Size: 3713
Acc65I G'GTAC_C
Cuts at: 729 729
Size: 3713
AclI AA'CG_TT
Cuts at: 3553 3553
Size: 3713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 1970 2717 2985 1538
Size: 432 747 268 2266
Fragments arranged by size:
2266 747 432 268
AflIII A'CryG_T
Cuts at: 1065 2169 3146 1065
Size: 1104 977 1632
Fragments arranged by size:
1632 1104 977
AgeI A'CCGG_T
Cuts at: 2101 2101
Size: 3713
AlwNI CAG_nnn'CTG
Cuts at: 1018 2585 1018
Size: 1567 2146
ApaLI G'TGCA_C
Cuts at: 2483 2483
Size: 3713
ApoI r'AATT_y
Cuts at: 238 300 717 3066 3322 238
Size: 62 417 2349 256 629
Fragments arranged by size:
2349 629 417 256 62
AseI AT'TA_AT
Cuts at: 20 20
Size: 3713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
AvaI C'yCGr_G
Cuts at: 733 733
Size: 3713
BamHI G'GATC_C
Cuts at: 738 738
Size: 3713
BanI G'GyrC_C
Cuts at: 729 1143 1391 1426 729
Size: 414 248 35 3016
Fragments arranged by size:
3016 414 248 35
BanII G_rGCy'C
Cuts at: 580 727 1757 580
Size: 147 1030 2536
Fragments arranged by size:
2536 1030 147
BbeI G_GCGC'C
Cuts at: 1147 1395 1147
Size: 248 3465
BciVI GTATCCnnnnn_n'
Cuts at: 1611 2372 3713 1611
Size: 761 1341 1611
Fragments arranged by size:
1611 1341 761
BclI T'GATC_A
Cuts at: 662 1233 662
Size: 571 3142
BglII A'GATC_T
Cuts at: 1228 3265 1228
Size: 2037 1676
BlpI GC'TnA_GC
Cuts at: 825 825
Size: 3713
Bme1580I G_kGCm'C
Cuts at: 1338 1431 2487 1338
Size: 93 1056 2564
Fragments arranged by size:
2564 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 925 1006 1341 3485 925
Size: 81 335 2144 1153
Fragments arranged by size:
2144 1153 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 3713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 889 1768 2260 2558 664
Size: 225 879 492 298 1819
Fragments arranged by size:
1819 879 492 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 3417 3417
Size: 3713
BsaAI yAC'GTr
Cuts at: 1066 1066
Size: 3713
BsaBI GATnn'nnATC
Cuts at: 6 1227 3264 6
Size: 1221 2037 455
Fragments arranged by size:
2037 1221 455
BsaHI Gr'CG_yC
Cuts at: 1144 1392 2094 3590 1144
Size: 248 702 1496 1267
Fragments arranged by size:
1496 1267 702 248
BsaWI w'CCGG_w
Cuts at: 902 1423 2101 2375 2522 2933 902
Size: 521 678 274 147 411 1682
Fragments arranged by size:
1682 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 476 506 476
Size: 30 3683
BseYI C'CCAG_C
Cuts at: 1138 1896 2473 2969 1138
Size: 758 577 496 1882
Fragments arranged by size:
1882 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1255 1301 2509 104
Size: 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 46
BsiHKAI G_wGCw'C
Cuts at: 580 727 1505 1695 2487 580
Size: 147 778 190 792 1806
Fragments arranged by size:
1806 792 778 190 147
Bsp1286I G_dGCh'C
Cuts at: 580 727 1338 1431 1505 1695 1757 2487 580
Size: 147 611 93 74 190 62 730 1806
Fragments arranged by size:
1806 730 611 190 147 93 74 62
BspEI T'CCGG_A
Cuts at: 2933 2933
Size: 3713
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 158 763 977 1279 1660 158
Size: 605 214 302 381 2211
Fragments arranged by size:
2211 605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2005 2059 3543 3708 50
Size: 1955 54 1484 165 55
Fragments arranged by size:
1955 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 569 1625 569
Size: 1056 2657
BsrFI r'CCGG_y
Cuts at: 1711 1892 2101 1711
Size: 181 209 3323
Fragments arranged by size:
3323 209 181
BssSI C'ACGA_G
Cuts at: 1984 2342 1984
Size: 358 3355
BstAPI GCAn_nnn'nTGC
Cuts at: 1090 1090
Size: 3713
BstBI TT'CG_AA
Cuts at: 2074 2074
Size: 3713
BstEII G'GTnAC_C
Cuts at: 288 288
Size: 3713
BstYI r'GATC_y
Cuts at: 1 738 1205 1228 1563 1809 2821 2907 2919
Size: 737 467 23 335 246 1012 86 12
Cuts at: 2919 3265 1
Size: 346 449
Fragments arranged by size:
1012 737 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1663 1844 2007 1663
Size: 181 163 3369
Fragments arranged by size:
3369 181 163
BtsI GCAGTG_nn'
Cuts at: 956 956
Size: 3713
DraI TTT'AAA
Cuts at: 712 712
Size: 3713
DrdI GACnn_nn'nnGTC
Cuts at: 1419 2277 1419
Size: 858 2855
EaeI y'GGCC_r
Cuts at: 1298 1472 1863 1890 3235 1298
Size: 174 391 27 1345 1776
Fragments arranged by size:
1776 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1298 1298
Size: 3713
EarI CTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 2231 2377 3523 1921
Size: 310 146 1146 2111
Fragments arranged by size:
2111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 578 725 578
Size: 147 3566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 1970 2717 2985 1538
Size: 432 747 268 2266
Fragments arranged by size:
2266 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 260 260
Size: 3713
EcoO109I rG'GnC_Cy
Cuts at: 852 852
Size: 3713
EcoRI G'AATT_C
Cuts at: 717 717
Size: 3713
EcoRV GAT'ATC
Cuts at: 470 470
Size: 3713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 309 1732 309
Size: 1423 2290
FspI TGC'GCA
Cuts at: 1494 1494
Size: 3713
HaeII r_GCGC'y
Cuts at: 1147 1395 2417 3396 1147
Size: 248 1022 979 1464
Fragments arranged by size:
1464 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1555 1585 1587 1617 1555
Size: 30 2 30 3651
Fragments arranged by size:
3651 30 30 2
HincII GTy'rAC
Cuts at: 752 752
Size: 3713
KasI G'GCGC_C
Cuts at: 1143 1391 1143
Size: 248 3465
KpnI G_GTAC'C
Cuts at: 733 733
Size: 3713
MluI A'CGCG_T
Cuts at: 3146 3146
Size: 3713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 170 2384 2568 170
Size: 2214 184 1315
Fragments arranged by size:
2214 1315 184
MscI TGG'CCA
Cuts at: 1474 1474
Size: 3713
MslI CAynn'nnrTG
Cuts at: 1702 1829 1702
Size: 127 3586
MspA1I CmG'CkG
Cuts at: 824 1138 1498 2511 2756 824
Size: 314 360 1013 245 1781
Fragments arranged by size:
1781 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1894 1894
Size: 3713
NarI GG'CG_CC
Cuts at: 1144 1392 1144
Size: 248 3465
NcoI C'CATG_G
Cuts at: 107 107
Size: 3713
NgoMIV G'CCGG_C
Cuts at: 1892 1892
Size: 3713
NspI r_CATG'y
Cuts at: 766 1266 2173 766
Size: 500 907 2306
Fragments arranged by size:
2306 907 500
PciI A'CATG_T
Cuts at: 2169 2169
Size: 3713
PmeI GTTT'AAAC
Cuts at: 712 712
Size: 3713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 3681
PshAI GACnn'nnGTC
Cuts at: 211 211
Size: 3713
PstI C_TGCA'G
Cuts at: 230 760 1445 230
Size: 530 685 2498
Fragments arranged by size:
2498 685 530
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3713
PvuII CAG'CTG
Cuts at: 1138 1498 1138
Size: 360 3353
RsrII CG'GwC_CG
Cuts at: 1908 1908
Size: 3713
SacI G_AGCT'C
Cuts at: 580 727 580
Size: 147 3566
SalI G'TCGA_C
Cuts at: 750 750
Size: 3713
SapI GCTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
SbfI CC_TGCA'GG
Cuts at: 760 760
Size: 3713
ScaI AGT'ACT
Cuts at: 3230 3230
Size: 3713
SfcI C'TryA_G
Cuts at: 33 226 756 1441 2434 2625 33
Size: 193 530 685 993 191 1121
Fragments arranged by size:
1121 993 685 530 193 191
SfoI GGC'GCC
Cuts at: 1145 1393 1145
Size: 248 3465
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
SmaI CCC'GGG
Cuts at: 735 735
Size: 3713
SmlI C'TyrA_G
Cuts at: 643 868 1783 2275 2537 643
Size: 225 915 492 262 1819
Fragments arranged by size:
1819 915 492 262 225
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3713
SphI G_CATG'C
Cuts at: 766 1266 766
Size: 500 3213
StyI C'CwwG_G
Cuts at: 107 293 847 1201 107
Size: 186 554 354 2619
Fragments arranged by size:
2619 554 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 556 1296 1429 1888 2097 556
Size: 740 133 459 209 2172
Fragments arranged by size:
2172 740 459 209 133
TatI w'GTAC_w
Cuts at: 492 3228 492
Size: 2736 977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 3681
Tth111I GACn'n_nGTC
Cuts at: 1510 1510
Size: 3713
XbaI T'CTAG_A
Cuts at: 744 744
Size: 3713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1208 1208
Size: 3713
XmaI C'CCGG_G
Cuts at: 733 733
Size: 3713
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI