Restriction map for
pF1KB8762
(Circular) (Six-base) MAPSORT of: pF1KB8762.seq Check: 9280 from: 1 to: 3743
pF1KB8762 3743 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 347 1568 2000 2747 3015 154
Size: 193 1221 432 747 268 882
Fragments arranged by size:
1221 882 747 432 268 193
AflIII A'CryG_T
Cuts at: 257 1095 2199 3176 257
Size: 838 1104 977 824
Fragments arranged by size:
1104 977 838 824
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AhdI GACnn_n'nnGTC
Cuts at: 180 180
Size: 3743
AleI CACnn'nnGTG
Cuts at: 118 118
Size: 3743
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 415
Size: 32 3711
AlwNI CAG_nnn'CTG
Cuts at: 574 714 1048 2615 574
Size: 140 334 1567 1702
Fragments arranged by size:
1702 1567 334 140
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 747 3096 3352 747
Size: 2349 256 1138
Fragments arranged by size:
2349 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 763 763
Size: 3743
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 120 337 759 1173 1421 1456 120
Size: 217 422 414 248 35 2407
Fragments arranged by size:
2407 422 414 248 217 35
BanII G_rGCy'C
Cuts at: 395 623 757 1787 395
Size: 228 134 1030 2351
Fragments arranged by size:
2351 1030 228 134
BbeI G_GCGC'C
Cuts at: 1177 1425 1177
Size: 248 3495
BbvCI CC'TCA_GC
Cuts at: 521 521
Size: 3743
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 194 1263 194
Size: 1069 2674
BglII A'GATC_T
Cuts at: 1258 3295 1258
Size: 2037 1706
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 125 1368 1461 2517 125
Size: 1243 93 1056 1351
Fragments arranged by size:
1351 1243 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 955 1036 1371 3515 955
Size: 81 335 2144 1183
Fragments arranged by size:
2144 1183 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 594 2110 594
Size: 1516 2227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 579 532
Size: 47 3696
Bpu10I CC'TnA_GC
Cuts at: 521 521
Size: 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 919 1798 2290 2588 403
Size: 516 879 492 298 1558
Fragments arranged by size:
1558 879 516 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 508 3447 508
Size: 2939 804
BsaAI yAC'GTr
Cuts at: 660 1096 660
Size: 436 3307
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 1174 1422 2124 3620 1174
Size: 248 702 1496 1297
Fragments arranged by size:
1496 1297 702 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2405 2552 2963 932
Size: 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 415 445 533 563 415
Size: 30 88 30 3595
Fragments arranged by size:
3595 88 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 631 631
Size: 3743
BseYI C'CCAG_C
Cuts at: 207 1168 1926 2503 2999 207
Size: 961 758 577 496 951
Fragments arranged by size:
961 951 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 640 490
Size: 150 3593
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2539 104
Size: 1181 46 1208 1308
Fragments arranged by size:
1308 1208 1181 46
BsiHKAI G_wGCw'C
Cuts at: 395 757 1535 1725 2517 395
Size: 362 778 190 792 1621
Fragments arranged by size:
1621 792 778 362 190
Bsp1286I G_dGCh'C
Cuts at: 125 395 623 757 1368 1461 1535 1725 1787
Size: 270 228 134 611 93 74 190 62
Cuts at: 1787 2517 125
Size: 730 1351
Fragments arranged by size:
1351 730 611 270 228 190 134 93
74 62
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 185 793 1007 1309 1690 185
Size: 608 214 302 381 2238
Fragments arranged by size:
2238 608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1655 1655
Size: 3743
BsrFI r'CCGG_y
Cuts at: 1741 1922 2131 1741
Size: 181 209 3353
Fragments arranged by size:
3353 209 181
BssSI C'ACGA_G
Cuts at: 2014 2372 2014
Size: 358 3385
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstYI r'GATC_y
Cuts at: 1 728 768 1235 1258 1593 1839 2851 2937
Size: 727 40 467 23 335 246 1012 86
Cuts at: 2937 2949 3295 1
Size: 12 346 449
Fragments arranged by size:
1012 727 467 449 346 335 246 86
40 23 12
BtgI C'CryG_G
Cuts at: 311 311
Size: 3743
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3399
Fragments arranged by size:
3399 181 163
BtsI GCAGTG_nn'
Cuts at: 246 258 986 246
Size: 12 728 3003
Fragments arranged by size:
3003 728 12
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DraIII CAC_nnn'GTG
Cuts at: 380 380
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 603 1449 2307 603
Size: 846 858 2039
Fragments arranged by size:
2039 858 846
EaeI y'GGCC_r
Cuts at: 1328 1502 1893 1920 3265 1328
Size: 174 391 27 1345 1806
Fragments arranged by size:
1806 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 208 1951 2261 2407 3553 208
Size: 1743 310 146 1146 398
Fragments arranged by size:
1743 1146 398 310 146
EcoICRI GAG'CTC
Cuts at: 393 755 393
Size: 362 3381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 347 532 579 1568 2000 2747 3015 154
Size: 193 185 47 989 432 747 268 882
Fragments arranged by size:
989 882 747 432 268 193 185 47
EcoO109I rG'GnC_Cy
Cuts at: 174 322 670 711 882 174
Size: 148 348 41 171 3035
Fragments arranged by size:
3035 348 171 148 41
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3743
HaeII r_GCGC'y
Cuts at: 1177 1425 2447 3426 1177
Size: 248 1022 979 1494
Fragments arranged by size:
1494 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 391 423 690 722 1585 1615 1617
Size: 32 193 32 267 32 863 30 2
Cuts at: 1617 1647 166
Size: 30 2262
Fragments arranged by size:
2262 863 267 193 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
HindIII A'AGCT_T
Cuts at: 166 166
Size: 3743
KasI G'GCGC_C
Cuts at: 1173 1421 1173
Size: 248 3495
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2598 2414
Size: 184 3559
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 118 1732 1859 118
Size: 1614 127 2002
Fragments arranged by size:
2002 1614 127
MspA1I CmG'CkG
Cuts at: 207 854 1168 1528 2541 2786 207
Size: 647 314 360 1013 245 1164
Fragments arranged by size:
1164 1013 647 360 314 245
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3743
NarI GG'CG_CC
Cuts at: 1174 1422 1174
Size: 248 3495
NcoI C'CATG_G
Cuts at: 311 311
Size: 3743
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3743
NspI r_CATG'y
Cuts at: 261 726 796 1296 2203 261
Size: 465 70 500 907 1801
Fragments arranged by size:
1801 907 500 465 70
PciI A'CATG_T
Cuts at: 257 2199 257
Size: 1942 1801
PflMI CCAn_nnn'nTGG
Cuts at: 574 574
Size: 3743
PfoI T'CCnGG_A
Cuts at: 395 680 395
Size: 285 3458
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PmlI CAC'GTG
Cuts at: 660 660
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 415 447 2882 2914 415
Size: 32 2435 32 1244
Fragments arranged by size:
2435 1244 32 32
PpuMI rG'GwC_Cy
Cuts at: 174 711 174
Size: 537 3206
PstI C_TGCA'G
Cuts at: 284 698 790 1475 284
Size: 414 92 685 2552
Fragments arranged by size:
2552 685 414 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 1168 1528 1168
Size: 360 3383
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 395 757 395
Size: 362 3381
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 284 790 284
Size: 506 3237
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SfcI C'TryA_G
Cuts at: 33 280 694 786 1471 2464 2655 33
Size: 247 414 92 685 993 191 1121
Fragments arranged by size:
1121 993 685 414 247 191 92
SfoI GGC'GCC
Cuts at: 1175 1423 1175
Size: 248 3495
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 765 765
Size: 3743
SmlI C'TyrA_G
Cuts at: 418 898 1813 2305 2567 418
Size: 480 915 492 262 1594
Fragments arranged by size:
1594 915 492 480 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 726 796 1296 726
Size: 70 500 3173
Fragments arranged by size:
3173 500 70
StyI C'CwwG_G
Cuts at: 116 311 877 1231 116
Size: 195 566 354 2628
Fragments arranged by size:
2628 566 354 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 504 1326 1459 1918 2127 504
Size: 822 133 459 209 2120
Fragments arranged by size:
2120 822 459 209 133
TatI w'GTAC_w
Cuts at: 3258 3258
Size: 3743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 533 565 3260 3292 533
Size: 32 2695 32 984
Fragments arranged by size:
2695 984 32 32
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3743
XmaI C'CCGG_G
Cuts at: 763 763
Size: 3743
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI