Restriction map for
pF1KB8759
(Circular) (Six-base) MAPSORT of: pF1KB8759.seq Check: 1788 from: 1 to: 3716
pF1KB8759 3716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 609 609
Size: 3716
AccI GT'mk_AC
Cuts at: 485 754 485
Size: 269 3447
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 309 3556 309
Size: 3247 469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 1541 1973 2720 2988 351
Size: 1190 432 747 268 1079
Fragments arranged by size:
1190 1079 747 432 268
AflIII A'CryG_T
Cuts at: 1068 2172 3149 1068
Size: 1104 977 1635
Fragments arranged by size:
1635 1104 977
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 369 369
Size: 3716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 670 702 670
Size: 32 3684
AlwNI CAG_nnn'CTG
Cuts at: 624 1021 2588 624
Size: 397 1567 1752
Fragments arranged by size:
1752 1567 397
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 199 418 720 3069 3325 199
Size: 219 302 2349 256 590
Fragments arranged by size:
2349 590 302 256 219
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 736 736
Size: 3716
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 572 605 572
Size: 33 3683
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 732 1146 1394 1429 732
Size: 414 248 35 3019
Fragments arranged by size:
3019 414 248 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BbsI GAAGACnn'nnnn_
Cuts at: 692 692
Size: 3716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 683 717 683
Size: 34 3682
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 249 284 1231 3268 249
Size: 35 947 2037 697
Fragments arranged by size:
2037 947 697 35
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 1341 1434 2490 1341
Size: 93 1056 2567
Fragments arranged by size:
2567 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 274 664 265
Size: 9 390 3317
Fragments arranged by size:
3317 390 9
Bpu10I CC'TnA_GC
Cuts at: 449 449
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 189 1069 189
Size: 880 2836
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 670
Size: 30 3686
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 529
Size: 3716
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1258 1304 2512 104
Size: 1154 46 1208 1308
Fragments arranged by size:
1308 1208 1154 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 730 1341 1434 1508 1698 1760 2490 730
Size: 611 93 74 190 62 730 1956
Fragments arranged by size:
1956 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 609 766 980 1282 1663 609
Size: 157 214 302 381 2662
Fragments arranged by size:
2662 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 648 1628 648
Size: 980 2736
BsrFI r'CCGG_y
Cuts at: 1714 1895 2104 1714
Size: 181 209 3326
Fragments arranged by size:
3326 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 249 284 741 1208 1231 1566 1812 2824
Size: 248 35 457 467 23 335 246 1012
Cuts at: 2824 2910 2922 3268 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 457 449 346 335 248 246
86 35 23 12
Bsu36I CC'TnA_GG
Cuts at: 322 322
Size: 3716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 293 959 293
Size: 666 3050
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 1301 1475 1866 1893 3238 1301
Size: 174 391 27 1345 1779
Fragments arranged by size:
1779 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 274 351 664 1541 1973 2720 2988 265
Size: 9 77 313 877 432 747 268 993
Fragments arranged by size:
993 877 747 432 313 268 77 9
EcoNI CCTnn'n_nnAGG
Cuts at: 336 336
Size: 3716
EcoO109I rG'GnC_Cy
Cuts at: 444 855 444
Size: 411 3305
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 1150 1398 2420 3399 1150
Size: 248 1022 979 1467
Fragments arranged by size:
1467 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1588 1590 1620 1558
Size: 30 2 30 3654
Fragments arranged by size:
3654 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3716
MslI CAynn'nnrTG
Cuts at: 1705 1832 1705
Size: 127 3589
MspA1I CmG'CkG
Cuts at: 642 827 1141 1501 2514 2759 642
Size: 185 314 360 1013 245 1599
Fragments arranged by size:
1599 1013 360 314 245 185
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NspI r_CATG'y
Cuts at: 657 769 1269 2176 657
Size: 112 500 907 2197
Fragments arranged by size:
2197 907 500 112
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 670 702 2855 2887 670
Size: 32 2153 32 1499
Fragments arranged by size:
2153 1499 32 32
PpuMI rG'GwC_Cy
Cuts at: 444 444
Size: 3716
PstI C_TGCA'G
Cuts at: 300 763 1448 300
Size: 463 685 2568
Fragments arranged by size:
2568 685 463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 642 1141 1501 642
Size: 499 360 2857
Fragments arranged by size:
2857 499 360
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SexAI A'CCwGG_T
Cuts at: 526 526
Size: 3716
SfcI C'TryA_G
Cuts at: 33 296 336 629 759 1444 2437 2628 33
Size: 263 40 293 130 685 993 191 1121
Fragments arranged by size:
1121 993 685 293 263 191 130 40
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SnaBI TAC'GTA
Cuts at: 189 189
Size: 3716
SpeI A'CTAG_T
Cuts at: 67 565 67
Size: 498 3218
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 850 1204 850
Size: 354 3362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 136 1299 1432 1891 2100 136
Size: 1163 133 459 209 1752
Fragments arranged by size:
1752 1163 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BbvCI BfrBI BglI BmgBI BmtI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI