Restriction map for
pF1KB8766
(Circular) (Six-base) MAPSORT of: pF1KB8766.seq Check: 3382 from: 1 to: 3788
pF1KB8766 3788 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 402 402
Size: 3788
AccI GT'mk_AC
Cuts at: 112 826 112
Size: 714 3074
Acc65I G'GTAC_C
Cuts at: 163 804 163
Size: 641 3147
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 1613 2045 2792 3060 350
Size: 1263 432 747 268 1078
Fragments arranged by size:
1263 1078 747 432 268
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaI G_GGCC'C
Cuts at: 626 626
Size: 3788
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 742 792 3141 3397 742
Size: 50 2349 256 1133
Fragments arranged by size:
2349 1133 256 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 163 804 1218 1466 1501 163
Size: 641 414 248 35 2450
Fragments arranged by size:
2450 641 414 248 35
BanII G_rGCy'C
Cuts at: 187 470 626 802 1832 187
Size: 283 156 176 1030 2143
Fragments arranged by size:
2143 1030 283 176 156
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 400 434 400
Size: 34 3754
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BfrBI ATG'CAT
Cuts at: 556 556
Size: 3788
BglII A'GATC_T
Cuts at: 398 1303 3340 398
Size: 905 2037 846
Fragments arranged by size:
2037 905 846
BlpI GC'TnA_GC
Cuts at: 213 900 213
Size: 687 3101
Bme1580I G_kGCm'C
Cuts at: 314 626 1413 1506 2562 314
Size: 312 787 93 1056 1540
Fragments arranged by size:
1540 1056 787 312 93
BmrI ACTGGGnnnn_n'
Cuts at: 311 1000 1081 1416 3560 311
Size: 689 81 335 2144 539
Fragments arranged by size:
2144 689 539 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 222 2155 222
Size: 1933 1855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 484 688 403
Size: 81 204 3503
Fragments arranged by size:
3503 204 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1843 2335 2633 964
Size: 879 492 298 2119
Fragments arranged by size:
2119 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 487 1141 487
Size: 654 3134
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 559
Size: 3788
BseYI C'CCAG_C
Cuts at: 262 1213 1971 2548 3044 262
Size: 951 758 577 496 1006
Fragments arranged by size:
1006 951 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 509
Size: 3788
BsiEI CG_ry'CG
Cuts at: 104 258 1330 1376 2584 104
Size: 154 1072 46 1208 1308
Fragments arranged by size:
1308 1208 1072 154 46
BsiHKAI G_wGCw'C
Cuts at: 470 802 1580 1770 2562 470
Size: 332 778 190 792 1696
Fragments arranged by size:
1696 792 778 332 190
Bsp1286I G_dGCh'C
Cuts at: 187 314 470 626 802 1413 1506 1580 1770
Size: 127 156 156 176 611 93 74 190
Cuts at: 1770 1832 2562 187
Size: 62 730 1413
Fragments arranged by size:
1413 730 611 190 176 156 156 127
93 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 302 402 838 1052 1354 1735 302
Size: 100 436 214 302 381 2355
Fragments arranged by size:
2355 436 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 1786 1967 2176 1786
Size: 181 209 3398
Fragments arranged by size:
3398 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 432 1165 432
Size: 733 3055
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 159 159
Size: 3788
BstYI r'GATC_y
Cuts at: 1 398 813 1280 1303 1638 1884 2896 2982
Size: 397 415 467 23 335 246 1012 86
Cuts at: 2982 2994 3340 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 415 397 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 169 169
Size: 3788
BtgI C'CryG_G
Cuts at: 227 227
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 660 1031 660
Size: 371 3417
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DraIII CAC_nnn'GTG
Cuts at: 779 779
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 1373 1547 1938 1965 3310 1373
Size: 174 391 27 1345 1851
Fragments arranged by size:
1851 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 468 800 468
Size: 332 3456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 403 484 688 1613 2045 2792 3060 350
Size: 53 81 204 925 432 747 268 1078
Fragments arranged by size:
1078 925 747 432 268 204 81 53
EcoO109I rG'GnC_Cy
Cuts at: 570 623 927 570
Size: 53 304 3431
Fragments arranged by size:
3431 304 53
EcoRI G'AATT_C
Cuts at: 742 792 742
Size: 50 3738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 298 1569 298
Size: 1271 2517
HaeII r_GCGC'y
Cuts at: 1222 1470 2492 3471 1222
Size: 248 1022 979 1539
Fragments arranged by size:
1539 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 1630 1660 1662 1692 400
Size: 32 1198 30 2 30 2496
Fragments arranged by size:
2496 1198 32 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 167 808 167
Size: 641 3147
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 899 1213 1573 2586 2831 899
Size: 314 360 1013 245 1856
Fragments arranged by size:
1856 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NsiI A_TGCA'T
Cuts at: 558 558
Size: 3788
NspI r_CATG'y
Cuts at: 393 841 1341 2248 393
Size: 448 500 907 1933
Fragments arranged by size:
1933 907 500 448
PasI CC'CwG_GG
Cuts at: 159 687 159
Size: 528 3260
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PfoI T'CCnGG_A
Cuts at: 284 284
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PmlI CAC'GTG
Cuts at: 487 487
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 334 366 2927 2959 334
Size: 32 2561 32 1163
Fragments arranged by size:
2561 1163 32 32
PpuMI rG'GwC_Cy
Cuts at: 570 570
Size: 3788
PspOMI G'GGCC_C
Cuts at: 622 622
Size: 3788
PstI C_TGCA'G
Cuts at: 587 835 1520 587
Size: 248 685 2855
Fragments arranged by size:
2855 685 248
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 1213 1573 1213
Size: 360 3428
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 470 802 470
Size: 332 3456
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SexAI A'CCwGG_T
Cuts at: 775 775
Size: 3788
SfcI C'TryA_G
Cuts at: 33 450 583 831 1516 2509 2700 33
Size: 417 133 248 685 993 191 1121
Fragments arranged by size:
1121 993 685 417 248 191 133
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 943 1858 2350 2612 943
Size: 915 492 262 2119
Fragments arranged by size:
2119 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StuI AGG'CCT
Cuts at: 223 223
Size: 3788
StyI C'CwwG_G
Cuts at: 218 922 1276 218
Size: 704 354 2730
Fragments arranged by size:
2730 704 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 340 3305 3337 308
Size: 32 2965 32 759
Fragments arranged by size:
2965 759 32 32
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1283 1283
Size: 3788
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
XmnI GAAnn'nnTTC
Cuts at: 598 598
Size: 3788
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PflMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI