Restriction map for
pF1KB8763
(Circular) (Six-base) MAPSORT of: pF1KB8763.seq Check: 7239 from: 1 to: 3746
pF1KB8763 3746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 636 1051 2618 636
Size: 415 1567 1764
Fragments arranged by size:
1764 1567 415
ApaI G_GGCC'C
Cuts at: 356 600 356
Size: 244 3502
ApaLI G'TGCA_C
Cuts at: 364 490 2516 364
Size: 126 2026 1594
Fragments arranged by size:
2026 1594 126
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 468 766 468
Size: 298 3448
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 403 576 762 1176 1424 1459 403
Size: 173 186 414 248 35 2690
Fragments arranged by size:
2690 414 248 186 173 35
BanII G_rGCy'C
Cuts at: 164 356 398 600 724 760 1790 164
Size: 192 42 202 124 36 1030 2120
Fragments arranged by size:
2120 1030 202 192 124 42 36
BbeI G_GCGC'C
Cuts at: 580 1180 1428 580
Size: 600 248 2898
Fragments arranged by size:
2898 600 248
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglI GCCn_nnn'nGGC
Cuts at: 168 168
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 235 356 368 406 494 600 1371 1464 2520
Size: 121 12 38 88 106 771 93 1056
Cuts at: 2520 235
Size: 1461
Fragments arranged by size:
1461 1056 771 121 106 93 88 38
12
BmrI ACTGGGnnnn_n'
Cuts at: 357 958 1039 1374 3518 357
Size: 601 81 335 2144 585
Fragments arranged by size:
2144 601 585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 580
Size: 3746
Bpu10I CC'TnA_GC
Cuts at: 358 451 718 358
Size: 93 267 3386
Fragments arranged by size:
3386 267 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 570 1099 570
Size: 529 3217
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 332 577 1177 1425 2127 3623 332
Size: 245 600 248 702 1496 455
Fragments arranged by size:
1496 702 600 455 248 245
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 706 736 706
Size: 30 3716
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 186 189 177
Size: 9 3 3734
Fragments arranged by size:
3734 9 3
BseYI C'CCAG_C
Cuts at: 591 1171 1929 2506 3002 591
Size: 580 758 577 496 1335
Fragments arranged by size:
1335 758 580 577 496
BsiEI CG_ry'CG
Cuts at: 104 229 507 625 1288 1334 2542 104
Size: 125 278 118 663 46 1208 1308
Fragments arranged by size:
1308 1208 663 278 125 118 46
BsiHKAI G_wGCw'C
Cuts at: 368 494 566 724 760 1538 1728 2520 368
Size: 126 72 158 36 778 190 792 1594
Fragments arranged by size:
1594 792 778 190 158 126 72 36
Bsp1286I G_dGCh'C
Cuts at: 164 235 356 368 398 406 494 566 600
Size: 71 121 12 30 8 88 72 34
Cuts at: 600 724 760 1371 1464 1538 1728 1790 2520
Size: 124 36 611 93 74 190 62 730
Cuts at: 2520 164
Size: 1390
Fragments arranged by size:
1390 730 611 190 124 121 93 88
74 72 71 62 36 34 30 12
8
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 381 2038 2092 3576 3741 50
Size: 331 1657 54 1484 165 55
Fragments arranged by size:
1657 1484 331 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 299 502 1744 1925 2134 299
Size: 203 1242 181 209 1911
Fragments arranged by size:
1911 1242 209 203 181
BssHII G'CGCG_C
Cuts at: 217 312 320 387 217
Size: 95 8 67 3576
Fragments arranged by size:
3576 95 67 8
BssSI C'ACGA_G
Cuts at: 376 477 2017 2375 376
Size: 101 1540 358 1747
Fragments arranged by size:
1747 1540 358 101
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2854 2940 2952
Size: 770 467 23 335 246 1012 86 12
Cuts at: 2952 3298 1
Size: 346 449
Fragments arranged by size:
1012 770 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 206 223 236 398 662 107
Size: 99 17 13 162 264 3191
Fragments arranged by size:
3191 264 162 99 17 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DraIII CAC_nnn'GTG
Cuts at: 570 570
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 142 1452 2310 142
Size: 1310 858 1578
Fragments arranged by size:
1578 1310 858
EaeI y'GGCC_r
Cuts at: 172 226 504 622 656 1331 1505 1896 1923
Size: 54 278 118 34 675 174 391 27
Cuts at: 1923 3268 172
Size: 1345 650
Fragments arranged by size:
1345 675 650 391 278 174 118 54
34 27
EagI C'GGCC_G
Cuts at: 226 504 622 1331 226
Size: 278 118 709 2641
Fragments arranged by size:
2641 709 278 118
EarI CTCTTCn'nnn_
Cuts at: 195 553 1769 1979 195
Size: 358 1216 210 1962
Fragments arranged by size:
1962 1216 358 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2264 2410 3556 1954
Size: 310 146 1146 2144
Fragments arranged by size:
2144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 722 758 722
Size: 36 3710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1571 2003 2750 3018 580
Size: 991 432 747 268 1308
Fragments arranged by size:
1308 991 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 352 353 472 596 597 885 352
Size: 1 119 124 1 288 3213
Fragments arranged by size:
3213 288 124 119 1 1
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FseI GG_CCGG'CC
Cuts at: 506 506
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 580 615 1180 1428 2450 3429 580
Size: 35 565 248 1022 979 897
Fragments arranged by size:
1022 979 897 565 248 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 576 1176 1424 576
Size: 600 248 2898
Fragments arranged by size:
2898 600 248
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 658 1507 658
Size: 849 2897
MslI CAynn'nnrTG
Cuts at: 487 518 1735 1862 487
Size: 31 1217 127 2371
Fragments arranged by size:
2371 1217 127 31
MspA1I CmG'CkG
Cuts at: 208 225 238 432 603 627 857 1171 1531
Size: 17 13 194 171 24 230 314 360
Cuts at: 1531 2544 2789 208
Size: 1013 245 1165
Fragments arranged by size:
1165 1013 360 314 245 230 194 171
24 17 13
NaeI GCC'GGC
Cuts at: 504 1927 504
Size: 1423 2323
NarI GG'CG_CC
Cuts at: 577 1177 1425 577
Size: 600 248 2898
Fragments arranged by size:
2898 600 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 502 1925 502
Size: 1423 2323
NotI GC'GGCC_GC
Cuts at: 622 622
Size: 3746
NspI r_CATG'y
Cuts at: 591 799 1299 2206 591
Size: 208 500 907 2131
Fragments arranged by size:
2131 907 500 208
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PfoI T'CCnGG_A
Cuts at: 326 326
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PpuMI rG'GwC_Cy
Cuts at: 472 472
Size: 3746
PspOMI G'GGCC_C
Cuts at: 352 596 352
Size: 244 3502
PstI C_TGCA'G
Cuts at: 632 793 1478 632
Size: 161 685 2900
Fragments arranged by size:
2900 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 1171 1531 1171
Size: 360 3386
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 724 760 724
Size: 36 3710
SacII CC_GC'GG
Cuts at: 209 226 239 209
Size: 17 13 3716
Fragments arranged by size:
3716 17 13
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SanDI GG'GwC_CC
Cuts at: 472 472
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SexAI A'CCwGG_T
Cuts at: 122 122
Size: 3746
SfcI C'TryA_G
Cuts at: 33 628 789 1474 2467 2658 33
Size: 595 161 685 993 191 1121
Fragments arranged by size:
1121 993 685 595 191 161
SfoI GGC'GCC
Cuts at: 578 1178 1426 578
Size: 600 248 2898
Fragments arranged by size:
2898 600 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 591 799 1299 591
Size: 208 500 3038
Fragments arranged by size:
3038 500 208
StyI C'CwwG_G
Cuts at: 107 880 1234 107
Size: 773 354 2619
Fragments arranged by size:
2619 773 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 1241 324
Size: 917 2829
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI