Restriction map for
pF1KB8765
(Circular) (Six-base) MAPSORT of: pF1KB8765.seq Check: 4567 from: 1 to: 3782
pF1KB8765 3782 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 480 480
Size: 3782
AccI GT'mk_AC
Cuts at: 290 820 290
Size: 530 3252
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
AflIII A'CryG_T
Cuts at: 1134 2238 3215 1134
Size: 1104 977 1701
Fragments arranged by size:
1701 1104 977
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AhdI GACnn_n'nnGTC
Cuts at: 161 161
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 402 744 1087 2654 402
Size: 342 343 1567 1530
Fragments arranged by size:
1567 1530 343 342
ApaI G_GGCC'C
Cuts at: 435 435
Size: 3782
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 202 786 3135 3391 202
Size: 584 2349 256 593
Fragments arranged by size:
2349 593 584 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 171 802 171
Size: 631 3151
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 407 798 1212 1460 1495 407
Size: 391 414 248 35 2694
Fragments arranged by size:
2694 414 391 248 35
BanII G_rGCy'C
Cuts at: 435 524 796 1826 435
Size: 89 272 1030 2391
Fragments arranged by size:
2391 1030 272 89
BbeI G_GCGC'C
Cuts at: 411 1216 1464 411
Size: 805 248 2729
Fragments arranged by size:
2729 805 248
BbvCI CC'TCA_GC
Cuts at: 403 403
Size: 3782
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BglI GCCn_nnn'nGGC
Cuts at: 509 509
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 435 1407 1500 2556 435
Size: 972 93 1056 1661
Fragments arranged by size:
1661 1056 972 93
BmrI ACTGGGnnnn_n'
Cuts at: 203 994 1075 1410 3554 203
Size: 791 81 335 2144 431
Fragments arranged by size:
2144 791 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 3782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 290 239
Size: 51 3731
Bpu10I CC'TnA_GC
Cuts at: 403 442 403
Size: 39 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 958 1837 2329 2627 490
Size: 468 879 492 298 1645
Fragments arranged by size:
1645 879 492 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 3486 3486
Size: 3782
BsaAI yAC'GTr
Cuts at: 1135 1135
Size: 3782
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 408 547 1213 1461 2163 3659 408
Size: 139 666 248 702 1496 531
Fragments arranged by size:
1496 702 666 531 248 139
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 247 340 541 597 621 665 1207 1965 2542
Size: 93 201 56 24 44 542 758 577
Cuts at: 2542 3038 247
Size: 496 991
Fragments arranged by size:
991 758 577 542 496 201 93 56
44 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 477 219
Size: 258 3524
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 796 1574 1764 2556 796
Size: 778 190 792 2022
Fragments arranged by size:
2022 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 435 524 796 1407 1500 1574 1764 1826 2556
Size: 89 272 611 93 74 190 62 730
Cuts at: 2556 435
Size: 1661
Fragments arranged by size:
1661 730 611 272 190 93 89 74
62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 480 832 1046 1348 1729 480
Size: 352 214 302 381 2533
Fragments arranged by size:
2533 381 352 302 214
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 1694
Size: 3782
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BssSI C'ACGA_G
Cuts at: 2053 2411 2053
Size: 358 3424
BstAPI GCAn_nnn'nTGC
Cuts at: 1159 1159
Size: 3782
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstEII G'GTnAC_C
Cuts at: 135 135
Size: 3782
BstXI CCAn_nnnn'nTGG
Cuts at: 255 255
Size: 3782
BstYI r'GATC_y
Cuts at: 1 807 1274 1297 1632 1878 2890 2976 2988
Size: 806 467 23 335 246 1012 86 12
Cuts at: 2988 3334 1
Size: 346 449
Fragments arranged by size:
1012 806 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 536 536
Size: 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 1025
Size: 3782
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 1367 1541 1932 1959 3304 1367
Size: 174 391 27 1345 1845
Fragments arranged by size:
1845 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 611 1805 2015 611
Size: 1194 210 2378
Fragments arranged by size:
2378 1194 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 290 1607 2039 2786 3054 239
Size: 51 1317 432 747 268 967
Fragments arranged by size:
1317 967 747 432 268 51
EcoO109I rG'GnC_Cy
Cuts at: 168 431 432 741 921 168
Size: 263 1 309 180 3029
Fragments arranged by size:
3029 309 263 180 1
EcoRI G'AATT_C
Cuts at: 202 786 202
Size: 584 3198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 298 1801 298
Size: 1503 2279
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 411 1216 1464 2486 3465 411
Size: 805 248 1022 979 728
Fragments arranged by size:
1022 979 805 728 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 147 179 358 390 537 569 1624 1654 1656
Size: 32 179 32 147 32 1055 30 2
Cuts at: 1656 1686 147
Size: 30 2243
Fragments arranged by size:
2243 1055 179 147 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 291 821 291
Size: 530 3252
KasI G'GCGC_C
Cuts at: 407 1212 1460 407
Size: 805 248 2729
Fragments arranged by size:
2729 805 248
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 706 2453 2637 706
Size: 1747 184 1851
Fragments arranged by size:
1851 1747 184
MscI TGG'CCA
Cuts at: 1543 1543
Size: 3782
MslI CAynn'nnrTG
Cuts at: 453 1771 1898 453
Size: 1318 127 2337
Fragments arranged by size:
2337 1318 127
MspA1I CmG'CkG
Cuts at: 597 893 1207 1567 2580 2825 597
Size: 296 314 360 1013 245 1554
Fragments arranged by size:
1554 1013 360 314 296 245
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 408 1213 1461 408
Size: 805 248 2729
Fragments arranged by size:
2729 805 248
NdeI CA'TA_TG
Cuts at: 482 482
Size: 3782
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NspI r_CATG'y
Cuts at: 348 835 1335 2242 348
Size: 487 500 907 1888
Fragments arranged by size:
1888 907 500 487
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PspOMI G'GGCC_C
Cuts at: 431 431
Size: 3782
PstI C_TGCA'G
Cuts at: 398 829 1514 398
Size: 431 685 2666
Fragments arranged by size:
2666 685 431
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 597 1207 1567 597
Size: 610 360 2812
Fragments arranged by size:
2812 610 360
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 289 819 289
Size: 530 3252
SapI GCTCTTCn'nnn_
Cuts at: 611 1805 2015 611
Size: 1194 210 2378
Fragments arranged by size:
2378 1194 210
SbfI CC_TGCA'GG
Cuts at: 398 829 398
Size: 431 3351
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 394 825 1510 2503 2694 33
Size: 361 431 685 993 191 1121
Fragments arranged by size:
1121 993 685 431 361 191
SfoI GGC'GCC
Cuts at: 409 1214 1462 409
Size: 805 248 2729
Fragments arranged by size:
2729 805 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 804 804
Size: 3782
SmlI C'TyrA_G
Cuts at: 505 937 1852 2344 2606 505
Size: 432 915 492 262 1681
Fragments arranged by size:
1681 915 492 432 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StuI AGG'CCT
Cuts at: 712 712
Size: 3782
StyI C'CwwG_G
Cuts at: 563 916 1270 563
Size: 353 354 3075
Fragments arranged by size:
3075 354 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1365 1498 1957 2166 1365
Size: 133 459 209 2981
Fragments arranged by size:
2981 459 209 133
TatI w'GTAC_w
Cuts at: 326 3297 326
Size: 2971 811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 228 260 3299 3331 228
Size: 32 3039 32 679
Fragments arranged by size:
3039 679 32 32
Tth111I GACn'n_nGTC
Cuts at: 141 1579 141
Size: 1438 2344
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 1277 214
Size: 1063 2719
XmaI C'CCGG_G
Cuts at: 802 802
Size: 3782
XmnI GAAnn'nnTTC
Cuts at: 733 733
Size: 3782
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI