Restriction map for
pF1KB8757
(Circular) (Six-base) MAPSORT of: pF1KB8757.seq Check: 5927 from: 1 to: 3716
pF1KB8757 3716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 338 338
Size: 3716
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2720 2988 1541
Size: 432 747 268 2269
Fragments arranged by size:
2269 747 432 268
AfeI AGC'GCT
Cuts at: 540 615 540
Size: 75 3641
AflIII A'CryG_T
Cuts at: 1068 2172 3149 1068
Size: 1104 977 1635
Fragments arranged by size:
1635 1104 977
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AhdI GACnn_n'nnGTC
Cuts at: 392 392
Size: 3716
AleI CACnn'nnGTG
Cuts at: 598 598
Size: 3716
AlwNI CAG_nnn'CTG
Cuts at: 1021 2588 1021
Size: 1567 2149
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 720 3069 3325 720
Size: 2349 256 1111
Fragments arranged by size:
2349 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 298 526 689 736 298
Size: 228 163 47 3278
Fragments arranged by size:
3278 228 163 47
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 132 291 732 1146 1394 1429 132
Size: 159 441 414 248 35 2419
Fragments arranged by size:
2419 441 414 248 159 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 136 1150 1398 136
Size: 1014 248 2454
Fragments arranged by size:
2454 1014 248
BbvCI CC'TCA_GC
Cuts at: 545 545
Size: 3716
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 1231 3268 1231
Size: 2037 1679
BlpI GC'TnA_GC
Cuts at: 425 828 425
Size: 403 3313
Bme1580I G_kGCm'C
Cuts at: 296 534 1341 1434 2490 296
Size: 238 807 93 1056 1522
Fragments arranged by size:
1522 1056 807 238 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BmtI G_CTAG'C
Cuts at: 580 580
Size: 3716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 454
Size: 3716
Bpu10I CC'TnA_GC
Cuts at: 545 545
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 655 667 892 1771 2263 2561 214
Size: 441 12 225 879 492 298 1369
Fragments arranged by size:
1369 879 492 441 298 225 12
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 1069 1069
Size: 3716
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 133 335 1147 1395 2097 3593 133
Size: 202 812 248 702 1496 256
Fragments arranged by size:
1496 812 702 256 248 202
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 135 135
Size: 3716
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1258 1304 2512 104
Size: 1154 46 1208 1308
Fragments arranged by size:
1308 1208 1154 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 699 699
Size: 3716
Bsp1286I G_dGCh'C
Cuts at: 296 534 730 1341 1434 1508 1698 1760 2490
Size: 238 196 611 93 74 190 62 730
Cuts at: 2490 296
Size: 1522
Fragments arranged by size:
1522 730 611 238 196 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2819
Fragments arranged by size:
2819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 429 2008 2062 3546 3711 50
Size: 379 1579 54 1484 165 55
Fragments arranged by size:
1579 1484 379 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 1714 1895 2104 1714
Size: 181 209 3326
Fragments arranged by size:
3326 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 284 741 1208 1231 1566 1812 2824 2910
Size: 283 457 467 23 335 246 1012 86
Cuts at: 2910 2922 3268 1
Size: 12 346 449
Fragments arranged by size:
1012 467 457 449 346 335 283 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 137 959 137
Size: 822 2894
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 506 1301 1475 1866 1893 3238 506
Size: 795 174 391 27 1345 984
Fragments arranged by size:
1345 984 795 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 550 697 1739 1949 550
Size: 147 1042 210 2317
Fragments arranged by size:
2317 1042 210 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1541 1973 2720 2988 454
Size: 1087 432 747 268 1182
Fragments arranged by size:
1182 1087 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 637 637
Size: 3716
EcoO109I rG'GnC_Cy
Cuts at: 642 855 642
Size: 213 3503
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 136 372 542 617 654 1150 1398 2420 3399
Size: 236 170 75 37 496 248 1022 979
Cuts at: 3399 136
Size: 453
Fragments arranged by size:
1022 979 496 453 248 236 170 75
37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 378 410 1558 1588 1590 1620 378
Size: 32 1148 30 2 30 2474
Fragments arranged by size:
2474 1148 32 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 132 1146 1394 132
Size: 1014 248 2454
Fragments arranged by size:
2454 1014 248
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 508 1477 508
Size: 969 2747
MslI CAynn'nnrTG
Cuts at: 598 1705 1832 598
Size: 1107 127 2482
Fragments arranged by size:
2482 1107 127
MspA1I CmG'CkG
Cuts at: 827 1141 1501 2514 2759 827
Size: 314 360 1013 245 1784
Fragments arranged by size:
1784 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 133 1147 1395 133
Size: 1014 248 2454
Fragments arranged by size:
2454 1014 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NheI G'CTAG_C
Cuts at: 576 576
Size: 3716
NspI r_CATG'y
Cuts at: 178 769 1269 2176 178
Size: 591 500 907 1718
Fragments arranged by size:
1718 907 591 500
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3684
PstI C_TGCA'G
Cuts at: 613 638 763 1448 613
Size: 25 125 685 2881
Fragments arranged by size:
2881 685 125 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 609 634 759 1444 2437 2628 33
Size: 576 25 125 685 993 191 1121
Fragments arranged by size:
1121 993 685 576 191 125 25
SfoI GGC'GCC
Cuts at: 134 1148 1396 134
Size: 1014 248 2454
Fragments arranged by size:
2454 1014 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 528 738 528
Size: 210 3506
SmlI C'TyrA_G
Cuts at: 229 646 670 871 1786 2278 2540 229
Size: 417 24 201 915 492 262 1405
Fragments arranged by size:
1405 915 492 417 262 201 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 107 443 850 1204 107
Size: 336 407 354 2619
Fragments arranged by size:
2619 407 354 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 526 736 526
Size: 210 3506
ZraI GAC'GTC
Cuts at: 336 336
Size: 3716
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AflII AlfI AloI ApaI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BsaXI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI