Restriction map for
pF1KB8761
(Circular) (Six-base) MAPSORT of: pF1KB8761.seq Check: 2890 from: 1 to: 3731
pF1KB8761 3731 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 747 747
Size: 3731
AclI AA'CG_TT
Cuts at: 3571 3571
Size: 3731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 452 1556 1988 2735 3003 362
Size: 90 1104 432 747 268 1090
Fragments arranged by size:
1104 1090 747 432 268 90
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 2501 2501
Size: 3731
ApoI r'AATT_y
Cuts at: 735 3084 3340 735
Size: 2349 256 1126
Fragments arranged by size:
2349 1126 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 751 751
Size: 3731
BamHI G'GATC_C
Cuts at: 636 756 636
Size: 120 3611
BanI G'GyrC_C
Cuts at: 747 1161 1409 1444 747
Size: 414 248 35 3034
Fragments arranged by size:
3034 414 248 35
BanII G_rGCy'C
Cuts at: 573 745 1775 573
Size: 172 1030 2529
Fragments arranged by size:
2529 1030 172
BbeI G_GCGC'C
Cuts at: 1165 1413 1165
Size: 248 3483
BbsI GAAGACnn'nnnn_
Cuts at: 253 253
Size: 3731
BciVI GTATCCnnnnn_n'
Cuts at: 1629 2390 3731 1629
Size: 761 1341 1629
Fragments arranged by size:
1629 1341 761
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglI GCCn_nnn'nGGC
Cuts at: 385 385
Size: 3731
BglII A'GATC_T
Cuts at: 1246 3283 1246
Size: 2037 1694
BlpI GC'TnA_GC
Cuts at: 843 843
Size: 3731
Bme1580I G_kGCm'C
Cuts at: 446 1356 1449 2505 446
Size: 910 93 1056 1672
Fragments arranged by size:
1672 1056 910 93
BmrI ACTGGGnnnn_n'
Cuts at: 943 1024 1359 3503 943
Size: 81 335 2144 1171
Fragments arranged by size:
2144 1171 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 3731
Bpu10I CC'TnA_GC
Cuts at: 572 583 572
Size: 11 3720
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1786 2278 2576 907
Size: 879 492 298 2062
Fragments arranged by size:
2062 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3435 3435
Size: 3731
BsaAI yAC'GTr
Cuts at: 1084 1084
Size: 3731
BsaBI GATnn'nnATC
Cuts at: 6 1245 3282 6
Size: 1239 2037 455
Fragments arranged by size:
2037 1239 455
BsaHI Gr'CG_yC
Cuts at: 1162 1410 2112 3608 1162
Size: 248 702 1496 1285
Fragments arranged by size:
1496 1285 702 248
BsaWI w'CCGG_w
Cuts at: 920 1441 2119 2393 2540 2951 920
Size: 521 678 274 147 411 1700
Fragments arranged by size:
1700 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 592 550
Size: 42 3689
BseYI C'CCAG_C
Cuts at: 1156 1914 2491 2987 1156
Size: 758 577 496 1900
Fragments arranged by size:
1900 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 745 1523 1713 2505 745
Size: 778 190 792 1971
Fragments arranged by size:
1971 792 778 190
BsmI GAATG_Cn'
Cuts at: 259 259
Size: 3731
Bsp1286I G_dGCh'C
Cuts at: 446 573 745 1356 1449 1523 1713 1775 2505
Size: 127 172 611 93 74 190 62 730
Cuts at: 2505 446
Size: 1672
Fragments arranged by size:
1672 730 611 190 172 127 93 74
62
BspEI T'CCGG_A
Cuts at: 2951 2951
Size: 3731
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 781 995 1297 1678 781
Size: 214 302 381 2834
Fragments arranged by size:
2834 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2023 2077 3561 3726 50
Size: 1973 54 1484 165 55
Fragments arranged by size:
1973 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1643 1643
Size: 3731
BsrFI r'CCGG_y
Cuts at: 1729 1910 2119 1729
Size: 181 209 3341
Fragments arranged by size:
3341 209 181
BsrGI T'GTAC_A
Cuts at: 630 630
Size: 3731
BssSI C'ACGA_G
Cuts at: 2002 2360 2002
Size: 358 3373
BstAPI GCAn_nnn'nTGC
Cuts at: 1108 1108
Size: 3731
BstBI TT'CG_AA
Cuts at: 2092 2092
Size: 3731
BstYI r'GATC_y
Cuts at: 1 636 756 1223 1246 1581 1827 2839 2925
Size: 635 120 467 23 335 246 1012 86
Cuts at: 2925 2937 3283 1
Size: 12 346 449
Fragments arranged by size:
1012 635 467 449 346 335 246 120
86 23 12
BtgI C'CryG_G
Cuts at: 107 406 107
Size: 299 3432
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1681 1862 2025 1681
Size: 181 163 3387
Fragments arranged by size:
3387 181 163
BtsI GCAGTG_nn'
Cuts at: 537 974 537
Size: 437 3294
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DraIII CAC_nnn'GTG
Cuts at: 364 364
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 1316 1490 1881 1908 3253 1316
Size: 174 391 27 1345 1794
Fragments arranged by size:
1794 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 698 1939 2249 2395 3541 698
Size: 1241 310 146 1146 888
Fragments arranged by size:
1241 1146 888 310 146
EcoICRI GAG'CTC
Cuts at: 743 743
Size: 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 452 1556 1988 2735 3003 362
Size: 90 1104 432 747 268 1090
Fragments arranged by size:
1104 1090 747 432 268 90
EcoO109I rG'GnC_Cy
Cuts at: 870 870
Size: 3731
EcoRI G'AATT_C
Cuts at: 735 735
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1750 1750
Size: 3731
FspI TGC'GCA
Cuts at: 1512 1512
Size: 3731
HaeII r_GCGC'y
Cuts at: 426 1165 1413 2435 3414 426
Size: 739 248 1022 979 743
Fragments arranged by size:
1022 979 743 739 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1573 1603 1605 1635 1573
Size: 30 2 30 3669
Fragments arranged by size:
3669 30 30 2
HincII GTy'rAC
Cuts at: 770 770
Size: 3731
KasI G'GCGC_C
Cuts at: 1161 1409 1161
Size: 248 3483
KpnI G_GTAC'C
Cuts at: 751 751
Size: 3731
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2586 2402
Size: 184 3547
MscI TGG'CCA
Cuts at: 1492 1492
Size: 3731
MslI CAynn'nnrTG
Cuts at: 1720 1847 1720
Size: 127 3604
MspA1I CmG'CkG
Cuts at: 408 842 1156 1516 2529 2774 408
Size: 434 314 360 1013 245 1365
Fragments arranged by size:
1365 1013 434 360 314 245
NaeI GCC'GGC
Cuts at: 1912 1912
Size: 3731
NarI GG'CG_CC
Cuts at: 1162 1410 1162
Size: 248 3483
NcoI C'CATG_G
Cuts at: 107 107
Size: 3731
NdeI CA'TA_TG
Cuts at: 720 720
Size: 3731
NgoMIV G'CCGG_C
Cuts at: 1910 1910
Size: 3731
NspI r_CATG'y
Cuts at: 784 1284 2191 784
Size: 500 907 2324
Fragments arranged by size:
2324 907 500
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 3699
PstI C_TGCA'G
Cuts at: 778 1463 778
Size: 685 3046
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 1156 1516 1156
Size: 360 3371
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 745 745
Size: 3731
SacII CC_GC'GG
Cuts at: 409 409
Size: 3731
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 778 778
Size: 3731
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SfcI C'TryA_G
Cuts at: 33 774 1459 2452 2643 33
Size: 741 685 993 191 1121
Fragments arranged by size:
1121 993 741 685 191
SfoI GGC'GCC
Cuts at: 1163 1411 1163
Size: 248 3483
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 886 1801 2293 2555 886
Size: 915 492 262 2062
Fragments arranged by size:
2062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3731
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StyI C'CwwG_G
Cuts at: 107 491 649 865 1219 107
Size: 384 158 216 354 2619
Fragments arranged by size:
2619 384 354 216 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 592 1314 1447 1906 2115 592
Size: 722 133 459 209 2208
Fragments arranged by size:
2208 722 459 209 133
TatI w'GTAC_w
Cuts at: 193 630 3246 193
Size: 437 2616 678
Fragments arranged by size:
2616 678 437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 3699
Tth111I GACn'n_nGTC
Cuts at: 1528 1528
Size: 3731
XbaI T'CTAG_A
Cuts at: 762 762
Size: 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1226 1226
Size: 3731
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI BsaXI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI